急性骨髓性 白血病에서의 試驗管內 集落細胞 形成에 관한 연구

고석만,조덕연,박철산,강원권,김민범,김삼용,노홍규 忠南大學校 癌共同硏究所 1991 癌共同硏究所 硏究誌 Vol.1991 No.-

急性骨髓性 白血病에서의 試驗管內 集落細胞 形成에 관한 연구

고석만,조덕연,박철신,강원권,김민범,김삼용,노흥규 충남대학교 의과대학 지역사회의학연구소 1988 충남의대잡지 Vol.15 No.2

To evaluate the in vitro granulocyte-macrophage colony formation in acute myfloblastic leukemia and the prognostic implications of these results, the author performed the in vitro agar culture of bone marrow cells in 10 patients with acute myeloblastic leukemia (AML) and 5 control subjects. Culture medium was composed of 20% fetal calf serum(FCS), 50% Iscove's medium, 0.3% agar, 10% colony stimulation factor (CSF), and 2 × 10 exp (5) cells/ml. Human placental conditioned medium (HPCM) and phytohemagglutinin-leukocyte conditioned medium (PHA-LCM) were used as colony stimulating factor. Colony counting was done on 7th day of culture. Colony was defi ned as containg 20 or more cells, and cluster was defined as containing 3-19 cells. The results were as followings, 1. In control subjects, the number of clusters formed was 3-47/2 × 10 exp (5) cells (20±19)and that of colonies was 5-24/2 × 10 exp (5) cells (14±9) when stimulated with HPCM. When stimulated with PHA-LCM, the number of clusters formed 5-39/2 × 10 exp (5) cells(18±16) and that of colonies was 6-13/2 × 10 exp (5) cells (9±3). 2. In AML patients, 3 groups were recognized according to pattern of colony formation: 1) non-forming 2) cluster forming 3) both cluster and colony forming. Of 10 cases, 5 cases Were `non-forming', 2 cases were `cluster forming'; and 3cases wer cluster and `colony forming'; 3. All 5 cases 'non-forming' cases, one of 2 cases of `cluster forming'; and none of 3 cases of `cluster and colony forming' achieved complete remission. So, there was significa nt difference in remission rate in the different growth types. These results suggest that granulopoiesis in AML patients is impaired and the pattern of in vitro CFU-L(colony forming unit-leukemia) formation has prognostic significance.

急性骨髓性 白血症에서의 試驗管內 集落細胞 形成에 관한 연구

고석만,조덕연,박철신,강원권,김민범,김삼용,노흥규 忠南大學校 癌共同硏究所 1991 癌共同硏究所 硏究誌 Vol.1 No.1

To evaluate the in vitro granulocyte-macrophage colony formation in acute myeloblastic leukemia and the prognostic implications of these results, the author performed the in vitro agar culture of bone marrow cells in 10 patients with acute myeloblastic leukemia (AML) and 5 control subjects. Culture medium was composed of 20% fetal calf serum(FCS), 50% Iscove's medium, 0.3% agar, 10% colony stimulation factor (CSF), and 2×105 cells/ml. Human placental conditioned medium (HPCM) and phytohemagglutinin-leukocyte conditioned medium (PHA-LCM) were used as colony stimulating factor. Colony counting was done on 7th day of culture. Colony was defined as containg 20 or more cells, and cluster was defined as containing 3 - 19 cells. The results were as followings, 1. In control subjects, the number of clusters formed was 3-47/2×10^(5) cells (20±19)and that of colonies was 5-24/2×10^(5) cells (14±9) when stimulated with HPCM. When stimulated with PHA-LCM, the number of clusters formed 5-39/2×10^(5) cells(18±16) and that of colonies was 6-13/2×10^(5) cells (9±3). 2. In AML patients, 3 groups were recognized according to pattern of colony formation : 1) non-forming 2) cluster forming 3) both cluster and colony forming. Of 10 cases, 5 cases were 'non-forming', 2 cases were 'cluster forming'; and 3cases wer cluster and 'colony forming'; 3. All 5 cases 'non-forming' cases, one of 2 cases of 'cluster forming'; and none of 3 cases of 'cluster and colony forming' achieved complete remission. So, there was significant difference in remission rate in the different growth types. These results suggest that granulopoiesis in AML patients is impaired and the pattern of in vitro CFU-L(colony forming unit-leukemia) formation has prognostic significance.

