Phellinus linteus 균사 배양물로부터 분리한 단백다당체 Kp의 항암활성

정경수,김신숙,김희수,한만우,김병각 충남대학교 약학대학 의약품개발연구소 1994 藥學論文集 Vol.10 No.-

A protein-polysaccaride fraction Kp(53.9% polysaccharide, 14.2% protein) was separated from the shake-cultured mycelia of a basidiomycetous fungus, Phellinus linteus, and its antitumor activity against sarcoma 180 in ICR mice was investigated. When administered after the tumor implantation. Kp exerted antitumor activity by inhibiting the growth of the sarcoma 180 solid tumor by 71.5% and increasing the life span of the sarcoma 180 ascitic mice by 51.5% at 100 ㎎/㎏. In pretreatment tests, in which Kp was administered once daily for 9 days before the tumor implantation. Kp inhibited the growth of the solid and ascites form of sarcoma 180, respectively, by 35.4% and by 80.3% at 100 ㎎/㎏. However, Kp showed no in vitro cytotoxic activity against a murine leukemia L1210 and a human gastric tumor SNU.1 upto the concentration of 200 ㎍/㎖. From these results, it is clear that the antitumor activity of Kp is exerted through its immunomodulating activity on the host's immune system.

政黨의 法的 規制에 관한 硏究

鄭萬喜 東亞大學校 1982 東亞論叢 Vol.19 No.1

세자리 Schiff base Cobalt(Ⅱ)와 Nickel(Ⅱ) 착물들의 합성과 전기화학적 성질

정만태,이송주,채희남,정병구 광주보건대학 1994 論文集 Vol.19 No.-

Co(Ⅱ) and Ni(Ⅱ) complexes containing tridentate Schiff base ligand SOIPH [salicylidene-o-iminopyridine] derived from salicylaldehyde with 2-aminopyridine were synthesized. These complexes were characterized 표 elemental analysis, thermogravimetric analysis(T.G.A.), solution conductivity, and IR and UV-visible spectra. It was found that Co(Ⅱ) and Ni(Ⅱ) complexes had a 2 : 1 Schiff base ligand to metal(Ⅱ) ratio and were octahedral configuration. The redox processes of these complexes in DMF and DMSO containing 0.1M TEAP as supporting electrolyte were investigated at glassy carbon electrode by cyclic voltammetry (CV) and at HMDE(hanging mercury drop electrode) by differential pulse polarographic (DPP) technique. Reduction of Co(Ⅱ) complex was two steps, whereas Ni(Ⅱ) complex was one step. Reduction of these complexes observed as one electron process irreversibly and diffusion- controlly. Reduction of the Co(Ⅱ) complex was observed positive potential than Ni(Ⅱ) complex.

