스쿠알렌 합성 유전자를 도입한 형질전환 가시오가피

서진욱,정재훈,최용의,이학성,신차균 중앙대학교 유전공학연구소 2003 遺傳工學硏究論集 Vol.16 No.1

The enzyme, squalene synthase, represents a branch point in the isoprenoid pathway capable of diverting carbon flow specifically to the biosynthesis of phytosterol and Triterpenoid. Transgenic Eleutherococcus senticosus plants were prepared by introducing the genes for squalene synthase derived from Panax ginseng (PgSS1), hygromycin phosphotransferase (HPT) and green fluorescent protein (GFP) through Agrobacterium-mediated transformation. Establishment of transgenic plantlets were confirmed by the presence of PgSS1 and HPT bands in the genomic DNA preparation using a PCR method and a Southern blotting. In addition, expression of the introduced GFP DNA was confirmed by observing green fluorescence of GFP from the embroyos. In the in vitro analysis of the PgSS1 enzymatic activities the transgenic plants showed to have 1.5 to 3 times higher than wild type plant, indicating that the PgSS1 genes was well over-expressed in the transgenic plants. Especially the PgSS1 acivities of embroyogenic cell was 120 times higher than those of plantlet.

First Report of Endophytic Fungi, Aequabiliella effusa, Isolated from Twigs of Larix gmelinii var. olgensis in Korea

( Jae-eui Cha ),( Yun-jeong Kim ),( Ahn-heum Eom ) 한국균학회 2024 韓國菌學會誌 Vol.52 No.1

We isolated endophytic fungal strains from twigs of Larix gmelinii var. olgensis. These strains were identified based on their morphological characteristics and phylogenetic analyses including internal transcribed spacers and large subunit rDNA. We confirmed the presence of a previously unrecorded fungal species in Korea, Aequabiliella effusa. This study presents the morphological characteristics and phylogenetic analysis of A. effusa.

Asymbiotic Spore Production of Rhizoglomus intraradices in a Medium Containing Myristate

Cha Jae-Eui,Eom Ahn-Heum 한국균학회 2023 Mycobiology Vol.51 No.3

This study examined the effects of myristate on an asymbiotic culture of Rhizoglomus intraradices, a species of arbuscular mycorrhizal fungi (AMF; Glomeromycota). Mycelial growth and sporulation in a modified medium containing myristate were observed. The findings demonstrated that myristate induced R. intraradices spore formation, with daughter spores having a smaller diameter than the parent spores. This observation is consistent with previous studies on other Rhizoglomus species. Further studies are needed to investigate the potential for continuous culture, mass production using daughter spores, and the application of AMF colonization techniques in plants.

Three Unrecorded Endophytic Fungal Species Isolated from Plants in Taean-gun, Korea

Jae-Eui Cha,Hyeok Park,Jae-Wook Choi,박석용,Ahn-Heum Eom 한국균학회 2023 韓國菌學會誌 Vol.51 No.3

In the present study, we isolated endophytic fungi from different tissues of two plants,Pseudosasa japonica and Quercus serrata from Taean-gun, Korea. Morphologicalcharacteristics of the isolated fungal strains were observed. We identified the fungi basedon the phylogenetic analysis using DNA sequences from the region of internal transcribedspacer, large subunit rDNA, β-tubulin, and translation elongation factor 1-α DNA. Threeendophytic fungi that were not previously recorded in Korea, namely Geomyces asperulatus,Leptoxyphium fumago, and Tubakia oblongispora were identified. Here, we describe themorphological characteristics of these unrecorded fungi and present the results of thephylogenetic analysis.

