Nutlin-3 induces HO-1 expression by activating JNK in a transcription-independent manner of p53

CHOE, YUN-JEONG,LEE, SUN-YOUNG,KO, KYUNG WON,SHIN, SEOK JOON,KIM, HO-SHIK Spandidos Publications 2014 International journal of oncology Vol.44 No.3

A recent study reported that p53 can induce HO-1 by directly binding to the putative p53 responsive element in the HO-1 promoter. In this study, we report that nutlin-3, a small molecule antagonist of HDM2, induces the transcription of HO-1 in a transcription-independent manner of p53. Nutlin-3 induced HO-1 expression at the level of transcription in human cancer cells such as U2OS and RKO cells. This induction of HO-1 did not occur in SAOS cells in which p53 was mutated and was prevented by knocking down the p53 protein using p53 siRNA transfection, but not by PFT-alpha, an inhibitor of the transcriptional activity of p53. Accompanying HO-1 expression, nutlin-3 stimulated the accumulation of ROS and the phosphorylation of MAPKs such as JNK, p38 MAPK and ERK1/2. Nutlin-3-induced HO-1 expression was suppressed by TEMPO, a ROS scavenger, and chemical inhibitors of JNK and p38 MAPK but not ERK1/2. In addition, nutlin-3-induced phosphorylation of JNK but not p38 MAPK was inhibited by TEMPO. Notably, the levels of nutlin-3-induced ROS were correlated with the mitochondrial translocation of p53 and this induction was prevented by PFT-beta, an inhibitor of the mitochondrial translocation of p53. Consistent with the effect of the ROS scavenger and MAPK inhibitors, PFT-beta reduced HO-1 expression and the phosphorylation of JNK induced by nutlin-3. In the experiments of analyzing cell death, the knockdown of HO-1 augmented nutlin-3-induced apoptosis. Collectively, these results suggest that nutlin-3 induces HO-1 expression via the activation of both JNK which is dependent on ROS generated by p53 translocated to the mitochondria and p38 MAPK which appears to be stimulated by a ROS-independent mechanism, and this HO-1 induction may inhibit nutlin-3-induced apoptosis, constituting a negative feedback loop of p53-induced apoptosis.

Antioxidant and hepatoprotective effects of Korean ginseng extract GS-KG9 in a D-galactosamine-induced liver damage animal model

Yun Ho Jo,Hwan Lee,Myeong Hwan Oh,Gyeong Hee Lee,You Jin Lee,Ji Sun Lee,Min Jung Kim,Won Yong Kim,Jin Seong Kim,Dae Seok Yoo,Sang Won Cho,Seon Woo Cha,Mi Kyung Pyo 한국영양학회 2020 Nutrition Research and Practice Vol.14 No.4

BACKGROUND/OBJECTIVES: This study was designed to investigate the improvement effect of white ginseng extract (GS-KG9) on D-galactosamine (Ga1N)-induced oxidative stress and liver injury. SUBJECTS/METHODS: Sixty Sprague-Dawley rats were divided into 6 groups. Rats were orally administrated with GS-KG9 (300, 500, or 700 mg/kg) or silymarin (25 mg/kg) for 2 weeks. The rats of the GS-KG9- and silymarin-treated groups and a control group were then intraperitoneally injected Ga1N at a concentration of 650 mg/kg for 4 days. To investigate the protective effect of GS-KG9 against GalN-induced liver injury, blood liver function indicators, anti-oxidative stress indicators, and histopathological features were analyzed. RESULTS: Serum biochemical analysis indicated that GS-KG9 ameliorated the elevation of aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), and lactate dehydrogenase (LDH) in GalN-treated rats. The hepatoprotective effects of GS-KG9 involved enhancing components of the hepatic antioxidant defense system, including glutathione, glutathione peroxidase (GPx), superoxide dismutase (SOD), and catalase (CAT). In addition, GS-KG9 treatment inhibited reactive oxygen species (ROS) production induced by GalN treatment in hepatocytes and significantly increased the expression levels of nuclear factor erythroid-2-related factor 2 (Nrf2) and heme oxygenase-1 (HO-1) proteins, which are antioxidant proteins. In particular, by histological analyses bases on hematoxylin and eosin, Masson"s trichrome, α-smooth muscle actin, and transforming growth factor-β1 staining, we determined that the administration of 500 mg/kg GS-KG9 inhibited hepatic inflammation and fibrosis due to the excessive accumulation of collagen. CONCLUSIONS: These findings demonstrate that GS-KG9 improves GalN-induced liver inflammation, necrosis, and fibrosis by attenuating oxidative stress. Therefore, GS-KG9 may be considered a useful candidate in the development of a natural preventive agent against liver injury.