대구지역 치과의사들의 누적외상성 장애에 대한 조사

전만중,사공준,이중정,이희경,정종학 大韓産業醫學會 2001 대한직업환경의학회지 Vol.13 No.1

목 적 : 치과의사를 대상으로 누적외상성 장애의 증상유병률 및 양상을 조사하고 그 관련요인을 규명하여, 다양한 직종별 데이터 베이스 구축에 기초자료 제공 및 직종별 비교를 통한 누적왁상성 장애의 예방대책수립에 도움이 되고자 시행하였다.방 법 대구광역시에서 진료행위를 하고 있는 치과의사 512명을 대상으로 인구학적 요인과, 진료관련특성, 사회심리적요인, 자각증상에 대해 설문 조Al하였다. 사회심리적 특성으로는 직무요구도(5항목), 정신신체적 긴장도(5항목), 직무불만족도를 측정하였으며(Karasek, 1994), A형 행동양상도 함께 측정 하였다.자각증상에 대한 조사는 송동빈 등(1997)에 의해 개발된 자각증상 설문지를 이용하였으며 목, 어깨, 팔 및 팔꿈치, 손목, 손가락, 등에 통증이나 쑤심, 저림등의 불편한 증상과 관련된 변수들을 조사하였다.조사된 결과는 N10SH의 근골격계장애에 대한 감시를 위 한 기준을 적용하여 분석하였다.결 과 : N10SH 감시기준에 의한 누적외상성 장애 증상호소율이 63.3 %로 높게 나타났으며, 그 증상호소부위는 어깨, 목, 허리, 손과 손목, 무릎, 발과 발목의 순이였다. 진료위치는 9시에서 10시 방향에서의 접근이 가장 많았으며 이들 중 어깨 부위의 증상호소율이 가장 높았다.근무경력은 5년에서 10년 사이가 가장 많았으며 이들도 목과 어깨부위의 증상호소율이 가장 높았다.대상자들이 주로 보는 환자에 따른 증상 호소율은 치주치료를 주로 하는 경우 증상호소율이 가장 높았으며, 연령별로는 30~◎세에서 증상호소율이 70 %로가장 높았다. 성별로는 여성에서 증상호소율이 높았으며, 미흔인 대상자에서 증상호소율이 높게 나타났다. 일일 진료환자수에 따른 증상호소율은 21명 이상을 진료하게 되는 군에서 70 %로 가장 높았다.로지스틱 회귀분석을 한 결과 정신신체적긴장도와직무요구도가 높을수록 누적외상성 장애의 증상호소가 높은 것으로 나타났다. 또한 A형 행동양상을 보일수록증상호소가 높았으며, 쳐근 3개월 동안에 진료위치를 변경한 경우도 증상호소가 높은 것으로 조사되었다.결 론 : 조사한 일반적, 직업적 특성, 진료판련특성 및 사회심리학적 특성을 독립변수로 하여 다변량로지스틱 회귀분석 결과 여성에서 누적 외상장애증상발현위험이 남성에 비해 높은 것으로 조사되었으며,정신신체적 긴장이 높을수록, A형 행동양상 일수록누적외상성 장애증상 발현위험이 높았다. 위의 결과로 볼 때 치과의사의 경우 숙련되고 섬세한 근골격계운동을 지속적으로 반복해야 한다는 특성파 불안정한자세를 장시간 취해야 한다는 작업적 특성으로 인한근골격계 장애의 발생은 물론이고 환자 만족도에도신경을 써야하는 등의 정신적 스트레스도 누적외상성장애를 일으킬 수 있는 위험요인이라 할 수 있겠다. Objective : To investigate the symptom prevalence and aspects of symptoms for cumulative trauma disorders in dentists and elucidate their associated factors. Methods : A questionnaire-based investigation on demographic factors, work-related characteristics, psychosocial factors and symptoms of 512 dentists practicing in Daegu metropolitan city was conducted. Subsets of psychosocial characteristics, including job demand(5 items), psychosomatic strain(5 items), job insecurity, and type A behavior were also assessed. For the evaluation of symptoms early developed questionnaire by Song (1997) was used to investigate the parameters related to pains in the neck, shoulder, arm and elbow, wrist, finger, back or discomforts, such as pricking and numbing. Results : The symptom prevalence of cumulative trauma disorders, calculated based on NIOSH criteria, was high at 63.3%; the most frequent site of complaint was the shoulder, followed by neck, back, hand and wrist, knee, foot and ankle. The most common approach position was direction of 9 to 10 o'clock. Most subjects had 5-10 years of work experience and those with such years of experience also complained most frequently of symptoms in the neck and shoulder areas. As to rate of symptom complaint according to the type of dental procedure, the highest rate was associated to periodontic treatment. According to age, those between the age of 30-40 had the highest symptom prevalence rate at 70 %. According to sex, women had the higher symptom prevalence rate. Unmarried subjects also had higher symptom prevalence rate. The results of logistic analysis showed that the higher the psychosomatic strain and job demand, the higher the symptom prevalence rate of cumulative trauma disorders. Moreover, the higher the score of type A behavior, the higher the symptom prevalence rate, and even those who changed the location of their practice for the last three months also showed high symptom prevalence rate. Conclusions : The results of multiple logistic regression analysis, which incorporated demographic, work-related and psychosocial characteristics as independent variables, showed that women were at a higher risk than men in developing cumulative trauma disorders; and that the higher the psychosomatic strain and more frequent type A behavior, the higher the risk of developing cumulative trauma disorders. The above results seem to suggest that not only continual, repeated fine and skillful musculoskeletal movements and lengthy hours in an uncomfortable position but also the psychological stress related to patient satisfaction, can lead to cumulative trauma disorders.