ORiginal Article : The Risk Factors for Bleeding of Fundal Varices in Patients with Liver Cirrhosis

( Eui Ju Park ),( Jae Young Jang ),( Ji Eun Lee ),( Soung Won Jeong ),( Sae Hwan Lee ),( Sang Gyune Kim ),( Sang Woo Cha ),( Young Seok Kim ),( Young Deok Cho ),( Joo Young Cho ),( Hong Soo Kim ),( Bo The Editorial Office of Gut and Liver 2013 Gut and Liver Vol.7 No.6

Background/Aims: The relationship between portal he-modynamics and fundal varices has not been well docu-mented. The purpose of this study was to understand the pathophysiology of fundal varices and to investigate bleeding risk factors related to the presence of spontaneous portosys-temic shunts, and to examine the hepatic venous pressure gradient (HVPG) between fundal varices and other varices. Methods: In total, 85 patients with cirrhosis who underwent HVPG and gastroscopic examination between July 2009 and March 2011 were included in this study. The interrelation-ship between HVPG and the types of varices or the presence of spontaneous portosystemic shunts was studied. Results: There was no significant difference in the HVPG between fundal varices (n=12) and esophageal varices and gastro-esophageal varices type 1 (GOV1) groups (n=73) (17.1±7.7 mm Hg vs 19.7±5.3 mm Hg). Additionally, there was no sig-nificant difference in the HVPG between varices with spon-taneous portosystemic shunts (n=28) and varices without these shunts (n=57) (18.3±5.8 mm Hg vs 17.0±8.1 mm Hg). Spontaneous portosystemic shunts increased in fundal vari-ces compared with esophageal varices and GOV1 (8/12 pa-tients [66.7%] vs 20/73 patients [27.4%]; p=0.016). Conclu-sions: Fundal varices had a high prevalence of spontaneous portosystemic shunts compared with other varices. However, the portal pressure in fundal varices was not different from the pressure in esophageal varices and GOV1. (Gut Liver 2013;7:704-711)

New thyristor-based hybrid DC circuit breaker with reverse injection of resonant current

Cha, Jee‑Yoon,Lee, Eui‑Jae,Han, Byeol,Lee, Kyo‑Beum,Han, Byung‑Moon The Korean Institute of Power Electronics 2022 JOURNAL OF POWER ELECTRONICS Vol.22 No.11

This paper proposes a thyristor-based hybrid DC circuit breaker (HDCCB), which is composed of a fast mechanical circuit breaker and an LC resonant circuit with thyristor switches. The proposed HDCCB provides a zero-crossing point for fault current by injecting a resonant current in the reverse direction of the fault current. The proposed HDCCB has a low conduction loss in normal operation using a mechanical circuit breaker, and it offers the advantages of low cost, high reliability, and large capacity using thyristor switches instead of IGBT switches. It also has the design flexibility to adjust the magnitude of the injection current depending on the fault current level. A bidirectional HDCCB is also proposed by adding several components to the developed unidirectional HDCCB, which can offer compact system size and low system cost. Design considerations of the proposed structure are discussed. The validity of the proposed HDCCB is verified by simulation and experimental results.

The Dynamical Threshold Adjusting Method Using The Fuzzy Logic Operator

Jae Hyun Cho,Myung Ho Kang,Eui Young Cha 대한전자공학회 1996 ITC-CSCC :International Technical Conference on Ci Vol.- No.-

깊이 세분화 기법을 이용한 움직이는 사람 영역 검출

여재윤(Jae-Yun Yeo),이상걸(Sang-Geol Lee),김철기(Cheol-Ki Kim),차의영(Eui-Young Cha) 한국컴퓨터정보학회 2012 한국컴퓨터정보학회 학술발표논문집 Vol.20 No.2

본 논문에서는 인체의 골격 위치와 깊이 정보를 사용하여 주위 환경에 강건한 특성을 지니는 움직이는 사람 영역 검출 방법을 제안한다. 먼저 영상 내에서 인체의 골격 위치를 검출한 다음 인체 골격의 중심이 될 수 있는 지점에 대해 인체의 평균적 깊이 범위 내에서 깊이 세분화를 수행한다. 그리고 깊이 세분화를 통하여 검출된 사람 영역의 후보군에 대해 윤곽선 기반의 움직임 검출기법을 사용하여 후보군 내에서 움직이는 사람에 해당하는 특징점을 검출한다. 마지막으로 잡음 제거 및 움직이는 사람에 해당하는 영역 검출을 위하여 개선된 깊이 세분화 과정을 수행한다.

