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PCB ( Aroclor 1254 ) 가 한국산 담수어류에 (淡水魚類) 미치는 영향
윤여표,이성규,김용화,강원식,지연태,이세창 ( Yeo Pyo Yun,Sung Kyu Lee,Yong Hwa Kim,Won Seek Kang,Youn Tae Chi,Se Chang Lee ) 한국하천호수학회 1994 생태와 환경 Vol.27 No.3
The purpose of this study was to elucidate the adverse effects of Aroclor 1254 on domestic freshwater fish. The acute toxicity in terms of LC_(50)(mg/L) in 96 hours was evaluated for the freshwater fish, Oryzias latipes, Cyprinus carpio and Misgurnus anguillicaudatus. 96-hr LC_(50) values(that concentration kills half of the firth in 96 hours) were 0.053mg/L for newly hatched Oryzias latipes but above 200mg/L for adult Oryzias latipes. Concentrations of Aroclor 1254 above 100mg/L were not lethal to Misgurnus anguillicaudatus but half of Cyprinus carpio were dead after 96 hours in 100mg/L concentration. To assess the danger of Aroclor 1254 to aquatic invertebrates, bioassays were conducted with Daphnia magna. Fifty percent of hatched Daphnia magna died at 0.048mg/L concentration after 96 hours. It is suggested from this study that further study on Aroclor 1254 or other PCBs should be focussed en the mortality of the fish larvae with chronic exposure at low concentrations.
6-(3,4-디클로로페닐)아미노-7-클로로-5,8-퀴놀린디온의 항진균작용 및 안전성 평가
윤여표(Yeo pyo Yun),김동현(Dong Hyun Kim),이병무(Byung Mu Lee),허문영(Moon Young Heo),정해문(Hae Moon Chung),강혜영(Hye Young Kang),최정아(Jung Ah Choi),김도희(Do Hee Kim),유충규(Chung Kyu Ryu) 대한약학회 1998 약학회지 Vol.42 No.5
6-(3,4-Dichlorophenyl)amino-7-chloro-5,8-quinolinedione (RCK50) was tested for antifungal activities in mice systemically infected with Candida albicans. The therapeutic potential of RCK50 was also assessed in comparison with ketoconazole. CK50 had ED50 0.22 +/- 0.01mg/kg. Ketoconazole as a positive control had ED50 6.00 +/- 1.70mg/kg. Intraperitoneally administered RCK50 at the ED50 for 7 days and 14 days reduced Candida albicans colony count in the kidneys and liver. And administered RCK50 at the ED50 for 14 days improved survival rates. The genotoxicities of RCK50 had been evaluated. RCK50 was negative in Ames test with Salmonella typhimurium and chromosomal aberration test in CHL cells. RCK50 did not show any clastogenic effect in mouse peripheral blood and was negative in mouse micronucleus assay. These results indicate that RCK50 has no genotoxic potential under these experimental conditions. Acute oral toxicity studies of RCK50 were carried out in ICR mice of both sexes. RCK50 did not show acute oral toxicities and LD50 values were over 2,850mg/kg in ICR mice.
녹차카테킨과 에피갈로카테킨갈레이트의 산화적 스트레스에 대한 억제효과
윤여표(Yeo Pyo Yun),박종범(Jong Bum Park),허문영(Moon Young Heo) 대한약학회 2001 약학회지 Vol.45 No.1
Green tea catechins (GTC) and its major component, (-)-epigallocatechin gallate (EGCG) were studied for their protective effect against reactive oxygen species (ROS)-induced oxidative stress. GTC and EGCG showed the strong antioxidative effects on the lipid peroxidation of ethyl linolate with Fenton's reagent and free radical scavenging effect to DPPH radical generation. They also protected H2O2-or KO2-induced cytotoxicity in CHL cells or mouse splenocytes. These results indicate that GTC and EGCG are acpable of protecting the lipid peroxidation, free radical generation and cytotoxicity induced by ROS. The mechanism of inhibition in ROS-induced cytotoxicity may be due to their antioxidative and free radial scavenging properties. Therefore, GTC and EGCG may be useful chemopreventive agents by protecting the free radical generation which are involved in cancer and aging.
황귀서,이정석,윤여표,Hwang Gwi-Seo,Lee Jung-Suk,Yun Yeo-Pyo 대한예방한의학회 1997 대한예방한의학회지 Vol.1 No.1
In oriental medicine, Artemisiae asiatica (AA) has been used as analgesic, antiinflammatory agent, and coagulatory agent. Furthermore, eupatilin, a kind of flavonoids, is known as the active principle component of AA. This study was undertaken to determine the gastric antiulceration of AA and to elucidate its mechanism. AA showed the inhibitory effect on gastric ulceration induced by EtOH/HCl and aspirin. To elucidate its mechanism, the effect of AA on lipidperoxide level in gastric mucosa, microsome lipidperoxidation, iron -dependent lipidperoxidation, and neutrophil activation were examined. It is suggested that the antiperoxidative and antineutrophil activity of AA play important roles as a possible mechanism. These results suggest that AA might have gastric antiulceration activity due to antilipidperoxdative and antineutrophil activity.
정진영(Jin Young Jung),윤여표(Yeo Pyo Yun),안희열(Hee Yul Ahn) 大韓藥學會 1997 약학회지 Vol.41 No.5
We studied effects of genistein and tyrphostin, inhibitors of tyrosine kinase, on contractions induced by high K+ and norepinephrine in rat aorta. Genistein (10-6~10-4M) and tyrphostin (10-5~10-4M) inhibited high K+ and norepinephrine-induced sustained contractions, respectively in a concentration-dependent manner. High K+ and norepinephrine caused an increase in 45Ca2+ uptake while 10-4M genistein and tyrphostin inhibited the K+ and norepinephrine-increased 45Ca2+ uptake, respectively. These results show that inhibitor of tyrosine kinase blocks the voltage-and receptor-operated Ca2+ channels in rat aorta, respectively.