野球選手 傷害에 關한 調査 硏究

李鎔滿,元忠熙 公州大學校 스포츠科學硏究所 1991 스포츠科學硏究所論文集 Vol.- No.4-5

Ⅰ. Purpose ; The major purpose of this paper is to provide data not only to improve performance of the player and to discover a modern, Scientific, and psychological training method suitable for the high-standards and delicacy modern sports, but also to find a better way of improving the physical status and the injury treatment of the middle-school players through a survey of their major causes and treatments of injuries in base-ball games. Ⅱ. Method For the studies and analysis of cause of injuries and its treatment in baseball. this report was completed through the survey of 486 players form 27 middle school teams. The survey was made form November 1988 to september 1990 and its results of analysis are as follows Ⅲ. Result 1) The frequency according to the time is the order of afternoon morning and nights respectively and according to events, it is in the order of practice official games and personal practice respectively 2) The events of injury occurs mostly in defense practices and last in personal practices. In the defense of official games, the major cause of injuries are by thrown of batted balls and injury caused by collision to fenses are the least Injuries in offense are caused majorly by pitched of thrown balls and the injuries caused by obstacles such as bats are considered the least causes. 3) The injured parts of the body are in the order of arms, waist, leg, foot and finally the neck. 4) the muscular injuries are the major & injuries followed by skin injuries and respectively. 5) In the case of first-aid, self-treatment was the leading treatment and given by doctors at the ball - park was the least common case And in the case of treatments after injuries hospital treatments was the most common case 6) In the case of medical treatments, hospital treatments and bone-setting clinic treatments were the most and the least common case of treatment respectively.

감잎(Diospyros kaki folium)의 Polyphenol 화합물군이 면역기능에 미치는 영향

배만종,성태수,최청,안봉전,박무희 경산대학교 생명자원개발연구소 1999 생명자원과 산업 Vol.3 No.-

한국산 감잎(Diospyros kaki folium)으로부터 polyphenol 화합물을 분리하여, 감잎의 기능성 식품으로서의 근거를 제시하고자, 면역기능의 활성화에 관한 실험을 행한 결과 다음과 같은 결과를 얻었다. 감잎의 폴리페놀 화합물이 면역기능에 미치는 영향에 관한 실험에서는 감잎으로부터 분리된 폴리페놀 화합물 Ⅰ, Ⅱ 및 Ⅲ에 비하여 시료를 투여한 군에서 항체생산이 증가되었으며, 특히 폴리페놀 화합물 Ⅱ와 Ⅲ에서 높은 유의성을 나타내었다. Rosette 형성의 결과에서도 감잎의 폴리페놀 화합물 Ⅱ와 Ⅲ을 투여한 군에서 분리된 비장과 복강 상재성 마크로파지에서 rosette 형성능이 높은 것으로 나타났다. Candida에 대한 시료의 탐식능을 관찰한 결과, 대조군에 비하여 fraction Ⅰ은 70%, Ⅱ는 76.8% 및 Ⅲ은 93%의 탐식능의 효과를 나타내었다. 또한, 면역기능을 담당하는 기관인 비장에 관한 세포증식능을 실험한 결과 대조군에 비하여 시료를 투여한 모든 군에서 비장세포가 증가하였으며, 시료의 농도가 증가할수록 세포증식도 증가되는 경향을 나타내었다. This study was conducted to investigate immuno functional activity of the ployphenol fractions isolated from persimmom leaves(Diospyros kaki folum). In the experiment of rosette forming cell, the results showed that all the polyphenol fractions enhanced the cell count compared with the control group. Especially polyphenol fraction Ⅱ and Ⅲ showed highly significant effect. The phagocytic activity of peritoneal macrophage on mice was significantly increased by the polyphenol fractions of the persimmon leaves compared with that of the control group. The gagocytic activity of polyphenol fraction Ⅰ, Ⅱ and Ⅲ oagainst the C. parapsilosis was 26.4%, 72% and 82.3% respectively. The pagocytic activity of the same fractions against the C. albicans was 70%, 76.8% and 93% respectively.

