상표소와 상심자가 난소적출로 유발된 흰쥐의 골다공증 치료효과에 미치는 영향

이재우,서부일,박지하,노성수,김용현,김미려,Lee, Jae-Woo,Seo, Bu-Il,Park, Ji-Ha,Roh, Seong-Soo,Kim, Yong-Hyun,Kim, Mi-Ryeo 대한본초학회 2009 大韓本草學會誌 Vol.24 No.1

Objectives:The present study has been undertaken to investigate the effects of Mantidis $O\ddot{O}theca$ and Mori Fructus on treatment of osteoporosis in ovariectomized rats. Methods: In this experiment, the rats were ovariectomized. Rats were administered by Mantidis $O\ddot{O}theca$ and Mori Fructus. The levels of bone mineral density, osteocalcin.ALP.calcium.phosphorus in serum, calcium. phosphorus.deoxypyridinoline in urine and calcium.phosphorus.ash weight in bone were measured. Results: 1. The levels of femoral and fibula-tibial bone mineral density were significantly increased in comparison with OVX group at 4, 8 weeks in Mantidis $O\ddot{O}theca$ group. And the levels of femoral and fibula-tibial bone mineral density were significantly increased in comparison with OVX group at 8 weeks in Mori Fructus group. 2. The levels of serum osteoclacin and ALP showed significant decrease in comparison with OVX group at 4, 8 weeks in Mantidis $O\ddot{O}theca$ and Mori Fructus group. The levels of serum calcium showed significant decrease in comparison with OVX group at 4 weeks in Mantidis $O\ddot{O}theca$ and Mori Fructus group. The levels of serum phosphorus showed significant decrease in comparison with OVX group at 4, 8 weeks in Mantidis $O\ddot{O}theca$ and Mori Fructus group. 3. The levels of urine calcium, phosphorus and deoxypyridinoline showed significant decrease in comparison with OVX group in Mantidis $O\ddot{O}theca$ and Mori Fructus group. 4. The levels of fibula-tibial calcium and phosphorus showed significant increase in comparison with OVX group in Mantidis $O\ddot{O}theca$ group and Mori Fructus group. The levels of femoral calcium and phosphorus showed significant increase in comparison with OVX group in Mori Fructus group. The levels of femoral and fibula-tibial ash weight showed significant increase in comparison with OVX group in Mantidis $O\ddot{O}theca$ group and Mori Fructus group. Conclusions: Reviewing these experimetal results, it appeared that Mantidis $O\ddot{O}theca$ and Mori Fructus had efficacy on treatment of osteoporosis.

Mori Fructus Induces Cell Death through ROS-dependent Mitochondrial Apoptotic Pathway in Human Glioma Cells

장상원,정지천 한의병리학회 2008 동의생리병리학회지 Vol.22 No.5

Mulberry has been reported to contain wide range of polyphenols and have chemopreventive activity. However, little has been known regarding the effect of mulberry fruits on cell viability in human glioma cells. The present study was undertaken to examine the effect of mulberry fruit (Mori Fructus) on cell viability and to determine its underlying mechanism in human glioma cells. Cell viability and cell death were estimated by MTT assay and trypanblue exclusion assay, respectively. Reactive oxygen species (ROS) generation was measured using the fluorescence probe DCFH-DA. The mitochondrial transmembrane potential was measured with DiOC6(3). Bax expression and cytochrome c release were measured by Western blot analysis. Caspase activity was estimated using colorimetric kit. Mori Fructus resulted in apoptotic cell death in a dose- and time-dependent manner. Mori Fructus increased ROS generation and the Mori Fructus-induced cell death was also prevented by antioxidants, suggesting that ROS generation plays a critical role in Mori Fructus-induced cell death. Western blot analysis showed that Mori Fructus treatment caused an increase in Bax expression, which was inhibited by the antioxidant N-acetylcysteine (NAC). Mori Fructus induced depolarization of mitochondrial membrane potential and its effect was inhibited by the antioxidants NAC and catalase. Mori Fructus induced cytochrome c release, which was inhibited by NAC. Caspase activity was stimulated by Mori Fructus and caspase inhibitors prevented the Mori Fructus-induced cell death. These findings suggest that Mori Fructus results in human glioma cell death through ROS-dependent mitochondrial pathway in human glioma cells.

