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      • KCI등재후보
      • KCI등재후보

        파드득나물 균핵병균(Sclerotinia sclerotiorum)의 균핵 형성 및 생존에 미치는 온도의 영향

        장석원,김성기,이한범 한국식물병리학회 2003 식물병연구 Vol.9 No.1

        Sclerotinia rot of C. japonica caused by Sclerotinia sclerotiorum was observed throught the growing season in the vinylhouse at the Namyangju, Yangju and Yangpyung areas. The disease was commonly occurred at late autumn after transplanting and continuously increased until spring in next year. The disease was especially severe at the Yangpyung area among three areas. Three isolates of S. sclerotiorum obtained from C. japonica in different geographic areas were evaluated for ability of sclerotia formation and viability under different temperature. Temperature for mycelial growth ranged from 5 to 30oC, with optimum temperature at 15~30oC. Sclerotia were fewer formed at low temperature, but their size was larger than that at high temperature. Small size of sclerotia was more viable than those of large size at high temperature. Based on the differences in viability of sclerotia, the relative ranks of sclerotia were in order ~2 mm > 3~4 mm > 5 mm~.

      • KCI등재

        Vegetative Compatibility Grouping of Sclerotinia homoeocarpa Isolates Infecting Turfgrass in South Korea

        장석원,장태현,홍점규,박종현,정석우 한국잔디학회 2011 Weed & Turfgrass Science Vol.25 No.2

        Sclerotinia homoeocarpa, the causal agent of dollar spot, is one of the most common pathogens of cool season turfgrasses in South Korea. The vegetative compatibility group (VCG) assay was carried out using nitratenonutilizing (nit) mutants recovered from 13 South Korean isolates with various geographical origins. The mutants were divided into four phenotypic classes based on mutation loci associated with nitrogen assimilation: nit1, nit2, nit3,and NitM. The recovered number of nit mutants greatly varied among the isolates, ranging from 0 to 15 mutants. Of the mutants isolated, nit1 and nit2 mutants were most common (80%) while NitM and nit3 were relatively rare. One dominant and four minor VCGs were determined from 18 mutant isolates tested. To study population structures of Korean S. homoeocarpa isolates and increase our understanding of its ecological and epidemiological aspects for dollar spot management on turfgrass, more generated mutants should be tested with more diverse isolate collections.

      • KCI등재

        First Report of Sclerotinia Rot Caused by Sclerotinia sclerotiorum on Some Vegetable Crops in Korea

        장석원 한국식물병리학회 2003 Plant Pathology Journal Vol.19 No.2

        Sclerotinia rot occurred severely on some vegetable crops grown in Namyangju, Yangpyung, and Yangju areas in Korea in 2001-2002. The crops infected with Sclerotinia sp. were Adenophora remotiflora, Armoracia lapathifolia, Angelica acutiloba, Angelica archangelica, Anthriscus sylvestris, Aster tataricus, Beta vulgaris var. cicla, Brassica campestris var. marinosa, Brassica juncea var. laciniata, Chichorium intybus, Lactuca indica var. dracoglossa, Lactuca sativa var. oak-leaf, Petroselinum crispum, and Phyteuma japonicum. The fungus associated with the disease was identified as Sclerotinia sclerotiorum, based on the morphological characteristics of the pathogen. The symptoms were water-soaked spots that enlarged later and became a watery soft rot. Infected parts became yellow and then turned brown, followed by death of the whole plant. White mycelia developed on the upper petioles and leaves and on the soil where these plant parts lay. Then black sclerotia in variable size and shape formed from the mycelial mass. Pathogenicity of the fungus was proven by artificially inoculating each crop. This is the first report of Sclerotinia rot on the listed vegetable crops in Korea.

