카리장석의 유기농 무와 비트 생육촉진효과

심창기,김민정,김용기,홍성준,박종호,한은정,김석철,이승복 한국유기농업학회 2016 韓國有機農業學會誌 Vol.24 No.3

Potassium (K) is an element essential for plant growth. This study was aimed to examine the effects of three formulations of potash feldspar, powder, sand, and granule type on the growth of radish and beet in organic farming. 0.1% three formulations of potash feldspar were treated on the soil before transplanting 15 days-old seedlings of radish and beet in greenhouse. The results showed that all kinds of potash feldspar significantly increased shoot and root growth, and soluble solid contents excepted for root hardness in radish and beet. Among them, the powder type of potash feldspar was the most effective on foliage and root growth of radish and beet. Based on the results, it was confirmed that 0.1% potash feldspar treated in the rhizosphere of radish and beet can promote the growth of them.

Construction of Antibodies for Detection and Diagnosis of Cucumber greenmottle mosaic virus from Watermelon Plants

심창기,이정한,김희규,Sun Min Hong,Ki Soo Han 한국식물병리학회 2006 Plant Pathology Journal Vol.22 No.1

We immunized BALB/c mice with purified Cucumber green mottle mosaic virus isolate HY1 (CGMMV-HY1). Through the selection of positive clones that were grown on the HAT medium, four sensitive monoclonal clones (CG99-01, CG99-02, CG99-03, and CG99-04) were selected from 500 Hypoxanthine-guanine phosphoribosyltransferase positive hybridoma cells. Four sensitive clones of CGMMV-HY1 were determined as IgM type of the subclass of mouse immunoglobulins Ig group. The titer of monoclonal antiserum against CGMMVHY1 was estimated 1:12,800 by the indirect ELISA. Although monoclonal antibodies (MAbs) from CG99-01 and from CG99-04 cross-reacted with Zucchini green mottle mosaic virus and Kyuri green mottle mosaic virus, MAb from the cell line CG99-03 was highly specific to CGMMV. No MAbs cross-reacted with Cucumber mosaic virus-Fny. Only CG99-04 reacted with Pepper mild mottle virus weakly and CG99-02 reacted with both CGMMV and KGMMV. CGMMV was detected from the rind of watermelon fruit by DAS-ELISA of CGMMV-HY1, but not from the flesh of watermelon. Average seed transmission rate of CGMMV in watermelon was 24% from symptomatic watermelon collected from 5 regions of Gyeongnam province. CGMMV was detected by DAS-ELISA with specific MAb of CGMMVHY1 periodically from root stock, during the sequential process for nursery seedling in Haman. Necrotic spots on cotyledons of root stock seedling progressed to reveal the typical symptomatology on the primary leaves of scion upon grafting. Here, we have established MAb based ELISA system, which could accurately detect CGMMV from watermelon seeds, nursery seedlings, transplants and field samples from greenhouse or open out door field as well.

