한국여성에서 자궁내막증의 발생위험도와 Estrogen Receptor-α 유전자 다형성과의 관련성에 관한 연구

이사라,허성은,문혜성,김형래,정혜원 이화여자대학교 의과대학 2003 EMJ (Ewha medical journal) Vol.26 No.2

Objectives: To investigate whether polymorphism of gene encoding estrogen receptor-a is asso-ciated with the risk of endometriosis in Korean women. Material and Methods :We investigated 136 patients with histopathologically confirmed endo-metriosis rAFS stage III/IV and 251 control group women who were surgically proven to have no endometriosis. Polymerase chain reaction(PCR) and restriction fragment length polymorphism (RFLP) of PCR products were done to determine each participant's estrogen receptor-a genotype. Results : The distridution according to PvuII genetic polymorphism of estrogen receptor-a were as follows. PP, Pp and pp were 14.7%(20 women), 39.0%(53 women) and 46.3%(63 women) in the study group and 13.9%(35 women), 38.6%(98 women) and 47.4%(119 women) in the con-trol group, respectively. There was no significant difference between the study group and the control group. Conclusion : The results suggest that estrogen receptor-a genetic polymorphism may not be associated with the development of endometriosis in Korean women. 목적: 자궁내막증은 에스트로겐에 의존적인 질환이므로 에스트로겐의 합성, 대사 및 작용에 관여하는 유전자의 다형성이 자궁내막증의 발생기전에 중요한 역할을 할 수 있다. 이에 본 연구에서는 한국인 여성에서 에스트로겐 수용체-a의 유전자 다형성이 자궁내막증의 발생 위험도를 증가시키는 지에 대해 연구하고자 하였다. 방법: 1996년 9월부터 2003년 8월가지 본원 산부인과에서 수술을 통해 병리조직학적으로 자궁내막증 III기와 IV기를 확인한 한국인 여성 136명을 대상으로 하였다. 대조군은 자궁내막증 환자군과 연령이 비슷한 만삭 산모에서 제왕절개술을 시행하거나 양성 난소낭종으로 수술을 시행 하였을 때, 자궁내막증이 없음을 확인한 여성 251명 으로 하였다. 결과: ER-a 유전자의 PvuII 다형성의 분포는 자궁내막증 환자군에서 PP군이 20명(14.7%), Pp군이 53명(39.0%), pp군이 63명(46.3%) 이었고 대조군에서의 분포는 각각 35명(13.9%), 97명(38.7%), 119명(47.4%)으로 나타났으며 자궁내막증 환자군과 대조군 사이의 유의한 차이는 없었다. Pp, pp형을 가지는 경우가 자궁 내막증 환자의 85.3%(116명), 대조군의 86.1%(216명)로 나타났으며, 이 경우 자궁내막증이 발생할 odds ratio가 0.904(95% CI, 0.519~1.702)로 통계적으로 유의한 차이가 없었다. 결론: 이상의 결과로 볼 때, 한국인 여성에서 자궁내막증의 발생위험과 에스트로겐 수용체 a 유전자 다형성간에 연관성은 없는 것으로 나타났다.