심근 허혈-재관류 백서 모델에서 mitogen-activated protein kinase 활성화에 대한 α-lipoic acid의 효과

김연경,박은미,최준호,윤경호,유남진,이은미,김남호,정진원,김민선,박병림,오석규 圓光大學校 醫科學硏究所 2006 圓光醫科學 Vol.21 No.1

급성 뇌경색 환자에서 경색 위치가 심근 효소치 상승에 미치는 영향

윤경호,박현영,박성욱,장혁,김요식,조광호,유남진,김남호,오석규,정진원 圓光大學校 醫科學硏究所 2003 圓光醫科學 Vol.18 No.2

고혈압 환자들의 건강행태

김석범,강복수,윤성호,황정희,이경수 韓國保健敎育.健康增進學會 2000 보건교육건강증진학회지 Vol.17 No.1

This study was conducted to examine health behaviors in hypertensive patients and the factors that would affect these healthy life-styles, and to provide basic data for effective and continuous hypertension control in private medical facilities. Among those patients diagnosed as hypertension who had visited the Department of Internal Medicine of Yeungnam University Hospital during the two month period from August 2 to September 30, 1999, the present study included 222 patients who were in more than one month after the initial diagnosis of hypertension and those who had hypertension for less than 10 years. Using a structured questionnaire, the present study was conducted by a self-administered survey method, and the obtained data were analyzed with t-test, χ2-test and one-way analysis of variance using the SPSS statistical program. The scores on knowledge related to hypertension were higher as the education level of a patient was higher(p〈0.05). As for occupation, those who performed office or management jobs showed higher scores than those who were into manufacturing jobs, and as for economic status, although those patients who were in middle class showed highest scores, however, no statistical significance was observed. The scores of knowledge on hypertension was higher when the family history hypertension was present(p〈0.05). The scores of health-related behaviors were higher with higher education level and higher economic status, higher in those with family history of hypertension, and higher in those with office or management jobs than those who had manufacturing jobs(p〈0.05). Blood pressure measurement on regular basis was performed most frequently in those who were between 50∼59 years old with 83.3% and was least frequent in those who were older than 70 years old with 50%(p〈0.05). The frequency of regular blood pressure measurement was higher with higher education level, higher economic status, higher in those with family history of hypertension, and the highest in those with management position with 93.5%, however, on statistical significance was observed. Changes in health-relaxed behaviors after hypertension diagnosis were higher with higher education level, higher economic status, and in those patients performed office or management work. In particular, diet change was observed in female and higher economic status and smoking cessation was observed in 60∼69 years old. Housewives and office workers or managers have taken exercise more regularly and those who had management jobs and had high scores on knowledge related to hypertension would participate health education program more actively. Thus, for improving health-related behaviors for continuous management of hypertension, changes in health-related behaviors can be followed through conduction health education to improve understanding of knowledge related to hypertension as the method of helping to improve changes in health life-styles in those with little education and those in low economic status.

수냉쿨러의 냉각 효율 향상을 위한 플레이트 부품의 형상에 관한 연구

성윤호,김형찬,김선민,윤석영,이영웅,이상욱 순천향대학교 부설 산업기술연구소 2021 순천향 산업기술연구소논문집 Vol.27 No.2

This study investigates changes in cooling efficiency for heat sink plate width, perforated width, and chamfer angle as part of a parameter study to maximize cooling efficiency of heat sink by setting various variables on plates. The width of the plate is set to 16, 20, 24, 28 mm, perforated width 1.5, 2.0, 2.5 mm, chamfer angles 5, 10, 15 and 20°. By conducting a computational study with Ansys Fluent, the sink plate with plate width of 16mm, perforated width of 3mm and chamfer angle of 10° is shown to be the best cooling efficiency.

DSK50을 이용한 16Kbps ADPCM 구현

한경호,조윤석 단국대학교 1998 論文集 Vol.32 No.-

ITU G.721, G.723 standard ADPCM algorithm is implemented by using Tl's fixed point DSP start kit (DSK). The ADPCM can be implemented on a various rates, such as 16Kbps, 32Kbps and 40Kbps. The ADPCM is a sample based compression technique and its complexity is not so high as the other speech compression techniques such as the other speech compression techniques such as CELP, VSELP and GSM, etc. and can be implementd on a low cost DSP kit. ADPCM is widely applicable to most of the low cost speech compression application such as the tapeless answering machine, simultaneous voice and fax modem, digital phone, etc. TMS320C50 DSK system has a TMS320C50 DSP is a low cost fixed point DSP chip and an AIC (analog interface chip) which operates as a single chip A/D and D/A converter with 14 bit resolution. ADPCM C code is compiled by TI TMS320C50 C-compiler and implemented on the DSK on-chip memory. Speech signal input is converted into 14 bit linear PCM data and encoded into ADPCM data and the data is sent to PC through RS232C. The ADPCM data on PC is received by the DSK through RS232C and then decoded to generate the 14 bit linear PCM data and converted into the speech signal. The DSK system has audio in/out jack and we can input and output the speech signal. The simulation is performed on Pentium PC with C-code executable files. The result of speech is compared with the original speech. It is performed encoding on DSK and decoding on PC and the result is audible but not so good as that of PC simulation.

