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류성원,김현호,방문남,박영길,박순희,심영수,강성만,배길한,Ryu, Sung-Weon,Kim, Hyun-Ho,Bang, Mun-Nam,Park, Young-Kil,Park, Sue-Nie,Shim, Young-Soo,Kang, Seongman,Bai, Gill-Han 대한결핵및호흡기학회 2004 Tuberculosis and Respiratory Diseases Vol.56 No.3
Background : The resurgence of tuberculosis and outbreaks of multidrug resistant (MDR) tuberculosis have increased the emphasis for the development of new susceptibility testing of the Mycobacterium tuberculosis for the effective treatment and control of the disease. Conventional drug susceptibility testings, such as those using egg-based or agar-based media have some limits, such as the time required and difficulties in determining critical inhibitory concentrations, but these are still being used in many diagnostic laboratories because of no better lternatives, considering cost and accuracy. To overcome these limits, a rapid and simple method for new susceptibility testing, using live and dead assays, was applied for a bacterial cell viability assay to distinguish dead from live bacterial cells based on two-color fluorescence. Materials and Methods Strains : Forty strains were used in this study, 20 susceptible to all antituberculosis drugs and the other 20 resistant to the four first line antituberculosis drugs isoniazid, rifampicin, streptomycin and ethambutol. Antibiotics : The four antibiotics were dissolved in 7H9 broth to make the following solutions: $0.1{\mu}g\;isoniazid(INH)/m{\ell}$, $0.4{\mu}g\;rifampicin(RMP)/m{\ell}$, $4.0{\mu}g\;streptomycin(SM)/m{\ell}$ and $4.0{\mu}g\;ethambutol(EMB)/m{\ell}$. Results : Live and dead Mycobacterium tuberculosis cells fluoresced green and red with the acridin (Syto 9) and propidium treatments, respectively. These results are very well accorded with conventional drug susceptibility testing by proportional method on Lowensen-Jensen media (L-J) containing 4 drugs (INH, RMP, EMB and SM), showing a 93.7 % accordance rate in susceptible strains and 95% in resistant strains. Conclusion : The results of the drug susceptibility testing using the live and dead bacterial cell assay showed high accordance rates compared with the conventional proportion method on L-J. This finding suggests that the live and dead bacterial cell assay can be used as an alternative to conventional drug susceptibility testing for M. tuberculosis strains.
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전자-선 증착 기술에 의해 성막된 다양한 무기 박막들의 투습 방지 특성
류성원,이병로,김화민,Ryu, Sung-Won,Rhee, Byung-Roh,Kim, Hwa-Min 한국진공학회 2008 Applied Science and Convergence Technology Vol.17 No.1
Various diatomic inorganic films and their composite films are packed as passivation films covering Ca cells on glass substrates by using an electron-beam evaporation technique. When these Ca cells are exposed to an ambient atmosphere, the water vapor penetrating through the passivation layers is absorbed in the Ca cells, resulting in a gradual progress of transparency in the Ca cells, which can be represented by changes of the optical transmittance in the visible range. Compared with the saturation times for the Ca cells to become completely transparent in the atmosphere, the protection effects of water vapor are estimated for various passivation films. The composite films consisting silicon oxide($SiO_2$) and tin oxide($SnO_2$) or zinc oxide(ZnO) are found to show a superior protection effect of water vapor as compared with diatomic inorganic films. Also, the main factors affecting the permeation of water vapor through the oxide films are found to be the polarizability and the packing density.
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Activities of the Korean Institute of Tuberculosis
류성원,김희진 질병관리본부 2014 Osong Public Health and Research Persptectives Vol.5 No.-
The Korean National Tuberculosis Association (KNTA) set up the Korean Institute of Tuberculosis (KIT) in 1970 to foster research and technical activities pertaining to tuberculosis (TB). The KNTA/KIT had successfully conducted a countrywide TB prevalence survey from 1965 to 1995 at 5-year intervals. The survey results (decline in TB rates) established Korea as a country that had successfully implemented national control programs for TB. The KIT developed the Korea Tuberculosis Surveillance System and the Laboratory Management Information System, both of which were transferred to the Korea Centers for Disease Control and Prevention after its establishment. The KIT functions as a central and supranational reference TB laboratory for microbiological and epidemiological research and provides training and education for health-care workers and medical practitioners. Recently, the KIT has expanded its activities to countries such as Ethiopia, Laos, and Timor-Leste to support TB control and prevention. The KIT will continue to support research activities and provide technical assistance in diagnosing the infection until it is completely eliminated in Korea.
