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      • KCI등재

        영구임대주택에 거주하는 일반청약가구와 생활보호 가구의 가족특성 및 주거계획 비교

        윤세진,조재순 한국주거학회 1995 한국주거학회 논문집 Vol.6 No.1

        The purpose of this research is to find out the differences in family chareteristics and housing plans between public supporting households and general rental households in the public rental housing. One hundred eighty nine residents in chonju were analyzed. There were some differences between the two groups in family characteristics such as family size.age.a job.sex of the household head existence of mate.etc. The most desired facility was a public health center. The desiring macro environment and plan to move were similar between two groups. There were differences in the opinions on housing policies for the public rental housing between two groups.

      • SCOPUSKCI등재
      • SCOPUSKCI등재
      • KCI등재

        Expression of Growth Differentiation Factor-9 in the Mouse Ovaries at Different Developmental Stages

        윤세진,이경아,고정재,차광열 한국발생생물학회 1999 발생과 생식 Vol.3 No.1

        Growth/differentiation factor-9 (GDF-9)은 transforming growth factor (TGF-) superfamily의 member로서 난소의 난자에서만 특이적으로 발현되며 정상적인 난포발달에 있어 필수적인 성숙인자로 최근에 알려졌다. 본 연구는 RT-PCR을 통해 생쥐의 원시난포에서의 GDF-9 mRNA의 발현 여부와 함께 난포의 발달단계에 따른 상대적인 발현량을 분석하고자 실시하였다. 본 실험에는 ICR 생쥐를 Growth differentiation factor-9 (GDF-9) is a member of the transforming growth factor (TGF-) superfamily. It has been known that GDF-9 is a growth factor having a crucial role in normal folliculogenesis and its expression is oocyte-specific. The present study was aimed to elucidate the expression of GDF-9 mRNA in the mouse primordial follicles as well as in the other developmental stages. The semiquantitative analysis of GDF-9 mRNA expression was conducted. Total RNA was extracted from the ICR mice ovaries at gestational day 19, postnatal day 1, day 10, day 21, and day 28, and RT-PCR was performed to measure GDF-9 and -actin mRNA levels. Level of GDF-9 mRNA were normalized against the level of -actin mRNA, and compared among different stages. GDF-9 mRNA was detected in all samples including the fetal ovaries that mainly consists of primordial follicles. The highest level of mRNA was observed in ovaries obtained at day 10 that mainly consists of growing follicles. The present result suggests that GDF-9 may play an important role in the early stage of folliculogenesis.

      • KCI등재

        아이언 클럽 스윙 시 그립 파지 위치에 따른 역학적 분석

        윤세진,설정덕 한국골프학회 2019 골프연구 Vol.13 No.3

        [목적] 본 연구의 목적은 아이언 7번 클럽을 이용한 골프 스윙 시 그립의 파지 위치로 야기된 운동학적 변인과 지면반력 분석을 통하여 스윙 시 그립 파지 위치 변화를 통한 활용성을 제시하고자 한다. [방법] 대학생 골프선수 10명을 대상으로 아이언 7번을 이용하여 정상 그립과 1인치 짧은 그립 파지를 통한 스윙을 수행하였다. 3차원 동작분석기와 지면반력기를 이용하여 자료를 산출하였고, 그립 간의 차이는 대응표본 t-검증을 이용하여 분석하였다. [결과] 소요시간과 클럽헤드 속도는 대부분 차이가 없었다. 신체중심 변위는 X축의 TS에서 short 그립 시 신체중심의 오른쪽 변위가 크게 나타났고, Y축은 모든 이벤트에서 short 그립 시 신체중심의 전방 변위가 크게 나타났으며, Z축은 ADD, TB, DS, FS에서 short 그립 시 신체중심이 변위가 낮게 나타났다. 지면반력은 Fz의 TS에서 짧은 그립 시 오른발에서 수직힘이 크게 나타났다. [결론] 짧은 그립 사용 시 신체중심 변위에서는 차이가 있지만, 소요시간과 클럽헤드 속도에는 차이가 없기 때문에 정확성은 높이고 파워는 감소되지 않는 짧은 그립을 제안한다. [Purpose] The purpose of this study was to analyze the kinematical variables and ground reaction forces caused by the position of the grip during the golf swing. [Method] 10 Golfers of college students performed a swing with normal grip and 1inch short grip using iron 7. 3D-motion analyzer and force platform were used to collect the data, and the difference between the grips was analyzed using the paired t-test. [Result] Swing time and club head speed were no different. The CM of the X axis showed a large rightward direction in short grip at TS. The CM of the Y axis showed a large forward direction in short grip at all events. The CM of the Z axis showed a large downward direction in short grip at ADD, TB, DS and FS. The GRF showed a large vertical force on the right foot in short grip of Fz at TS. [Conclusion] Therefore, although there is a difference in displacement of CM when using a short grip, there is no difference between swing time and club head speed, suggesting a short grip that increases accuracy and does not reduce power.

