Genome-wide investigation of a Korean synthetic breed, Woori-Heukdon using the Illumina PorcineSNP60K BeadChip

Yong‑Min Kim,Ha‑Seung Seong,Jung‑Jae Lee,Da‑Hye Son,Jin‑Su Kim,Soo‑Jin Sa,Young‑Sin Kim,Tae‑Jeong Choi,Kyu‑Ho Cho,Joon‑Ki Hong,Jung‑Woo Choi,Eun‑Seok Cho 한국유전학회 2020 Genes & Genomics Vol.42 No.12

Background: Woori-Heukdon (KWH) is a Korean synthetic pig breed generated using Chookjin-Duroc (KCD), Chookjin-Chamdon (KCC), and their crossbreds. Currently, there is a severe lack of studies investigating the Korean breed populations including wild boars (KWB) throughout the genome. Objective: This study was performed to investigate the genetic characteristics of Korean pig populations at the genome-wide level. Methods: Using the SNP dataset derived from genotyped and downloaded datasets using the Illumina PorcineSNP60K BeadChip, we compared the genomes of 532 individuals derived from 23 pig breeds to assess the genetic diversity, inbreeding coefficient, genetic differentiation, and population structure. Results: KWB showed the lowest average expected heterozygosity (HE = 0.1904), while KWH showed the highest genetic diversity (HE = 0.02859) among Korean populations. We verified that the genetic composition of KWH, showing USD of 74.8% and KCC of 25.2% in ADMIXTURE analysis. In population structure analyses, KCC was consistently shown to be separated from other pig populations. In addition, we observed gene flow from Western pigs to a part of Chinese populations. Conclusion: This study showed that Korean native pigs, KCC have genetic differences in comparison with Chinese and Western pigs; despite some historical records and recent genetic studies, we could not find any clear evidence that KCC was significantly influenced by Chinese or Western breeds in this study. We also verified the theoretical genomic composition of KWH at the molecular level in structure analyses. To our knowledge, this is the first genomic study to investigate the genomic characteristics of KWH and KCC.

한국인의 사회 재적응률 척도에 관한 조사

조주연,민성삼,이영진,조동영,이혜리,윤방부 대한가정의학회 1985 Korean Journal of Family Medicine Vol.6 No.12

분당 신도시 개발 이후 용인시의 공간구조 변화에 대한 연구

조지혜,김영욱,박영기 대한건축학회 2003 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.23 No.1

The purpose of this study was to analyze quantitatively the transformation of spatial structure in the near city of a newtown after the development of it The result is that the development of a newtown had an effect on the trans formation of spatial structure in the near city. The analysis shows that Yongin city's spatial configuration with near cities became strong. And its northwest was more integrated forward Bundang new city than old urban core of it. As a result, it shows a decline of the old urban core.

서울시 대기오염과 일별 사망의 상관성에 관한 시계열적 연구 (1998~2001년)

조용성,이종태,김윤신,홍승철,김호,하은희,박혜숙,이보은 한국대기환경학회 2003 한국대기환경학회지 Vol.19 No.6

This study was performed to examine the relationship between air pollution exposure and mortality in Seoul for the years of 1998~2001I. Daily counts of death were analyzed by general additive Poisson model, with adjustment for effects of seasonal trend, air temperature, humidity, and day of the week as confounders in a nonparametric approach. Daily death counts were associated with CO (current day), 0₃(current day), PM_(10) (current day), NO₂, (1 day before), SO₂, (1 day before). Increase of 41.711㎍/㎥ (interquartile range) in PM_(10) was associated with 1.3% (95% CI = 0.7~1.9%) increase in the daily number of death. 0₃concentrations resulted in an increased risk of 1.3% for 23.86 ppb in all-aged mortality [RR = 1.013 (1.004-1.023)]. This effect was greater in children (less than 15 aged) and elderly (more than 65 aged). After ozone level exceeds 25 ppb, the dose-response relationship between mortality and ozone was almost linear. We concluded that Seoul had 1~5% increase in mortality in association with IQR (interquartile range) in air pollutants. Daily variations in air pollution within the range currently occurring in Seoul might have an adverse effect on daily mortality. These findings also support the hypothesis that air pollution, at levels below the current ambient air quality standards of Korea, is harmful to sensitive subjects, such as children or elderly.

