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한국산 인삼의 Polyphenol 분획물의 항산화, Phospholipase $A_2$ 및 암세포증식 억제효과
최희진,한호석,박정혜,손준호,배종호,성태수,최청,Choi, Hee-Jin,Han, Ho-Suk,Park, Jung-Hye,Son, Jun-Ho,Bae, Jong-Ho,Seung, Tae-Su,Choi, Cheong 한국응용생명화학회 2003 한국농화학회지 Vol.46 No.3
한국산 인삼을 60% acetone으로 추출하여 Sepadex LH-20 gel column chromatography, MCI-CHP 20 gel column chromatography, ${\mu}-Bondapack\;C_{18}$ gel column chromatography을 이용하여 polyphenol 분획물을 3종류를 분리하여 항산화 효과, phospholipase $A_2$ 저해효과 및 암세포 증식억제 효과를 검토하였다. 항산화 효과에서 분획물 I은 150 ppm에서 $Cu^{2+},\;KO_2$ 그리고 $H_2O_2$에 대하여 각각 48.16%, 79.71%, 43.55%를 나타내었고 DPPH 수소공여능은 분획물 II가 200 ppm에서 35.17%의 라디칼소거능을 보였다. 분획물 III는 $Fe^{2+}$, OH가 존재 시 150 ppm에서 48.49%, 25%의 항산화효과를 나타내었다. Phospholipase $A_2$ 저해활성은 분획물 III이 $60\;{\mu}/ml$에서 48.9%의 활성을 나타냈다. HT 29 cell에 대한 인삼의 세포 독성은 분획물 II에서 0.25 mg/ml에서 73.29%의 가장 높은 억제능을 나타내었다. The polyphenol fractions of Korean ginseng were purified using Sephadex LH-20, MCI gel, Bondapak $C_{18}$ TLC, and HPLC from the 60% acetone soluble fraction. Fraction I showed 48.16%, 79.71% and 43.55% inhibition at 150 ppm against lipid oxidation in the presence of copper ion, superoxide and hydrogen peroxidation. Electron donating abilities of fraction II showed 35.17% inhibition at 200 ppm. Fraction III showed 48.49% and 25% inhibition at 150 ppm against lipid oxidation in the presence of ferrous ion and hydroxy radical ion. The phospholipase $A_2$ inhibitory effect of fraction III was 48.9% at the concentration of $60\;{\mu}g/ml$. The cytotoxic effects of fraction II was the highest (73.29% at 0.25 mg/ml) among the tested polyphenol fractions.
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송선영,이현화 한국피부과학연구원 2016 아시안뷰티화장품학술지 Vol.14 No.4
Purpose: This present study was performed to investigate anti-oxidant and anti-bacterial activities of Arecae pericarpium MeOH extracts and its solvent fractions. Methods: Arecae pericarpium was extracted by 80% MeOH and fractionated by n-hexane, ethyl acetate (EtOAc), n-butanol, and H2O. Free radical scavenging activities on 80% MeOH Arecae pericarpium extracts and its fractions were evaluated by using 1,1-diphenyl-2-picrylhydrazyl (DPPH) method. Anti-oxidant effects were confirmed by catalase (CAT) and superoxide dismutase (SOD) activities. Anti-bacterial activity was evaluated by the paper disk diffusion method. Results: Extraction yields of 80% MeOH Arecae pericarpium extracts and its fractions such as n-hexane, EtOAc, n-butanol, and H2O, were 19.04, 14.33, 12.07, 22.53, and 50.97%, respectively. The 80% MeOH extracts got the highest value (16.6 mg/g) in total polyphenol contents. In the case of DPPH free radical scavenging activity, the n-hexane fraction was 102.9 mg/mL. It was similar to the value of positive control, vitamin C and butylated hydroxytoluene (BHT). The highest values of SOD and CAT activities were confirmed at the EtOAc fraction (38.73 U mg protein-1) and the n-butanol fraction (2.75 U mg protein-1 min-1), respectively. In the results of anti-bacterial activity, 80% MeOH Arecae pericarpium extracts had anti-bacterial effects on all eight microorganisms. The n-hexane fraction had the anti-bacterial effects on only Listeria monocytogenes. There are no anti-bacterial effects on the other fractions such as EtOAc, n-butanol, and H2O. Conclusion: It is considered that these results imply the 80% MeOH Arecae pericarpium extracts can be used as not only natural antioxidants but also natural antibacterials. 목적: 본 연구는 대복피 메탄올(methanol, MeOH) 추출물 및 용매 분획물의 항산화 및 항균활성을 확인하고자 수행되었다. 방법: 대복피를 80% MeOH로 추출하고, n-hexane, ethyl acetate (EtOAc), n-butanol, H2O로 분획하였다. 추출물과 분획물의추출 수율, 총 폴리페놀 함량을 측정하였다. 대복피의 80% MeOH 추출물과 용매 분획물의 free radical 소거능은 1,1-diphenyl- 2-picrylhydrazyl (DPPH) 방법에 의해 평가하였다. 항산화 활성은 superoxide dismutase (SOD) 및 catalase (CAT) 활성으로 평가하였다. 