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        Nutlin-3 induces HO-1 expression by activating JNK in a transcription-independent manner of p53

        CHOE, YUN-JEONG,LEE, SUN-YOUNG,KO, KYUNG WON,SHIN, SEOK JOON,KIM, HO-SHIK Spandidos Publications 2014 International journal of oncology Vol.44 No.3

        A recent study reported that p53 can induce HO-1 by directly binding to the putative p53 responsive element in the HO-1 promoter. In this study, we report that nutlin-3, a small molecule antagonist of HDM2, induces the transcription of HO-1 in a transcription-independent manner of p53. Nutlin-3 induced HO-1 expression at the level of transcription in human cancer cells such as U2OS and RKO cells. This induction of HO-1 did not occur in SAOS cells in which p53 was mutated and was prevented by knocking down the p53 protein using p53 siRNA transfection, but not by PFT-alpha, an inhibitor of the transcriptional activity of p53. Accompanying HO-1 expression, nutlin-3 stimulated the accumulation of ROS and the phosphorylation of MAPKs such as JNK, p38 MAPK and ERK1/2. Nutlin-3-induced HO-1 expression was suppressed by TEMPO, a ROS scavenger, and chemical inhibitors of JNK and p38 MAPK but not ERK1/2. In addition, nutlin-3-induced phosphorylation of JNK but not p38 MAPK was inhibited by TEMPO. Notably, the levels of nutlin-3-induced ROS were correlated with the mitochondrial translocation of p53 and this induction was prevented by PFT-beta, an inhibitor of the mitochondrial translocation of p53. Consistent with the effect of the ROS scavenger and MAPK inhibitors, PFT-beta reduced HO-1 expression and the phosphorylation of JNK induced by nutlin-3. In the experiments of analyzing cell death, the knockdown of HO-1 augmented nutlin-3-induced apoptosis. Collectively, these results suggest that nutlin-3 induces HO-1 expression via the activation of both JNK which is dependent on ROS generated by p53 translocated to the mitochondria and p38 MAPK which appears to be stimulated by a ROS-independent mechanism, and this HO-1 induction may inhibit nutlin-3-induced apoptosis, constituting a negative feedback loop of p53-induced apoptosis.

      • SCIESCOPUSKCI등재

        Antioxidant and hepatoprotective effects of Korean ginseng extract GS-KG9 in a D-galactosamine-induced liver damage animal model

        Yun Ho Jo,Hwan Lee,Myeong Hwan Oh,Gyeong Hee Lee,You Jin Lee,Ji Sun Lee,Min Jung Kim,Won Yong Kim,Jin Seong Kim,Dae Seok Yoo,Sang Won Cho,Seon Woo Cha,Mi Kyung Pyo 한국영양학회 2020 Nutrition Research and Practice Vol.14 No.4

        BACKGROUND/OBJECTIVES: This study was designed to investigate the improvement effect of white ginseng extract (GS-KG9) on D-galactosamine (Ga1N)-induced oxidative stress and liver injury. SUBJECTS/METHODS: Sixty Sprague-Dawley rats were divided into 6 groups. Rats were orally administrated with GS-KG9 (300, 500, or 700 mg/kg) or silymarin (25 mg/kg) for 2 weeks. The rats of the GS-KG9- and silymarin-treated groups and a control group were then intraperitoneally injected Ga1N at a concentration of 650 mg/kg for 4 days. To investigate the protective effect of GS-KG9 against GalN-induced liver injury, blood liver function indicators, anti-oxidative stress indicators, and histopathological features were analyzed. RESULTS: Serum biochemical analysis indicated that GS-KG9 ameliorated the elevation of aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), and lactate dehydrogenase (LDH) in GalN-treated rats. The hepatoprotective effects of GS-KG9 involved enhancing components of the hepatic antioxidant defense system, including glutathione, glutathione peroxidase (GPx), superoxide dismutase (SOD), and catalase (CAT). In addition, GS-KG9 treatment inhibited reactive oxygen species (ROS) production induced by GalN treatment in hepatocytes and significantly increased the expression levels of nuclear factor erythroid-2-related factor 2 (Nrf2) and heme oxygenase-1 (HO-1) proteins, which are antioxidant proteins. In particular, by histological analyses bases on hematoxylin and eosin, Masson"s trichrome, α-smooth muscle actin, and transforming growth factor-β1 staining, we determined that the administration of 500 mg/kg GS-KG9 inhibited hepatic inflammation and fibrosis due to the excessive accumulation of collagen. CONCLUSIONS: These findings demonstrate that GS-KG9 improves GalN-induced liver inflammation, necrosis, and fibrosis by attenuating oxidative stress. Therefore, GS-KG9 may be considered a useful candidate in the development of a natural preventive agent against liver injury.