만성신부전증 환자의 객담에서 분리한 Staphylococcus aureus, Small Colony Variants 1예

이혜수,조용곤,김달식,최삼임 대한임상미샐물학회 2003 Annals of clinical microbiology Vol.6 No.2

회맹장 및 대장을 침범한 장 결핵에 있어서 바륨을 이용한 방사선 이중조영촬영 소견에 대한 연구

진용현,전혜정,이용근,강철호,조성범,김윤환,박철민,차상훈,김일영,이혜경 건국대학교 의과학연구소 2002 건국의과학학술지 Vol.12 No.-

To determine the characteristic double contrast barium enema findings of ileo-cecal and colonic tuberculosis and to make differential diagnosis from other bowel inflammatory disease. Sixty-seven patients confirmed as ileo-cecal and/or colonic-tuberculosis in six hospitals were evaluated retrospectively. The diagnosis was proven by endoscopic biopsy, surgery, and clinical follow up. Nine of 67 patients were excluded because radiologic findings were normal though endocopic biopsy examinations resulted in tuberculosis. Histopathological diagnosis were madein 58 cases as follows: endoscopic biopsy(n=29), operation(n=12), follow up examination(n=14), tissue culture(n=1), laparoscopic biopsy(n=2). There were 30 men and 28 females. The mean age were 36 yrs(range:16-67), 34 patients were in the second and third decade. The distribution of lesions, morphological characteristics, mucosal changes, ulcer shape and depth, associated with other tuberculous lesion, multiplicity were retrospectively analyzed in the findngs of double contrast barim enema. The commonest clinical symptoms were abdominal pain(n=35, 60%) and diarrhea(n=18, 31%). The chest radiography revealed association of pulmonary tuberculosis in 39 cases. Tuberculous lesions of other organs except chest were present in five cases. Double contrast barium enema findings showed a thickening and irregularity of intestinal mucosal fold(n=29, 50%), bowel contraction and/or shortening(n=26, 45%). There were small, nodular, superficial ulcers in the case of ulcer depth less than 2 mm in the depth. As many as 39 cases(67%) in this series had two or more lesions in the intestinal tuberculosis. The involved segment revealed symmetrical feature(n=21, 36%). Inflammatory pseudopolypoid lesions were noted in 30 cases(52%). The ileo-cecal region was involved in 38 cases(66%). The ascending colon(n=25, 43%) was the second commonest site with or without contiguous cecal involvement. Lesions in terminal ileum, ileo-cecal valve, transverse colon, descending colon and appendix were seen in 19, 18, 18, 12 and seven cases, respectively. Among 18 cases of ileo-cecal valve lesions, there were incompetency in nine cases(16%) and wide gapping in four cases(7%). In 14 cases, tuberculous enterocolitis was diagnosed by clinical follow-up and double contrast barium enema study. 12 out of 14 cases, the findings of double contrast barium enema of tuberculous enterocolitis were normalized after anti-tuberculous medication. However, two cases showed more aggravated state than before due to inappropriate treatment. Double contrast barium enema plays an important role in the diagnosis of tuberculous enterocolitis, determines the type, site and extent of the involvement and helps in treatment guide by using sequential studies.

전전뇌(Holoprosencephaly) 4례 : 임상 및 부검소견 The Clinical and Autopsy Findings

박용원,김성환,조혜제 인제대학교 1986 仁濟醫學 Vol.7 No.2

We experienced 4 cases of holoprosencephaly diagnosed by clinical and autopsy findings. First case showed normal external feature with alober type of holoprosencephaly, second case showing nearly normal external feature with semilobar type of holoprosencephaly, third case showing cyclopia with alobar type of holoprosencephaly, and fourth case showing cebocephaly with lobar type of holoprosencephaly. Holoprosencephaly seems to be a malformation of broad spectrum having various facial dysmorphia and CNS anomalies, as presented by our cases.

