한국산 산사자 추출물을 이용한 화장품 소재 개발

안봉전,강보연,이진태 대한본초학회 2002 大韓本草學會誌 Vol.17 No.2

Objectives : This is the study of the application as the ingredients of cosmetics through the examination of the function for physiological activity of Crataegi Fructus, which has been used for herb medicines since ancient times. Method : Korean Crataegi Fructus was extracted by water and ethanol and it was concentrated for the experiment materials. As the stability of the physiological activity function experiment, the effect of the electronic donating ability, antioxidative activity and UV scanning depending on various moisturizers has been researched for the development of cosmetic ingredients. Results : The ethanol extract form Crataegi Fructus showed similar antioxidative activity, reaching 81.2% at 1000 ppm. As the result of UV scanning of each extract at 1000 ppm concentration and in UV 200~400 nm, it showed the similar absorbent peak (O.D: 0.7~0.8) to green tea extract, rosemary extract in UVB zone (280 nm) and the high optical density (1.8~2.4) in UVC zone. Propylene Glycol showed 71.8~75% antioxidative activity at 1mg/mL, and Glycerin showed 70.7%~71.8%, while 1,3-Butylene Glycol showed the safest antioxidative activity, reaching 79%~85%. Conclusion : According to the result from the electronic donating ability measure of Normal skin-softener of the basic formulation, using 1,3-Butylen Glycol and various extracts from natural plants, it can be proved that Crataegi Fructus extract has the similar antioxidative activities degree of other extracts.

Studies on Biological Activity from Extract of Crataegi Fructus

안봉전,이진태 대한본초학회 2002 大韓本草學會誌 Vol.17 No.2

춘계총회 및 제 20 차 학술발표회 - 화학 / 분석 / 생리활성분야 [ P - 2 ]: 한국산 오이풀의 생리기능효과 및 화장품소재로서 응용

안봉전,김준흥,김치봉,이창언,곽제훈,박정미,이진태 한국식품저장유통학회 ( 구 한국농산물저장유통학회 ) 2002 한국식품저장유통학회 학술대회 Vol.- No.-

내소황련탕(內疎黃連湯) 및 구성약재의 항산화효과 검증과 항암 및 항균효과

안봉전,이창언,손준호,이진영,박태순,박정미,배호정,편정란 대한본초학회 2005 大韓本草學會誌 Vol.20 No.4

포스터 발표논문 초록 ( 식품분석 / 품질 분야 Poster 초록 ) : HPLC분석에 의한 녹차의 Polyphenol 성분 조사

안봉전,배만종,우희섭,최청 한국응용생명화학회(구 한국농화학회) 1997 한국응용생명화학회 학술발표회 Vol.1997 No.1

한국산 녹차로부터 Angiotensin Converting Enzyme 저해제 분리 및 화학구조

안봉전 경산대학교 생명자원개발연구소 1998 생명자원과 산업 Vol.2 No.-

고혈압 예방을 위해 우리는 한국산 녹차로부터 두 종류의 축합형 탄닌을 분리하고 기기분석에 의해 구조를 결정한 결과 prodelphinidin B-2 3-O-gallate와 prodelphinidin B-2 3,3'-O-digallate였다. ACE 저해 효과에서 B환에 galloyl이나 gallate 결합한 prodelphinidin류가 저해활성이 증가하는 것으로 확인되었다. Prodelphinidin B-2 3,3'-O-digallate는 50μmole에서 약 84% 저해 효과가 관찰 된 바 이것은 산업화의 가능성을 확인시켜주었다. For the prevention of dental caries, two kinds of novel condensed tannin were isolated from Korean green tea and characterterrized prodelphinidin B-2 3-O-gallate and prodelphinidin B-23,3'-O-digallate, respectively, by mechanical analysis. Effect of inhibition on the Angiotensin Converting Enzyme was shown that B-ring, gallyol or gallate compound in prodelphinidins, grown the effect of inhibition. Prodelphinidin B-2 3,3'-O-digallate inhibited about 84% at 50 μmole, is shown a possibility that is used to industry.

