황산처리를 이용한 개싸리 종자의 물리적 휴면 타파

이용하,최한,이수광,이정호,이기철 한국자원식물학회 2016 한국자원식물학회지 Vol.29 No.1

Lespedeza species are mainly used for wildlife food and cover and for erosion control. The germination of these species can be enhanced after a fire occurrence in forest, which is known as fire-activated seeds to germinate. While the heat treatment could break seed dormancy of Lespedeza, its germination rate was quite low. We investigated that chemical scarification could promote germination of L. tomentosa. Seeds were soaked in 100% sulfuric acid (H2SO4) for 0, 1, 3, 6, 12, 24, 48, 96, 192, and 384 min, and then washed in distilled water for 24 h. Very few seeds were germinated in control (H2SO4 for 0 min). More than 90% of seeds were germinated in H2SO4 for 24, 48, and 92 min. However, some damage was observed in roots and cotyledons of seedling dipped in H2SO4 for a long time. To search the optimal soaking time in H2SO4 without defects, seeds scarified in H2SO4 for 30, 60, 90, 120, 150, 180, and 300 min were sown the commercial soil medium. Seeds treated with H2SO4 for 90 min and 150 min emerged by about 92% and 84%, respectively. Therefore, H2SO4 treatment could break the seed dormancy of Lespedeza species, and especially in case of L. tomentosa the optimal treatment time in sulfuric acid was one to two hours. Germination of L. tomentosa began promptly following the scarification and was completed within about one month, indicating that seeds has no physiological dormancy, just has physical dormancy. Key words - Fabaceae, Germination, Germination uniformity, Scarification, Seedling emergence 싸리속(Lespedeza)식물은 가축사료, 제방 녹화용 등으로 활용되는 자원식물이다. 이들 종자는 산지의 화재 이후에 발아, 출현하는 fire species로 알려져 있다. 선행연구에서 이런 점을 착안하여 인위적으로 열을 가했을 때 발아가 되었으나 발아율이 낮고 처리가 용이하지 않았다. 따라서 본 연구는 개싸리 종자를 황산처리하여 화학적으로 종피처리하여 발아율을 높이기 위해 수행되었다. 개싸리 종자를 98% 황산에 0, 1, 3, 6, 12, 24, 48, 96, 192, 384분 동안 침지시키고 흐르는 물에 24시간 동안씻었다. 황산처리를 하지 않은 대조구의 경우 거의 발아가 되지않았지만, 황산처리 기간이 길어질수록 개싸리의 발아율이 증가하였다. 황산 24, 48, 92분 처리에서 90% 이상 발아가 되었다. 그러나 장기간 황산처리시 발아된 유근에 황산피해가 관찰되었다. 기외 실험에서 황산처리 후 건전묘 출현을 알아보기 위해 황산 30, 60, 90, 120, 150, 180, 300분을 처리한 원예상토가충진된 트레이에 파종하고 비닐온실에서 발아율을 측정하였다. 황산 90분, 150분 처리에서 발아율은 각각 92%, 84%였으나 이보다 더 긴 기간 동안 처리하였을 때 출현율이 떨어졌다. 따라서개싸리의 발아를 위한 황산 최적시간은 1~2시간으로 판단된다. 개싸리는 종피처리 이후 바로 발아가 시작되어 대부분 1달 이내에 발아가 완료되었으며, 이는 개싸리 종자는 생리적 휴면은 없는 물리적 휴면을 가지고 있음을 알 수 있다.

사과線蟲에 關한 硏究 : 사과線蟲의 根部被害에 關하여

李容夏 慶北大學校 1966 論文集 Vol.10 No.-

The author investigated on the root-damage by apple wooly aphid that damage to the apple tree root seriously at apple production area from June to October and on the tree growth influenced by damage of this insect on the American summer beauty trees in 1965. The results can be summarized as follows; 1. American summer beauty were damaged by apple wooly aphid average 40 percent at the heart land of apple production of Kyungpook province, Chung Nam, Pook and Chun Nam, Pook in Korea. 2. Investigation of tree growth influenced by damage of apple wooly aphid on the 40 American summer beauty trees with age of 30 years old were conducted. Differences were found at the non-damaged plots and seriously damaged plots in the diameter of trunk and also diameter and length of shoot were appeared differently at that of plots.