호박 흰가루병, 역병, 푸자리움시들음병에 대한 저항성 품종 선발 및 호박흰가루병 방제를 위한 난황유플러스와 pH교정황토유황합제의 효과 검정

심창기,김용기,변영웅,박종호,한은정,고병구,김민정 경상대학교 농업생명과학연구원 2018 농업생명과학연구 Vol.52 No.5

본 연구는 유기농 호박 재배시 문제가 되는 흰가루병을 효과적으로 방제하기 위한 난황유 플러스와 pH교정황토유황합제의 처리기술을 개발하고자 하였다. 50개 호박 품종을 이용하여 흰가루병, 역병, 시들음병에 대한 병저항성 정도를 검정한 결과, 공시한 대부분의 품종은 흰가루병에 대해 감수성으로 나타났다. 덩굴쪼김병에 대하여 저항성을 보이는 품종은 FR계통의 대목품종 8개였다.역병에 대해서는 대부분 감수성을 나타내었으며, 3개(SP12, SP45, SP48)의 품종만이 중도 저항성을 보였다. 유기농 호박흰가루병을 방제하기 위해서는 0.3% COY(cooking oil and egg yolk mixture)보다 0.3% COY plus (cooking oil and egg yolk mixture plus 0.2% soluble CuSO4)나 1.0% PLS(pH adjusted loess sulfurmixture)를 사용하는 것이 흰가루병의 방제 지속 기간이 길고 효과적이었다. 또한 유기농 호박농가에서 0.3% COY plus나 1.0% PLS를 단독으로 처리할 경우 1주나 2주 간격으로 처리하는 것이 흰가루병 방제에 가장 효과적이며 0.3% COY plus나 1.0% PLS를 조합으로 처리할 경우 3주 간격으로 처리할 경우 흰가루병 방제 효과가 우수하였다. 따라서 0.3% 난황 유플러스(COY plus)나 1.0% pH교정황토유황합제(PLS)를 조합으로 처리할 경우 흰가루병 방제 효과가 우수하여 방제 비용을 절감하여 매우 경제적일 것으로 생각한다. This study was conducted to investigate treatments of cooking oil and egg yolk plus(COY plus) and pH adjusted loess sulfur mixture(PLS) to control effectively powdery mildew in organic squash cultivation. Using 50 varieties including root stock, pumpkin, and squash, the degree of disease resistance to powdery mildew, Phytophthora rot and Fusarium wilt disease was evaluated. Most of the varieties were susceptible to powdery mildew. Eight fusarium resistant varieties showed resistance to Fusarium wilt. Most of them were susceptible to Phytophthora rot, and only three(SP12, SP45, and SP48) varieties showed moderate resistance. The use of 0.3% COY plus or 1.0% PLS was more effective than 0.3% COY in the control of powdery mildew. In addition, 0.3% COY plus or 1.0% PLS treatment at 1-week-interval or 2-weeks-interval was the most effective in the control of powdery mildew. In addition, the combination treatment of 0.3% COY plus and 1.0% PLS at 3-weeks-interval showed a significant effect in powdery mildew control. Therefore, the use of 0.3% COY plus or 1.0% PLS is considered to be very economical because it reduces the cost of control.

종합적 방제기술을 이용한 유기재배 생강의 근경썩음병 억제

심창기,김민정,김용기,홍성준,박종호,한은정,김석철 한국식물병리학회 2015 식물병연구 Vol.21 No.3

본 연구는 유기농 생강재배지에서 발생한 생강 뿌리썩음병을 헤어리벳치와 난각칼슘+훈탄을 혼합 처리하여 방제하고자하였다. 생강의 잎이 황화되고 식물체가 시드는 생강 근경썩음병의 병징은 화학비료 처리구에서 7월 1일부터 시작되었다. 수확기인 10월 2일에 생강 근경썩음병이 더욱 진전되어 생강의줄기가 갈변하고 식물체가 고사하여 36.7-43.0%의 높은 발병율을 보였다. 반면에 헤어리벳치+훈탄+난각칼슘 처리구는 9월말까지 근경썩음병이 전혀 발생되지 않았고 10월 초순까지 발생이 지연되어 1.3-1.7%의 매우 낮은 발병율을 보였다. 토양내Na, Fe, Cu 함량은 헤어리벳치+훈탄+난각칼슘 처리에 의해 처리 전보다 감소하였으나 유기물 함량은 31.6%로 시험 전보다 2 배 증가하였다. 토양내 Pythium sp.의 밀도는 헤어리벳치+훈탄+난각칼슘 처리구(0.3-2.0×103 cfu/g)에서 낮았으나, 화학비료 처리구(12.0-12.3×103 cfu/g)에서 유의적으로 높았다. 따라서, 유기농 생강 재배지에 헤어리벳치를 녹비작물로 재배하고토양 개량제인 훈탄과 난각칼슘을 토양에 처리하면 생강의 고질적인 병인 생강 근경썩음병을 효과적으로 방제할 수 있을 것으로 사료된다. This study was conducted to control ginger rhizome rot treated with the combined treatment, the hairy vetch, carbonized rice husk and eggshell calcium in organic ginger farm. Early symptoms of leaf yellowing and plant wilt began in the chemical fertilizer treatment on July 1. Ginger rhizome rot was more progressed on October 2, and stem browning and dead plant showed a high disease incidence with from 36.7% to 43.0%. On the other hand, the combined treatment did not occur at all until July 1 and delayed the disease incidence to October 2. It showed a low disease incidence of 1.3% to 1.7%. In the combined treatment, the content of soil Na, Fe, Cu was decreased and organic matter was increased twice with 31.6% than previous. Population density of Pythium sp. is lower in the combined treatment (0.3-2.0×103 cfu/g than the chemical fertilizer treatments (12.0- 12.3×103 cfu/g). The combined treatment, hairy vetch, carbonized rice husk and the eggshell calcium is able to control the ginger rhizome rot in organically cultivated ginger field.

