Prostaglandin A₂-induced Apoptosis is Not Inhibited by Heme Oygenase-1 in U2OS Cells

Kyoung-Won Ko(고경원),Sun-Young Lee(이선영),Ji-Hyun Ahn(안지현),Jaetaek Kim(김재택),In-Kyung Kim(김인경),Ho-Shik Kim(김호식) 한국생명과학회 2008 생명과학회지 Vol.18 No.11

Prostaglandin A₂ (PGA₂)는 사람 골육종 세포인 U2OS 세포주에서 apoptosis와 heme oxygenase (HO)-1의 발현을 함께 유도하였다. PGA₂에 의한 apoptosis는 HO-1의 과도한 발현이나 HO-1에 대한 small interfering RNA에 의한 발현저하에 의하여 변동되지 않았으나 H₂O₂에 의한 세포사망은 HO-1의 발현 수준에 반비례하여 변동되었다. 또한 thiol antioxidant인 N-acetyl-L-cysteine (NAC)은 PGA₂에 의한 세포사망과 HO-1의 발현 증가를 모두 차단하였지만, non-thiol antioxidant인 butylated hydroxyanisole (BHA)과 ascorbic acid는 세포사망과 HO-1의 발현 유도를 차단하지 않았다. 이와 같은 결과들은 PGA₂는 산화성 손상에 의해서가 아니라 PGA₂의 thiol-reactivity에 의하여 apoptosis와 HO-1의 발현을 유도하며, HO-1의 발현은 PGA₂에 의한 apoptosis와는 독립적인 현상이거나 기능적으로 apoptosis 유도의 하부에 위치하고 apoptosis의 진행에는 기여하지 않을 것이라는 것을 시사해 준다. Prostaglandin A₂ (PGA₂), one of cyclopentenone PGs, induced both apoptosis and heme oxygenase (HO)-1 expression in U2OS cells. PGA₂-induced apoptosis was not perturbed by either over-expression or knock-down of HO-1, whereas H₂O₂-induced cell death was inversely modulated by the expression level of HO-1. In addition, N-acetyl-L-cysteine (NAC), a thiol antioxidant, blocked both apoptosis and HO-1 expression induced by PGA₂. But, non-thiol antioxidants like butylated hydorxyanisole (BHA) and ascorbic acid did not block either apoptosis or HO-1-induction. Taken together, these results suggest that PGA₂ induces both apoptosis and HO-1 expression, which are critically related to the thiol-reactivity of PGA₂, but not oxidative stress, and HO-1 expression may be independent or functionally located downstream of apoptosis by PGA₂ without contribution to apoptosis progression.

한국산 거미의 종 목록(2015년도 개정)

김주필(Joo Pil Kim),예성훈(Seong Hun Ye),이준기(Jun Gi Lee),이준호(Jun Ho Lee) 한국거미연구소 2015 한국거미 Vol.31 No.1

