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자연계로부터 Patulin 생성 Penicillium sp.의 분야에 관한 연구
김동술,정덕화,김성영,정선희,강성조,전향숙 한국환경보건학회 1993 한국환경보건학회지 Vol.19 No.3
To study the prevention of mycotoxin contamination, a large number of sample sources were collected, and Penicilliurn sp. were isolated. TLC and HPLC methods were applied to confirm the patulin producing abilities of isolated strains. From 321 sample sources such as fruit (46), rice (31), corn (19), barley (22) and soil (38), 203 strains of Penicilliurn sp. were isolated. By the result of TLC, 21 strains were assumed to be patulin producing strains, but as a result of HPLC, only 17 strains produced patulin. Among them, E-219 strain showed highest patulin level as 0.12 mg/ml broth.
대구·경북지방 곡류중의 Aflatoxin 생성균주의 오염에 관한 연구
김동술,문귀임,오일웅,오현숙,민충식,김은경,이삼룡,안경아,정영숙 식품의약품안전청 1998 식품의약품안전청 연보 Vol.2 No.-
대구·경북지역 농산물 중에 aflatoxin생성균주의 오염실태를 파악하기 위하여 고려, 문경, 상주, 안동, 포항, 성주, 경주, 김천, 구미, 예천, 영천, 영덕 등 12개 지역으로부터 쌀 66점, 보리 44점, 콩 94점, 당콩 36점, 조 37점, 토양 57점 등 총 334점을 수집하여 rose bengal, PDAmSLS, YES등의 배지를 사용하여 Aspergillus flavus및 parasiticus를 분리한 결과 12.6%인 42균주를 분리하엿으며 이들 중 TLC에서 형광을 나타내는 균주는 21%인 9균주였다. 또한 HPLC로 aflatoxin B₁생성능이 있는 균주를 확인하 결과 시룝점(콩)에서 분리되었으며 따라서 aflatoxin 생성능이 있는 aspergillus속 곰팡이으 ㅣ오염률은 0.3%이였다. In order to iBvestigate aflatoxin producing strains from agricultural products in the Taegu and Kyungpook dist·ricts, we collected 334 sarrlples svch as rice (66), barley(44), soybean(94), pea-nut(36), millet(37), soil(57). Using rose bengal, PDA, sLs, YES medium, we isolated the aflatoxin pro-ducing strains, fsfergiHur ffauHs and farasificur. As a result of screening by thin layer chromatography(TLC), 42 straius(21% ) from the 334 samples showed fluorescent spot. Aflatorin Bl was determinedfrom soybean(1) by HPLC- ln consequence, the contarHination percentage of fsfergiffHs sf. which pro-duce aflatoxiu sholved 0-3% .
ELISA법에 의한 mouse의 혈청 및 조직중의 T-2 toxin의 검색
김동술,송재영,정덕화 한국환경보건학회 1996 한국환경보건학회지 Vol.22 No.1
In order to detect the T-2 toxin accumulation in the animal tissues, T-2 toxin, produced by Fusarium sporotrichioides M-1-1, was injected to mouse by 0, 1 and 2 mg per kilogram of body weight, respectively, and T-2 toxin extracted from serum and organs were analyzed by the indirected competitive ELISA. The indirect competitive ELISA established in the laboratory can be check less than 0.1 ppb level of T-2 toxin and average recovery of T-2 toxin spiked was 80~113% in animal samples such as serum, liver and kidney. After 6 weeks of treatment with 2 mg of T-2 toxin per kg body weight, T-2 toxin was accumulated in serum (133.0 ng/ml), liver(1.4 ng/g) and kidney(14.3 ng/g) of mouse injected with 2 mg of toxin per kg body weight
Allylisothiocyanate 가 Aspergillus parasiticus R-716 의 aflatoxin 생성에 미치는 영향
김동술,정덕화 한국식품위생안전성학회 1996 한국식품위생안전성학회지 Vol.11 No.1
The effect of allylisothiocyanate, the major compound of radish on the growth of Aspergillus parasiticus R-716 and aflatoxin production, was investigated. An increase in the level of allylisothiocyanate results in a decrease both growth and aflatoxin per myclial weight, and the addition of 125 ppm allylisothiocynate completely inhibited the growth of this strain. The addition of allylisothiocyanate to the culture of R-716 strain delayed the production of atlatoxin. The inhibition of aflatoxin was more B-group than G-group and M-group during cultural period. The growth of strain and aflatoxin production were greatly affected by the addition of allylisothiocyanate.
Ochratoxin A가 마우스의 장기에 축적 정도와 ELISA법 확립에 관한 연구
김동술 한국식품위생안전성학회 1995 한국식품위생안전성학회지 Vol.10 No.4
Ochratoxin A was produced from Aspergillus ochraceus ATCC 18472 which was then orally administered into the experimental mice to study the toxic levels of ochratoxin A. AELISA (enzyme-linked immunosorbent assay) method which is more rapid and safe than conventional analytical method, was developed by using ochratoxin A antibody. This method was successfully used to measure the levels of ochratoxin Ain blood, liver and kidney of mice. In order to produce a large amount of ochratoxin A to study toxicity in the mice, Aspergillus ochraceus ATCC 18412 was incubated in the rice medium and as a result 0.5 g of ochratoxin A form rice medium (kg) was produced after extraction and purification Feed consumption and gain in body weight of mice with ochratoxin A at a level of $10 \mu\textrm{g}/g$ body weight was significantly (p<0.05) reduced as compared with control during period of 3 weeks. Ochratoxin A-BSA conjugate was made by putting 13 mole of ochratoxin A on I mole of BSA. This conjugate was used to develop ELISA method. The minmum detection level of ochratoxin A by established ELISA method was 0.5 ppb. After oral adminstraton of ochratoxin A dose of $10\;\mu\textrm{g}/g$ every two day for 3 weeks, concentration of ochratoxin A was measured in the blood, liver and kidney by ELISA method. The level of ochratoxin A was $11\;\mu\textrm{g}/dl,\;0.9 \mu\textrm{g}/g\;and\;3.7\;\mu\textrm{g}/g$ in the blood, liver and kidney, respectively.