Evaluation of Lettuce Germplasm Resistance to Gray Mold Disease for Organic Cultivations

심창기,김민정,김용기,지형진 한국식물병리학회 2014 Plant Pathology Journal Vol.30 No.1

This study was conducted to evaluate the resistance of212 accessions of lettuce germplasm to gray mold diseasecaused by Botrytis cinerea. The lettuce germplasm werecomposed of five species: Lactuca sativa (193 accessions),L. sativa var. longifolia (2 accessions), L. sativavar. crispa (2 accessions), L. saligna (2 accessions), and L. serriola (1 accession); majority of these originated fromKorea, Netherlands, USA, Russia, and Bulgaria. After35 days of spray inoculation with conidial suspension(3×107 conidia/ml) of B. cinerea on the surface oflettuce leaves, tested lettuce germplasm showed severesymptoms of gray mold disease. There were 208susceptible accessions to B. cinerea counted with 100%of disease incidence and four resistant accessions,IT908801, K000598, K000599, and K021055. Twomoderately resistant accessions of L. sativa, K021055and IT908801, showed 20% of disease incidence ofgray mold disease at 45 days after inoculation; and twoaccessions of L. saligna, K000598 and K000599, whichare wild relatives of lettuce germplasm with loose-leaftype, showed complete resistance to B. cinerea. These fouraccessions are candidates for breeding lettuce cultivarsresistant to gray mold disease.

유기종자 법제화에 대한 농업인의 의향 분석

심창기,김민정,고병구,박종호 한국유기농업학회 2019 韓國有機農業學會誌 Vol.27 No.1

The questionnaire survey was conducted on 225 farmers in Gyeonggi-do, Jeollanam-do and Jeollabuk-do. A total of 189 (84%) farmers responded. 72% of the respondents were males, 50.3% were aged 60 or older, and 51.3% had less than 5 years of farming experience. 78.8% of the respondents are pesticide-free, and 44.4% of respondents have less than 0.5 ha of farming scale. 61.4% of the cultivated crops were vegetable crops. The order of seeds and seedlings to buy was tomato (23.3%), cucumber (12.2%) and pepper (10.6%). The cost of purchasing seeds ranged from a minimum of 100,000 won to a maximum of 5 million won. 78.3% of respondents answered that they well-knew or knew about organic seeds. 78.3% of respondents answered that they knew or knew about organic seeds. Of the positive effects of mandatory use of organic seeds, 41.3% of respondents said they would increase confidence in organic certification. However, 41% of respondents who opposed the mandatory use of organic seeds said that “The strengthening of regulations will make organic agriculture more difficult.” When the use of organic seeds is mandatory, 43.4% of the respondents favor direct support for the purchase of organic seeds, which should be supported politically by the state. When organic seeds were supplied, the disease resistant seeds (53.4%) was the preferred characteristic of organic seeds. For the optimal price of organic seeds, 38.6% of respondents wanted the same price as the commercialized conventional seed. In this study, the questionnaire was conducted for three major organic farming regions, but many of the respondents were judged to have a legal position on the mandatory use of organic seeds. Therefore, the results of this study can be used as a basic data for reviewing the legislation on the organic seed production and distribution suitable for the situation of Korean organic farming.