LLC-PK1 세포에서의 퓨모너신 B1에 의해 유도된 스핑고리피드 대사
유환수(Hwan Soo Yoo),윤여표(Yeo Pyo Yun) 대한약학회 1997 약학회지 Vol.41 No.6
The purpose of this study was to determine the effect of sulfinpyrazone on fumonisin B1-induced elevation of free sphingoid bases in LLC-PK1 cells. Fumonisins are a family of mycotoxins produced by the fungi Fusarium moniliforme which is common contaminant in corn. Fumonisins are also potent inhibiors of sphingosine and sphinganine N-acyltransferases (ceramide synthases), key enzymes in sphingolipid metabolism resulting in the elevation of free sphinganine. The cytosolic concentration of fumonisin B1 was known to be closely proportional to the elevation of free sphinganine in LLC-PK1 cells [Yoo, H.-S., Norred, W.P., Wang, E., Merrill, A.H., Jr., and Riley, R.T. (1992) Toxicol. Appl.Pharmacol. 114. 9-15]. Sulfinpyrazone, an anion transport inhibitor, reduced the elevated level of free sphinganine resulting from fumonisin B1 inhibition of de novo sphingolipid biosynthesis. Fumonisin B1 at a concentration of 20mcM showed approximately 120pmol/106 cells relative to 3-10pmol/106 cells in control cultures, and sulfinpyrazone at a concentration of 200mcM partially reversed the increased level of free sphinganine induced by fumonisin B1 down to normal level after exposure to fumonisin B1 for 8 to 24hr. However, the reduced effect of sulfinpyrazone on the fumonisin B1-induced elevation of intracellular sphinganine was not shown after 24hr. Fumonisin B1 exposure to LLC-PK1 cells for 36 and 48hr showed approximately 74 and 80pmol per 106 cells relative to 82 and 76pmol,respectively, in fumonisin B<1SUB>1 plus sulfinpyrazone-treated cultures. Sulfinpyrazone-induced less elevation of free sphinganine in confluent cells after exposure to fumonisin B1 suggested that either sulfinpyrazone may block the availability of fumonisin B1 to cells or act on the fumonisin B1 interaction with ceramide synthase.
이경은,심관섭,김진화,박성민,이범천,윤여표,표형배,Lee, Kyung-Eun,Sim, Gwan-Sub,Kim, Jin-Hwa,Park, Sung-Min,Lee, Bum-Chun,Yun, Yeo-Pyo,Zhang, Yong He,Pyo, Hyeong-Bae 대한약학회 2004 약학회지 Vol.48 No.6
Whitening effect, which decreases the skin pigmentation, is the one of important targets in cosmetics. This study was investigated the effects of Scirpi rhizoma on ant ioxidation and melanogenesis. S.rhizoma is a rhizome of Scirpus fluviatilis G. a perennial Cyperaceae species of wide occurrence in Asia, Europe, Africa and North America. S.rhizoma shown scavenging activities of free radicals and reactive oxygen species (ROS) with the IC50 of 638${\mu}g/ml$ against 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical and 21.7${\mu}g/ml$ against superoxide radicals in the xanthine/xanthine oxidase system, respectively. S.rhizoma treatment (48 h) suppressed the biosynthesis of melanin up to 27% and reduced tyrosinase activity up to 31% at 100${\mu}g/ml$ in B16 melanoma cells. S.rhizoma was also able to significantly inhibit tyrosinase and TRP-1 expres- sion in protein level. These results suggest that S.rhizoma inhibited melanin biosynthesis by regulating tyrosinase activity and expression in B16 melanoma cells. Therefore S.rhizoma may be useful as new whitening agent due to the antioxidant effect and the inhibitory effect against melanogenesis.
백렴 추출물의 Matrix Metalloproteinase-1 발현 저해 효과
조영호,심관섭,김진희,박성민,이범천,표형배,윤여표,박흠대,Cho , Young-Ho,Sim , Gwan-Sub,Kim , Jin-Hui,Park , Sung-Min,Lee , Bum-Chun,Pyo , Hyeong-Bae,Yun , Yeo-Pyo,Park , Hum-Dai 대한약학회 2004 약학회지 Vol.48 No.6
Matrix metalloproteinases (MMPs) are known to play an important role in photoaging by mediating the degradation of extracellular matrix proteins. In this study, to develop a n ew anti-aging agent, we investigated the antioxidant activity and the inhibitory effect of Melothria heterophylla extract on expression of MMP-1 in UVA-irradiated human dermal fibroblasts and MMP-1 activity. The M.heterophylla extract was found to scavenge radicals and reactive oxygen species (ROS) with the $SC_{50}$ values of $13{\mu}g/ml$ against 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals and $20{\mu}g/ml$ against superoxide radicals in the xanthine/xanthine oxidase system, respectively. UVA-induced MMP-1 expression was reduced about 80% by $100{\mu}g/ml$ of the M.heterophylla extract but MMP-1 Mrna expression was not inhibited. Therefore, we conclude that the M.heterophylla extract significantly inhibits MMP-1 expression at the protein level. Also, the M.heterophylla extract inhibited MMP-1 activity in a dose dependent manner. From these results, we suggest that the M.heterophylla extract can be used as a new anti-aging agent by antioxidant activity, regulation of UVA-induced MMP-1 production, and inhibition of MMP-1 activity.