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칼슘 알지네이트 젤에 고정화시킨 Invertase 에 의한 설탕의 가수분해
홍재식,변시명,엄태붕 한국농화학회 1984 Applied Biological Chemistry (Appl Biol Chem) Vol.27 No.2
Inverase was entrapped in calcium alginate gel. The immobilized beads had excellent uniform size and configuration. To screen the optimal conditions possessing high enzyme activity, effects of sodium alginate concentration, CaCl₂ concentration, and the incubation time of beads in CaCl₂ solution during the immobilization procedure were investigated. Immobilized beads prepared from the optimal conditions had 18.8 units per ㎖ of gel, which is equivalent to 68% of the activity of soluble enzyme. Several kinetic parameters were determined: Km value. 143 mM; optimum pH, 4.5; optimum temperature, 50℃. Enzyme loading capacity was 150 ㎎ per 20 ㎖ gel. No significant decrease in sugar conversion was observed during the column operation for 6 days.
한국산 느타리 버섯류 (Pleurotus sp.) 의 휘발성 향기성분
홍재식,정순택 한국균학회 1991 韓國菌學會誌 Vol.19 No.4
Volatile aroma concentrates of four oyster mushrooms cultivated in Korea were obtained by the simultaneous distillation-extraction method. The volatile components were identified by the combined capillary gas chromatography-mass spectrometry and compared with the of retention data on GC of those authentic compounds. About 54 volatile compounds were identified in each of the four edible oyster mushrooms. The main compounds in Pleurotus ostreatus ASI 201 were 1-octen-3-ol, 3-octanone, 3-octanol, in Pleurotus sajor-caju, 1-octen-3-ol, 3-octanone, 1,5-octadien-3one, 3-octanol, in Pleurotus florida-ostreatus-ostreatus, 1-octen-3-ol, n-hexanol, 3-octanone, 3-octanol, phenol, and in Pleurotus ostreatus (Ae-Neutari), 1-octen-3-ol, n-pentanal, n-hexanol, n-pentanol, 3-octanone, 3-octanol, 1,5-octadien-3-one, respectively. The mushrooms worked were rich in an alcohols and carbonyl compounds containing C_8 compounds. The peak area ratio of C_8 compounds in aroma concentrate were 56.60% in Pleurorus ostreatus ASI 201, 72.46% in Pleurotus sajor-caju, 54.84% in Pleurotus florida-ostreatus-ostreatus and 35.85% in Pleurotus ostreatus (Ae-Neutari), respectively.
홍재식,김명곤,김영회,이극로,이지열,정기태 한국균학회 1985 韓國菌學會誌 Vol.14 No.1
The volatile aroma concentrates were isolated from Pleurotus ostreatus by simultaneous distillation-extraction and analyzed by gas chromatography and combined gas chromatography-mass spectrometry. The aroma concentrates obtained had a typical raw or slightly cooked mushroom-like odor, and the yield was 123 ppm. Of 27 components identified, the major components were 3-octanone, 3-octanol, 2-octenal, n-octanol and 2, 4-decadienal, including 1-octen-3-ol contributing 67.06% of the total volatiles, and the C_8 compounds comprised about 80% of the total volatiles. On the other hand, the C_6 and C_8 alcohols in mushroom increased gradually with increase of standing time after homogenation, while aldehydes, ketones, and linoleic acid decreased, and also C_6 and C_8 compounds were significantly increased when linoleic and linolenic acid were added in homogenates. It appears that most of volatile aroma components in Pleurotus ostreatus were probably produced enzymatically from unsaturated fatty acids containing cis, cis-1, 4-pentadiene moiety.
홍재식,이종배,고무석,김정숙,이극로,정기태,Hong, Jai-Sik,Lee, Jong-Bae,Koh, Moo-Seok,Kim, Jeong-Sook,Lee, Keug-Ro,Jung, Gi-Tae The Korean Society of Mycology 1986 韓國菌學會誌 Vol.14 No.1
Pleurotus spp.중 섬유소(纖維素) 분해효소(分解酵素) 생산력(生産力)이 가장 강한 Pleurotus sajor-caju JAFM 1017을 합성배지(合成培地) 상에서 vitamin류(類), 무기염류(無機鹽類)와 배양조건(培養條件)의 영향을 검토(檢討)한 결과(結果)는 다음과 같다. 섬유소(纖維素) 분해효소(分解酵素) 생산(生産)은 folic acid와 thiamine-HCI에 의해 촉진되었고 $KH_2PO_4$와 $MgSO_4$의 최적농도(最適濃度)는 각각 0.2%, 0.04%(w/v)이었으며 그 밖의 무기염류(無機鹽類)는 효과(效果)가 없었다. 효소생산(酵素生産)에 최적(最適)인 배양온도(培養溫度)와 배지(培地) pH는 avicelase가 $25^{\circ}C$, 5.5이었고, CMCase는 $30^{\circ}C$, 5.0이 였으며, ${\beta}-glucosidase$는 $30^{\circ}C$, 6.5이었다. The production of cellulolytic enzymes by Pleurotus sajor-caju JAFM 1017 was stimulated by folic acid and thiamine-HCl. Among the inorganic salts, optimum concentrations of $KH_2PO_4$ and $MgSO_4{\cdot}7H_2O$ were 0.2% (w/v) and 0.04% (w/v), respectively, but other inorganic salts were not effective for the production of the enzymes. The optimum culture temperature and pH for the production were $25^{\circ}C$ and 5.5 for avicelase, and $30^{\circ}C$ and 5.0 for CMCase, and $30^{\circ}C$ and 6.5 for ${\beta}-glucosidase$, respectively.