상표소(桑표소)와 상침자(桑침子)가 난소적출로 유발된 흰쥐의 골다공증 치료효과에 미치는 영향

이재우 ( Jae Woo Lee ),서부일 ( Bu Il Seo ),박지하 ( Ji Ha Park ),노성수 ( Seong Soo Roh ),김용현 ( Yong Hyun Kim ),김미려 ( Mi Ryeo Kim ) 대한본초학회 2009 大韓本草學會誌 Vol.24 No.1

Objectives: The present study has been undertaken to investigate the effects of Mantidis Ootheca and Mori Fructus on treatment of osteoporosis in ovariectomized rats. Methods: In this experiment, the rats were ovariectomized. Rats were administered by Mantidis Ootheca and Mori Fructus. The levels of bone mineral density, osteocalcin·ALP·calcium·phosphorus in serum, calcium·phosphorus·deoxypyridinoline in urine and calcium·phosphorus·ash weight in bone were measured. Results: 1. The levels of femoral and fibula-tibial bone mineral density were significantly increased in comparison with OVX group at 4, 8 weeks in Mantidis Ootheca group. And the levels of femoral and fibula-tibial bone mineral density were significantly increased in comparison with OVX group at 8 weeks in Mori Fructus group. 2. The levels of serum osteoclacin and ALP showed significant decrease in comparison with OVX group at 4, 8 weeks in Mantidis Ootheca and Mori Fructus group. The levels of serum calcium showed significant decrease in comparison with OVX group at 4 weeks in Mantidis Ootheca and Mori Fructus group. The levels of serum phosphorus showed significant decrease in comparison with OVX group at 4, 8 weeks in Mantidis Ootheca and Mori Fructus group. 3. The levels of urine calcium, phosphorus and deoxypyridinoline showed significant decrease in comparison with OVX group in Mantidis Ootheca and Mori Fructus group. 4. The levels of fibula-tibial calcium and phosphorus showed significant increase in comparison with OVX group in Mantidis Ootheca group and Mori Fructus group. The levels of femoral calcium and phosphorus showed significant increase in comparison with OVX group in Mori Fructus group. The levels of femoral and fibula-tibial ash weight showed significant increase in comparison with OVX group in Mantidis Oo theca group and Mori Fructus group. Conclusions: Reviewing these experimetal results, it appeared that Mantidis Ootheca and Mori Fructus had efficacy on treatment of osteoporosis.

상침자의 항산화 효과 및 3T3-L1 세포주의 유전자 발현에 미치는 영향

박병철 ( Byung Cheol Park ),차윤엽 ( Yun Yeop Cha ) 한방재활의학과학회 2007 한방재활의학과학회지 Vol.17 No.3

Objectives:In recent years, most research has focused on anti-aging and the extension of human life.The prevalence of obesity at all ages is becoming an epidemic around the world.Recent research has shown a positive effect on anti-aging, obesity.Methods:To identify the antioxidant effect, there were four independent experiments were carried out.They were DPPH radical scavenging activity, Nitric oxide(NO) radical activity, Superoxide anion radical scavenging activity and using Bovine serum albumin(BSA).One independent experiment was conducted to identify the candidate gene responsible for the gene expression profile of 3T3-L1 preadipocytes by Oligonucleotide chip microarray.Results and Conclusions:1.The results showed that the ability of DPPH radical`s elimination was increased by the rising concentration of Mori Fructus.When the concentration of Mori Fructus was 2.5 mg/ml, the ability of the DPPH radical`s elimination was at a maximum.2.The ability of the NO radical`s elimination was maximum when the concentration of Mori Fructus was 5 mg/ml.3.The ability of Superoxide anion radical`s elimination was maximum when the concentration of Mori Fructus was 0.3125 mg/ml.4.Antioxidative effects with BSA increased by the rising concentration of Mori Fructus.5.Eleven kinds of gene(C3, Npy1r, Sort1, Agrp, Mod1, Cebpa, Hmox1, Hspb2, G6pd2, Tgfb1, Cebpb) expression increased and two(Ghr, Pik3r1) decreased when Mori Fructus extract was added.So I guess that Mori Fructus has effects of antioxidation, anti-obesity, etc.