      • 수냉쿨러의 냉각 효율 향상을 위한 플레이트 부품의 형상에 관한 연구

        성윤호,김형찬,김선민,윤석영,이영웅,이상욱 순천향대학교 부설 산업기술연구소 2021 순천향 산업기술연구소논문집 Vol.27 No.2

        This study investigates changes in cooling efficiency for heat sink plate width, perforated width, and chamfer angle as part of a parameter study to maximize cooling efficiency of heat sink by setting various variables on plates. The width of the plate is set to 16, 20, 24, 28 mm, perforated width 1.5, 2.0, 2.5 mm, chamfer angles 5, 10, 15 and 20°. By conducting a computational study with Ansys Fluent, the sink plate with plate width of 16mm, perforated width of 3mm and chamfer angle of 10° is shown to be the best cooling efficiency.

      • KCI등재

        고혈압 환자들의 건강행태

        김석범,강복수,윤성호,황정희,이경수 韓國保健敎育.健康增進學會 2000 보건교육건강증진학회지 Vol.17 No.1

        This study was conducted to examine health behaviors in hypertensive patients and the factors that would affect these healthy life-styles, and to provide basic data for effective and continuous hypertension control in private medical facilities. Among those patients diagnosed as hypertension who had visited the Department of Internal Medicine of Yeungnam University Hospital during the two month period from August 2 to September 30, 1999, the present study included 222 patients who were in more than one month after the initial diagnosis of hypertension and those who had hypertension for less than 10 years. Using a structured questionnaire, the present study was conducted by a self-administered survey method, and the obtained data were analyzed with t-test, χ2-test and one-way analysis of variance using the SPSS statistical program. The scores on knowledge related to hypertension were higher as the education level of a patient was higher(p〈0.05). As for occupation, those who performed office or management jobs showed higher scores than those who were into manufacturing jobs, and as for economic status, although those patients who were in middle class showed highest scores, however, no statistical significance was observed. The scores of knowledge on hypertension was higher when the family history hypertension was present(p〈0.05). The scores of health-related behaviors were higher with higher education level and higher economic status, higher in those with family history of hypertension, and higher in those with office or management jobs than those who had manufacturing jobs(p〈0.05). Blood pressure measurement on regular basis was performed most frequently in those who were between 50∼59 years old with 83.3% and was least frequent in those who were older than 70 years old with 50%(p〈0.05). The frequency of regular blood pressure measurement was higher with higher education level, higher economic status, higher in those with family history of hypertension, and the highest in those with management position with 93.5%, however, on statistical significance was observed. Changes in health-relaxed behaviors after hypertension diagnosis were higher with higher education level, higher economic status, and in those patients performed office or management work. In particular, diet change was observed in female and higher economic status and smoking cessation was observed in 60∼69 years old. Housewives and office workers or managers have taken exercise more regularly and those who had management jobs and had high scores on knowledge related to hypertension would participate health education program more actively. Thus, for improving health-related behaviors for continuous management of hypertension, changes in health-related behaviors can be followed through conduction health education to improve understanding of knowledge related to hypertension as the method of helping to improve changes in health life-styles in those with little education and those in low economic status.

