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      • KCI등재

        The Influence of Forest Fire Simulation on the Properties of Polymer Insulators

        이원교,한세원,최인혁,이동일 한국전기전자재료학회 2009 Transactions on Electrical and Electronic Material Vol.10 No.5

        Forest fire simulation tests were performed with polymer and porcelain insulators at GOCHANG Power Testing Center. These tests consisted of open flames causing a temperature rise of up to 600~800ºC measured at the insulator surfaces. Mechanical and electrical characteristics such as the specific mechanical load, the low frequency dry flashover voltage and the impulse flashover voltage were analyzed for the polymer insulators before, during and after the simulation tests and then compared to the porcelain insulators. At the end of the fire simulation tests, there was no detrimental deterioration of any of the insulators. All the insulators passed the KEPCO specification criteria. This study showed that the forest fire simulation had no impact on the polymer insulators.

      • 참전복(Haliotis discus hannai) 치패의 근육위축증 원인인 스쿠티카섬모충에 대한 Chitosan의 구충효과

        최상덕,이원교 여수대학교 1998 論文集 Vol.13 No.2

        최근 남해안 육상수조에서 사육중인 참전복(Haliotis discus hannai) 치패가 근육 위축, 탈색 및 움직임이 둔화되면서 폐사(근육위축증) 되어 문제의 심각성이 야기되어 원인생물의 구제에 관한 제반 연구가 필요한 실정이다. 본 연구에서는 스쿠티카섬모충에 대한 chitosan-oligosaccharides(OCHT)의 구제효과를 조사하였다. 항기생충 시험에 사용된 스쿠티카섬모충은 근육이 위축된 전복치패에서 검출된 것이다. 분리된 기생충은 서양배 모양이며, 내부에는 다량의 과립과 커다란 핵이 있으며, 구강은 배쪽에 있으며, 몸표면에는 크기가 같은 섬모가 있다. 충체의 전장은 24∼48㎛였다. 그리고 기생충의 구제시험에 사용된 OCHT은 96%의 탈아세틸화도와 7∼14당체였다. 배양된 기생충은 OCHT 300-500ppm시험구에서 30분이내에, OCHT 50-200ppm에서 1시간이내에 사멸되었다. 그리고 근육위축증이 발생한 전복 양식장에서 OCHT(200ppm, 1시간) 약욕에 따른 총 세균수는 3.2∼4.1×10⁴ CFU/㎖에서 3.4∼4.5×10² CFU/㎖로 감소하였으며, 해수 및 전복치패에서 스쿠티카섬모충은 검출되지 않았다. 한편, OCHT처리에 따른 스쿠티카섬모충의 사멸은 시간의 경과에 따라 섬모가 위축되거나 없어져 활동이 둔화되면서 타원형의 충체가 원형의 충체로 변했다. 원형의 충체는 세포질이 팽창되어 세포막이 파괴되어 죽거나 한쪽방향으로 세포막이 부풀어 올라 세포질이 이동된 후 세포막이 파괴되어 사멸되었다. 이것은 올리고키토산의 킬레이트화 등에 의한 기생충의 삼투압 상실로 기생충이 사멸된 것으로 추정된다. The purpose of this study was to confirm antiparasitical effect of chitosan oligosaccharides(OCHT) which was characterized by 96% deacetylation and 7-14 oligosaccharides, against scuticociliatids parasitic on cultured abalone, Haliotis discus hannai in a recirculation aquaculture system in southern Korea. The parasite was pear-shape, 24∼48㎛ in length, had many ciliam, contractile vacuole and nucles. Scuticodiliatids isolates were cultured and adjusted to 10⁴ individuals/㎖ in nutrient broth before adding OCHT diluted to 10, 50, 100, 200, 300, 400, and 500ppm. After the addition and incubation(20℃) of each diluted OCHT in the scuticociliatids-incubating flask, the parasites were died within 30 minutes in OCHT 300-500ppm, 60 minutes in OCHT 50-200ppm. When the treatment with OCHT was performed to get rid of the parasite in abalone cultured land-marine tank system, they were exterminated after 60 minutes in OCHT 200ppm. And there was apparent descreasing 3.2∼4.1×10⁴ CFU/㎖ to 3.4∼4.5×10 ² CFU/㎖ in the number of bacterial colonies. Until 60 minutes after the addition and incubation at OCHT 200ppm in the scuticociliatids-incubating fask, death process of the parasites was examined by time interval. The parasite showed non motility, withering of cillia, circle shape, extension of cell wall, movement of cellula tissue, destruction of cell wall. This indicated that formation of OCHT has a strong antiparasitical activity against scuticociliatids causing withering syndrome of abalone.