F9 기형암종 세포의 분화에 따른 small GTP-binding protein변화

박혜성,이준승,김규원,허규정 이화여자대학교 생명과학연구소 1994 생명과학연구논문집 Vol.5 No.-

세포분화에 따른 Small GTP-binding protein의 역할을 밝히기 위하여 Retinoic acid(RA)와 dibutyryl cyclic AMP(dbcAMP)로 분화를 유도한 F9 기형암종세포의 형태적인 변화와 함께 Small GTP-binding protein의 분포를 조사하였다. RA와 dbcAMP를 처리한 세포는 분화유도 5일경(초기 분화 단계)에 분명한 세포의 경계를 보이기 시작하여 7일경(분화 후기 단계)에는 거의 모든 세포가 둥근 분화된 형태로 전환되었다. 이 분화과정 동안 세포막에는 많은 microvilli와 lamellopodia 같은 구조물이 나타났다. 아울러 초기 분화 단계에 많은 량의 laminin이 발현되었으며 분화 후기에 microtubule의 재분포가 관찰되었다. 세종류의 Small GTP-binding protein(25, 23, 21 KD)이 F9 세포의 막성분과 세포질에서 관찰되었으며 분화가 진행됨에 따라서 세단백질 모두 증가되는 양상을 보였다. 이러한 결과는 Small GTP-binding protein이 F9 세포의 분화에 특별한 기능을 가지고 있음을 시사해 주고 있다. To address possible roles of the small GTP-binding protein in differentiation of F9 teratocarcinoma stem cells, the distribution and amount of these proteins together with morphological changes of the cells were sstudied during the differentiation of F9 cells induced by retinoic acid and dibutyryl cyclic AMP (dbcAMP). When monolayer cultures of F9 cells were induced to differentiate with retinoic acid and dbcAMP, the cells started to show distinctive cell boundaries in about 5 days (early differentiation stage) and reached fully differentiated shapes in about 7 days (late differentiation stage). During this differentiation process, membranes of the cells developed a large number of microville and lamellopodia. In addition, expression of laminin increased maximally in early stage, then microtubule rearrangement followed in late differentiation stage. Three classes of small GTP-binding proteins with sizes of 25, 23 and 21 kD were found in both microsomes and cytosols of F9 cells. These proteins increased along with progress of the differentiation. These results suggest that these small GTP-binding proteins likely have specific roles in the differentiation of F9 cells.

F9 기형암종 세포의 분화에 따른 small GTP-binding protein변화

박혜성,이준승,김규원,허규정 부산대학교 유전공학연구소 1994 분자생물학 연구보 Vol.10 No.-

세포분화에 따른 Small GTP-binding protein의 역할을 밝히기 위하여 Retinoic acid(RA)와 dibutyryl cyclic AMP(dbc AMP) 로 분화를 유도한 F9 기형암종세포의 형태적인 변화와 함께Small GTP-binding protein의 분포를 조사하였다. RA와 dbc AMP를 처리한 세포는 분화유도 5일경(초기 분화 단계)에 분명한 세포의 경계를 보이기 시작하여 7일경(분화 후기 단계)에는 거의 모든 세포가 둥근 분화된 형태로 전환되었다. 이 분화과정 동안 세포막에는 많은 microville와 lamellopodia 같은 구조물이 나타났다. 아울러 초기 분화 단계에 많은 량의 laminin이 발현되었으며 분화 후기에 microtuble 의 재분포가 관찰되었다. 세종류의Small GTP-binding protein(25,23,21 KD )이 F9 세포의 막성분과 세포질에서 관찰되었으며 분화가 진행됨에 따라서 세단백질 모두 중가되는 양상을 보였다. 이러한 결과는Small GTP-binding protein이 F9세포의 분화에 특별한 기능을 가지고 있음을 시사해주고 있다. To adress possible roles of the small GTP-binding proteins in differentation of F9 teratocarcinoma stem cells, the distribution anf amount fo these proteins together with morphological changes of the cells were studied during the differentiation of F9 cells induced by retionic acid and dibutyryl cyclic AMP(dbc AMP). When monolayer cultures of F9 cells were induced to differentiate with retionic acid dbc AMP, the cells started to show distinctive cell boundaries in about 5 days(early differentiation stge) and reached fully differentiated shaper in about 7 days(late differentiation stage). During this differentiation process, membranes of the cells developed a large number of microville and lamellopodia. In addition, expression of laminin increased maximally in early stage, then microtuble rearrangement followed in late differentiation stage. Three classes of small GTP-binding proteins with sizes of 25,23 and 21 kD were fiund in both microsomes and cytosols of F9 cells. These proteins increased along with progress of the differentiation. These results suggest that these small GTPbinding proteins likely have specific roles in the differentiation of F9 cells.

