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Development of in vitro produced porcine embryos according to serum types as macromolecule
손정민,Don Buddika Oshadi Malaweera,이은송,신상태,조종기 대한수의학회 2013 JOURNAL OF VETERINARY SCIENCE Vol.14 No.3
This study was conducted to establish an in vitro maturation (IVM) system by selection of efficient porcine serum during porcine in vitro production. To investigate the efficient porcine serum (PS), different types of PS [newborn pig serum,prepubertal gilt serum (PGS), estrus sow serum, and pregnancy sow serum] were used to supplement IVM media with or without gonadotrophin (GTH) and development rates of parthenogenetic activation (PA) and in vitro fertilization (IVF) embryos were then compared. The maturation rates of the PGS group was significantly higher when GTH was not added. Additionally, during development of PA embryos without GTH, the PGS group showed significantly higher cleavage and blastocyst formation rates. Moreover, the cleavage rates of IVF embryos were significantly higher in the PGS group, with no significant differences in the blastocyst formation. However, when GTH was supplemented into the IVM media, there were no significant differences among the four groups in the cleavage rates, development rates of the blastocyst, and cell number of the blastocyst after PA and IVF. In conclusion, PGS is an efficient macromolecule in porcine IVM, and GTH supplementation of the IVM media is beneficial when PS is used as macromolecule, regardless of its origin.
내부 연결 그룹이 다른 폴리이미드 상 레이저 유도 그래핀 전극 직접 성장
손정민,황윤찬,박재희,남기호 한국섬유공학회 2023 한국섬유공학회지 Vol.60 No.6
We present a facile direct-writing heteroatom-doped graphene on molecularlycontrolled polyimides using a continuous-wave CO2 infrared laser scriber. We first synthesizedpolyimide (PI) films with various internal linkages such as phenylene, trifluoromethyl,and sulfone groups by conventional two-step polycondensation of 4,4'-oxydianiline withthree different tetracarboxylic dianhydrides. The development of graphene from each PIfilm surfaces was confirmed by characterizing the heteroatom-doped (N-doped, F-doped,and S-doped) laser-induced graphene (LIG) layers. We found that both laser processingparameters (e.g., laser power and scan speed) and chemical backbones of PIs had a largeinfluence on the formation and quality of the LIG, investigated in terms of the ID/IG and I2DRaman bands, crystalline size, surface composition, and sheet resistance. A small amountof heteroatom-doping derived from the PI backbones might easily control the electricalconductivity of LIG electrode. It is predicted that the LIG developed according to theobtained results can offer new opportunities for the required simple, low-cost, and flexible electronics and sensors.
Lumbar Angiomatous Meningioma in a Dog
손정민,김보은,정선영,지서연,윤대영,권오경,김대용,최민철,윤정희 한국임상수의학회 2014 한국임상수의학회지 Vol.31 No.2
An 11-year-old castrated Schnauzer presented with paraplegia for the last two weeks, initially developedpelvic limb ataxia 6 months ago and left pelvic limb paresis 3 months ago. On neurological examination, paraplegiaand postural reaction deficits were noted in the pelvic limbs with no deep pain sensation, and the dog showedexaggerated spinal reflexes and involuntary urination. Magnetic resonance (MR) images of thoracolumbar spinal cordwere obtained with a 0.3 Tesla magnet. A mass with a broad base to the dorsal and left dural margins was identifiedat the level of L2, causing marked spinal cord compression. The mass showed isointensity to the spinal cord on T1-weighted (T1W) precontrast images, hyperintensity on T2-weighted images, marked homogeneous contrast enhancementwith well-defined margins and the “dural tail” sign on T1W postcontrast images. An intradural-extramedullay tumorwas considered, most likely, meningioma was highly suggestive. Left-sided hemilaminectomy and dorsal laminectomywere performed. An intradural mass partially adherent to dura mater was compressing the spinal cord, and the masswas completely removed with the attached dura mater. A histopathologic diagnosis of angiomatous subtype meningiomawas made.