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남성희 ( Nam Seong Hee ),이선주 ( Lee Seon Ju ),조세연 ( Cho Sae Yun ) 한국잠사학회 2002 한국잠사곤충학회지 Vol.44 No.1
눈꽃동충하초는 국내에서 몇 가지 학명으로 불려지고 있으나 그 중 오명이 포함되어 있음으로 인해 학자들간에 논란이 되고 있으며, 균학도들에 의해서 잘못 인용되는 사례가 빈번함에 따라 본 시험에서는 균을 재 동정코자 하였다. 수집균은 담황색 분생자병속을 1∼5여개 형성하며 그 끝마디에는 다량의 분생포자가 발생되었다. MA 배지 상에서는 25℃, 14일간 직경 2.1㎝로 더딘 생장을 보이며, colony의 색상은 앞면은 옅은 담황색, 뒷면은 연노랑 색이며, 분생포자는 타원형 혹은 난형으로 2.9∼6.6×1.5∼2.5 ㎛이며 분생포자를 지탱하고 있는 phialide는 부풀고 팽창한 형태로 3∼4개가 형성되며 4.0∼6.6×2.0∼2.2 ㎛이다. 본 균주는 Samson 분류에 의거하여 주요 특성이 일치하여 Paecilomyces tenuipes(Peck) Samson 균과 동일 종으로 인정되었다. Paecilomyces tenuipes (Nunkkot Dongchunghacho) was erroneously treated in three different scientific names: Paecilomyces japonica,k Isaria japonica and Paecilomyces tenuipes. Since the fungus has been widely used in various fields of research and industry, its nomenclatural uncertainty caused many problems. Therefore, morphological studies of korean isolate were carried out and compared with published data on other isolates of P. tenuipes to establish its proper taxonomec position.
권학철(Hak Cheol Kwon),문형인(Hyung In Moon),최상훈(Sang Hoon Choi),이정옥(Jung Ock Lee),조세연(Sae Yun Cho),정이연(I Yeon Jung),김선여(Sun Yeou Kim),이강노(Kang Ro Lee) 대한약학회 1999 약학회지 Vol.43 No.2
The acivity-guided fractionation on the MeOH extract of Bombycis corpus inoculated by Beauberia bassiana 101A led to the isolation of two steroids, 24-ethylcholest-4-ene-3,6-dione (1) and ergosterol peroxide (2), as active principles. Compounds 1 and 2 exhibited cytotoxicity against cultured human tumor cell fines, A-549, SK-OV-3, SK-MEL-2, XF-498 and HCT-15 with ED50 values ranging from 3.42 to 11.37mcg/ml.
동충하초 재배상 내 기형균 및 오염균의 발생현황 및 특성
남성희 ( Nam Seong Hee ),윤철식 ( Yoon Cheol Sik ),정이연 ( Jung I Yeon ),지상덕 ( Ji Sang Duk ),조세연 ( Cho Sae Yun ),한명세 ( Han Myung Sae ) 한국잠사학회 2002 한국잠사곤충학회지 Vol.44 No.1
7개도 22개 재배농가로부터 수집한 시료는 총 529점이었으며, 수집균은 두 가지 유헝으로 구분되며 생육장애에 의한 기형균은 23.2%, 오염균은 50.9%였다. 생육장애는 C-1, C-2, C-3 및 C-4의 4가지 유형이 있으며 이들 분생자병속은 20∼70여 개가 발생되는데 회색, 순백색 또는 등황색을 띤다. 분생자병속 말단은 분지하지 않고 기주 표피에 각각 고유색상을 띠며 PDA 배지상에서 균사생장량은 14일간 43∼75 mm 범위로 눈꽃동충하초균보다 빠른 생장을 보인다. Occurrence and charaberistics of infectious fungi and physiologically abnormal Paecilomyces tenuipes were examined. These unfavorable fungi are recently known to cause yield loss, decreased quality of products in artificial cultivation farms of P. tenuipes. Total 529 fungal isolates were collected from 22 farms of 7 provinces. These isolates were composed of physilolgically abnormal P. genuipes strains and other infectious fungal strains, which constituted 23.2% abd 50.9%,k resoectuvekt. Physiologecally abnormal P. tenuipes strains showed irregular synemata,k and absence of local color. They were divided into 4 type of C-1, C-2, C-3,and C-4. Other infectious fungal strains were idevified to 5 species,Bearveria bassiana, Fusarium sp., P.fumosoroseus, Tricothecium roseum,k Aspergillus parasiticus,k which were 22.1, 13.8, 7.6, 40. and 3.4%, respectively. All of them were hyphomycetous fungi, did not produce synemata, and revealed faster growth rate than that of P. tenuipes.