한국산 거미 목록을 최근 분류체계와 새롭게 추가된 분류군을 총망라하여 재정리하였다. 그 결과, 한국산 거미류의 수는 총 48과 281속 795종으로 조사되었으며, 이전 김과 김(2010)의 발표보다 2과 16속 69종이 많았다. 이전의 김과 김(2010)의 목록에서 이용된 분류체계와 비교해 크게 변동된 사항으로는 기존의 코리나거미과(Family Corinnidae)에 속하는 3개 속(Genus Cetonana 괴물거미속(개칭), Genus Paratrachelas 어리괭이거미속, Genus Trachelas 괭이거미속)과 2개 속(Genus Orthobula 십자삼지거미속, Genus Phrurolithus 도사거미속)이 각각 괭이거미과(Family Trachelidae)(신칭)와 도사거미과 (Family Phrurolithidae)(신칭)로 분리, 신설되었으며(Ramírez, 2014), 밭고랑거미과(Family Liocranidae)의 족제비거미속(Genus Itatsina)이 미투기거미과(Family Miturgidae)의 1속(Genus Prochora)에 하위 동종이명(synonymy)으로 편입됨(Bosselaers & Jocqué, 2013)에 따라 Prochora 속의 국명이 족제비거미속으로 개칭되었다. 또한 오소리거미과(Family Zoridae)가 미투기거미과(Family Miturgidae)에 하위 동종이명으로 편입되었으며(Ramírez, 2014), 기존에 미투기거미과에 속해 있던 1속(Genus Cheiracanthium 어리염낭거미속)이 미투기거미과로부터 분리, 신설된 장다리염낭거미과(Family Eutichuridae)(신칭)로 분류되었다(Ramírez, 2014). 그 밖에 속, 종의 단위에서 분류군 신설, 동종이명, 오동정 등의 분류 체계 변동 사항이 있었으며, 이를 본 목록에서 확인할 수 있다. 더불어 근래에 추가된 2종의 무자격명(Nomen nudum) 처리된 종과, 국립공원 생물종 목록집(2012)에 기재되었으나 논문으로 정식 발표되지 않은 미기록종 22종을 별도로 기술하였다. 한국산 거미류는 접시거미과(101종), 꼬마거미과(84종), 왕거미과(79종), 깡충거미과(73종), 그리고 늑대거미과(57종) 이하 순으로 종수가 많았으며, 위의 5과가 전체 종수의 약 50%(394종)을 차지하였다. 전체 795종 중 한국산 고유종은 173종(21.8%)이었고, 일본산 거미류와 512종(64.4%), 중국산과 497종(62.5%), 러시아산과 360종(45.3%), 대만산과 103종(13.1%) 동일종이었다. 또한, 구북구산(Palearctic region)이 80종(10%), 전북구산(Holarctic region)이 37종(4.7%), 그리고 세계공통종(Cosmopolitan species)이 13종(1.6%)으로 분석되었다. A revised new-version of the check list of Korean spiders, including recent systematic systems and new taxa, is presented in this paper. According to additional references in the last 5 years, a total of 795 species of 281 genera belonging to 48 families were checked in Korean spider fauna, and there are 69 species of 16 genera belonging to 2 families more than the previous check list(Kim & Kim, 2010). Typical examples of some changes in systematic systems of Korean spiders compared with the previous check list(Kim & Kim, 2010) are as follows : Two families, the family Trachelidae and the family Phrurolithidae, previously in the family Corinnidae are raised to family status(by Ramírez, 2014). In Korea, the family Trachelidae includes 3 genera(Gen. Cetonana, Gen. Paratrachelas, Gen. Trachelas) and the family Phrurolithidae includes 2 genera(Gen. Orthobula, Gen. Phrurolithus); The family Eutichuridae is raised to family status, which was previously in the family Miturgidae(by Ramírez, 2014). There is one genus(Gen. Cheiracanthium) in the family Eutichuridae in Korea; The family Zoridae is included as a junior synonym of the family Miturgidae(by Ramírez, 2014); The genus Itatsina is included as a junior synonym of the genus Prochora in the family Miturgidae(Bosselaers & Jocqué, 2013). One Pisaurid species(Dolomedes jirisanensis) and a Sparassid genus(Gen. Joopilia, Joopilia joopilis) are nomina nuda. Additionally, There are 22 unrecorded species, which was found by Korean National Park Research Institute(2012) but was not described as papers, check list of them is presented separately from the check list of Korean spiders in this paper. Linyphiidae(101 sp.), Theridiidae(84 sp.), Araneidae(79 sp.), Salticidae(73 sp.), and Lycosidae(57 sp.) spiders are the most in the Korean spider fauna, and species in that 5 families formed about 50%(394 sp.) of the whole Korean spiders. In zoogeographical distribution, Korean spider fauna was found containing 80 Pa, 37 Holarctic, 13 Co, and 173 Korean endemic species. And comparison with faunas of neighboring countries, 512 species of Korean spiders also live in Japan(64.4%), 497 species live in C(62.5%), 360 species live in Russia(45.3%) and 103 species live in Taiwan(13.1%).

허준의 「 동의보감 」 연구

김호 ( Ho Kim ) 한국과학사학회 1994 한국과학사학회지 Vol.16 No.1

서부 경남지역 토마토 농장에서 분리된 Staphylococcus aureus의 내독소 유전자의 특성과 항생제 감수성

김진수(Jin-Soo Kim),이진하(Jin-Ha Lee),김지훈(Ji-Hun Kim),최주미(Ju-Mi Choi),김세리(Se-Ri Kim),하상도(Sang-Do Ha),김근성(Keun-Sung Kim),이규호(Kyu-Ho Lee),김민곤(Min-Gon Kim),김광엽(Kwang-Yup Kim),김철호(Cheol-Ho Kim),정덕화(Duck-Hwa Ch 한국식품과학회 2006 한국식품과학회지 Vol.38 No.2

조직검사 분야에 대한 고찰

김쌍용,김영호 대구보건대학 1994 대구보건대학 論文集 Vol.14 No.-

Malignant tumors depart from the parent tissue morphologically and functionally, and revealed anaplasia or cellular atypia on histological observation. Cytologic evidence of anaplasia includes variation in the size and shape of cells and nuclei(pleomorphism), enlarged and hyperchromatic nuclei with coarsely clumped chromatin and prominent nucleoli, atypical mitoses, and bizarre cells, including tumor giant cells. The distinction between benign and malignant tumor and accurate diagnosis of cancer are, from a practical point of view, the most important diagnostic challenge faced by the pathologist. Histologic and cytologic diagnosis are most useful methods in diagnosis of malignant tumors. In most cases, proper biopsy site, prompt fixation, careful processing and conservative interpretation are required before and accurate diagnosis is secure. Also diagnostic skill and experience of the pathologist are requisited for correct diagnosis. Electron microscopy has proved of significant value in the diagnosis of poorly differentiated tumors, which classification is difficult by routine light microscopy. In metastatic disease that was not preceded by a clinically diagnosed primary tumor, the site of origin is often not readily apparent from the morphologic characteristics of the tumor. In such cases electron microscopic examination and the demonstration of a specific tumor marker may establish the correct diagnosis. Recently several newly developed special diagnostic methods, using biopsy sample such as histochemistry, in situ hybridization, flow cytometry and image analysis are applied in tumor diagnosis and clarify the pathogenetic mechanism of cancers.

