Orchid floral volatiles: Biosynthesis genes and transcriptional regulations

Ramya, Mummadireddy,An, Hye Ryun,Baek, Yun Su,Reddy, Kondreddy Eswar,Park, Pue Hee Elsevier 2018 Scientia horticulturae Vol.235 No.-

<P>Floral scent and colour are key trait for many floricultural crops. Floral volatiles are biologically and economically important plant derived compounds and they play vital role in pollinator attraction, plant defense and interaction with surrounding the environment. Orchidaceae is one of the largest and most widespread families of flowering plants, with more than 25,000 species. Orchids are one of the most significant plants in ecologically adopted every habitation on earth. Floral volatiles is not limited in its role to pollinator attraction, it's widely used for perfumes, cosmetics, flavourings and therapeutic applications. Even though biochemistry of floral volatiles is still new field for investigation. Due to importance of the plant biology orchid floral scent research were deeply needed. Analysis of the biosynthesis mechanisms involved in floral scent is necessary to understand the fine-scale molecular functions and to breed to new cultivars through floral volatile genetic engineering approaches. Now a days many researchers developed floral scent models in plants. However, little is known about the pathways responsible for floral scent in orchids, due to the long life cycle and large genome size. Only some terpenoid pathways were reported in orchids. In this review, we mainly concentrated orchid floral volatile regulation and compounds synthesis responsible pathways. Moreover,we mentioned emission of orchid floral volatiles and their function in pollination ecology. This information will provide a basic information on orchid floral scent research.</P>

Isolation and Expression Analysis of Cold Responsive Genes in Cymbidium goeringii

Ramya Mummadireddy,Su Young Lee,Hye Ryun An,Pil Man Park,Pue Hee Park 한국원예학회 2019 한국원예학회 학술발표요지 Vol.2019 No.10

Floral Volatile Compound Accumulation and Gene Expression Analysis of Maxillaria tenuifolia

Su Yeong Kim,Mummadireddy Ramya,Hye Ryun An,Pil Man Park,Su Young Lee,So-Young Park,Pue Hee Park 한국원예학회 2019 원예과학기술지 Vol.37 No.6

Floral volatiles play a key role in attracting pollinators, and floral scent is an important trait for many floricultural crops, including orchids. Maxillaria tenuifolia belongs to the Orchidaceae family and has highly fragrant flowers. Due to the importance of plant biology, we needed to isolate the floral volatiles and corresponding genes in this plant. In this study, we investigated the floral volatile compounds and gene expression of M. tenuifolia at various flower developmental stages and in different floral organs using headspace–solid-phase microextraction–gas chromatography–mass spectrometry (HS-SPME-GC-MS) and quantitative real time PCR (qRT-PCR). The main compounds of M. tenuifolia were sesquiterpene, known as the scent of coconut, and the compounds β-caryophyllene, α-copaene, and δ-decalacton. The total amounts of volatile compounds in petal, sepal, lip, and column was 94.0%, 93.0%, 72.7%, and 90.0%, respectively. The compounds α-copaene, β- caryophyllene, and caryophylladienol II were identified in all parts. The total volatile compound amount at the bud stage (I) was 29.1%, at the initial flowering stage (II) was 81.0%, at the full flowering stage (III) was 93.7%, at the loss of pedicel color stage (IV) was 85.6%, and at the wilting flower stage (V) was 69.8%. Except for the bud stage (I), α-copaene and β-caryophyllene were identified in all stages. In the qRT-PCR analysis, most of the terpenoid genes were highly expressed at the full flowering stage in the sepal and petal. According to these results, we concluded that sesquiterpene is the major source for the floral scent profile in this plant. This study establishes a baseline for product development and provides information about using aromatics to promote orchid consumerism by identifying and analyzing volatile compounds and gene expression.