상심자의 급성독성에 관한 연구

장보윤,김선범,이미경,김성연 한국생약학회 2012 생약학회지 Vol.43 No.2

Acute toxicity on the water extract of Mori Fructus was examined in male and female mice. The water extract of Mori Fructus was orally administered at a dose of 5 mg/kg, 50 mg/kg, 300 mg/kg and 2,000 mg/kg and had been observed for two weeks. No mortality and abnormal clinical signs were shown for the observation period. At the terminal sacrifice, there were no difference in net body weight gain, organ weight and gross pathological findings among the groups treated with different doses of the water extract of Mori Fructus. The results suggested that under the condition employed in this study LD_50would be more than 2,000 mg/kg. All the data obtained the experiments lead to the water extract of Mori Fructus should have very low acute toxicity.

상심자와 건조상심자 추출물의 항산화 효과 및 전지방세포 분화억제 효과에 관한 연구

김형구 ( Hyung Gu Kim ),왕경화 ( Jing Hua Wang ),임동우 ( Dong Woo Lim ),채희성 ( Hee Sung Chae ),진영원 ( Young Won Chin ),최한석 ( Han Seok Choi ),김호준 ( Ho Jun Kim ) 한방재활의학과학회 2014 한방재활의학과학회지 Vol.24 No.4

ObjectivesThis study was to investigate the antioxidative capacity, antiobesity effect and anti-diabetes effects of Mori Fructus and dried Mori Fructus in Raw 264.7 cells and 3T3-L1 cells.Methods3 different types of Mori Fructus extracts (water 100%, ethanol 30%, ethanol 100%) were used in this study. And 3 different types of dried Mori Fructus extracts (water 100%, ethanol 30%, ethanol 100%) were used in this study. Total polyphenol compund, total favonoid compound, DPPH radical scavenging, ROS activity, NO, cell proliferation were measured in the experiment. Expressions of adipogenic transcription factors including C/EBP-α, C/EBP-β, PPAR-α, PPAR-γ, AMPK-α were analyzed by Real time PCR. ResultsMori Fructus extracts measurements are higher than dried Mori Fructus extracts measurements at Total flavonoid compound and total flavonoid compound. Mori Fructusextracts measurements are lower than dried Mori Fructus extracts measurements at DPPH radical scavenging, ROS activity, NO. In RT-PCR analysis, there is a tendency that dried Mori Fructus extracts inhibit the expression of C/EBP-α, C/EBP-β genes. In RT-PCR analysis, there is a tendency that dried Mori Fructus extracts promote the expression of PPAR-α, PPAR-γ, AMPK-α genes. ConclusionsMori Fructus is effective on inhibiting the oxidation and dried Mori Fructus is effective on inhibiting the obesity and diabetes. (J Korean Med Rehab 2014;24(4):1-13)

Streptozotocin 유발 당뇨 백서에 상엽 지각(桑葉 枳殼) 혼합물의 항당뇨 및 혈관탄성개선 효과

박정섭,박종형,전찬용,최유경,황귀서,김동우,Park, Jung-Sup,Park, Chong-Hyeong,Jun, Chan-Yong,Choi, You-Kyung,Hwang, Gwi-Seo,Kim, Dong-Woo 대한한방내과학회 2007 大韓韓方內科學會誌 Vol.28 No.3

Objectives : This study was designed to evaluate the anti-diabetes and vasoelasticity effects of Mori Folium and Aurantii Fructus in streptozotocin-induced type II diabetes mellitus model. Methods : The anti-diabetic effect of Mori Folium and Aurantii Fructus on rats induced with diabetes by streptozotocin was investigated through analyses of changes in body weight, blood glucose, urine volume of rats, viability of human umbilical vein endothelial cells(HUVECs), and elasticity of descending thoracic aorta in rats. The subjects in this study were divided into four groups(n=15): a normal group without any treatment (Con), a normal group with Mori Folium and Aurantii Fructus treatment(Con+P), a diabetes group induced by streptozotocin(STZ), and a Mori Folium and Aurantii Fructus treatment group under diabetes induced by streptozotocin(STZ+P). Rats were administered streptozotocin to induce diabetes. Results : The study showed that Mori Folium and Aurantii Fructus significantly reduced highly increased blood glucose levels(p<0.01) and prevented the diabetic rats from weight loss(p<0.01) and polyurea(p<0.05), Mori Folium and Aurantii Fructus also recovered decreased viability of HUVECs(p<0.01) and damaged elasticity of aorta induced by the streptozotocin (p<0.01). Conclusions: It was concluded from the results that Mori Folium and Aurantii Fructus have a distinct anti-diabetes effect and they also prevent damage of blood vessel induced by diabetes. resulting in prevention of cardiovascular diseases ascribed to diabetes.