      • KCI등재

        Relationship between Ovarian Development and Plasma Levels of Steroid Hormones, and Induction of Oocyte Maturation and Ovulation in the Cultured Female Korean Sea Bass, Lateolabrax japonicus

        이원교,양석우,곽은주 한국발생생물학회 2000 발생과 생식 Vol.4 No.2

        양식산 농어, Lateolabrax japonius 암컷의 난소와 혈액을 1997년부터 1999년까지 매년 10월부터 2월까지 2회 반복 채취하였다. Gonadosomatic index는 11월부터 증가하기 시작하여 12월(12.81.5)과 1월(14.83.5)에 최고 수준을 나타낸 후 2월(2.61.5)에는 급격히 감소하였다(P<0.05). 난소내 난모세포는 12월과 1월에 3차 난황구기까지 발달하고 성장이 완료되지만, 성숙 및 배란이 되지 않고 2월 Gonad and blood samples were taken from the cultured female Korean sea bass, Lateolabrax japonicus from October to February between 1997 and 1999. Gonadosomatic index began to increase in November and reached the highest value in December (12.81.5) and January (14.83.5), and then decreased sharply in February (2.61.5, p<0.05). The ovarian oocytes developed to tertiary yolk stage and reached fully-Brown stage in December and January, and then underwent atresia without maturation and ovulation in February. The plasma estradio3-17 level increased from November, and reached the highest value in December (1,152.3107.2 pg/ml) and January (1,315.499.5 pg/ml), after then decreased in February (P<0.05). The concentration of plasma 17 ,20 -dihydroxy-4-pregnen-3-one was not significantly changed at low levels (86.66.5∼93.82.8 pg/ml) during the experimental period (P<0.05). All the fish with fully-grown oocytes in the ovary were matured and ovulated by HCG injection. The number of floating eggs were 325,00026,000 at HCG 1,000 luhg and 195,00035,000 at 2,000 lUikg. There was no difference in fertilization rate and hatching rate of the eggs (P<0.05). Considering these results, we could infer that the ovarian oocyte of the cultured Korean sea bass were not matured and ovulated because of the lack of gonadotropin surge. Moreover, HCG injection could induce oocyte maturation and ovulation in the cultured fish, and the effective dose was 1,000 IU/kg.

      • 성장중인 생쥐와 돼지난자의 성숙억제요인에 관하여

        이원교,권혁방 한국통합생물학회 1988 동물학회지 Vol.31 No.4

        세포융합방법을 사용하여 성장증인 포유동물의 난자에 들어있는 성숙억제요인(maturation inhibiting activity, 1연Al에 대해 조사하였다. 성장중인 생쥐난자와 성장한 미성숙난자를 1:1로 융합하여 배양했을 서 (14-17시간)에는 거의 모두 핵붕괴를 일으키었으나(90oyo), 2:1로 융합했을 때는 대부분(약 64%) 3개의 핵을 모두 간직하고 있었다. 돼지난자의 경우는 성장중인 것깎 성장한 것을 1:1로 융합하여 배양했을 때에도 융합체들은 모두 핵을 간직하고 있었으며 돼지의 성장중인 난자와 생쥐의 성장한 난자를 융합했을 때에도 모두 핵을 보존하고 있었다. 이에 반하여 돼지와 생쥐 모두에서 성장한 난자끼리 융합했을 때에는 예외없이 핵붕괴가 일어났다. 이러한 결과는 성장중인 생쥐나 돼지의 난자에 각IA가 존재한다는 열과 이종간에도 효과가 있다는 것을 보여주고 있다. 또한 이는 MIA와 성숙촉진요인(maturation promoting factor, MPH의 상대적인 양의 변화가 난자의 성숙조절에 증요한 9f할을 한다는 것을 시사해주고 있다.In an attempt to elucidate the nature of maturation inhibiting activity (MIA) in growing mamma-lian oocvtes, growing mouse and pig oocytes incompetent to resume meiosis were fused with fully grown immature oocvtes in various combinations and cultured for 14-17 hours. In slant cells composed of two mouse growing ooh임es and one large immature oocyte (2:기, their GVs remained well conserved (about 64%) after culture, but not in the ceils composed of one by one pairs. In giant cells of pig composed of one growing and onto large immature oocytes, both GVs remained conserved. In the cells composed of one pig growing and one mouse large oocytes, both GVs were also conserved. In contrast to this, pairs of large mouse oocvtes or those of large pig oocvtes had no CVs after culture. Thus, we could acertain the existEnce of MIA and none-pecificty of it in the mouse and pig growing oocvtes. The results also suggest that the relative amount of substances showlns MfA or MPF activity may be important in the regulation of oocyte amount of substances showing MIA or MPF activity may be important in the regulation of oocyte maturation.

