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오늘 본 자료
김희선,설혜원,안희진,오선경,구승엽,김석현,최영민,김정구,문신용,Kim, Hee-Sun,Seol, Hye-Won,Ahn, Hee-Jin,Oh, Sun-Kyung,Ku, Seung-Yup,Kim, Seok-Hyun,Choi, Young-Min,Kim, Jung-Gu,Moon, Shin-Yong 대한생식의학회 2004 Clinical and Experimental Reproductive Medicine Vol.31 No.4
Objective: This study was performed to evaluate the possibility of prolonged culture of human embryonic stem cells (hESC; SNUhES2) on human amniotic fluid cells (hAFC), which had been storaged after karyotyping. Method: The hAFC was prepared for feeder layer in the presence of Chang's medium and STO medium (90% DMEM, 10% FBS) at $37^{circ}C$ in a 5% $CO_2$ in air atmosphere. Prior to use as a feeder layer, hAFC was mitotically inactivated by mitomycin C. The hESCs on hAFC were passaged mechanically every seven days with ES culture medium (80% DMEM/F12, 20% SR, bFGF). Results: The hAFC feeder layer support the growth of undifferentiated state of SNUhES2 for at least 59 passages thus far. SNUhES2 colonies on hAFC feeder appeared slightly angular and flatter shape as compared with circular and thicker colonies observed with STO feeder layer and showed higher level with complete undifferentiation in seven days. Like hESC cultured on STO feeders, SNUhES2 grown on hAFC expressed normal karyotype, positive for alkaline phosphatase activity, high telomerase activity, Oct-4, SSEA-3, SSEA-4, Tra-1-60 and Tra-1-81 and formed embryoid bodies (EBs). Conclusion: The hAFC supports undifferentiated growth of hESC. Therefore, these results may help to provide a clinically practicable method for expansion of hESC for cell therapies.
하리자돈(下痢子豚) 분변(糞便)에서 Rotavirus 분리(分離) 동정(同定)
김희선,최정옥,김두희,유영수,안수환,Kim, Hee-sun,Choi, Chung-ok,Kim, Doo-hee,Lyoo, Young-soo,An, Soo-hwan 대한수의학회 1988 大韓獸醫學會誌 Vol.28 No.1
Twenty-eight porcine rotavirus were isolated from piglets with diarrhoea in chonnam province. According to the age, 41 to 60 day old pigs showed the highest isolation frequency among the post weaning pigs. The characteristics of the field isolates were determined by electronmicroscopy(EM), immunofluorescent assay(FA), and electrophoretic migration patterns of the genome profiles. Some of the isolates showed remarkable haemagglutination activity against rabbit and dog erythrocytes, ranged from 4 to 2848, respectively. At least 3 serotypes of porcine rotavirus were recognized by serum neutralization test using serotype specific rotavirus hyperimmune sera.
환경 방사선 생물학적 감시 지표로서 야생 등줄쥐의 방사선 감수성
김희선,니시무라 요시카즈,김종순,Kim, Hee-Sun,Nishimura, Y.,Kim, Chong-Soon 한국방사성폐기물학회 2006 방사성폐기물학회지 Vol.4 No.1
This study examined the possibility of using dark-striped field mice as a biological indicator for the environmental radio-surveillance. For this study, dark-striped field mice were caught from five areas of Kyonggi, Kyongsang, Chungchong and Cholla provinces. The external morphological characteristics and isoenzymic types of dark-striped field mice were studied after they were captured. Among the external morphological characteristics, the dark-brown coat, dark back stripe, head-to-tail length, tail length, and ear length matched the taxonomical characteristics of dark-striped field mice. The analyses on L-lactate dehydrogenase, aspartate aminotransferase, and malate dehydrogenese revealed that one species of dark-striped field mice, called Apodemus agrarius, was inhabitated throughout a wide range of Korea. On the other hand, the frequency of micronuclei in peripheral polychromatic erythrocytes to survived mice after irradiation also analyzed. The LD50/30 of A. cgrarius and ICR mice were approximately 5 Gy and 7.9Gy, respectively. The results of the study reveal that wild A. asrarius have a high potential as a biological monitoring system to determine the impact of radiation in areas such as those within the vicinity of nuclear power plants.
Subfertile 정자처리에 Modified Mini-Percoll 방법의 효용성에 관한 연구
김희선,장현정,이희옥,이숙현,이상훈,Kim, Hee-Sun,Chang, Hyun-Jung,Lee, Hee-Ok,Lee, Sook-Hyun,Lee, Sang-Hoon 대한생식의학회 1994 Clinical and Experimental Reproductive Medicine Vol.21 No.3
Semen preparation for Assisted Reproductive Technology(ART) involves the separation of the motile sperm from non-motile, dead sperm, cellular debris and seminal fluid. The aim of this study is to compare the motile sperm recovery rate and motility index of different sperm preparation method(swim-up method, 80% isotonic continuous percoll method, two-layer discontinuous percoll method, mini-percoll method). In Mini-Percoll method, pellets from patients were suspended in 0.3ml of medium and layerd on a discontinuous percoll gradient consisting of 0.3ml each of 50, 70, 95% isotonic percoll. All semen samples are divided into normal and subnormal sperm group(oligo-, astheno-, oligoasthenozoospermia). Especially, we evaluated the effect of mini-percoll method in subnormal sperm group. In normal sperm group, mini-percoll method and two layer discontinuous percoll method (40%/80%) allowed increasing of motile sperm recovery rate. But motility index was higher in swimup method than the other methods. In subnormal sperm group, mini-percoll method has advantages as compare with the other methods in motile sperm recovery rate and motility index. These results suggest that modified mini-percoll method could be certainly a valuable tool in some cases of severe male factor sperm.