이단유로 축전식 탈염공정의 하수고도처리 적용가능성 평가

차재환(Jae Hwan Cha),신경숙(Kyung Sook Shin),이정철(Jung Chul Lee),박승국(Seung Kook Park),박남수(Nam Su Park),송의열(Eui Yeol Song) 大韓環境工學會 2014 대한환경공학회지 Vol.36 No.4

본 연구는 물 재이용을 위한 하수고도처리공정으로서 이단유로 축전식 탈염공정의 적용 가능성을 평가하였다. 일반적으로 축전식 탈염장치는 전극이 병렬식으로 단순 적층되어 있는 반면에 이단유로 축전식 탈염장치는 분리판에 의해 모듈이 2개의 단으로 구분되어 1단과 2단이 수리학적 직렬연결이 되도록 고안되었다. 동일한 유입조건에서 전산유체역학 분석결과 이단유로 모듈이 단일유로 모듈에 비해 스페이서에서 보다 균등하고 빠른 유속분포를 보였고, 사영역도 상대적으로 작게 나타났다. 하지만 입구와 출구 간 압력강하는 단일유로 모듈이 0.4 bar인 반면 이단유로 모듈은 0.7 bar로 높게 나타났다. NaCl용액을 원수로 사용한 단위셀 테스트 결과, 단일유로와 이단유로의 최대 탈염효율은 유입유량 10 mL/min/cell pair에서 각각 88%, 91%이었다. 유량증가에 따른 단위셀 압력강하의 증가는 이단유로가 단일유로보다 높게 나타났으며, 유입유량 70mL/min/cell pair에서 이단유로의 압력강하는 1.67 bar로 단일유로의 압력강하보다 2배 높게 나타났다. 이단유로의 압력강하를 완화시키기 위하여 전극 간 간격을 100에서 200 μm으로 늘린 결과, 탈염효율은 유사하게 유지하면서 압력강하는 최대 0.87 bar로 낮게 유지할 수 있었다. Proto-type 축전식 탈염장치에 하수처리수를 주입하여 연속 운전한 결과, TDS 제거효율은 평균 78%로 나타났으며, 특정이온의 경우, NH₄+-N, NO₃--N 및 PO₄ 3--P 제거효율이 각각 50%, 93% 및 50%로 나타나 질소제거 관점에서 CDI기술의 하수고도처리공정 활용가능성이 높을 것으로 판단된다. This study demonstrates a double-path CDI as an alternative of advanced wastewater treatment process. While the CDI typically consists of many pairs of electrodes connected in parallel, the new double-path CDI is designed to have series flow path by dividing the module into two stages. The CFD model showed that the double-path had uniform flow distribution with higher velocity and less dead zone compared with the single-path. However, the double-path was predicted to have higher pressure drop (0.7 bar) compared the single-path (0.4 bar). From the unit cell test, the highest TDS removal efficiencies of single- and double-path were up to 88% and 91%, respectively. The rate of increase in pressure drop with an increase of flow rate was higher in double-path than single-path. At 70 mL/min of flow rate, the pressure drop of double-path was 1.67 bar, which was two times higher than single-path. When the electrode spacing was increased from 100 to 200 μm, the pressure drop of double path decreased from 1.67 to 0.87 bar, while there was little difference in TDS removal. When proto type double-path CDI was operated using sewage water, TDS, NH₄ +-N, NO₃ --N and PO₄ 3--P removal efficiencies were up to 78%, 50%, 93% and 50%, respectively.

토종 부모계통 종계 간 교배 실용계의 경제능력 검정

차재범(Jae-Beom Cha),홍의철(Eui-Chul Hong),김상호(Sang-Ho Kim),김종대(Chong-Dae Kim),허강녕(Kang-Nyeong Heo),추효준(Hyo-Jun Choo),오기석(Ki-Seok Oh),강보석(Bo-Seok Kang) 한국가금학회 2016 韓國家禽學會誌 Vol.43 No.4