부산지역 무균성 뇌막염 원인 바이러스의 분리 및 동정 : 1998년을 중심으로

김영희,정영기,김기순,지영미,윤재득,김병준,구평태,민상기,정구영,김만수,조경순 동의대학교 기초과학연구소 2000 基礎科學硏究論文集 Vol.10 No.1

The incidence of aseptic meningitis infection is ensuing and threatening the health of children. Enteroviruses are the major agents of aseptic meningitis and identification of virus has been a clue to diagnosis and epidemiology. The outbreak of aseptic meningitis occurred in Pusan, 1998. Patients were concentrated from April through November. Children were more susceptible than adults. Among 306 cases of specimens from stool, throat swab tested, only 7.2% were positive on virus isolation, 12 cases from stool and 10 from throat, respectively. All isolated 7 serotypes of viruses represented cytopathic effect on cultured cells. Three types of echovirus 6, 25, 30 and coxsackievirus B2, B3, B4, B6 were identified by neutralizing anti body test. Isolated coxsackievirus and echovirus were observed by an electron microscope with negative staining.

고등균류의 CYTKINE 분비 및 세포증식에 미치는 영향

배만종,최청,박무희,이재성 경산대학교 생명자원개발연구소 1998 생명자원과 산업 Vol.2 No.-

담자균 유래의 신규 생리활성 물질의 탐색의 일환으로 상황버섯과 버들송이의 균사체로부터 세포내단백다당류와 세퐁외단백다당류로 분리 및 정제된 시료를 가지고 세포증식, IL-2 alc IL-4 생성의 경향에 대한 실험을 행한 결과는 다음과 같다. 1. DNA 합성과 MTT에 의한 세포증식의 결과에서는, 상황버섯과 버들송이 세포배양외액에서 추출·분리된 땅류가 균사체에서 보다 비장세포에 대한 세포활성이 높은 것으로 났다. 2. IL-2 생성 결과에서는 상황과 버들송이 균사체 및 세포외액으로부터 추출된 다당류의 농도가 10∼300㎍/mL의 범위에서 IL-2 생성이 증가되었으며, 균사체 배양외액으로부터 분리된 다당류에서 높으로 것으로 나타났다. 3. IL-4 생성의 결과는 상황버섯과 버들송이로부터 추출된 세포내 및 세포외 단백다당류가 대조군에 비행서 높은 것으로 나타났다. The polysaccharide, intracellurar and extracellular, extracts from the liquid culture of the Phellinius igniarius and the Agrocybe cylindracea were purifide. This study was conducted to investigated which were measured the proliferative response to mitogens of splenocyte and production of IL-2 and IL-4. The results obtained from the experiment as follows : The proliferation of splenocyte was increased mosteffectively when the extracellua polysaccharide extracted from the Phellinius igniarius and the Agrocybe cylindracea were administrated to the mice. And the extractcellular polysaccharide extract from the Phellinius igniarius was proliferation activity increased according to culture time. The IL-2 secreased, a marker increased when 48hrs. culture with the extracellula polysaccharide extracted from the Phellinius igniarius and the Agrocybe culindracea(10∼100㎍/mL). And there was significantly the extracellula polysacchride extracted from the Phellinius then the extracellula polysaccharide extracted from the Agrocybe cylindracea. IL-4 production was significantly increased compared with the control group.