Floral Volatile Compound Accumulation and Gene Expression Analysis of Maxillaria tenuifolia

Su Yeong Kim,Mummadireddy Ramya,Hye Ryun An,Pil Man Park,Su Young Lee,So-Young Park,Pue Hee Park(박부희) 한국원예학회 2020 한국원예학회 학술발표요지 Vol.2020 No.11

Effects of Preservatives on Vase Life of Cut Cymbidium Flowers Bred in Korea

Kim, Su Yeoung,An, Hye Ryun,Baek, Yun Su,Ramya, Mummadireddy,Park, Pue Hee Korean Society for Floricultural Science 2017 화훼연구 Vol.25 No.2

Homology differences between complete Sacbrood virus genomes from infected Apis mellifera and Apis cerana honeybees in Korea

Reddy, Kondreddy Eswar,Yoo, Mi Sun,Kim, Young-Ha,Kim, Nam-Hee,Ramya, Mummadireddy,Jung, Ha-Na,Thao, Le Thi Bich,Lee, Hee-Soo,Kang, Seung-Won Springer-Verlag 2016 Virus genes Vol.52 No.2

<P>Sacbrood virus (SBV) represents a serious threat to the health of managed honeybees. We determined four complete SBV genomic sequences (AmSBV-Kor1, AmSBV-Kor2, AcSBV-Kor3, and AcSBV-Kor4) isolated from Apis mellifera and Apis cerana in various regions of South Korea. A phylogenetic tree was constructed from the complete genomic sequences of these Korean SBVs (KSBVs) and 21 previously reported SBV sequences from other countries. Three KSBVs (not AmSBV-Kor1) clustered with previously reported Korean genomes, but separately from SBV genomes from other countries. The KSBVs shared 90-98 % identity, and 89-97 % identity with the genomes from other countries. AmSBV-Kor1 was least similar (similar to 90 % identity) to the other KSBVs, and was most similar to previously reported strains AmSBV-Kor21 (97 %) and AmSBV-UK (93 %). Phylogenetic analysis of the partial VP1 region sequences indicated that SBVs clustered by host species and country of origin. The KSBVs were aligned with nine previously reported complete SBV genomes and compared. The KSBVs were most different from the other genomes at the end of the 5' untranslated region and in the entire open reading frame. A SimPlot graph of the VP1 region confirmed its high variability, especially between the SBVs infecting A. mellifera and A. cerana. In this genomic region, SBVs from A. mellifera species contain an extra continuous 51-nucleotide sequence relative to the SBVs from A. cerana. This genomic diversity may reflect the adaptation of SBV to specific hosts, viral cross-infections, and the spatial distances separating the KSBVs from other SBVs.</P>

Recent Progress on Genetic Modification in Floriculture

Chang Ho Ahn,Pue Hee Park,Pil Man Park,Hye Ryun An,Mummadireddy Ramya,Su Young Lee 한국화훼학회 2019 화훼연구 Vol.27 No.4