Prognostic significance of cytoreductive surgery in primary peritoneal carcinoma

허혜원 ( Hye Won Hur ) 대한산부인과학회 2006 대한산부인과학회 학술대회 Vol.92 No.-

C-reactive protein concentrations and differential distributions of Leukocytes in preeclampsia(초)

허혜원 ( Hye Won Hur ),( Ga Hyun Son ),( Seung Chul Lim ),( Dong Wook Kwak ),( Ja Young Kwon ),( Yong Won Park ) 대한산부인과학회 2009 대한산부인과학회 학술대회 Vol.95 No.-

Simultaneous quantification of 60 elements associated with dried red peppers by ICP-OES and ICP-MS for routine analysis

Suel Hye Hur,Jae-Min An,Ji-Hye Lee,Yong-Sik Youn,Hee-Chang Shin,Bo Rim Shin,Ji won Kim,Ho Jin Kim 한국분석과학회 2022 학술대회논문집 Vol.2022 No.5

Poster Session : PS 1506 ; Rheumatology : Effect of Oral Prostacyclin Analogue on Serum TNF-Alpha Level in Patients with Rheumatoid Arthritis

( Hye Won Kim ),( Jin Wuk Hur ) 대한내과학회 2014 대한내과학회 추계학술발표논문집 Vol.2014 No.1

Background: Prostacyclin that binds to prostacyclin receptor on platelets and vascular smooth muscle cells increase intracellular c-AMP and block infiux of calcium, resulting in antiplatelet and vasodilatory effect. As such, prostacyclin analogue is often attempted to patients who are accompanied by peripheral vasculopathy. TNF-alpha is a representative in. ammatory marker in rheumatoid arthritis (RA), known to be integral target to treat. Assuming that prostacyclin may show anti-infiammatory effect, we aim to investigate serum TNF-alpha level before/after prostacyclin analogue treatment in patient with RA. Methods: Patients with RA suffering from symptom of vasculopathy (age > 20 years, diagnosed as RA at least 3 months earlier, on stable disease modifying anti-rheumatic drug therapy) were included. Participants were given 0.02mg three times a day of beraprost sodium, an oral prostacyclin analogue. CBC, ESR, CRP, serum TNF-alpha level were measured at baseline and 4, 12 weeks after treatment. Interviews on peripheral symptoms such as tingling sense, coldness of hand and Raynaud`s phenomenon were processed at baseline and 12 weeks after treatment. Results: Thirty-two patents were enrolled and 25 patients (male (n = 8), female (n = 17), mean disease duration 3.7 years) completed the study for 12 weeks. Most patients were on 3 or more DMARDs. Patients with ESR = 40mm/hr or with CRP = 2.0 mg/dL showed a declining tendency of serum TNF-alpha level at 4 weeks and at 12 weeks after prostacyclin analogue treatment, though it did not reached statistical signifi- cance (p=0.367, p=0.227) probably due to lack of numbers of participants. Conclusions: TNF-alpha is an integral pro-in. ammatory cytokine, against which targeted treatment for RA are fiourishing. We showed serum TNF-alpha level before and after treatment of prostacyclin analogue. Our results suggest anti-in. ammatory effect of prostacyclin that may have a role in treatment of RA.

