Development and characterization of flower and capsule in Borneo jewel orchid Macodes limii J.J. Wood and A.L. Lamb (Orchidaceae: Asparagales)

Halyena Indan,Devina David,Roslina Jawan,Hartinie Marbawi,Jualang Azlan Gansau 국립중앙과학관 2021 Journal of Asia-Pacific Biodiversity Vol.14 No.2

Macodes limii J.J. Wood & A.L. Lamb is an endemic jewel orchid of Sabah in Borneo. The species hasunique characteristic with high potential to be commercialized as ornamental plant. However, it wasunderstudied due to its scarcity in nature, which can only be found at certain area in Sabah with ultramaficsoil. To understand the reproduction biology of this species, the present study was conducted todocument the developmental stages of flower and capsule to determine the right harvesting time forfurther use in seed germination study. Macodes limii was collected from its natural habitat and cultivatedex situ throughout this study. Flower development was recorded starting from inflorescence initiationuntil full bloom, which took over 54 days to complete. Flower was artificially pollinated, and capsule wasproduced within 22 days, and harvesting was performed prior to dehiscence. Seeds were asepticallycultured on half-strength Murashige and Skoog medium þ 3% (w/v) sucrose, and 29% of seeds weresuccessfully germinated after 24 weeks under continuous dark condition. The current findings arebeneficial as a reference to support the conservation efforts of this endemic species.

The Study on Flora and Distribution Characteristics of Orchidaceae on Moor

Rho, Jae-Hyun 한국환경과학회 2003 한국환경과학회지 Vol.12 No.2

Total 7 moors. Yong-neup on Mt. Daeam, Chilbo Mt. moor, Dogapsa-neup in Mt. Wolchul. Wangdeungjae-neup on Mt. Jiri, Mujechi-neup on Mt. Jungjok, Buljae-neup on Mt. Kyungak and Geoinri-neup on Dongsang, were investigated by literatures and survey. Distribution, condition, and similarity of dominant species and Orchidaceae on moor were analyzed. The results are follows; There are total 46 wetland plant species observed more than twice from 7 moors. The plants classified as constancy class Ⅴ, which are appeared more than 80%, are Iris ensata var. spontane, Juncus effusus var. decipiens, Sanguisorba tenuifolia var. alba, Habenaria linearifolia, Sium ninsi, Lobella sessilifolia, Lastrea thelyptoris, and Eupatorium chinensis var. simplicifolium. Highly apperaing Orchidaceae, which was classified to constancy class Ⅳ plant, are Habenaria livearifolia, Pogonia japonica, Epipactis thunbergii, and Platanthera mandarinorum. They are the typical index plants of the moors. Habitual enviroment of Habenaria rodiata and Platanthera hologlottis can be affected by environmental aspects such as latitude, altitude and the water quality. However those of Habenaria linearifolia, Epipactis thunbergii, and Pogonia japonica are not affected by latitude and altitude, but may be determined by water condition of wetland, As the result of similarity index analysis for appearance of Orchidaceae identifying each moors in the view off species composition, swamps at Dogapas-neup and Buljae-neup. Yong-neup had shown the high similarity indices of 0.85 and 0.75 with Wangdeungjae-neup and Mujechi-neup, respectively. Growth properties of wild Orchidaceae at wetland were almost similar. Platanthera mandarinorum and Liparis kumokiri had characteristics off good adaptability. Most of them need light. Pogonia was the only long-day plant. The flowering time of these Orchidaceae was from May to September.

우리나라 미기록 식물: 영아리난초(난초과)

김찬수,문명옥,고정군 한국식물분류학회 2009 식물 분류학회지 Vol.39 No.3

Nervilia nipponica Makino (Orchidaceae), a genus and species previously unrecorded for the Korean flora, was collected in the deciduous forests of Mt. Halla, Jeju Island. This species was known to be distributed mainly from tropical to subtropical regions of Taiwan and Japan. Genus Nervilia is distinguished from other genera of tribe Nervilieae (Orchidaceae) by having only a single, plicate leaf which develops and senesces before flowering shoots appear, stems unbranched, two pollinia, and sometimes lacking a viscidium. 제주도 한라산 낙엽수림에서 우리나라 미기록속인 난초과 영아리난초속의 영아리난초가 채집되었다. 본 종은 대만과 일본의 열대 및 아열대에 주로 분포하는 것으로 알려져 왔다. 영아리난초속은 식물체가 흔히 녹색이며 잎은 단생이고 주름이 있으며, 화아가 성장하기 전에 발달하고 낙엽이 되며, 줄기는 분지하지 않고, 화분괴는 2개이며, 대부분 점착체가 없는 점에서 난초과 Nervilieae족의 다른 속과 뚜렷하게 구분된다.