家蠶으로부터 分離된 새로운 Microsporidia S80의 特性
林鍾聲,趙世衍 COLLEGE OF AGRICULTURE KYUNGPOOK NATIONAL UNIVERSI 1983 慶北大農學誌 Vol.1 No.-
The new microsporidia S80 isolated from, Bombyx mori L. in Korea showed ovoid in the morphology of the spores and the size were measured 2.9±0.28μ in length and 1.7±0.29μ width. No other microsporidian spore like this has not been so far isolated from Silkworm. The length of the polar filament extruded in hydrogen peroxide (H_(2)O_(2)) at 30℃ was 26μ of a round cytoplasm on the top. The spores were partly stained with Giemsa, Safranin-O and Gram as the same staining properties as Nosema bombycis, Microsporidia K 79 and other microsporidian spores. The fine structures were observed under scanning eleceron microscope through ultrathin sectioning. The spore wall was composed of three layers ; the thin exospore of an electron dense rippled layer, the thick electron lucent endospore which was thinning considerably at the polar filament insertion point, and the inner limiting membrane. Polar cap present at the sporeapex, with a long polar filament of 12-13 coils, subtending angle of 60˚ to spore axis, which is tubular made up of a multilayered and are a benes core, light ring structure enclosing the dance core, the dark ring structure enclosing the inner light ring structure and the other than and light ring structure bounded from cytoplasm. Lamellate polaroplast occupied the anterior part of the spore, and the two neclei with dense nucleoplasm bounded by a double nuclear envelope were cited in the slight downer middle portion of spore. From the characteristics of the shape, size and fine structures, it is certain to reason the Microsporidia S80 belong to the phylum Microspora, class Microspora, order Microsporida. order Microsporida. The shape of two nuclei cited seems to be genus Nosema, but in the classification for the suborder it should be defined wheather pansporoblasts be formed or not and for the genis especial attempts have been made to define the characters which distinguish the disporous genera in the life cycle. Survey through the infection of the bad cocoons during 1980 to 1982 in South Korea the areas contaminated with new microsporidia were revealed 5 provinces of Kyung-Gi, Kang-Won, Chung-Nam and Chun-Nam. Pathological effects inoculated per os at second instar larvae of silkworm, the LD 50 was 7.1×10^(7)/ml as lower pathogenecity than that of Nosema bombycis Naegeli of 1.2×10_(7)/ml. While on the other hand the inoculation of the microsporidia at fourth instar larvae lowerd the whole cocoon weight and cocoon shell wegith and significant at 1% level. The microsporidia S80 defined it can not be transmitted transovarially from the result of predictive and collective examination of 21 egg barches from the infected female moth.
남성희,정이연,조세연,한명세 한국잠사학회 2001 한국잠사곤충학회지 Vol.43 No.1
This study was carried out to improve the liquid culture methods of paecilomyces haponica. The results show that the size of granular mycelium is smaller when the shaking speed is increased. Especially, the granular mycelium is the smallest at the shaking speed of 150 rpm under the photoperiod of 12L-l2D. Dry weight of mycelium was averagely 1.216g in the Silkworm larva (SL) medium, and the weight was 2 times heavier than in the Potato dextrose (PD) medium. By adding 6g of 6 mm beads in the SL medium, the dry weight is increased to 1.332g. The optimal addition of silkworm larval powder to the culture medium for best harvest was 1.360±0.67g in dry weight.