Leaf Rot and Leaf Ring Spot Caused by Rhizoctonia solani in Chinese Cabbage

심창기,김민정,김용기,지형진,홍성준,박종호,한은정,윤종철 한국식물병리학회 2013 식물병연구 Vol.19 No.4

This study was conducted to determine the occurrence of leaf rot and leaf ring spot, caused by Rhizoctoniasolani in Chinese cabbage under seedling nursery and cultivation greenhouses. Symptoms of leaf rot and leafring spot were found in three Chinese cabbage cultivars, Brassica campestris subsp. pekinensis, ‘Ryeokgwang’,‘Daetong’, and ‘CR mat’. In Hwacheon, the disease incidence was 73.8% in the seedling stage of the Chinesecabbage. In Icheon, the symptoms were observed on the upper leaves of the Chinese cabbage cultivar,‘Norangmini’ with 20.5% of disease incidence. The symptoms appeared as primary lesions consisting ofsmall, circular necrotic ring spots with gray color, 1.4−3.0 mm in diameter, accompanied by secondary rotlesions with large irregular borders of leaves. The color of mycelial mat of 20 isolates was dark brown andlight brown. The average hyphal diameter of all the isolates was within 5.01−11.12 μm. Among the 20 strainsisolated from Chinese cabbage, 16 isolates and four isolates anastomosed with the AG-1 (IB) and AG-1 (IC),respectively. Twenty isolates tested were only virulent on foliage parts of Chinese cabbage leaves but wereavirulent on stem parts of the plants. Based on the mycological characteristics and pathogenicity test on hostplants, the fungus was identified as Rhizoctonia solani.

해외 유기농업자재 연구 방향과 국내 연구 현황

심창기 ( Chang Gi Shim ),김민정 ( Min Jung Kim ),이재형 ( Jae Hyung Lee ),박상구 ( Sang Gu Park ) 한국환경농학회 2022 한국환경농학회 학술대회집 Vol.2022 No.-

(10줄 내외) 최근 국내외적으로 코로나사태와 기후변화 등의 어려움에도 불구하고 친환경 유기농업의 재배면적의 증가와 더불어 유기농 식품 및 산업시장이 지속적으로 성장하고 있는 추세이다. 더욱이, 우리나라뿐만 아니라 유럽, 북·남미에서는 지구온도 상승에 대한 농업분야의 대처방안으로 화학농약과 비료 사용량 감축을 위해 유기농업의 확대와 토양탄소격리량 증대를 위한 농법 실천에 주목하고 있다. 이러한 농업여건의 변화속에서 FiBL 스위스연구소를 통해 해외 유기농업자재 연구 방향과 국내 연구 현황을 살펴보고자 하였다. 유럽 유기농업 연구를 대표하고 있는 기관인 FiBL 스위스연구소의 경우 유기농업을 견인하기 위한 유기농업자재와 기술의 개발뿐만 아니라 유기농업인증에 관한 연구도 겸하고 있다. 최근 FiBL 스위스연구소는 기후변화 대응 경운대체, 탄소 및 질소 동태평가, 마이크로바이옴 활용 지속가능한 농업 진단기술 개발, 로봇활용 잡초방제 기술, 유기농식품의 품질향상연구 등에 관한 연구를 진행하고 있다. 국립농업과학원 유기농업과에서는 2022년부터 2026년까지 국내외 농업여건의 변화와 기후변화에 대응하고자 “탄소저감 환경보전형 유기농업과 유기자원 재순환 기술 개발” 연구사업을 추진하고 있으며 “저탄소 실현 유기농업 현장 생산기반 구축 및 기술보급 확대”라는 세부내역사업을 통해 지나치게 수입의존도가 높은 병해충 방제용 유기농업자재 원료 국산화 및 신소재 개발을 추진하고 유기농업의 기본인 유기종자·종묘·종구 등 유기종자 생산기술을 연구하고 보급체계를 구축하는 연구를 추진할 계획이다.

유기농자재인 황토유황합제의 약해 경감 및 흰가루병 방제효과

심창기(Chang-Ki Shim),김민정(Yong-Ki Kim),김용기(Yong-Ki Kim),홍성준(Sung-Jun Hong),김석철(Suk-Chul Kim) 한국농약과학회 2014 농약과학회지 Vol.18 No.4

버섯 폐배지로부터 분리한 방선균 균주 CA-23과 AA-65균주의 키틴 분해능력과 항균력 검정

심창기(Chang-Ki Shim),김민정(Min-Jeong Kim),김용기(Yong-Ki Kim),지형진(Hyeong-Jin Jee),홍성준(Sung-Jun Hong),박종호(Jong-Ho Park),한은정(Eun-Jung Han),김석철(Seuk-Chul Kim) 한국농약과학회 2015 농약과학회지 Vol.19 No.4