Rotavirus 전기영동형별을 위한 RNA 추출과정 및 조건의 비교연구

조은경,김은순,양재명,김경희,조양자 한양대학교 의과대학 1992 한양의대 학술지 Vol.12 No.2

Rotaviruses (Rv) are the major cause of severe dehydrating dearrhea among young children in Korea, where they are frequently responsible for 68% of the cases requiring hospitalization. Laboratory diagnosis of rotaviral infection in Korea depends mainly on enzyme-linked immunosorbent assay (ELISA) that employs expensive commercial diagnostic kits purchased from abroad and that shous the presence or absence of group A Rv only. PACE have however provided epidemiologic evidence to demonstrate the sxistence of different strains (both group A and atypical Rv) during diarrgeal outbreak, the appearance of new strains and desappearance of old ones from a community, shifts in the prenalence of strains, and the persistance of a single strain in newborns. For such reasons, we have felt that it is essential to raise the sensitivity and shorten the processing time for PAGE. We therfore have devised a rapid method for Rv RNA extration by directly extracting the Rv RNA from the specimens (that is, skipping freon extraction and running as ultracectrifuge) and using an urea-containing dissociation mixture to dissolve dried RNA for PAGE. We have also shorten the PAGE diagnostic results by sequential staining of the same gel with ethidium bromide and silver stain. With the use of the rapid method, it was feasible to increase the efficacy of the PAGE with reduced cost and working time. Phenol extration of stools, however, was essential in maintaining the sensitivity of the method.

영아 천식의 임상적 고찰 : Asthma prospective score에 의한 Based on Asthma Prospective Score

양은석,노영일,문경래,박영봉,박상기,변주남 조선대학교 부설 의학연구소 1999 The Medical Journal of Chosun University Vol.24 No.1

A Clinical study was made on 40 patients for infantile asthma less than 2 years old who had been admitted to the Department of Pediatrics of Chosun University Hospital from January 1997 to December 1998. We evaluated family history of allergic diseases, clinical features, serum IgE level, eosinophilia, chest radiologic findings and response to bronchodilator. The results obtained were as follows ; 1) The male to female ratio of patients studied was 4 : 1. 2) Eighteen of the 40 patients (45.0%) had a family history of allergic diseases, of which 10 patients had maternal allergic diseases. 3) Atopic dermatitis was the most common disease (37.5%) among the associated allergic diseases. 4) Thirty two of the 40 patients (80.0%) showed high serum total 19E levels. 5) Only 5 of the 20 patients who were examined by MAST showed positive reaction. 6) The frequency of chest radiologic findings observed in these patients was as follows ; hyperinflation (52.5%), increased bronchovascular marking(30.0%), no pathologic findings(25.0%), and pneumonic infiltration(25.0%). 7) In response to bronchodilator 28 cases(70.0%) were effective. 8) When we divided the 40 patients into two groups by the diagnostic criteria of Asthma prospective score (AP score), 30 patients(75.0%) were included in a compatible group. On the basis of AP score, family history of major or minor allergic dieases and the responsiveness to bronchodilator were important factors for the diagnosis of asthma.

1950년대 서울番地案內圖와 서울商界略圖에 대한 지도학 및 역사지리학적 고찰

양보경,장은미 서울시립대학교 부설 서울학연구소 2005 서울학연구 Vol.- No.24

This paper aims to analyze Seoul Address Map published in 1956 and Seoul Commercial Map published in 1955 after Korean War. Two maps are converted into a raster format and partly vectorized to explore the contents of maps in cartographic aspects and advertisement items depicted with the map in historical-geographical aspect. The Seoul Address Map map is being housed at the map library of University of Kansas, while 1955 map at the map library at Yale University. Published by a private company (Daehan Annae Sa), it is different from the previous maps published (luring the period of Japanese conquest. The Seoul Address Map inclined about 45.58˚(stdev= 0.997) for Jongro street and 44.5479 (stdev= 3.031) for Euljiro from the horizontal line while the Seoul Commercial Map has the same inclination with modern digital maps and address Map published in 2001 with north side up. The Seoul Address Map does show less pictorial description for geographic features than Seoul Commercial Map. Seoul Address Map includes 64 advertisements most of which were hospitals, and securities(banking) located in Junggu and Jongrogu. The Commercial Map includes 67 advertisements, of which are other industrial product such as furniture, food and clothes with higher variety than the other map. Maps on Korea are scattered and housed at other countries and antique bookstores need to be monitored and digitalized for further studies for realistic reconstruction of space of genre de vie.