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류성원,이정수,오재연,김병기,김영,김제형,신철,이승헌 대한결핵및호흡기학회 2018 Tuberculosis and Respiratory Diseases Vol.81 No.3
Background: The number of immigrants with tuberculosis (TB) increases each year in South Korea. Determining the transmission dynamics based on whole genome sequencing (WGS) to cluster the strains has been challenging. Methods: WGS, annotation refinement, and orthology assignment for the GenBank accession number acquisition were performed on two clinical isolates from Chinese immigrants. In addition, the genomes of the two isolates were compared with the genomes of Mycobacterium tuberculosis isolates, from two native Korean and five native Chinese individuals using a phylogenetic topology tree based on the Multiple Alignment of Conserved Genomic Sequence with Rearrangements (Mauve) package. Results: The newly assigned accession numbers for two clinical isolates were CP020381.2 (a Korean-Chinese from Yanbian Province) and CP022014.1 (a Chinese from Shandong Province), respectively. Mauve alignment classified all nine TB isolates into a discriminative collinear set with matched regions. The phylogenetic analysis revealed a rooted phylogenetic tree grouping the nine strains into two lineages: strains from Chinese individuals and strains from Korean individuals. Conclusion: Phylogenetic trees based on the Mauve alignments were supposed to be useful in revealing the dynamics of TB transmission from immigrants in South Korea, which can provide valuable information for scaling up the TB screening policy for immigrants.
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직류 마그네트론 스퍼트링 방법에 의한 투명전도성 ITO 박막의 특성에 미치는 캐소우드 자기장 효과
류성원,김종재,김화민,박승환,홍우표,홍재석 한국물리학회 2006 새물리 Vol.53 No.6
Transparent and conducting In$_2$O$_3$-SnO$_2$ (ITO) thin films were prepared on Corning glass substrates at room temperature by using a DC sputtering method. We investigated the optical and the electrical properties of the ITO films, especially their dependence of the magnetic field applied in the cathode and the target, and examined the optimum condition of the $B$-field to ensure a high-quality ITO film for industrial applications for an in-line sputtering system. % The deposition rate of the ITO films increases, as the intensity of the cathode $B$-field is increased but the resistivity of the films decreases. For magnetic fields larger than 3400 G, the deposition rate saturates and the resistivity finally falls to a constant value of 4.6 $\times 10^{-4}$ $\Omega$cm. If the $B$-field remains constant for a same deposition time, a nearly uniform thickness with a variation smaller than $\pm$ 5 \% is observed around the center of substrate corresponding to the erosion area of the ITO target. The film's thickness becomes thinner as the edges of the substrate are approached. As the cathode field is increased, the film's thickness increases, the sheet resistance of the ITO film decreases, and an oscillating transmittance spectra with multi-peaks occur. The intensity of the $B$-field applied in the cathode should be greater than 3400 G if a high-quality ITO film is to be prepared using in the DC sputtering process. 직류 마그네트론 스퍼터링 방법을 이용하여 상온에서 유리 기판 위에 투명전도성 산화물 ITO (In$_2$O$_3$-SnO$_2$) 박막을 제작하고, 제작된 박막의 광학적 및 전기적 성질을 조사하였다. 본 논문에서는 대량생산에 용이한 직류 스퍼터링 공정에 있어서 고품질의 ITO 박막을 제작하기 위한 중요한 증착조건의 하나로서, 타겟에 걸어주는 자속밀도, 즉 캐소우드 자기장의 세기를 변화시킬 때, ITO 박막 형성 조건과 제작된 박막의 전기적, 광학적 특성들의 변화를 중심으로 논의한다. ITO 타겟에 걸어준 자기장의 세기가 커질수록 막의 증착율은 증가하나 박막의 비저항은 감소하는 경향을 보이며, 3400 G 이상의 자기장에 대해서는 비저항은 한 일정한 값에 접근하게 된다. 증착시간을 동일하게 하고 자기장의 세기를 고정시킬 경우, 대부분의 ITO 박막에서 타겟의 중심 부분에 대응되는 기판 부분에서는 박막의 두께가 거의 균일하게 형성되나 가장자리로 갈수록 두께는 얇아지는 경향을 보인다. 동일한 증착시간에 대해, 자기장이 강해질수록 박막의 두께는 증가하며 면저항은 낮아지게 됨을 알 수 있는데, 두께의 변화에 따라 광학적 투과율 역시 여러 개의 진동하는 스펙트럼을 보이게 된다. 이로부터, 직류 스퍼터링 공정에 있어서 양질의 ITO 박막을 얻기 위해서는 적어도 3400 G 이상의 자기장을 캐소우드에 걸어 줌이 바람직한 것으로 판단된다.
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