한국산 감잎로부터 Polyphenol계 생리활성물질 분리

안봉전,배만종,최희진,장운빈,성태수,최청,An, Bong-Jeun,Bae, Man-Jong,Choi, Hee-Jin,Zhang, Yun-Bin,Sung, Tae-Soo,Choi, Cheong 한국응용생명화학회 2002 한국농화학회지 Vol.45 No.4

옛부터 민간 약제로 사용했던 감나무 잎을 식품, 화장품 기능 생물 신소재로 활용하기 위해 감나무잎의 주성분으로 알려진 폴리페놀 화합물을 분리하고 생리활성 기능을 조사하였다. 감나무 잎을 60%의 아세톤으로 추출한 용액을 동결 건조시켜 농도별로 조제하고 glucosyltransferase, tyrosinase 효소저해를 관찰한 결과 glucoslytransferase는 $1.8{\times}10^{-1}$ mg/ml에서 82.84% 저해를, tyrosinase는 0.8 mg/ml에서 21,65% 저해 활성이 확인되었다. Sephadex G-50으로 gel filtration한 결과 fraction 1, 2, 3, 4, 5로 분획되었고 이중 fraction 1, 2에서 효소 저해 활성을 나타내었다. 활성 분획물의 물질군을 확인하기 위해 proteinase K 처리와 멸균처리한 결과 효소저해에 영향은 없어 활성물질군은 단백질군이 아니며, UV spectrum에서 자외선 영역에 최대 흡광치를 보여주므로 페놀링을 갖는 화합물로 추정하게 되었다. 추정화합물을 Sephadex LH-20, MCI-gel 및 Bondapak $C_{18}$ column에서 분리한 결과 F1 fraction에서 compound $1{\sim}4$가지 4종류의 화합물, F2 fraction에서는 compounds 5, 6, F3 fraction에서 compound 7, 8, 9를 순수 정제하여 재결정하였다. 분리된 화합물은 TLC 발색반응에서 flavan-3-ol 골격을 기본으로 하는 축합탄닌 반응을 보였고 전개정도에서 monomer가 2종류, dimer가 3종류, trimer가 1종류로 확인되었다. We purified polyphenols from persimmon leaf and tested their biological activity. The 60% acetone extract was lyophilized and applied to test enzyme inhibition of glucosyltransferase and tyrosinase. GTase was 82.4% inhibited at $1.8{\times}10^{-1}$ mg/ml and tyrosinase 21.7% inhibited at 0.8 mg/ml. The acetone extract was fractionated into F-1, 2, 3, 4, 5 by Sephadex Q-50 gel filtration and the fraction-1 and 2 showed higher enzyme inhibition activity than the other fractions. To the Proteinase K treatment and autoclaving of the two fractions had no effect on the enzyme activity, but these results suggested that active fraction was not protein but phenol ring completed compounds. By Sephadex LH-20, MCI-gel and Bondapak $C_{18}$ column chromatographies, compouds 1, 2, 3 and 4 from F-1 fraction, compounds 5 and 6 from F-2 fraction and compounds 7 , 8 from F-3 fraction were purified and re-crystallized. The purified compounds was assumed to be condensed tannins of frame flavan-3-ol frame on the basis of color reagent reaction and to be a mixture of monomer, dimer and trimer according to TLC analysis.

Jack Fruit잎으로부터 Tyrosinase저해제 분리 및 구조

안봉전 東國專門大學 1994 金龜論叢 Vol.2 No.1

Tyrosinase 저해물질을 Jack fruit 옆으로부터 Sephedex G-50, Sephadex LH-20으로 분석한 결과 B-fraction의 1mM에서 약 30-40%의 저해 효과가 있었다. 순수분리 된 화합물을 기기분석한 결과 afezelechin-(4α→8)-catechin으로 동정되었고 이 화합물의 IC_(50)은 0.6mM로 확인되었다.