建蘭 Rhizome의 繼代培養에 關한 硏究 : Ⅰ.MS基本培地에서 生長調節物質과 Sucrose의 添加量이 Rhizome의 增殖에 미치는 影響 Ⅰ.The effects of growth regulating substance and addition quantity of sucrose on proliferation of rhizom in M.S basic culture ground.

姜鎬宗,朴相眩,蔡潤錫 진주산업대학교 1984 論文集 Vol.22 No.-

東洋系 Cymbidium屬인 建蘭의 rhizome을 大量 증식할 目的으로 MS基本 培地에 生長調節 物質의 滴定濃度와 Sucrose의 濃度를 알고저 本試驗을 遂行하였던 바 그 結果는 다음과 같다. 1. 生長調節劑의 濃度에 關係없이 Sucrose의 濃度가 2%정도일 때 rhizome의 증식이 양호하였다. 2. Sucrose의 濃度가 3% 以上이며 NAA 濃度가 1ppm이상 일때는 rhizome이 짧아지고 數도 현저히 감소하는 현상이었다. 3. 엽 분화는 대게 rhizome이 신장하여 많은 마디를 形成하고 1.5㎝이상 자랐을 때 그 선단에서 잎이 분화하였다. 4. rhizome을 치상할 때 횡식(橫殖)으로 치상하면 個體數가 증가하며 上向性의 경향이고 종식(縱殖)할 경우는 rhizome이 세장하면서 下向性의 경향이었으나 대체로 下向性이었다. This experiment was carried out to find the optimum concentration of growth regulate substrates and sucrose for mass production of the orient ochid plant (cymbidium ensifolium) and the result was follows. 1. Whether the growth regulate substrates were applied or not the growth of rhizome of tested orchid was increased in the treatment of 2% Sucrose. 2. When sucrose concentration in more than 3% and NAA is more than 1ppm in the media the length of rhizome become shoter and the number of rhizome decreased. 3. After the rhizome growth up to 1.5㎝ highet in length and formed a number of nodes the leaves were seperated from the tip of the rhizomes. 4. When the rhizome were planted horizontally the number of plants increased and the rhizome growed in the air, where as when the rhizome was planted verfically it become thin and most of rhizome growed in the media

Cytokinin류의 경엽처리가 봄감자 동화물질 전류에 미치는 영향

蔡潤錫,姜鎬宗 진주산업대학교 2001 論文集 Vol.40 No.-

Effect of Cytokinins(Kinetin and Benzyl adenin :BA) on the yields and carbohydrats and nitrogen compounds in the different parts of the spring potatoes as Stadies. Both kinetin and BA caused sisnifican (P <0.05) increare in yields, (carbohydrats and nitrogen compounds in the plant) while no sisnificar increares were observed in treatments the average tubes weights per plant were increased incread in low concentration of kinetin treatment where are in hight concentration of BA. Starch conteints in leaf treated with kinetin and BA showed lower than that of the control, and in stem 1∼2% higher than that of the control the starch con in treat the control. The starch sugar contents in the tuber treated with kinetins and BA has no significan difference between the treatment and the control. The free sugar contents in the leaf, freated with kinetins showed me much significan differentes between the treatment and the control.

딸기 育苗方法에 관한 硏究 : (Fragaria grandiflora E.)

姜鎬宗,蔡潤錫,李相雨 진주산업대학교 1998 論文集 Vol.37 No.-

"아끼히메"의 공중육묘에 관한 기초자료를 얻기 위해 7월 10일에 조사하여 정리한 실험결과를 요약하면 다음과 같다. 1. 모주의 분지 발생율은 편아가 6.3%, 2아가 36.8%, 3아가 44.3%, 4아가 12.4% 그리고 5아가 0.2%로 나타났다. 2. 주당 런너발생수는 1아에서 7.33개, 2아에서 14.67개, 3아에서 20.00개, 4아에서 24개가 발생되었으며, 분지당 런너발생수는 6.0∼7.3개였다. 3. 런너당 자묘발생수는 1.4∼3.0주 였다. 4. 묘의 차수별 중량은 1차 자묘가 5.23g, 2차 자묘가 4.52g, 3차 자묘가 2.85g, 4차 자묘가 1.37g으로 나타났다. 5. 삽목시 발근에 소요되는 일수는 10∼14일이었다. This study aims at obtaining basic data on aerial raising of strawberry seedling, 'AKIHIME', The results of study are as follows: 1. The ratio of ramification from stock plant was 6.3% in the first shoot, 36.8% in the second, 44.3% in the third, 12.4% in the fourth and 0.2% in the fifth, respectively. 2. The number of runners per plant was 7.33 in the first shoot, 14.67 in the second, 20.00 in the third and 24.00 in the fourth, and the number of runners per branch was 6.0˚≠7.3. 3. The number of daughter seedlings per runner was 1.4˚≠3.0. 4. The fresh weight at each stage of seedling was 5.23 at the first daughter seedling stage, 4.52g at the second, 2.85g at the third, and 1.37g at the fourth. 5. 10-14 days were required for rooting after seedlings had been cut.