길경이 알레르기 저감화에 미치는 영향

배만종,박무희,최청 경산대학교 생명자원개발연구소 1996 생명자원과 산업 Vol.1 No.-

BALB/C 마우스에 egg-albumim 알레르기 항원을 제조해서 3차에 걸쳐 항원을 접촉시켜 알레르기를 유발하는 과정에 길경으로부터 추출한 열수 추출물(PRAE), 50% 메탄올 추출물(PRME)을 반복해서 투여하여 이들에 대한 항알레르기 작용을 검토하기 위해서 PCA 반응과 혈중 histamine 함량을 측정하였던 바 다음과 같은 결과를 얻었다. 1. IgE 생성 억제 효과를 검정하기 위한 PCA값은 50% 메탄올 추출물이 대조군에 비해서 4∼8배로 억제되어 IgE 생성에 있어서 가장 뛰어난 억제효과를 보였으며, 열수추출물과 아세톤추출물도 다소 억제효과가 있었다. 또한 아세톤추출물은 1차 항원에 의한 유발 시에는 변화를 보여주지 않았으나, 2차, 3차 항원 유발에 대해서는 현저하게 IgE 생성이 억제되었다. 2. 혈중 histamine 함량은 대조군이 80.8㎍/㎖∼105.8㎍/㎖인 반면, 메탄올추출물은 35.5㎍/㎖∼45.3㎍/㎖으로 PCA와 동일하게 가장 현저한 억제효과를 보였으며, 아세톤추출물은 PCA 결과와 유사한 양상으로 2차, 3차 항원 유발에서 현저한 억제현상을 보였다. 이상의 결과를 볼 때 메탄올추출물이 PCA값과 혈중 histamine 생성을 가장 억제하였으며, 그 다음은 아세톤추출물로서 이들에 대해서는 기능성 식품으로서의 개발이 기대되며 활성물질의 화학구조 분석과 사이토카인에 대한 실험도 요구된다고 하겠다. In order to research for compound of immunomodulatic and anti-allergic function. These experiments were conduced to investigated the effects of hot water extract(PRWE) and acetone extracted(PRAE) from platycodi radix on immune response. The effect of these platycodi radix extracts on passive cutaneous anaphylaxis(PAC) and histamine was investigated by using BALB/C mice. The results obtained from this study are as follows. In the experiment of PCA, the results of experimental groups were suppression level as compared with the control group. And the PRME group was showed remarkably decreased than that of control group. The histamine content in blood was that PRME (35.5㎍/㎖∼45.3㎍/㎖) showed remarkably showed a remarkably suppression effect as compared with the control group.

팥의 RAPD 분석 조건 최적화 연구

이충열,박현철,박진철,김성만,김용철,최재희,최인수 밀양대학교 농업기술개발연구소 2000 農業技術開發硏究所報 Vol.4 No.1

The object of this study was to optimize PCR condition for RAPD analysis in adzuki bean. The best template DNA concentration was 20ng(0.25unit taq polymerase and 2.5mM MgCl2), 40ng(1unit taq polymerase and 2.5mM MgCl2, 1unit taq polymerase and 4.5mM MgCl2 and lunit taq polymerase and 7.0mM MgCl2), and 60ng(0.5unit taq polymerase and 2.5mM MgCl2, 0.5unit taq polymerase and 4.5mM MgCl2, 1unit taq polymerase and 2.5mM MgCl2, and 1unit taq polymerase and 4.5mM MgCl2) The best MgCl2 concentration was 2.5mM(40ng template DNA and 1unit taq polymerase, 60ng template DNA and 0.5unit taq polymerase, and 60ng template DNA and 1unit taq polymerase), 4.5mM(20ng template DNA and 0.25unit taq polymerase, 60ng template DNA and 0.5unit taq polymerase, and 60ng template DNA and 1unit taq polymerase), and 7.0mM(40ng template DNA and 1unit taq polymerase). Amount of taq polymerase was 0.25unit(20ng template DNA and 2.5mM MgCl2), 0.5unit(60ng template DNA and 2.5mM MgCl2, 60ng template DNA and 4.5mM MgCl2) and 1unit(40ng template DNA and 2.5mM MgCl2, 40ng template DNA and 4.5mM MgCl2, 40ng template DNA and 7.0mM MgCl2, 60ng template DNA and 2.5mM MgCl2, 60ng template DNA and 4.5mM MgCl2, and 60ng template DNA and 7.0mM MgCl2). When we consider results from template DNA concentration, MgCl2 concentration, and amount of taq polymerase, the best condition for PCR optimization was 60ng template DNA, 4.5mM MgCl2, and 1unit taq polymerase. Reaction temperatures for the optimal PCR condition were 84℃, 32℃, 62℃; 90℃, 40℃, 72℃; and 92℃, 36℃, 72℃.