다도새우난초(난초과): 새우난초속의 한반도 미기록종

홍행화,김종선,장길훈,임형탁 한국식물분류학회 2010 식물 분류학회지 Vol.40 No.3

We report a unrecorded species, Da-do-sae-u-nan-cho (Calanthe x kibanakirishima F. Maek). It was discovered on Heuksan Island in Jeollanam Province. The species differs from C. aristulifera in the corolla color and the short, wide spur. It is also easily distinguished from the other species of Calanthe by the simple trichomes on the abaxial surface of its leaves and spur. 전라남도 신안군 흑산면에서 한반도 미기록종인 다도새우난초(Calanthe × kibanakirishima F. Maek.)를 발견하여 보고한다. 다도새우난초는 신안새우난초와 비슷하지만 화색이 다르고, 거가 짧고 더 굵다는 점에서 다르다. 국내의 다른 새우난초속 종들과는 잎의 뒷면에 털이 있고 거가 발달한다는 점에서 쉽게 구별된다.

Floral Volatile Compound Accumulation and Gene Expression Analysis of Maxillaria tenuifolia

Su Yeong Kim,Mummadireddy Ramya,Hye Ryun An,Pil Man Park,Su Young Lee,So-Young Park,Pue Hee Park 한국원예학회 2019 원예과학기술지 Vol.37 No.6

Floral volatiles play a key role in attracting pollinators, and floral scent is an important trait for many floricultural crops, including orchids. Maxillaria tenuifolia belongs to the Orchidaceae family and has highly fragrant flowers. Due to the importance of plant biology, we needed to isolate the floral volatiles and corresponding genes in this plant. In this study, we investigated the floral volatile compounds and gene expression of M. tenuifolia at various flower developmental stages and in different floral organs using headspace–solid-phase microextraction–gas chromatography–mass spectrometry (HS-SPME-GC-MS) and quantitative real time PCR (qRT-PCR). The main compounds of M. tenuifolia were sesquiterpene, known as the scent of coconut, and the compounds β-caryophyllene, α-copaene, and δ-decalacton. The total amounts of volatile compounds in petal, sepal, lip, and column was 94.0%, 93.0%, 72.7%, and 90.0%, respectively. The compounds α-copaene, β- caryophyllene, and caryophylladienol II were identified in all parts. The total volatile compound amount at the bud stage (I) was 29.1%, at the initial flowering stage (II) was 81.0%, at the full flowering stage (III) was 93.7%, at the loss of pedicel color stage (IV) was 85.6%, and at the wilting flower stage (V) was 69.8%. Except for the bud stage (I), α-copaene and β-caryophyllene were identified in all stages. In the qRT-PCR analysis, most of the terpenoid genes were highly expressed at the full flowering stage in the sepal and petal. According to these results, we concluded that sesquiterpene is the major source for the floral scent profile in this plant. This study establishes a baseline for product development and provides information about using aromatics to promote orchid consumerism by identifying and analyzing volatile compounds and gene expression.

First record of Androcorys pusillus (Orchidaceae) from Mt. Baekdu

이정심,김수남,김무열 한국식물분류학회 2013 식물 분류학회지 Vol.43 No.1

Androcorys pusillus (Ohwi & Fukuy.) Masam. (Orchidaceae) was first found from Mt. Baekdu of the Korean peninsular and was known to distribute from Taiwan and Japan. This species is distinguished from the related taxa by its denticulated sepal margin, petal width which is as broad as dorsal sepal, and larger bract size.