복합처방인 승마갈근탕(升摩葛根湯)의 항산화 및 항균효과

안봉전,이진영,배호정,박태순,김태완,문두환,권오준,손준호,이창언,박근혜,김한혁 한국응용생명화학회 2010 Journal of Applied Biological Chemistry (J. Appl. Vol.53 No.1

Biological activities such as anti-oxidative and anti-microbial of the Seungmakalgeuntang, a tradi-tional prescription, were evaluated. The electron donating ability of water, ethanol, supercritical fluid and 1,3-butylene glycol extract of Seungmakalgeuntang showed more than 50% at a 100 ppm con-centration. At a 1000 ppm concentration, the superoxide dismutase-like activities of ethanol and supercritical fluid extract of Seungmakalgeuntang showed less than 50%. xanthine oxidase inhibition effect of the supercritical fluid extract showed more than 70% at a 1,000 ppm concentration, which was higher than vitamin C. From the measurement on lipid oxidation, the Fe 2+ chelating abilities of the supercritical fluid extract of Seungmakalgeuntang was more than 60% at a 100 ppm concentration. Also the Cu 2+ chelating abilities of supercritical fluid extract Seungmkalgeuntangwas showed more than 60% at a 500 ppm concentration. Clear zones formed by sample against the human skinresident microflora such as Staphylococcus epidermidis, Staphylococcus aureus and Propionibacterium acne of ethanol and supercritical fluid extract of Seungmakalgeuntang showed the highest among all the extracts tested using a 4㎎/disc. The minimum inhibitory concentration (MIC) against both S. epidermidis and S. aureus showed 2,500 ppm in the extract of the supercritical fluid. 복합처방인 승마갈근탕을 이용하여 화장품 및 식품에 응용하고자 항산화 및 항균효과를 검토하였다. 승마갈근탕의 전자공여능은 모든 추출물 100 ppm에서 50% 이상의 효과를 나타내었고, 특히 초임계 추출물의 경우 500 ppm에서 88%로 가장 높은 효과를 나타내었다. SOD 유사활성은 승마갈근탕 1,000 ppm에서 50% 이하의 다소 낮은 경향을 보였으나, 그 중 에탄올과 초임계 추출물의 경우 각각 46, 45의 효능을 나타내었으며, xanthine oxidase 저해활성의 경우 초임계 추출물 1,000 ppm에 서 76%로 대조군인 vitamin C의 39%보다 높은 효과를 나타내 었다. 승마갈근탕의 지방산패 억제능을 측정하기 위해 산화촉 진제인 Fe2+, Cu2+ 이온을 첨가하여 측정한 결과 초임계 추출물 의 Cu2+ 첨가 시 500 ppm에서 68%의 효과를 나타내었고, Fe2+를 첨가 시 100 ppm에서 60%의 지방산패 억제능을 나타내었다. 승마갈근탕의 clear zone 형성을 관찰한 결과, 추출물 모두 Escherichia coli를 제외한 모든 균주에 대해서 항균 효과가 나타내었다. Staphylococcus aureus와 Propionibacterium acnes의 경우 4 ㎎/disc에서 초임계 추출물에서는 1.45, 2.10 ㎝로 가장 높은 항균 효과를 나타내었고, Staphylococcus epidermidis의 경우, 에탄올 추출물이 1.82 ㎝의 항균효과를 나타내었다.

감잎(Diospyros kaki folium) 으로부터 분리한 Polyphenol 화합물의 카드뮴 제거효과

안봉전,최청,배만종,손준호,최희진,박무희,우희섭,배두경,조국영 한국농화학회 2000 Applied Biological Chemistry (Appl Biol Chem) Vol.43 No.3

In order to investigate the removal effect of cadmium by polyphenol compound extracted from persimmon leaves(Diospyros kaki folium), animal test was done. Two fractions such as F-1 and F-2 from persimmon leaves were compared with their safety and function. The food intake of group F-1 and F-2 considerably decreased within 1% level. Cadmium addition influenced to rat growth(a tested animal), but food efficiency ratio(FER) wasn't shown any considerable difference in F-1 and F-2, as compared to the control. Cadmium content of liver, kidney and femur considerably decreased within 1% level in F-1 and F-2, compared to the control, cadmium content of liver decreased 25% in F-1, 28% in F-2 also decreased 22% and 25% in kidney. In femur, also decreased 53% in F-1 and 59% in F-2 respectively. The test of cadmium content in feces indicate that the content considerably increased within 1% level in both group F-1 and F-2, as compared to the control(42% in F-1, 54% in F-2). As shown the above results, have seen the removal effect of cadmium by polyphenol compound.