물가자미 液化蛋白質 濃縮物의 製造 및 品質安定性

梁升澤,朴殷京 경성대학교 1987 부산수산대학 논문집 Vol.8 No.3

This study was designed to prepare liquefied roundnose flounder protein concentrate with a long self life. The sample was divided into 2 groups of whole fish and fillet. The whole roundnose flounder was chopped and mixed with same amount of water and then hydrolyzed by bromelain, papain and complex enzyme. The optimum conditions for hydrolysis of whole fish were 50℃, 4 hours by bromelain (0.5%) digestion, 55℃, 5 hours by papain (0.3%) digestion, and 55℃, 4 hours by complex enzyme (3.0%) digestion, respectively. The liquefied whole roundnose flounder protein by complex enzyme could be decolorized by the treatment with 15% activated charcoal at room temperature for 30 minutes. The decolorized hydrolysates from whole fish and fillet were concentrated to brix 25% and then dried with spray dryer in conditions of inlet temperature 185℃, outlet temperature 85℃. The products were packed in cans and checked the keeping quality such as solubility in water, soluble nitrogen, relative viscosity, emulsifying stability, color, and sensory evaluation during storage for 3 months at 5℃, 20℃, and 35℃. Both of the products had an excellent functional properties. Good quality of products was maintained until 3 months storage at 5℃ and 20℃ in both samples, but it had been lost after 15 days and 1 month at 35℃ in liquefied whole fish and fillet protein concentrates, respectively. The keeping quality of products from fillet were better than those of products from whole fish.

中國 駝山石窟 佛敎彫刻에 대한 考察

梁銀景 동국대학교 불교미술문화제연구소 1995 佛敎美術硏究 Vol.2 No.-

복강 내 체액의 Cytokine 농도를 이용한 난소암과 양성난소질환의 감별진단

양선문,송경은,서장수,조영래 대한임상병리학회 2000 대한임상병리학회지 Vol.20 No.6

모유수유 실태 및 모유수유에 영향을 미치는 요인에 대한 조사

양은석,문경래,박종 조선대학교 1995 The Medical Journal of Chosun University Vol.20 No.1

Human milk is recognized as the optimal diet for newborn and infants. The ?? of breast-feeding has increased in many countries, but in Korea is still very low. We ??veyed the present status of infant feeding methods and the reason for the failure of breast-feeding. The survey was performed by a special questionarie on 1345 cases of mothers who visited both the well baby clinic of Chosun University Hospital and local clinics in Kwangju from May to June. 1993. The results obtained are as follows: 1) The rates of breast-feeding, mixed-feeding, formula-feeding were 27.4%, 21.6%, 51.0% respectively. 2) The rate of breast-feeding was higher in babies of mothers of older age. lower eduacation level, and normal vaginal delivery. 3) Mothers recognized the significance of breast-feeding(95%) , but the rate of breast-feeding was very low. 4) Knowledge about breast-feeding was mainly from books(29.7%), parents(27%), TV and radio(20.3%), and only(9.7%) marginally from medical doctor. 5) The cause of failure of breast-feeding was the mother's concern of insufficient breast milk(48.6%), breast engorgement and pain of the nipples(16.3%), diarrhea(10.3%). We strongly recommend that pediatricans take a more forceful role in increasing the rate of breast-feeding. Since. as our study showed, the obsticales to breast-feeding are based in misinformation, the pediatrician through education and reinforcement is in a strong position to bring about change.

Alkylhalide가 Mouse의 LDH isozyme 분포에 미치는 영향 : Cellulose Acetate Electrophoresis Method

권미경,김영옥,박은주,이동화,이명미,이문희,이양자 曉星女子大學校 藥學大學 學生會 1988 曉星藥誌 Vol.4 No.-

The effect of Carbontetrachloride administration on Lactatedehydrogenase isozyme patterns were analyzed by cellulose acetate electrophoresis for the brain, heart, kidney, lung, liver and mouse muscle. Each other tissue was found to have a characteristic distribution of these isozymes. At normal condition, aerobic tissue such as brain, heart, kidney, and lung were found to have all five isozymes from LDH_1 to LDH_5, while anaerobic tissues such as liver and muscle were found to have superiorly LDH_5 The toxicity of Carbontetrachloride administration was most prominence in the liver, and lung toxicity was occured also.