Clinical and neurocognitive profiles of subjects at high risk for psychosis with and without obsessive-compulsive symptoms

Hur, Ji-Won,Shin, Na Young,Jang, Joon Hwan,Shim, Geumsook,Park, Hye Yoon,Hwang, Jae Yeon,Kim, Sung Nyun,Yoo, Jung Hyun,Hong, Kyung Sue,Kwon, Jun Soo SAGE Publications 2012 Australian and New Zealand journal of psychiatry Vol.46 No.2

<P>Obsessive-compulsive symptoms (OCS), which are common in psychotic-spectrum illnesses, are of clinical interest because of their association with poor prognosis or cognitive dysfunction. However, few studies on the clinical and neurocognitive implications of OCS in individuals at ultra-high risk for psychosis (UHR) have been conducted.</P>

P010 Ellagic acid protects human dermal papilla against UVA-induced oxidative stress

( Hye In Cheon ),( Min Seok Hur ),( Byung Gon Choi ),( Song Hee Han ),( Min Jung Kim ),( Hae Jeong Youn ),( Soo Young Kim ),( Nam Kyung Roh ),( Yang Won Lee ),( Yong Beom Choe ),( Kyu Joong Ahn ) 대한피부과학회 2016 대한피부과학회 학술발표대회집 Vol.68 No.2

<div style="display:none">fiogf49gjkf0d</div><div style="display:none">fiogf49gjkf0d</div><div style="display:none">fiogf49gjkf0d</div><div style="display:none">fiogf49gjkf0d</div><div style="display:none">fiogf49gjkf0d</div><div style="display:none">fiogf49gjkf0d</div><div style="display:none">fiogf49gjkf0d</div> Background: One of solar radiation components, ultraviolet A (UVA), has deeply penetrating capacity and generates reactive oxygen species (ROS) in the skin. These characteristics arouse oxidative stress in dermis, such as photoproducts, senescence, cell death, inflammation, and alterative gene expression. Objectives: This study aimed at investigating the antioxidant and anti-inflammatory effects of ellagic acid on UVA-irradiated human dermal papilla cells (HDPCs). Methods: The cell viability of HDPCs were analyzed using the water-soluble tetrazolium salt (WST-1) assay. 2,7-dichlorofluorescin diacetate (DCFH-DA) was used in evaluating intracellular ROS scavenging activity. All the mRNA expression levels were carried out via quantitative real-time polymerase chain reaction (qRT-PCR). Cellular senescence was evaluated using senescence-associated β -galactosidase (SA-β-gal) staining kit. Results: In irradiated HDPCs, ellagic acid showed protective effects on UVA-induced cytotoxicity. Further, ellagic acid represented ROS scavenging activity and regulating antioxidant gene expression, catalase transcriptional levels under UVA-irradiated condition. It also modulated tumor necrosis factor alpha (TNF-α) and interleukin 6 (IL-6) mRNA expresison levels and cellular senescence on UVA-stimulated HDPCs. Conclusion: These results demonstrate that ellagic acid protects UVA-induced oxidative stress in HDPCs.

A case of pigmented squamous cell carcinoma on the pubic area

( Hye In Cheon ),( Ji Youn Hong ),( Jin Hee Kim ),( Joo Ran Hong ),( Min Seok Hur ),( Byung Gon Choi ),( Song Hee Han ),( Yang Won Lee ),( Yong Beom Choe ),( Kyu Joong Ahn ) 대한피부과학회 2018 대한피부과학회 학술발표대회집 Vol.70 No.1

A 62-year-old woman presented with a well-circumscribed, dark brown-to-black colored, round shaped, elevated patch with 8 mm sized in diameter on the pubic area. The size of the lesion had been enlarged from a few years ago. In histopathologic examination, acantholysis and proliferation of atypical keratinocytes were observed. The tumor cells with abundant eosinophilic cytoplasm and multiple mitotic nuclei penetrated into the upper dermis. The abundant melanin pigment was observed in epidermis diffusely. The final diagnosis was pigmented microinvasive squamous cell carcinoma. The lesion was completely removed surgically with adequate margins. Pigmented squamous cell carcinoma (SCC) is a rare variant of SCC with 0.01% to 7% prevalence in SCC. Pigmented SCC can be misdiagnosed with benign or malignant melanocytic neoplasms due to similarities of clinical features and its rarity. We report the rare case of pigmented SCC clinically suspicious for melanocytic nevus.