항균활성은 디스크 확산법에 의해 평가하였다. 결과: 대복피 80% MeOH 추출물 및 n-hexane, EtOAc, n-butanol, H2O 용매 분획물의 추출 수율의 결과, 각각 19.04, 14.33, 12.07, 22.53, 50.97%로 측정되었다. 총 폴리페놀 함량은 80% MeOH 추출물에서 16.6 mg/g으로 가장 높게 측정되었다. DPPH free radical 소거능은 n-hexane 분획물에서 102.9 mg/mL로 양성대조군인 vitamin C 및 butylated hydroxytoluene (BHT)와 비슷한 우수한 항산화 활성을 나타내었다. SOD 활성은 EtOAc 분획물에서가장 높은 값(38.73 U mg protein-1)을 나타내었다. CAT 활성은 n-butanol 분획물에서 가장 높은 값(2.75 U mg protein-1 min-1) 을 나타내었다. 항균활성의 결과에서, 대복피 80% MeOH 추출물은 8종의 균주에 대해 항균활성을 나타냈다.- nHexane 분획물은오직 Listeria monocytogenes에 대해서만 항균활성을 나타냈다. EtOAc, n-butanol, H2O 분획물에서는 8종 균주에 대하여 항균활성을 나타내지 않았다. 결론: 이상의 결과에서 대복피 80% MeOH 추출물은 천연 항산화 및 항균 소재로서의 활용이 가능할 것으로 기대된다.
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한국산 인삼의 Polyphenol 분획물의 항산화, Phospholipase A2 및 암세포증식 억제효과
최희진 ( Choe Hui Jin ),한호석 ( Han Ho Seog ),박정혜 ( Park Jeong Hye ),손준호 ( Son Jun Ho ),배종호 ( Bae Jong Ho ),성태수 ( Seong Tae Su ),최청 ( Choe Cheong ) 한국응용생명화학회 2003 Applied Biological Chemistry (Appl Biol Chem) Vol.46 No.3
The polyphenol fractions of Korean ginseng were purified using Sephadex LH-20, MCI gel, Bondapak C_(18), TLC, and HPLC from the 60% acetone soluble fraction. Fraction I showed 48.16%, 79.71% and 43.55% inhibition at 150 ppm against lipid oxidation in the presence of copper ion, superoxide and hydrogen peroxidation. Electron donating abilities of fraction Ⅱ showed 35.17% inhibition at 200 ppm. Fraction Ⅲ showed 48.49% and 25% inhibition at 150 ppm against lipid oxidation in the presence of ferrous ion and hydroxy radical ion. The phospholipase A₂ inhibitory effect of fraction Ⅲ was 48.9% at the concentration of 60 ㎍/m/. The cytotoxic effects of fraction Ⅱ was the highest (73.29% at 0.25 ㎎/m/) among the tested polyphenol fractions.
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Neem 에탄올추출물과 분획물의 항산화, 항균 및 항염증 효과 비교
김미경(Mee-Kyung Kim) 한국인체미용예술학회 2021 한국인체미용예술학회지 Vol.22 No.1
In this study, the antioxidant, antimicrobial, and anti-inflammatory activities of a 70% ethanol extract of neem (Azadirachta indica) leafves and their fractions were evaluated to confirm the applicability as a functional cosmetic material. It was found that the total polyphenol content was higher in butanol (181.77±3.25 mg/g) and ethyl acetate fraction (180.27±1.53 mg/g) as compared to ethanol extract (139.16±1.04 mg/g). The electron donating ability and ABTS radical scavenging ability increased in a concentration-dependent manner in all samples. The IC50 value of electron donating ability was lower (357.6-375.1 ㎍/㎖) in ethyl acetate and butanol fractions compared to the than in ethanol extract (384.5 ㎍/㎖), and the ABTS radical scavenging activity was higher in the butanol fraction (66.8%) than the ethanol extract (64.3%) at the concentration of 1,000 ㎍/㎖. The antimicrobial activity of S. epidermidis and S. aureus were high in the ethyl acetate fraction, 8.5-11.5 mm and 8.5-15.0 mm, respectively, and the antibacterial activity against E. coli was high in the butanol fraction (8.5-14.0 mm). However, the antimicrobial activity against P. acne did not show a significant difference between the extract and the fraction. Cytotoxicity for RAW 264.7 cells was not observed up to the concentration of 100 ㎍/㎖ in both the extracts or fractions. The inhibition rate of NO production induced by LPS stimulation was highest in the ethyl acetate fraction (84.3%) than in the LPS alone treatment group, and about 2.3 times that of the ethanol extract (36.9%). Therefore, it was confirmed that the ethyl acetate fraction of neem leaves had a significant effect on the total polyphenol content, antioxidant, antibacterial activity, and inhibition of NO. In the future, it is considered necessary to study the anti-inflammatory mechanism of the ethyl acetate fraction.
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