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Molecular and Cellular Studies of Seed Storage Proteins from Rice and Wheat
김우택 한국농화학회 1989 Applied Biological Chemistry (Appl Biol Chem) Vol.32 No.1
Near full length cDNA clones encoding the rice seed storage protein, prolamine, were isolated and divided into two homology classes based on cross-hybridization and DNA sequencing analysis. These cDNA clones contain a single open reading frame encoding a putative rice prolamine precursor(M.W.=17,200) possessing atypical 14 amino acid signal peptide. Clones of these two homology classes diverge mainly by insertions/deletions of short nucleotide stretches and point mutations. The deduced primary structures of both types of prolamine polypeptides are devoid of any major tandem repetitive sequences, a feature prevalent in other cereal prolamines. No significant homology teas detected between the rice prolamine and other cereal prolamines, indicating that the rice gene evolved from a different ancestor that gave rise to other cereal prolamine genes. Developing wheat and: rice endosperms were examined using ultrathin sections prepared from tissues harvested at various days after flowering. By immunocytochemical localization techniques, wheat prolamines are localized within vesicles from Golgi apparatus and in homogeneous regions of protein bodies. The involvement of the goli apparatus in the packaging of wheat prolamines into protein bodies indicates a pathway which differs from the mode of other cereal prolamines and resembles the mechanism employed for the storage of rice glutelin and legume globulins.
수직력하에서 임프란트 나사형태에 따른 응력의 3차원 유한요소법적 분석
김우택,차용두,오세종,박상수,김현우,박양호,박준우,이건주,Kim, Woo-Taek,Cha, Yong-Doo,Oh, Se-Jong,Park, Sang-Soo,Kim, Hyun-Woo,Park, Yang-Ho,Park, Jun-Woo,Rhee, Gun-Joo 대한구강악안면외과학회 2001 대한구강악안면외과학회지 Vol.27 No.2
There are three designs of thread form in screw type implants: V-thread, Reverse buttress thread and Square thread. The purpose of this study was to find out how thread form designs have an influence on the equivalent stress, equivalent strain, maximum shear stress and maximum shear strain and which design of thread form generates more maximum equivalent stress and strain. 3-D finite element analysis was used to evaluate the stress and strain patterns of three tread types. The results of this study were as follow. 1. Under the 200N of axial load, the value of maximum equivalent stress is smallest in square thread and there is no significant difference between that of V thread and reverse buttress thread. 2. Under the 200N of axial load, the value of maximum equivalent strain is largest in V thread and smallest in square thread. 3. Under the 200N of axial load, the value of maximum shear stress is smallest in square thread and there is no significant difference between that of V thread and reverse buttress thread. 4. Under the 200N of axial load, the value of maximum equivalent strain is largest in V thread and there is no significant difference between that of square thread and reverse buttress thread. 5. Above results show that the square thread has special advantages in stress and strain compared with other thread types, especially in shear stess which is most determinant to implant-bone interface. Considering the superior biomechanical properties of square form implant, we presume that square form implant has better clinical results than the other types of implants in the same clinical conditions.
Telomere Structure, Function, and Maintenance in Plants
김우택,김미경 한국식물학회 2018 Journal of Plant Biology Vol.61 No.3
Telomeres in eukaryotes comprise specific repetitiveDNA sequences and binding proteins. Since their absenceresults in chromosomal end fusions and gene deletions, theyare considered critical for genomic stability. In plants, as inyeasts and mammals, telomeres are essential for normaldevelopment and differentiation. Despite recent discoveriesconcerning plant telomeres, many questions remain aboutthe mechanism of telomere homeostasis in plants. In thisreview, we summarize the roles of telomeres and telomerasebindingproteins in plant biology and explain how the lengthof a plant telomere is regulated.
벼 배유세포에서 (胚乳細胞) 분리된 미크로좀 내로의 저장 (貯藏) 단백질 이동
김우택,Xingxiang Li,Thomas W . Okita (Woo Taek Kim) 한국식물학회 1994 Journal of Plant Biology Vol.37 No.3
Developing rice endosperm cells display two morphologically distinct rough endoplasmic reticulum (ER) membranes, the cisternae ER (C-ER) and the protein body ER (PB-ER), the latter delimiting the prolamine protein bodies. We (Li et al., 1993) have recently shown that the storage protein mRNAs are not randomly distributed on these ER types; the C-ER is enriched for glutelin mRNAs, whereas the PB-ER harbors predominantly prolamine transcripts. To address whether these ER types have different capacities to translate these mRNAs and translocate their proteins into the lumen, a microsomal fraction enriched in C-ER vesicles was prepared from developing rice seeds When present in an in vitro translation system, the microsomes were able to proteolytically remove the signal peptide and internalize both preproglutelin and preprolamine within the microsomal vesicles. Of the two species, preprolamine was more effectively translocated and processed. These results suggest that the C-ER has the capacity to recognize and bind both storage protein mRNAs during protein synthesis. Moreover, efficient translocation and processing of glutelin requires additional factors that are deficient or absent in the in vitro system.
김우택,이경옥,김행미,안두홍 慶北大學校 醫科大學 1986 慶北醫大誌 Vol.27 No.3
A clinical observation was performed on 185 cases of prematurity and low birth weight infants(LBW) who admitted to K.N.U.H. during the past 10 years from Jan. 1976 to Dec. 1985. The incidence of the premature LBW was 76.2%(141 cases) and the sex ratio of male to female was 1.6:1. Concomitant maternal diseases were plasenta previa 21 cases, toxemia 20 cases etc. It was noted that in more than half of these infants the gestational age distributed from 32 to 36 weeks and the birth weight from 1750 to 2250 gm. The mortality rate among the premature and LBW infants was 11.9% and the causes of death were IRDS(45.4%), sepsis(27.2%) and intracranial hemorrhage(18.1%) in the order of frequency. There were neonatal complications such as jaundice(47.0%), hypocalcemia(40.5%), sepsis(30.3%), hypoglycemia(15.7%) and IRDS(10.8%) in the order of frequency. The prematurity and LBW who lost less than 10% of their birth weights were 67.5% and who lost their birth weight more than 15% were 11.9%. 70.8% of prematurity or LBW regain their birth weight within 2week after birth. The smaller the birth weight, the slower the regain of birth weight.