상침자(桑침子)가 3T3-L1 전지방세포 분화시 cytokine에 미치는 영향

박병철 ( Byung Cheol Park ),차윤엽 ( Yun Yeop Cha ) 한방비만학회 2007 한방비만학회지 Vol.7 No.2

In recent years, we are concerned about anti-aging and longevity. And I heard that Mori Fructus has been used for promotion of health in local clinics. So I wanted to know immuno-ability of Mori Fructus and carried out this experiment. Methods We cultivated 3T3-L1 Preadipocytes and Protein chip used for ProteoPlexTM 16-Well Murine Cytokine Array Kit. Results We known the immunity of Mori Fructus about 3T3-L1 Preadipocytes and gained the increase of Cytokines(IL-2, IL-4, IL-12p70, GM-CSF, INF-γ, TNF-α). Conclusions So I guess that Mori Fructus has effect of immuno-ability, etc.

UVB에 노출된 HaCaT 인간 각질세포에서 상심자의 보호효과

이종록,박숙자 대한본초학회 2023 大韓本草學會誌 Vol.38 No.5

Objective : Mori Fructus has been used to relieve thirst, and treat dizziness, tinnitus, and insomnia caused by poor constitution. This study was performed to investigate the protective effect of the ethanol extract of Mori Fructus (MF) in ultraviolet B (UVB)-induced apoptotic cell death in human keratinocyte cells. Methods : MF was prepared by extracting 100 g of Mori Fructus in 1 L of 100% ethanol for 48 h. 3-(4,5- dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was used to measure cell viability. Apoptosis was determined by terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay, and the expression of apoptosis-related proteins was observed by Western blot. Tyrosinase activity was measured with a colorimetric commercial kit. Results : MF promoted cell vitality and inhibited apoptosis of UVB-induced HaCaT cells. MF pretreatment reduced TUNEL-positive cells and increased the expression of caspase-3 and –9. MF also displayed antioxidant effect with high radical scavenging ability. At 2 ㎎/㎖ concentration, the 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical inhibition rates were 55.3 ± 4.6% and 48.5 ± 1.3%, respectively. Furthermore, MF showed a concentration-dependent inhibitory effect on tyrosinase. Conclusion : These results suggest that MF functions as a protective regulator in UVB-induced HaCaT cells by regulating apoptosis and partially exerting antioxidant effects. In addition, the tyrosinase inhibitory effect of MF shows the potential for MF to be used for skin pigmentation.

Toll-like receptor 4-mediated immunoregulation by the aqueous extract of Mori Fructus

Yang, Xin-Ying,Park, Gil-Soon,Lee, Moo Hyung,Chang, In Ae,Kim, Youn Chul,Kim, Sung Yeon,Lee, Joo Young,Yun, Yong Gab,Park, Hyun John Wiley Sons, Ltd. 2009 Phytotherapy research Vol.23 No.12

<P>The aqueous extract of Mori Fructus (MF) exerts a change of phenotype and a cytoprotective effect in macrophages. The present study was carried out to investigate the immunomodulating activity of MF on the expression of nitric oxide (NO), tumor necrosis factor alpha (TNF-&agr;), co-stimulatory molecules and also interferon-gamma (IFN-&ggr;) in macrophages and splenocytes. Toll-like receptor 4 (TLR4) is a promising molecular target for immune-modulating drugs. It was hypothesized that one possible upstream signaling pathway leading to immunoregulation of MF may be mediated by TLRs. Multiple signaling molecules (NF-&kgr;B, ERK1/2, p38 and JNK) of the TLR4 signaling pathway were also detected. It was found that MF increased NO production and TNF-&agr; secretion in RAW 264.7 and peritoneal macrophages, co-stimulatory molecules expression in peritoneal macrophages and IFN-&ggr; expression in splenocytes. Further studies indicated that MF could significantly induce the phosphorylation of signal molecules of MAPKs and the degradation of I&kgr;B&agr; which finally led to the activation and nuclear translocation of nuclear factor-&kgr;B (NF-&kgr;B) for the target gene expression. All those notions disclosed that the aqueous extract MF is a new TLR4 activator, which induces a Th1 immune response as a consequence of induction of cytokines secretion, especially TNF-&agr; and IFN-&ggr;. Copyright © 2009 John Wiley & Sons, Ltd.</P>