A New Citryl Glycoside from <i>Gastrodia elata</i> and Its Inhibitory Activity on GABA Transaminase

Choi, Jung-Hyun,Lee, Dong-Ung The Pharmaceutical Society of Japan 2006 Chemical & pharmaceutical bulletin Vol.54 No.12

<P>A new citryl glycoside, trimethylcitryl-β-<SMALL>D</SMALL>-galactopyranoside (1) along with a known phenolic compound, gastrodigenin (2) have been isolated from the active fraction of the rhizomes of <I>Gastrodia elata</I> (Orchidaceae). Their structures were elucidated on the basis of spectroscopic data and chemical reaction. 1 inhibited GABA transaminase activity by 56.8% at the final concentration of 10 μg/ml.</P>

Orchid floral volatiles: Biosynthesis genes and transcriptional regulations

Ramya, Mummadireddy,An, Hye Ryun,Baek, Yun Su,Reddy, Kondreddy Eswar,Park, Pue Hee Elsevier 2018 Scientia horticulturae Vol.235 No.-

<P>Floral scent and colour are key trait for many floricultural crops. Floral volatiles are biologically and economically important plant derived compounds and they play vital role in pollinator attraction, plant defense and interaction with surrounding the environment. Orchidaceae is one of the largest and most widespread families of flowering plants, with more than 25,000 species. Orchids are one of the most significant plants in ecologically adopted every habitation on earth. Floral volatiles is not limited in its role to pollinator attraction, it's widely used for perfumes, cosmetics, flavourings and therapeutic applications. Even though biochemistry of floral volatiles is still new field for investigation. Due to importance of the plant biology orchid floral scent research were deeply needed. Analysis of the biosynthesis mechanisms involved in floral scent is necessary to understand the fine-scale molecular functions and to breed to new cultivars through floral volatile genetic engineering approaches. Now a days many researchers developed floral scent models in plants. However, little is known about the pathways responsible for floral scent in orchids, due to the long life cycle and large genome size. Only some terpenoid pathways were reported in orchids. In this review, we mainly concentrated orchid floral volatile regulation and compounds synthesis responsible pathways. Moreover,we mentioned emission of orchid floral volatiles and their function in pollination ecology. This information will provide a basic information on orchid floral scent research.</P>

First record of Neottia japonica (Orchidaceae) from Korea

소지현,정미숙,정영순,이남숙 한국식물분류학회 2013 식물 분류학회지 Vol.43 No.3

Neottia japonica (Blume) Szlach. (Orchidaceae), previously known to be native in southern part of Japan and Taiwan, was found in Seogwipo-si, Jeju-do, Korea. This species is distinguished from the related taxa of Neottia pinetorum (Lindle.) Szlach and N. nipponica (Makino) Szlach. by smaller plant, lip with T-shaped lamella and auricles embracing column, and flowering in the spring. The local name was given as “A-gi-ssangnip-nan-cho” based on the cuter and smaller plant than N. pinetorum. The description and illustrations of the species on morphological characters, voucher specimens, and photograph at the habitat are provided.

난과식물의 생약학적 연구

도정애,Toh, Chung-Ae 한국생약학회 1994 생약학회지 Vol.25 No.3

In order to estimate accurate originality of the important crude drugs, Gastrodia, Dendrobium, Bletilla and Cremastra spp. were investigated comparing their morphological, anatomical and physicochemical characteristics and antibacterial, antifungal activities. The results of the studies as follows: 1. In morphological and anatomical studies, Korean Gastrodia contained more mucilage duct and symbiotic fungi than imported Gastrodia. Korean Dendrobium contained starch grains but without starch grains in the imported Dendrobium from China enclosed with thicken silicified wall. The corm of Cremasta appendiculata contained raphides of bundles with mucilage while the bulbs of Tulipa edulis contained several various starch grains form. 2. In physiological and TLC analysis, crude drugs in Orchidaceae contained common constituents with fluorescence and much mucilage. 3. The EtOH extracts of Gastrodia, Dendrobium, Bletilla, Cremastra showed antibacterial activities against B. subtilis and E. coli (Dendrobium>Gastrodia>Bletilla>Cremastra). But, no antifungal activities against C. albicans, A. niger were observed.