우리 나라 果樹栽培의 實態에 關한 硏究 : 사과 栽培의 經濟的 實態에 關하여 The studies on the Economic Problems in the Apple orchard Management

李容夏,崔永然 慶北大學校 1965 論文集 Vol.9 No.-

The authors investigated on the economic relation with apple management in Taegu city where is the heart-land of apple cultivation and near several Kun from 1961 to 1964. The results of investigation can be summarized as follows; 1. The vigorous apple trees planted orchards have received 52382 Won of average total income for one year management per 10 a. Agricultural expenditures, 22590 Won were expended. The costs of fertilizer, agricultural chemicals and wages were expended in the same proportion. The net income of orchard manager was 29541 Won out of average total income. 2. When compare with the well managed orchards in Chung-Nam that have relatively many orchard than the other provinces except Taegu city, Kyung-Pook. In net income, Kyung-Pook obtained more income than Chung-Nam. The cost of fertilizer in agricultural expenditures were far much in Chung-Nam while agricultural chemicals, wages and the others were much in Kyung-Pook.

生長調節制에 依한 主要果樹類 花粉의 發芽에 關한 硏究

李容夏,鄭三澤 慶北大學校 1972 論文集 Vol.16 No.-

The authors studied on the pollen germination of apple, pear, and peach according to the dates passed after collection with treatment of G.A., Boric acid, and 12% sugar solution. The results obtained are as follows; 1) Germination percentage of apple was the highest by average 67.6% in the Boric acid plot while lower in the G.A. plot by 2% than control plot. Germination peak was 2 or 3 days after inoculation. Elongation of pollen tube was the shortest time in the Boric acid plot by only one hour for 50 microns in tube length. The more time was required as the dates were passed. 2) Germination percentage of pear was shown higher in the Boric acid plot by average 56.6% than any other plots. Time required for pollen tube elongation was the same tendency as pollen germination. 3) Germination percentage of peach pollen was shown the highest in the G.A. plot by 68.7% rather than other plots on the contrary to the apple and pear varieties. Boric acid and control plots were appeared nearly the same percentage by 47.0% and 45.8% respectively. Particulary, all the pollens were not germinated after two days after storage but elongation of the tube was quicker in the Boric acid plot than other plots. From these results, G.A. has inhibitory action on the pollen germination.

엽비防除에 對한 Folidol의 效果에 關하여

李容夏 慶北大學校 1958 論文集 Vol.2 No.-

A considerable damagable, noxious insect is the Spider Mites during the Apple production of our country. Spider Mites come in great numbers during the year at irregular times. They increase day by day as unrelated medical compound spread. With the great increased noxious insects every year, the only way of exclusion is the rational use of Folidol. The following sundry is the importance of conclusion of his experiment: 1. To fully understand that the nature and characteristics of medicine and the process of Folidol. 2. Use of folidol in the early part of the growing season to kill the insects and spawnin since it is difficult to completely exclude this process after the growth of spider m es and raised up density. 3. Do not raise only density but spray two times continually within the dansity during vigor part of the occurance of spider mites. 4. Apply folidol spray once a week continually since Folidol value grows between 5 and 7 days. 5. Folidol is of good value to the use of other experiments beside spider mites and exculusion of noxious insects so it is good to save any other medicines. 6. There is a great difference in the single method and mixing method when applying the systematic sprey of medicines.

生長調節劑에 依한 사과 開花期 調節에 關한 硏究

李容夏,全在琪,李愚升,金鍾震,郭炳華,鄭三澤,徐榮敎 慶北大學校 生産技術硏究所 1967 生産技術 Vol.2 No.-

사과의 開花期를 促進또는 抑制하기 爲하여 M. H. 와 Auxine H61 을 開花期 20 日前에 撒布하였다. Auxine은 H61 3,000倍~4,000倍를 祝, 紅玉에 撒布해 줌으로서 1~2 日의 開花促進을 얻었고 M. H 1,000~2,000 ppm을 撒布함으로서 1~2日의 開花抑制效果를 얻었다. 低濃의 M.H 處理로서는 效果가 認定되지 않었고 2,000 ppm以上의 處理로서는 藥害가 있는것으로 認定되었다. 處理時期 處理濃度 處理反覆 回數에 對해서는 繼續實驗이 必要할것으로 본다.