      • KCI등재

        들잔디와 금잔디 수집종의 지역별 적응성 및 봉분 실증 평가

        장석원,성창현,배은지,구준학,윤정호 한국잔디학회 2019 Weed & Turfgrass Science Vol.8 No.3

        Zoysia japonica and Z. matrella are native turfgrass in Korea. Z. japonica is used throughout the country, but Z. matrella is weak in the cold climate and restricted to the southern areas. Z. matrella has a lot of advantages such as fine leaves, high density and excellent texture, so it is important to evaluate the applicability for various purposes. This study was conducted to evaluate the overwintering survival of the Z. japonica 10 and Z. matrella 18 ecotypes collected from across the country in Yangju, Eumseong, and Namhae areas from 2014 to 2015. Each of the two species was also selected and compared with the adaptability as grave covering grass in virtual grave mounds of actual size in Hapcheon, Gyeongnam province from 2017 to 2018. All of Z. japonica ecotypes overwintered in three areas, but 38.9, 83.3, and 88.9% of Z. matrella ecotypes in the Yangju, Eumseong, and Namhae areas survived through winter, respectively. The strip-sodded graves with two species were established in May 2017. In both Z. japonica and Z. matrella graves, ground-coverages were over 90% by Korean thanksgiving day’ mowing (Beolcho), indicating that there was no significant difference between two species in coverage rates. Survival rates of grave-cover plants of Z. japonica and Z. matrella in the following year with over-wintering were 80% and 28%, respectively. Z. japonica suffered damage in the northern slopes of the grave, but Z. matrella plants suffered with great damage in the northern and western slopes. In green-up timing, Z. japonica was faster than Z. matrella, and the slope of the grave proceeded in the order of south>east>west>north. During the winter season, soil temperatures and moisture in the grave remained low in the order of south>east>west>north slopes. 들잔디와 금잔디는 우리나라에서 자생하는 한국잔디이다. 들잔디는 우리나라 전역에서 사용되지만, 내한성이 약한 금잔디는 남부 지방에 제한되어 사용되고 있다. 금잔디는 세엽, 높은 밀도, 우수한 질감 등 많은 장점을 가지고 있어 다양한 용도로서의 가능성을 평가하는 것은 중요하다. 본 연구는 금잔디의 묘지 잔디로서의 활용 가능성을 파악하기 위해 충청도, 경상도, 전라도, 제주도 등지에서 수집한 들잔디 10 계통과 들잔디 18 계통을 3개 지역(경기 양주, 충북 음성, 경남 남해)에서 2014년-2015년 중에 월동 후 생존율과 그린업 시기를 조사하였다. 또한 2017년-2018년에는 금잔디 1계통과 중엽 들잔디 1품종 밀록 을 경남 합천에서 실제 조성한 가상의 봉분에 피복하여 묘지 잔디로서의 적용 가능성을 비교, 평가하였다. 들잔디는 모든 평가 지역에서 월동 후 생존하였으나, 금잔디는 양주, 음성, 남해에서 각각 38.9, 83.3, 88.9%의 개체가 월동하였다. 들잔디와 금잔디 봉분에서는 모두 5월 줄떼 식재 후 9월 추석 전 벌초까지 90% 이상이 피복되어 두 종 사이에는 피복 속도에서 큰 차이를 보이지 않았다. 하지만 들잔디와 금잔디의 월동 후 생존율은 각각 80%와 28%로 큰 차이를 보였다. 들잔디는 봉분의 북쪽 방향에서 동해를 입었으나, 금잔디는 북쪽과 서쪽 방향에서 큰 피해를 입었다. 봉분에서의 그린업은 들잔디가 금잔디보다 빨랐으며, 봉분의 남쪽>동쪽>서쪽>북쪽 방향 순서로 진행되었다. 동계 기간 중 묘지의 토양 온도와 수분은 남쪽>동쪽>서쪽>북쪽 순으로 높게 유지되었다.

      • KCI등재

        Evidence for Genetic Similarity of Vegetative Compatibility Groupings in Sclerotinia homoeocarpa

        장석원,조영기,장태현,정근화 한국식물병리학회 2014 Plant Pathology Journal Vol.30 No.4

        Vegetative compatibility groups (VCGs) are determinedfor many fungi to test for the ability of fungalisolates to undergo heterokaryon formation. In severalfungal plant pathogens, isolates belonging to a VCGhave been shown to share significantly higher geneticsimilarity than those of different VCGs. In this studywe sought to examine the relationship between VCGand genetic similarity of an important cool season turfgrasspathogen, Sclerotinia homoeocarpa. Twenty-twoS. homoeocarpa isolates from the Midwest and EasternUS, which were previously characterized in severalstudies, were all evaluated for VCG using an improvednit mutant assay. These isolates were also genotypedusing 19 microsatellites developed from partial genomesequence of S. homoeocarpa. Additionally, partial sequencesof mitochondrial genes cytochrome oxidase IIand mitochondrial small subunit (mtSSU) rRNA, andthe atp6-rns intergenic spacer, were generated for isolatesfrom each nit mutant VCG to determine if mitochondrialhaplotypes differed among VCGs. Of the 22isolates screened, 15 were amenable to the nit mutantVCG assay and were grouped into six VCGs. The 19microsatellites gave 57 alleles for this set. Unweightedpair group methods with arithmetic mean (UPGMA)tree of binary microsatellite data were used to producea dendrogram of the isolate genotypes based on microsatellitealleles, which showed high genetic similarityof nit mutant VCGs. Analysis of molecular variance of microsatellite data demonstrates that the currentnit mutant VCGs explain the microsatellite genotypicvariation among isolates better than the previous nitmutant VCGs or the conventionally determined VCGs. Mitochondrial sequences were identical among all isolates,suggesting that this marker type may not be informativefor US populations of S. homoeocarpa.

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