화훼작물의 새로운 품종 개발과 특성을 개선하기 위해 교잡 및 돌연변이 육종과 같은 기존 기술에서 유전자변형기술에 이르기까지 다양한 육종 기술이 사용되고 있으며, 특히 화훼류의 유전자변형기술은 화색변형, 화형 및 형태변형, 개화시기 조절, 꽃 수명연장, 내환경성, 내병성, 내충성 등의 목적으로 이루어지고 있다. 국내에서의 GM 화훼류 연구는 2000년대부터 진행되었고, 현재는 장미, 국화, 카네이션, 페튜니아 등을 대상으로 형질전환체를 개발하고 있다. 호주의 Florigene사에서 개발한 푸른 카네이션이 상업화되면서 일본 SUNTORY사 의 파란색 장미 그리고 호주 Florigene사의 시들지 않는 카네이션이 개발되어 상용화되고 있으며, 더 많은 품종 개발과 판매 시장이 증가하고 있는 추세이다. 이처럼 GM 화훼작물에 대한 연구성과는 급속도로 발전하고 있으며, 국내에서도 생명 공학 기술을 기반으로 형질이 우수하고 소비자의 선호도가 높은 새로운 화훼 신품종 개발로 수출확대를 도모함으로써 농가 소득 향상을 기대할 수 있을 것으로 판단된다. 본 보고에서는 국내외에 최근 보고된 GM 화훼작물의 형질전환체 연구개발 성공 사례들을 소개하고, 그 요인 분석을 통해 향후 더 성공적인 GM 화훼작물 개발 전략을 수립하는데 도움이 되고자 한다. Plant transformation technology (or genetic modification), along with breeding techniques such as cross-breeding and mutation breeding, has been widely used for breeding new varieties and enhancing the characteristics of floricultural plants. In particular, genetically modified (GM) floriculture has been developed for modified characteristics such as floral color, pigmentation, stress tolerance, disease resistance, and fragrance. In South Korea, studies on GM floriculture have been under progress since the 2000s, and there have been various research projects involving GM floriculture. In the global flower market, a mauve-colored carnation called “MoondustTM” was first developed and commercialized by the Australian biotechnology company Florigene, and a blue rose called “APPLAUSETM” was developed and commercialized by the Japanese company SUNTORY. Furthermore, new varieties of GM floriculture have recently been developed, and international trade in ornamental plant materials has grown. Given the rapid increase of studies on GM floriculture and the growing global market for GM ornamental plants, biotechnology may be used to increase farm household income by developing new ornamental varieties that have superior characteristics and match consumer preferences. In t his review, we summarize t he r esearch on G M ornamental plants during the last four years. This information will help in establishing more successful strategies for developing GM floriculture. Moreover, this report will facilitate the breeding of new and improved varieties with modified growth rates and superior product quality, including changes in flower color, fragrance, and stress tolerance.

Molecular characterization and phylogenetic analysis of deformed wing viruses isolated from South Korea

Reddy, Kondreddy Eswar,Noh, Jin Hyeong,Yoo, Mi-Sun,Kim, Young-Ha,Kim, Nam-Hee,Doan, Huong Thi Thanh,Ramya, Mummadireddy,Jung, Suk-Chan,Van Quyen, Dong,Kang, Seung-Won Elsevier 2013 Veterinary microbiology Vol.167 No.3

<P><B>Abstract</B></P> <P>Deformed wing virus (DWV) is one of the most common viral infection in honeybees. Phylogenetic trees were constructed for 16 partial nucleotide sequences of the structural polyprotein region and the RNA helicase region of South Korean DWVs. The sequences were compared with 10 previously reported DWV sequences from different countries and the sequences of two closely related viruses, Kakugo virus (KGV) and <I>Varroa destructor</I> virus-1 (VDV-1). The phylogeny based on these two regions, the Korean DWV genomes were highly conserved with 95–100% identity, while they also shared 93–97% similarity with genotypes from other countries, although they formed a separate cluster. To investigate this phenomenon in more detail, the complete DWV genome sequences of Korea-1 and Korea-2 were determined and aligned with six previously reported complete DWV genome sequences from different countries, as well as KGV and VDV-1, and a phylogenetic tree was constructed. The two Korean DWVs shared 96.4% similarity. Interestingly, the Korea-2 genome was more similar to the USA (96.5%) genome than the Korea-1. The Korean genotypes highly conserved with USA (96%) but low similarity with the United Kingdom3 (UK3) genome (89%). The end of the 5′ untranslated region (UTR), the start of the open reading frame (ORF) region, and the 3′ UTR were variable and contained several substitutions/transitions. This phenomenon may be explained by intramolecular recombination between the Korean and other DWV genotypes.</P>

장미 품종(‘글로리웨딩’, ‘미샤’, ‘핑크뷰티’)의 체세포배발생 캘러스 안정적인 유도 조건 구명

안창호(Chang Ho Ahn),김란선(Nan Sun Kim),신주영(Ju Young Shin),박부희(Pue Hee Park),박필만(Pil Man Park),안혜련(Hye Ryun An),뮤라마디레디 람야(Ramya Mummadireddy),이수영(Su Young Lee) 한국원예학회 2019 한국원예학회 학술발표요지 Vol.2019 No.10