안테나 소형화를 위한 Co₂ 페라이트의 마이크로파 특성

김재식(Jae-Sik Kim),최의선(Eui-Sun Choi),김강(Kang Kim),이영희(Young-Hie Lee),송성호(Sung-Ho Song),안종복(Jong-Bok Ahn),김병환(Byung-Hwan Kim),최지영(Ji-Young Choi) 대한전기학회 2011 전기학회논문지 Vol.60 No.12

The sintering behavior and microwave properties of ferrite (Ba3Co2-2xZn2xFe24O41 ceramics) were investigated for microwave applications. Also PIFA type antenna with ferrite was simulated. All samples were prepared by the solid state reaction method and sintered at 1350℃. All ceramics had relatively density above of 92% compare with theoretical density of Ba₃Co₂Fe24O41 ceramics. From the XRD pattens, the Z-type phase was existed as main phase in Ba₃Co2-2xZn2xFe24O41 ceramics. The permittivity and permeability of Ba₃Co2-2xZn2xFe24O41 ceramics were increased with Zn additions and decreased rapidly over frequency of 200~600 ㎒. Several PIFA type antennas with ferrite and FR4 were simulated. All antenna structure had return loss of less than -10 ㏈ at each resonant frequency. Simulated antenna using both ferrite and FR4 showed size reduction of 25% without a significant decrement of efficiency.

증례 : 호흡기 ; 한랭글로불린혈증에서 동반된 기질화 폐렴 1예

김연재 ( Yeon Jae Kim ),김상헌 ( Sang Heon Kim ),김태형 ( Tae Hyung Kim ),윤호주 ( Ho Joo Yun ),신동호 ( Dong Ho Shin ),박성수 ( Sung Soo Park ),손장원 ( Jang Won Sohn ) 대한내과학회 2009 대한내과학회지 Vol.77 No.5S

OPC는 매우 드문 질환으로 저자 등은 혼합한랭글로불린 혈증을 진단받은 환자에게서 감염의 증거가 없고 항생제에 반응하지 않으며 호전과 악화를 반복하는 지속적인 폐침윤을 확인하고 조직 검사를 통해 OPC 진단하여 스테로이드치료 후 호전된 1예를 경험하였기에 보고하는 바이다. Cryoglobulinemia refers to the presence of serum polyclonal immunoglobulins that precipitate reversibly at cold temperature. Cryoglobulinemia can involve various organs, including the skin, liver, kidney, and lung. Although many diseases can lead to organizing pneumonia, reports of organizing pneumonia with cryoglobulinemia (OPC) are rare. We treated a case of prolonged migrating patchy lung infiltration combined with mixed cryoglobulinemia. A 75-year-old man was admitted with progressive dyspnea and cough. The serological, clinical, and pathological findings confirmed the presence of mixed cryoglobulinemia. A chest radiograph and computed tomography (CT) showed multiple patchy consolidations that did not resolve with empirical antibiotic treatment. Additionally, his symptoms did not respond to antibiotics. The transbronchial lung biopsy showed organizing pneumonia, so steroid treatment was started, and the clinical symptoms and radiological findings improved. (Korean J Med 77:S1183-S1187, 2009)

고온 (750 ~ 850℃) SOFC용 밀봉재의 특성에 미치는 고열팽창계수를 갖는 필러의 영향

김빛남,이미재,황종희,임태영,김진호,황해진,김일원,정운진,Kim, Bit Nam,Lee, Mi Jai,Hwang, Jong Hee,Lim, Tae Young,Kim, Jin Ho,Hwang, Hae Jin,Kim, Il Won,Chung, Woon Jin 한국세라믹학회 2013 한국세라믹학회지 Vol.50 No.6

In this study, we report that effects of a filler with a high coefficient of thermal expansion on a sealant for high-temperature ($750{\sim}850^{\circ}C$) SOFC. We designed a $SiO_2-BaO-ZnO-B_2O_3-Al_2O_3$ glass system with a softening temperature higher than $750^{\circ}C$. The properties of the glass system show not only low volumetric shrinking but also low swelling. The glass system did not create a crystal phase during along-term heat treatment. We fabricated a seal gasket with 0, 10, 15, and 20 wt% cristobalite added as filler materials with glass powder. The coefficient of thermal expansion of the seal gasket increased according to cristobalite content. During along-term heat treatment, the leak rate decreased by about 5% after a heat treatment in an oxidizing atmosphere at $750^{\circ}C$ for 2000 h, also decreasing by about 6% after a heat treatment in a reducing atmosphere at $750^{\circ}C$ for 1000 h.

홀 타입 전극을 이용한 전기 수력학 프린팅 기술 개발

김호(Ho Kim),이승미(Seungmi Lee),정재원(Jaewon Chung) 대한기계학회 2012 대한기계학회 춘추학술대회 Vol.2012 No.5

사인파를 이용한 고유 주파수에서의 전기수력학(EHD) 분사

김호(Ho Kim),양중근(JeungKun Yang),정재원(Jaewon Chung) 대한기계학회 2013 대한기계학회 춘추학술대회 Vol.2013 No.5