특수체육활동(EWHAPA)이 학령기 발달장애아동의 기본운동기술과 스포츠 기능습득에 미치는 영향

홍양자,이은경 이화여자대학교 동작과학연구소 2007 이화체육논집 Vol.10 No.-

본 연구는 특수체육활동이 학령기 발달장애아동의 기본운동기술과 스포츠 기능습득에 영향을 주는지 파악하고 효과적인 특수체육활동을 제공해 주는데 목적이 있다. 연구대상은 서울지역 소재의 일반초등학교에 재학중인 발달장애아동(10-14세)으로 이화여자대학에서 시행 중인 프로그램에 참여하고 있는 아동 14명 중 최종실험에 참여 한 9명의 아동(남9명, 여:2명)이다. 전문가를 통해 검증 받은 특수체육활동을 매주 1회씩 12주간 90분 동안 실시하였다. 연구도구는 TGMD II와 이화여자대학 특수체육연구회에서 8년 동안 사용하고 전문가를 통해 검증받은 검사지를 수정 보완한 운동기능검사지를 사용하였다. Spss 12.0을 이용하여 비모수통계 Wilcoxon test와 비모수 상관분석 Spearman Correlation을 하였다. 이 연구를 통해 얻은 결과는 특수체육활동은 학령기 발달장애아동의 기본운동기술을 향상시키는데 긍정적인 효과가 있었지만 스포츠 기능습득을 향상 시키는 데는 통계적으로 유의한 차이가 없었다. 또한 특수체육활동은 기본운동기술 중 이동운동기술과 스포츠 기능습득 간에 그리고 물체조작 기술과 스포츠 기능습득 간에 상관관계가 있었다. The purpose of this study was comprehend and investigate the effects of adapted physical activity program on basic motor skill and sports skill of school-aged children with developmental disorder. The subjects were 9 children(M-7; F:2, age:10-14) who participated in this study to the end of 14 children. Basic motor skill was tested by TGMD II and sports skill was tested by probated sports skill test paper. The collected data were analyzed by wilcoxon test and spearman correlation using Spss 12.0. The results were as follows; 1. The adapted physical activity program showed higher scores than pre-test one in basic motor skill and sports skill, however, it showed without significant level. 2. The adapted physical activity program was showed correlation between the locomotor skill of basic motor skill and the sports skill except running. 3. The adapted physical activity program was appeared correlation between the object control skill of basic motor skill and the sports skill.

창원지역 일부 초등학교 고학년 학생의 전통발효식품에 대한 기호도와 영양지식 조사연구

송양순,허은실,이경화 창원대학교 생활과학연구소 2000 생활과학연구 Vol.4 No.-

The purpose of this study was to investigate Food Preference and Nutritional Knowledge for Traditional Fermented foods that was basic data to develope nutrition education program and to increase consumption of traditional fermented foods. The questionnaires have been collected from 229 fourth, fifth and sixth grade students of one elementary in Changwon. The results of this study were summarized as follows. 70.1% of students answered that they like ganjang, and that was higher in male compared to female(p<0.05). 82.2% of students answered that they like kochujang, and the hot taste of the kochujang was main reason of their like of kochujang and it was the most important reason for those also dislike of kochujang. 57.4% of students like deonjang, and that was higher in male than female same as ganjang(p<0.05). The reason for favorite deonjang was 'superior taste', 'great nutrient' and 'frequent consumption', and that was significantly defferent between gender. 84.2% of students answered that they like kimchi. The main reason for favorite kimchi was same as that of deonjang, and that dislike kimchi was 'hot taste' and 'inferior taste'. The student's most favorite food cooked with kimchi were stir fried kimchi rice and kimchi stew. The Student wanted taste of kimchi which was mild hot, hot and mild sweet, 85.6% of students prefer sikhe. The main reason for like sikhe was 'superior taste' and that dislike sikhe was 'inferior taste'. Most parents used to traditional fermented foods in meal preparation. The mean of nutrition knowledge was 6.03, and that was not significantly defferent between gender. Consequently these date suggest that education program to increase of nutrition knowledge and consumption of traditional fermented food is required for elementary school students.