      • 삽목방법이 묘소질과 토마토 생육에 미치는 영향

        채윤석,박상대,강호종 진주산업대학교 농업기술연구소 2007 農業技術硏究所報 Vol.20 No.-

        본 시험은 60일 된 토마토 실생묘의 정단부를 제외하고 상위, 중위, 하위 각 2마디를 하나로, 푸르미, 토실이 용토에 마디삽목하여 묘의 소질을 조사하고, 육성된 마디삽목묘를 저단밀식 재배하여 토마토의 생육과 과실의 특성에 미치는 영향을 살펴본 결과는 다음과 같다 1. 삽목 10일과 30일 후 신초장 및 신초경의 생육은 하나로, 푸르미용토가 유의적인 차이가 있었으며, 20일 후 신초장과 신근은 푸르미용토에서 삽수채취 하위부분에서 길었다. 2. 최종수확기에서 잎수는 푸르미상토가 많았고, 마디수는 중·하위절이 많았다. 초장과 엽장, 지상부 및 지하부 생체중과 건물중은처리간 유의성이 없었다. 3. 생육중기의 엽록소함량은 푸르미용토와 중·상위 절이 많았으나 최종수확기 무렵에는 처리구간 비슷하였다. This study was conducted to investigate the effects of the bedsoils(H,P and T) and the cutting sites the seedling quality, growth and fruit characters in the dense planting culture of tomato. The samples for test were prepared by dividing three parts(upper, middle and lower part) of 60days old healthy seedlings of tomatoes and collecting 2 modes from each part except the shoot position. The tomato's major characters, seedling quality, fruit character and soluble solids content were measured after 10, 20, and 30 days. 1. The new shoot length grew up bed soil of Hanaro, Promi but Hanaro soil thicken new shoot diameter and new root grew highly of promi soil, lower node in the 10 days after cutting. The growth of new shoot length in the 20 days after cutting grew up highly Promi soil and upper position node, middle position node, the growth of new root grew up highly Promi soil, middle position node and lower node. The growth of new shoot length in the 30 days after cutting grew up highly Hanaro soil and new root number was good enough in Hanaro soil and upper position, middle position node. 2. Leaf number was a lot in Promi soil at harvest time but node number was a lot in middle and lower position node. Flesh and dry weight of plant heigh, leaf, stem and root was similar. 3. The chlorophyll content of growing period on 20 May and 20 June was high in Promi soil and middle, lower position node but on 20 July was similar to all treatments.