급성 심근경색증을 동반한 혈전성 혈소판 감소성 자반증 1예

이태희,김진혁,김완중,박지영,강흥선,조정휘,김권삼,송정상,배종화 대한순환기학회 2003 Korean Circulation Journal Vol.33 No.11

Original Articles : Antimicrobial Resistance and Virulence Genes Presence in Escherichia coli Strains Isolated from Gomso Bay, Korea

( Kwon Sam Park ) 한국수산과학회(구 한국수산학회) 2013 Fisheries and Aquatic Sciences Vol.16 No.4

In total, 131 Escherichia coli isolates from surface seawater of the Gomso Bay, of Korea, were analyzed for their susceptibility to 22 different antimicrobials and for genes associated with antimicrobial resistance and virulence. According to the disk diffusionsusceptibility test, the resistance to tetracycline was most prevalent (33.6%), followed by that to ampicillin (22.1%), ticarcillin (22.1%), and trimethoprim (16.8%). More than 46.6% of the isolates were resistant to at least one antimicrobial, and 22.9% wereresistant to three or more classes of antimicrobials; these were consequently defined as multidrug resistant. We further found that 29 ampicillin-resistant isolates possessed genes encoding TEM-type (93.1%) and SHV-type (6.9%) β-lactamases. Amongthe 44 tetracycline-resistant isolates, tetA and tetC were found in 35 (79.5%) and 19 (43.2%), respectively, whereas tetB was detected in only three isolates (6.8%). With regard to virulence genes, merely 0.8% (n = 1) and 2.3% (n = 3) of the isolates werepositive for the enteroaggregative E. coli-associated plasmid (pCVD432) gene and the enteropathogenic E. coli-specific attaching and effacing (eae) gene, respectively. Overall, these results not only provide novel insight into the necessity for seawater sanitation in Gomso Bay, but they help reduce the risk of contamination of antimicrobial-resistant bacteria.

Antimicrobial Resistance and Virulence Genes Presence in Escherichia coli Strains Isolated from Gomso Bay, Korea

Park, Kwon-Sam The Korean Society of Fisheries and Aquatic Scienc 2013 Fisheries and Aquatic Sciences Vol.16 No.4

In total, 131 Escherichia coli isolates from surface seawater of the Gomso Bay, of Korea, were analyzed for their susceptibility to 22 different antimicrobials and for genes associated with antimicrobial resistance and virulence. According to the disk diffusion susceptibility test, the resistance to tetracycline was most prevalent (33.6%), followed by that to ampicillin (22.1%), ticarcillin (22.1%), and trimethoprim (16.8%). More than 46.6% of the isolates were resistant to at least one antimicrobial, and 22.9% were resistant to three or more classes of antimicrobials; these were consequently defined as multidrug resistant. We further found that 29 ampicillin-resistant isolates possessed genes encoding TEM-type (93.1%) and SHV-type (6.9%) ${\beta}$-lactamases. Among the 44 tetracycline-resistant isolates, tetA and tetC were found in 35 (79.5%) and 19 (43.2%), respectively, whereas tetB was detected in only three isolates (6.8%). With regard to virulence genes, merely 0.8% (n = 1) and 2.3% (n = 3) of the isolates were positive for the enteroaggregative E. coli-associated plasmid (pCVD432) gene and the enteropathogenic E. coli-specific attaching and effacing (eae) gene, respectively. Overall, these results not only provide novel insight into the necessity for seawater sanitation in Gomso Bay, but they help reduce the risk of contamination of antimicrobial-resistant bacteria.

사골동에서 발생한 양성골모세포종에 병발한 동맥류성 골낭종 1례

권삼현,박종권,문우성,권삼현,문우성 대한이비인후과학회 1999 대한이비인후과학회지 두경부외과학 Vol.42 No.4

Temperature-Dependency Urease Activity in Vibrio parahaemolyticus is Related to Transcriptional Activator UreR

Kwon Sam Park,Soo Jae Lee,Yong Hyun Chung,Tetsuya Iida,Takeshi Honda 한국미생물 · 생명공학회 2009 Journal of microbiology and biotechnology Vol.19 No.11

고농도 에탄올내성 초산균의 개발 및 배양특성

박권삼(Kwon-Sam Park),장동석(Dong-Suck Chang),조학래(Hak-Rae Cho),박욱연(Uk-Yeon Park) 한국식품영양과학회 1994 한국식품영양과학회지 Vol.23 No.4