연 투여시의 마우스 간 조직내 δ-Aminolevulinate Dehydratase의 명역화학적 변화

이용하,정규철 중앙대학교 의과대학 의과학연구소 1981 中央醫大誌 Vol.6 No.4

Immunochemical alterations, of hepatic δ-aminolevulinate dehydratase from ICR strain of mice exposed tio lead in vitro (10 mM Pb^++) and in vivo (intraperitoneal injection of Pb^++ 1.3mg per 10 grams of body weight) were investigated by Ouchterlony gel double diffusion analysis as described by Kabat and Mayer (1967) and Campbell et al. (1970). and two-dimensional immunoelectrophoresis by Laurell (1965). Rabbit antiserum against hepatic δ-aminolevulinate dehydratase from ICR strain of mice purified 230-folds by methods of Doyle and Schimke (1969) modified by Lee and Chung (1979) was prepared as described by Kenny (1971) and Alexander and Kenny (1973). Heat-treated (at 64℃, for 10 minutes) supernatants (45,000×g for 2 hours and 20 minutes) of various tissue homogenates were used as antigens. Ouchterlony gel double-diffusion analysis revealed that the antigenicity of the δ-aminolevulinate dehydratase was identical between those distributed in various tissues of the mice. Administration of lead in vivo, 3 hours prior to evisceration seemed to affect the antigenicity of the δ-aminolevulinate dehydratase, resulting in the production of an additional precipitation line on the gel diffusion slide. Normal hepatic δ-amiriolevulinate dehydratase from ICR strain of mice showed 3 protein peaks with mobilities of 1.01(peak A), 0. 80(peak C), and 0.43 (peak D) relative to the mobility (1.0) of bovine serum albumin fraction V (peak B). The immunoelectrophoretic pattern was identical with the normal δ-aminolevulinate dehydratase unaffected by the addition of lead in vitro whereas administration of lead in vivo induced an appearance of additional peak E with the relative mobility of 0.68. Three protein peaks that appeared in two-dimensional immunoelectrophoresis were considered as common protein fractions of δ-aminolevulinate dehydratase, and peak E as an unique protein fraction caused by de novo production of lead type δ-aminolevulinate dehydratase by addition of lead in vivo as suggested by Cantrell et al. (1977). These findings, therefore, could be an important. clue to the possibility of developing an immunological diagnostic tools for the evaluation of lead absorption. Further study would be required to determine the dose-response relationship between the amount of lead exposed and appearance and extent of production of the unique protein fraction.

Krukenberg 종양 4예

이용하,강영호 한일병원 1970 한일병원 임상잡지 Vol.1 No.1

연의 체내분포에 관한 실험적 연구

이용하,정규철 중앙대학교 의과대학 의과학연구소 1979 中央醫大誌 Vol.4 No.1

This experimental study was designed to investigate a pattern of lead distribution in various tissues when an individual was exposed to inorganic lead. Sprague Dawley male albino rats weighing 250∼300 g were used for the study. The rats were divided into several groups and 1% lead nitrate solution was administered into abdominal cavity, 0.01, 0.03, 0.05 and 0.07 ml/g of body weight, respectively, to each group of rats. The lead levels in tissues were analyzed consecutively following the administration of the lead by dithizone methods of Keenan et al. Effects of alcohol on lead absorption was also discussed. 1. Lead contents per gram of fresh weight of tissue of normal rats were the highest in spleen and liver, and high in heart and kidney, and the lowest in brain, muscle and blood. The pattern of lead distribution in tissues of rat was similar with that in human. The lead concentrations of normal rats were much higher than those in human tissues. 2. Lead concentrations in spleen and liver increased promptly showing the highest levels 30 minutes or 1 hour following the administration of lead and kept higher levels until 16 hours of observation. The lead concentration in kidney steadily increased until it reached the maximum level at 4 hours after the administration, when the actual lead level per gram of fresh weight of the tissue was much lower than those in liver and spleen. Blood lead concentration reached the highest value 4.35㎍/ml 2 hours following the administration of lead. Even though the actual lead levels in blood is lower than in other tissues, rate of increase was 41 times as high as normal blood levels of rats. 3. It seemed to enhance the rate of lead absorption from the abdominal cavity when the 50% of ethanol was simultaneously administered with lead. 4. As the dosage of lead increased, the rate of lead absorption in each tissue decreased. This suggests that each tissue has its own limitation in the capacity of lead absorption that varies by each tissue.

기초연금제도의 구상과 문제점

이용하 한국사회복지정책학회 2001 사회복지정책 Vol.12 No.-