      • 東洋系 Cymbidium屬 種子의 無菌發芽와 幼苗의 增殖體系 確立

        姜鎬宗,安相烈,蔡潤錫 진주산업대학교 1990 論文集 Vol.28 No.-

        동양계 Symbidium 속인 산천보세란 종자의 무균발아와 유묘증식을 위하여 근경형성을 위한 초기배양방법의 확립, 근경 및 유묘의 증식에 미치는 배지 및 Hormone제의 영향에 관한 실험을 수행한 결과를 요약하면 다음과 같다. 산천보세란 종자발아는 H_3P_4+NAA 0.1㎎/ℓ, Kinetine 0.01㎎/ℓ첨가배지에서 가장 양호하였으며 발아에 소요된 일수도 210일로서 가장 짧았다. 종자발아에서 얻은 근경의 재증식은 H_3P_4배지에 NAA 1㎎/ℓ, Kinetine 1.0~3.0㎎/ℓ첨가한 배지에서 양호하였으며, 유묘의 증식과 생장에 있어서는 H_3P_4+α-as-paratic acid 0.5㎎/ℓ, NAA 1.0㎎/ℓ, BA 3㎎/ℓ첨가 배지에서 가장 양호하였다. 지금까지의 실험을 종합하여 볼때 무균종자를 파종해서 발아시킨 후, 근경을 급속으로 증식시키고 H_3P_4+NAA 1.0㎎/ℓ함유배지에 BA 3㎎/ℓ을 첨가시킨 후 치상하여 multiple shooting을 시킨 다음 동일조성의 배지에 Hormone제를 제거하고 활성탄 1.6g/ℓ을 첨가한 배지에 이식해서 생장을 도모한 후 광도를 높이고 온도를 낮추며 일장을 16시간 정도 조절하여 순화시킨뒤 폿드 이식 가능한 유묘를 대량샌산 할 수 있을것으로 판단된다. In the process of nonsymbiotic germination and seedling multiplication of Sancheunboseran seed (temperate zone ochid, cymbidium sinense Willd); to establis the early culture method for rhizome formation and to examine the effect of various media and hormone drugs upon the multiplication of rhizome and seeding were the main prupose of this experiment. The results obtained are as follows: The medium with hyponex 3g/ℓ, peptone 4g/ℓ, NAA 0.1㎎/ℓ and Kinetine 0.01㎎/ℓ added was best for the seed germination for Samcheunboseran: the days required for germination were 210- the shortest of all mcdia. The remultiplication of rhzome acquired in the seed germination was most active in the medium with hyponex 3g/ℓ, peptone 4g/ℓ and kinetin 1~3㎎/ℓ added. The seedling showed the best multiplicatin and growth in the medium with hyponex 3g/ℓ, peptone 4g/ℓ, L-asparatic acid 0.5㎎/ℓ, NAA 1.0㎎/ℓ, and BA 3㎎/ℓadded. These results recommend us to plant the aseptic seed, to multiply rhizomes rapidly after germination, to plant the rhizome in the medium with hyponex 3g/ℓ, peptone 4g/ℓ, and NAA 1㎎/ℓ added, to remove the hormone drugs from the medium after multiple shooting and add charcoal(1.6g/ℓ) to the medium, and to trnsplant seedling to the medium. Such procedure will make it possible to produce pottable seedling massively.

      • Cytokinin류의 경엽처리가 봄감자 동화물질 전류에 미치는 영향

        蔡潤錫,姜鎬宗 진주산업대학교 2001 論文集 Vol.40 No.-

        Effect of Cytokinins(Kinetin and Benzyl adenin :BA) on the yields and carbohydrats and nitrogen compounds in the different parts of the spring potatoes as Stadies. Both kinetin and BA caused sisnifican (P <0.05) increare in yields, (carbohydrats and nitrogen compounds in the plant) while no sisnificar increares were observed in treatments the average tubes weights per plant were increased incread in low concentration of kinetin treatment where are in hight concentration of BA. Starch conteints in leaf treated with kinetin and BA showed lower than that of the control, and in stem 1∼2% higher than that of the control the starch con in treat the control. The starch sugar contents in the tuber treated with kinetins and BA has no significan difference between the treatment and the control. The free sugar contents in the leaf, freated with kinetins showed me much significan differentes between the treatment and the control.

      • 定植方法이 딸기의 發根및 生育에 미치는 影響

        姜鎬宗,朴相眩,蔡潤錫 진주산업대학교 농업기술연구소 2000 農業技術硏究所報 Vol.13 No.-

        In order to improve the method of raising seedling and planting in strawberry "jang hee", effect of pot shape and planting method upon the growth of the plants were investigated. The results were summarized as followings; 1. In general, root growth was better at round pot and I pot(conventional pot) than at other pots. Number of root was 26, the highest at round pot, and followed by 20 at I pot. Maximu root length was 29.4cm at round pot, longer than 26.5 at I pot. Root dry weight was 3.72g at I pot, and followed by 2.87g at round pot. 2. When the strawberry seedling adhering bed soil were planted uprightly, root number and root dry weigth were 17.7 and 1.63g respectively, a little higher than 17.0 and 1.45g at conventional method. However, maximum root length was 28cm at the conventional method, longer than 20cm at planting uprightly with bed soil. 3. Leaf area was 62.1㎠ at bed soil adhered and 46.2㎠ at bed soil removed. Petiol length was 1cm longer at bed soil adhered than at bed soil removed. Root dry weight was 4.7g at bed soil adhered and 1.6g at bed soil removed.

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