본 시험은 국내 실정에 맞는 토종 실용계 생산을 위한 13교배종의 능력을 조사하기 위하여 수행하였다. 공시동물은 국내에서 사육되고 있는 토종 순종계를 이용하여 생산된 종계 부계통 2종(A, B)과 모계종 6종(1, 2, 3, 4, 5, 6)을 교배하여 생산된 12종(1A, 1B, 2A, 2B, 3A, 3B, 4A, 4B, 5A, 5B, 6A, 6B)과 일반 토종닭 교배종(‘한협3호’, HH)의 총 13종, 종당 100수씩 총 1,300수를 암수 합사하여 공시하였다. 시험사료는 0∼7일령까지는 육계초이사료(CP 22.0% ME 3,150 kcal/kg), 8일령부터 체중 800 g까지는 육계전기사료(CP 19.0% ME 3,150 kcal/kg), 체중 800 g∼12주령까지는 육계후기사료(CP 18.0% ME 3,150 kcal/kg)를 급여하였다. 주요 조사항목은 수정률과 부화율, 체중, 균일도, 사료요구율, 생산지수 그리고 경제성 등이었다. 수정률은 1B종이 가장 높았으며, 6B와 HH종이 낮게 나타났다. 수정란 대비 부화율은 87.5∼89%, 입란대비 부화율은 76.9∼92.3%로 나타났으며, 4B와 6B종이 낮은 수치를 보였다. HH종의 주령별 체중은 다른 교배종에 비해 낮게 나타났다. 체중의 CV가는 12.5∼18.2%로 13종 모두 12%보다 높게 나타났으며, 특히 2B, 3B, 5B, HH종에서 높게 나타났다. 누적 사료요구율은 6, 8, 10, 12주령에 각각 1.7 ∼2.3, 2.1∼2.5, 2.4∼2.7 및 2.7∼3.2로 나타났다. 주령별 생산지수는 2A, 3A, HH종에서 낮게 나타났으며, 주령이 경과함에 따라 생산지수는 감소하였다. kg 증체당 사료비는 육계초기 1,069∼1,255원, 육계전기 1,100∼1,263원, 육계후기 1.468∼1,696원으로 나타났다. 본 연구의 결과를 분석한 결과, 기존 토종닭보다 생산성이 우수하고 경제성을 갖춘 새로운 토종 실용계 선발이 가능하다는 결론을 도출하였다. This work was carried out to investigate the ability of thirteen crossbreds for production of commercial chicken (CC) of Korean native chicken to be cut out for Korean circumstance. Total of one thousand three hundred of crossbreds were divided thirteen groups (100 birds/group) that were twelve groups (1A, 1B, 2A, 2B, 3A, 3B, 4A, 4B, 5A, 5B, 6A, 6B) to crossbreed with two sires (A, B) and six dams (1, 2, 3, 4, 5, 6) of parent stock (PS) of Korean native chicken and one group (HH) as commercial Korean native chicken. Experimental diets were starter diet (0∼7d; CP 22.0% ME 3,150 kcal/kg), early diet (8d∼Wt 800 g; CP 19.0% ME 3,150 kcal/kg) and finished diet (Wt 800 g∼12 wk; CP 18.0% ME 3,150 kcal/kg). Fertility of 1B group was higher than other groups. Hatchability per fertilization eggs and hatchability per setting eggs were 87.5∼89% and 87.9∼96.3%, respectively, and those of 4B and 6B groups were lower compared to other groups. Weekly body weight of HH group was lower than other groups. CV values of body weight of thirteen groups were showed over 12% as 12.5∼18.2%, and especially, those of 2B, 3B, 5B, HH groups were lower compared to other groups. Weekly feed conversion ratios (FCRs) of 13 crossbreds were 1.7∼2.3, 2.1∼2.5, 2.4∼2.7 and 2.7∼3.2 at the age of 6, 8, 10 and 12 weeks, respectively. Weekly production indexes of 2A, 3A and HH groups were the lower than other groups and those of thirteen groups decreased following weeks. Feed costs per body weight gain (kg) were 1,069∼1,255 won, 1,100∼1,263 won, and 1.468∼1,696 won at starter, earlier and finished period of broilers. These results leaded down to conclusion to be possible selection of new Korean native commercial chicken that have the excellent performance and economics.