국내 야생따쥐(Crocidura lasiura) 의 Hantavirus 항체 보유실태 및 원인체 분리
김희선,강문일,Kim, Hee-sun,Kang, Mun-il 대한수의학회 1994 大韓獸醫學會誌 Vol.34 No.1
Eleven shrews were caught from three areas of Korea. All of them were confirmed in the same species, Crocidura lasiura. All of sera from wild shrews were examined by indirect immunofluorescent test against Hantaan-related virus. The antibody to Hantaan-related virus was detected by 2 out of 11 shrews. Just 2 of 7 shrews from BG area were sero-positive for Hantaan-related virus antigen and none from other. All of sero-positive for Hantaan-related virus antigen belonged to male with antibody titer of 1:40 to 1:80. Two Hantaan-related viruses were isolated in vivo and in vitro.
의사결정나무 분석을 이용한 이상지질혈증 유병자의 지질관리 취약군 예측: 2019-2021년도 국민건강영양조사 자료
김희선,정석희,Hee Sun Kim,Seok Hee Jeong 한국기초간호학회 2023 Journal of korean biological nursing science Vol.25 No.2
Purpose: The aim of this study was to assess lipid levels and to identify groups with poor lipid control group among patients with dyslipidemia. Methods: Data from 1,399 Korean patients with dyslipidemia older than 20 years were extracted from the Korea National Health and Nutrition Examination Survey. Complex sample analysis and decision-tree analysis were conducted with using SPSS for Windows version 27.0. Results: The mean levels of total cholesterol (TC), triglyceride (TG), low density lipoprotein-cholesterol (LDL-C), and high density lipoprotein cholesterol were 211.38±1.15 mg/dL, 306.61±1.15 mg/dL, 118.48±1.08 mg/dL, and 42.39±1.15 mg/dL, respectively. About 61% of participants showed abnormal lipid control. Poor glycemic control groups (TC ≥ 200 mg/dL or TG ≥ 150 mg/dL or LDL-C ≥ 130 mg/dL) were identified through seven different pathways via decision-tree analysis. Poor lipid control groups were categorized based on patients' characteristics such as gender, age, education, dyslipidemia medication adherence, perception of dyslipidemia, diagnosis of myocardial infarction or angina, diabetes mellitus, perceived health status, relative hand grip strength, hemoglobin A1c, aerobic exercise per week, and walking days per week. Dyslipidemia medication adherence was the most significant predictor of poor lipid control. Conclusion: The findings demonstrated characteristics that are predictive of poor lipid control and can be used to detect poor lipid control in patients with dyslipidemia.
전단경간비 및 축력에 따른 깊은 보의 거동 특성에 대한 연구
김희선,박민혜,이민섭,신영수,Kim, Hee-Sun,Park, Min-Hye,Lee, Min-Seob,Shin, Yeong-Soo 대한건축학회 2011 대한건축학회논문집 Vol.27 No.6
The purpose of this study is to investigate effect of axial loads on deep RC beams. Toward this goal, deep RC beams having different shear span-to-depth ratios are fabricated and bending tests are performed on the beams applied with different magnitudes of axial loads. From the tests, strains on various locations, beam deflections, beam compression and shear strains are obtained. The experimental results show that, for the most of the tested beams, failure due to shear is occurred in the axially loaded deep RC beam and the axial load works beneficially to structural performance. However, when the shear span-to-depth ratio is sufficiently small and the large axial load is applied, the beam is failed earlier than as expected due to bearing failure.
국내 야생 집쥐(Rattus norvegicus)의 murine typhus 감염조사
김희선,강문일,고홍범,Kim, Hee-sun,Graham, Robert Ross,Kang, Mun-il,Koh, Hong-bum 대한수의학회 1994 大韓獸醫學會誌 Vol.34 No.4
Sixty eight wild rats, Rattus norvegicus, were caught from Seoul city, Kyonggi, Kangwon, Cholla, and Kyongsang-provinces. All sera collected from rats were examined by immunofluorescent test to find antibody against murine typhus. The antibody prevalance to murine typhus was 4.4%(3/68). Sero-positive rate was 12.5%(2/16) in Kyonggi province and 12.5%(1/8) in Cholla province. The sero-positive rate difference between sexes of rats was not recognized. Three Rattus norvegicus having antibody to murine typhus were morphologically classified as subadult with 8.3%(1/12), middle-aged adult with 5.9%(1/17), and old adult with 4.8%(1/21), respectively.