      • KCI등재

        가공지선이 연결된 송전철탑의 탑각저항 측정에 관한 연구

        이원교,최종기,이영우,최인혁,김경철,Lee, Won-Kyo,Choi, Jong-Kee,Lee, Young-Woo,Choi, In-Hyuk,Kim, Kyung-Chul 한국전기전자재료학회 2010 전기전자재료학회논문지 Vol.23 No.1

        Footing Resistance of a 154 kV transmission towers in korea is commonly required to be less than 15 ohm to avoid lightning back-flashover accident. The periodic measurement of Footing Resistance is important to verify that the grounding performance of the towers has been maintained good. Towers are electrically connected in parallel with overhead grounding wire, therefore footing resistance of each tower will be measured after disconnecting the overhead ground wires from the towers. however, In this paper, three direct measurement methods of footing resistance are presented. There are very useful methods without disconnecting overhead ground wires from the tower under measurement. They are compared in KEPCO 154 kV transmission towers. The experimental results describe performances of them.

      • 붕어의 난황합성기 여포에서 스테로이드 생성에 관하여

        이원교 전남대학교 수산과학연구소 1993 수산과학연구소논문집 Vol.2 No.-

        Gonadosomatic index(GSI) of the silvercarp was investigated to clarified annual reproductive cycle from february to september in 1993. The GSI increased until march and the state of follicle was vitel10genic stage. The GSI fluctuated from april to july, it was evident that spawning of silvercarp occurred in those periods. The GSI decreased from october and the state of follical was atretic stage. Estradiol-17.8 ~) production was exam?ned from vitel10genic follicle to investigated steroid production ability in silvercarp. Isolated follicles were incubated for 18 hours in goldfish Ringer in presence or absence of human chorionic gonadotropin(HCG, 1IU, 10IU) or forskolin(adenylate cyclase stimulator, 0.1 μ M, 1 μM, 10 p.찌 and secretion of Ez by follicles was measured in media by radioimmunoassay. HCG(lIU, 101끼 and forskolin(0.1 μ M, 1 μM, 10 p.M) stimulated Ez secret?on and forskolin 10 μM was the most effect?ve in Ez secret?on. The time course of Ez secretion by follicles was investigated. Isolated follicles were ?ncubated presence or absence of HCG 10IU or forskolin 10 μM from 3 hour to 24 hour. The time course of HCG(10IU) stimulated Ez secretion was similar to that forskolin(lO μ씨 stimulated Ez secretion(3 hrs).

      • 개구리 난자의 성숙촉진요인에 관한 연구

        이원교,고선근,권혁방,Lee, Won-Gyo,Go, Seon-Geun,Gwon, Hyeok-Bang 한국통합생물학회 1992 동물학회지 Vol.35 No.1

        북방산개구리, 참개구리 및 옴개구리를 사용하여 성숙된 난자의 세포질에서 활성을 띠는 성숙촉진요인( maturation promoting factor, MPF)을 미세주입 법으로 확인하고 이들의 성질을 조사하였다. 핵붕괴(germinal vesicle breakdown, GVBD)된 난자의 세포질을 미성숙 난자(GV난자)에 주입하고(75-100 nl) 이들을 15-24시간 배양했을 때 대부분의 GV 난자들이 핵붕괴를 일으켰으나(약 80%) 미성숙 난자(GV 난자)의 세포질을 주입했을 때에는 약 200nl의 난자들만이 핵붕괴를 일으켰다. 핵붕괴된 난자들의 crude extract를 주입했을 때에도 역시 성숙유도 효과가 있었으며 이종간에도 효과가 있었다. 난자의 성숙을 잘 일으키지 않는 옴개구리의 난자를 사용하여 MPF를 가진 세포질을 계대주입(serial transfer)하였을 때에도 MPF가 계속 활성을 띠는 것을 확인할 수 있었다. 아울러 개구리 난자의 MPF생성과 증폭과정에 CAMP의 증가나 단백질 합성의 저해가 미치는 영향을 조사한 결과. MPF의 작용이 유의하게 이들에 의해 억제되는 것을 알 수 있었다.

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