포도 추출물들의 자유 라디칼 소거 작용 및 마우스 대식세포주의 염증 발현 매개 인자들에 대한 생성 억제 효과

민혜영,박은정,이상국,조용진 梨花女子大學校 藥學硏究所 2003 藥學硏究論文集 Vol.- No.12

국내에서 생산된 거봉 및 캠벨 포도의 씨, 줄기 및 껍질추출물에 대하여 항산화 작용, 염증 관련 인자 생성에 미치는 활성 및 암세포 성장에 대한 영향 등을 resveratrol과 비교하여 평가하였다. 그 결과 포도 추출물 중 거봉줄기, 캠벨줄기, 캠벨씨 및 거봉씨 추출물들이 항산화 능력을 나타내었고 그 중 거봉씨 추출물은 vitamin C와 효력이 유사하게 나타나 항산화 효능이 우수함을 알 수 있었다. 또한 마우스 대식세포주인 RAW 264.7 cell을 이용하여 포도 추출물들의 LPS 처리에 의한 PGE₂ 및 NO 생성을 저해 여부를 확인한 결과, 거봉줄기, 거봉씨, 및 캠벨씨 추출물이 50 ㎍/mL에서 PGE₂ 및 NO 생성을 50% 가량 저해하는 효능을 나타내었다. 또한 사람 폐암 및 대장암 세포주를 이용하여 포도 추출물들이 암세포 성장 저해 효과를 나타내는지를 확인하였는데 거봉줄기 및 씨 추출물 50㎍/mL에서 30% 정도의 암세포 성장 저해 작용을 나타내었다. Antioxidant and anti-inflammatory potentials of various grape extracts were evaluated. Extracts from Kyho seed, Kyho stem, and Campbell seed showed potent l,l-diphenyl-2-picrylhydrazyl (DPPH.) free radical scavenging activities compared to resveratrol (IC_(50) 16.9, 21.5, 21.9, 34.6 ug/mL, respectively), among which, antioxidant effect of Kyho seed extract were similar to that of vitamin C (IC_(50)=12.2㎍/mL). These extracts also exhibited inhibitory activities on lipopolysaccharide (LPS)-induced prostaglandin E₂ production and nitrite formation in mouse macrophage RAW 264.7 cells at 50㎍/mL. Kyho stem and seed extracts showed growth inhibitory activities in human lung and colon cancer cells. These results suggest the potential roles of grape extracts as antioxidants and anti-inflammatory agents.

다운 증후군과 동반된 그레이브스병 1예

노혜정,신진호,김하영,김용성,백승훈,박병현,조정구 圓光大學校 醫科學硏究所 2005 圓光醫科學 Vol.20 No.1

Whole-genome resequencing analysis of 20 Micro-pigs

Da‑Hye Son,Nam‑Hyun Hwang,Won‑Hyong Chung,Ha‑Seung Seong,Hyungbum Lim,Eun‑Seok Cho,Jung‑Woo Choi,Kyung‑Soo Kang,Yong‑Min Kim 한국유전학회 2020 Genes & Genomics Vol.42 No.3

Background Miniature pigs have been increasingly used as mammalian model animals for biomedical research because of their similarity to human beings in terms of their metabolic features and proportional organ sizes. However, despite their importance, there is a severe lack of genome-wide studies on miniature pigs. Objective In this study, we performed whole-genome sequencing analysis of 20 Micro-pigs obtained from Medi Kinetics to elucidate their genomic characteristics. Results Approximately 595 gigabase pairs (Gb) of sequence reads were generated to be mapped to the swine reference genome assembly (Sus scrofa 10.2); on average, the sequence reads covered 99.15% of the reference genome at an average of 9.6-fold coverage. We detected a total of 19,518,548 SNPs, of which 8.7% were found to be novel. With further annotation of all of the SNPs, we retrieved 144,507 nonsynonymous SNPs (nsSNPs); of these, 5968 were found in all 20 individuals used in this study. SIFT prediction for these SNPs identified that 812 nsSNPs in 402 genes were deleterious. Among these 402 genes, we identified some genes that could potentially affect traits of interest in Micro-pigs, such as RHEB and FRAS1. Furthermore, we performed runs of homozygosity analysis to locate potential selection signatures in the genome, detecting several loci that might be involved in phenotypic characteristics in Micro-pigs, such as MSTN, GDF5, and GDF11. Conclusion In this study, we identified numerous nsSNPs that could be used as candidate genetic markers with involvement in traits of interest. Furthermore, we detected putative selection footprints that might be associated with recent selection applied to miniature pigs.