      • KCI등재

        Differentially Expressed mRNA Profiles between Immature Germinal Vesicle(GV) and Mature Metaphase II(MII) Mouse Oocytes

        윤세진,정형민,차광렬,김남형,이경아 한국발생생물학회 2004 발생과 생식 Vol.8 No.1

        미성숙의 Germinal Vesicle(GV 단계에서 성숙한 Metaphase II(MII) 단계가 되는 난자성숙 과정은 핵과 세포질의 성숙을 통해 이루어지며, 이를 통해 수정과 배 발달을 할 수 있는 능력을 갖게 된다. GV 난자는 prophase I 단계에 arrest 되어 있다가 meiosis 과정을 거쳐 성숙한 MII로 되는데 이를 조절하는 기작에 대해서는 거의 알려져 있지 않다. 따라서 본 연구는 미성숙 난자와 성숙 난자간의 유전자 발현의 차이 Oocyte maturation refers to the process that prophase I arrested germinal vesicle(GV) drives the progression of meiosis to metaphase II(MII) to have the capacity for fertilization and embryo development. To better understand the molecular mechanism(s) involved in oocyte maturation, we identified differentially expressed genes(DEGs) between GV and MII mouse oocytes using a new innovative annealing control primer (ACP) technology. Using 20 ACPs, we successfully cloned 32 DEGs between GV and Mll oocytes, and 26 out of these 32 DEGs were functionally known genes. Four genes including Pscd2 were GV-specific, 10 genes including PKD2 and CSN3 were highly expressed in GV oocytes(GV-selective), and 12 genes including Diva were highly expressed in MII oocytes (MII-selective). Ail of the genes identified in this study were first reported in the oocyte expression using ACP system and especially, we could characterize the existence of PKD-CSW signaling pathwayin the mouse oocytes. Results of the present study would provide insight for studying molecular mechanisms regulating oocyte maturation.

      • KCI등재후보

        체외배양 중인 생쥐 난소에서 초기난포 조절인자의 발현

        윤세진,김기령,정형민,윤태기,차광렬,이경아,Yoon, Se-Jin,Kim, Ki-Ryeong,Chung, Hyung-Min,Yoon, Tae-Ki,Cha, Kwang-Yul,Lee, Kyung-Ah 대한생식의학회 2005 Clinical and Experimental Reproductive Medicine Vol.32 No.3

        Objective: To understand the crucial requirement for the normal early folliculogenesis, we evaluated molecular as well as physiological differences during in vitro ovarian culture. Among the important regulators for follicle development, anti-Mullerian hormone (AMH) and FSH Receptor (FSHR) have been known to be expressed in the cuboidal granulosa cells. Meanwhile, it is known that c-kit is germ cell-specific and GDF-9 is also oocyte-specific regulator. To evaluate the functional requirement for the competence of normal follicular development, we investigated the differential mRNA expression of several factors secreted from granulosa cells and oocytes between in vivo and in vitro developed ovaries. Materials and Methods: Ovaries from ICR neonates (the day of birth) were cultured for 4 days (for primordial to primary transition) or 8 days (for secondary follicle formation) in ${\alpha}$-MEM glutamax supplemented with 3 mg/ml BSA without serum or growth factors. The mRNA levels of the several factors were investigated by quantitative real-time PCR analysis. Freshly isolated 0-, 4-, and 8-day-old ovaries were used as control. Results: The mRNA of AMH and FSHR as granulosa cell factors was highly increased according to the ovarian development in both of 4- and 8-day-old control. However, the mRNA expression was not induced in both of 4- and 8-day in vitro cultured ovaries. The mRNA expression of GDF-9 known to regulate follicle growth as an oocyte factor was different between in vivo and in vitro developed ovaries. In addition, the transcript of GDF-9 was expressed in the primordial follicles of mouse ovaries. The mRNA expression of c-kit was not significantly different during the early folliculogenesis in vitro. Conclusion: This is the first report regarding endogenous AMH and FSHR expression during the early folliculogenesis in vitro. In conclusion, it will be very valuable to evaluate cuboidal granulosa cell factors as functional marker(s) for normal early folliculogenesis in vitro.

      • SCOPUSKCI등재
      • SCOPUSKCI등재

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