식초 생산의 수율을 높이기 위해 재래식 식초생산에 사용되고 있는 종초로 부터 고농도의 에탄올에 내성을 나타내는 균주를 에탄올 순양법으로 개발해 내었다. 에탄올이 5~10% 첨가된 액체배지에 종초를 각각 가하여 에탄올 10%에서 증식이 가능한 균주를 순양 개시 42일만에 분리해 내었고, 이를 동정하여 Acetobacter sp. FM-10이라고 명명하였으며, 이 균주의 배양 특성을 살펴본 결과는 다음과 같다. Acetobacter sp. FM-10의 중식 최적조건을 살펴본 결과, 최적온도는 30℃였으며, 최적 pH는 5.0이었다. 초산생산에 적합한 배지의 조성은 에탄올 10%, 포도당 5%, yeast extract 1%가 함유된 배지였다. 식초생산 최적조건은 에탄올이 10% 첨가된 배지를 이용하여 30℃에서 정치배양시 배양 20일만에 최고 산도인 9.0%에 도달하였다. To increase the yield of acetic acid production, the author developed the bacterial strain which could grow well in high concentration of ethanol from the seed culture using in conventional vinegar production factory. By attenuation of the isolated strain in the broth media containing 5-10% ethanol, we could get the strain which could grow in the broth medium containing 10% ethanol. This strain was identified and named as Acetobacter sp. FM-10, and it's cultural characteristics were also investigated. The medium containing 10% ethanol, 5% glucose and 1% yeast extract was suitable for the acetic acid production with Acetobacter sp. FM-10. Optimum temperature and pH for the growth of Acetobacter sp. FM-10 were 30℃ and 5.0, respectively. The acidity of culture medium was reached to 9.0% after 20 days static cultivation at 30℃.

소규모 근린재생을 위한 가로주택정비사업 주거모델 개발

권혁삼(Kwon, Hyuck-Sam),이영환(Lee, Young-Hwan),박준영(Park, Joon-Young),김성훈(Kim, Sung-Hoon) 한국도시설계학회 2013 도시설계 : 한국도시설계학회지 Vol.14 No.6

본 연구는 대규모 정비사업의 대안으로 2012년 8월 시행된 가로주택정비사업의 관련법규와 적용대상지를 분석하고 적정한 사업규모와 주거모델을 제시하는 것을 목적으로 한다. 가로주택정비사업은 정비구역의 지정 절차 없이 일정요건을 갖춘 가로구역의 전부 또는 일부에 대해 7층 이하의 소규모 공동주택을 건설하여 주거환경을 개선하는 주민주도의 근린재생 수단이다. 그러나 실제 추진된 사례가 없고 구체적인 사업모델과 실행수단 등이 미흡하여 주민자력으로 사업을 추진하기에는 많은 한계가 있다. 이러한 배경에서 시뮬레이션을 통해 가로구역의 입지여건, 규모 및 접도조건에 따른 중층?고밀(5~7층, 용적률 200%)의 가로주택정비사업 주거모델 36개를 제시하고, 이를 실제 대상지에 적용하여 모델 적용성을 평가하였다. 제시된 가로주택정비사업 주거모델은 20~200세대의 접지성이 높은 가로대응형 중층 아파트로서 고층?고밀의 단지형 아파트에 비해 일조권?남향, 지하주차장 등 주거환경 측면은 미흡하지만, 소규모로 개발하는 만큼 지역의 장소적 특성과 커뮤니티를 유지하며 개발 할 수 있으며 원주민의 재정착에도 유리하다. 이들 주거모델은 가로구역의 조건에 따라 다양하게 조합하여 활용할 수 있으며, 이를 통해 주민들의 경제적 여건 등을 고려하여 소규모로 점진적이고 계획적인 근린재생을 유도할 수 있다. This study aims to propose new housing models for the redevelopment of residential blocks surrounded by streets, with the consideration on the current regulations of the redevelopment projects that are in effect from August 2012. In contrast to other redevelopment projects, the requirement of the procedures to determine the redevelopment areas are waved for the projects for residential blocks surrounded by streets. Thus, we conduct simulations to suggest 36 models of medium-rise and high-density housing (i.e., 5 to 7 story housings with floor area ratio of 2.0) that are applicable to various locations and sizes. Those models have 20~200 units per block, and, compared to high-rise and high-density housing, lack sun light exposure, and underground parking lots. Nevertheless, the proposed housing models produce affordable housings to contain existing economic situation of residents. The combination of those models would lead to a gradual and planned redevelopment for neighborhood renewal.