위 아전절제술 후 십이지장 위 역류성 위염에서 p53 및 Ki-67 단백 발현 양상

최석채 ( Suck Chei Choi ),김용성 ( Yong Sung Kim ),김기훈 ( Ki Hoon Kim ),김헌수 ( Hun Soo Kim ),윤기중 ( Ki Jung Yun ) 대한소화기기능성질환·운동학회 2007 Journal of Neurogastroenterology and Motility (JNM Vol.13 No.2

목적 : 십이지장 위 역류성 위염은 위절제 등에 의해서 발생하는 것으로 후에 암종이 발생할 가능성이 높은 것으로 알려져 왔다. 그러나 사람에서 십이지장 내용물의 역류와 암종 발생 사이의 병인론적 연구가 흔하지 않다. 이에 십이지장 위 역류성 위염을 보이는 환자의 조직과 대조군 환자의 조직을 대상으로 Ki-67 및 p53 단백 발현 정도를 측정하여 상피 세포증식 유도 및 암억제 유전자의 발현 정도를 비교 연구하였다. 대상 및 방법 : 위암종으로 위아전절제술을 받은 총 57예 중 내시경 및 조직학적으로 십이지장 위 역류성 위염으로 진단된 16예의 내시경 조직과 대조군 16예의 내시경 조직을 대상으로 하여 Ki-67 및 p53 단백 발현을 면역조직화학적 염색을 하였고 그 강도를 점수화하였다. 결과 : 십이지장 위 역류성 위염 소견을 보인 환자의 내시경적 추적 기간은 평균 607일, 대조군 556일 그리고 57예의 평균은 471일로 유의한 차이는 없었다. 십이지장 위 역류성 위염 환자의 Ki-67 발현 강도의 중간값은 3.0로 대조군 중간값 2.0보다 의미있게 높았으며, p53 단백의 발현 강도 중간값도 2.0으로 대조군 중간값 1.0 보다 의미있게 높았다. 결론 : 십이지장 위 역류성 위염은 담즙 등이 점막의 상피세포 증식을 유도하고 유전자 이상을 초래할 가능성이 높아 시간 경과 후 암종이 발생할 가능성이 대조군에 비해서 높을 수 있음을 알 수 있었다. Background/Aims: Duodenogastric reflux of bile and other contents of duodenum is one of the main etiologic fators in chronic gastritis, and chronic inflammation has been recognized as a risk factor of human cancer. The aim of this study was therefore to evaluate the expression of p53 and Ki-67 protein in duodenogastric reflux gastritis. Methods: To evaluate the proliferation activity and tumor suppressor gene expression, 16 cases of duodenogastric reflux gastritis and 16 cases of control gastric tissue after subtotal gastrectomy were examined immunohistochemically using the monoclonal antibodies to Ki-67 and p53 protein. Results: The mean duration of follow-up endoscopic biopsy after subtotal gastrectomy was 607 days in duodenogastric reflux gastritis and 556 days in control groups. The mean intensity of Ki-67 in duodenogastric reflux gastritis was significantly higher than that of control tissues (3.0 vs 2.0). The mean intensity of p53 protein in duodenogastric reflux gastritis was significantly higher than that of control tissues (2.0 vs 1.0). Conclusions: The high expressions of Ki-67 and p53 protein in duodenogastric reflux gastritis may be one of the main mechanisms in the development of gastric stump carcinoma. (Kor J Neurogastroenterol Motil 2007;13:118-122)

Restoration Project of Heumgyeonggak-nu (欽敬閣漏) in the King Sejong Era

Sang Hyuk Kim,Yong-Hyun Yun2,Byeong-Hee Mihn,Seon Young Ham,Kyung Ha Lee,Dae-Sik Yoon,Go-Eun Choi,Ho-Chul Ki,Myung-Kyoon Yoon 한국천문학회 2017 天文學會報 Vol.42 No.2

성남시 12세 아동의 구강건강실태에 관한 조사연구

김기은 ( Ki Eun Kim ),신명미 ( Myung Mi Shin ),허성윤 ( Sung Yun Huh ) 한국치위생과학회 2005 치위생과학회지 Vol.5 No.1

초음파 영상 데이터를 활용한 내장기 추나요법 시술 부위 탐색 연구

이상진 ( Sang-jin Lee ),기성훈 ( Sung-hoon Ki ),고동균 ( Dong-kyun Koh ),이상훈 ( Sang-hun Lee ),임형호 ( Hyoung-ho Lim ),송윤경 ( Yun-kyung Song ) 한방재활의학과학회 2022 한방재활의학과학회지 Vol.32 No.2

Objectives This study was conducted to confirm anatomical information near the treatment areas of visceral chuna manual therapy and to secure stability and effectiveness during the treatment. Methods For 50 healthy adult men and women, a total of 13 ultrasound images were taken of the 7 treatment areas which are the representative treatment areas of visceral Chuna manual therapy. Results The treatment area of the bottom of the liver can be palpated around the right side ST19 and CV14. The treatment area of the gallbladder and the common bile duct can be palpated around the right side SP16 and ST20. The treatment area of the cardia and the pylorus can be palpated around the left side KI21, right side ST20, ST21, KI19, KI20, CV12, and CV13. The origin point of the mesentery root can be moved to the left and lower left from CV12 and can be palpated. The treatment area of the ileocecal valve and the cecum can be palpated around the right side SP14. The treatment area for the colic flexure can be palpated around the both side LR13. The treatment support point for the kidneys can be palpated around both side BL51. Conclusions It is thought that if the ultrasound image data from the meridians around the treatment site is used as an auxiliary, it will be more effective in terms of safety and effectiveness during the treatment of visceral Chuna manual therapy. (J Korean Med Rehabil 2022;32(2):139-154)

Focal adhesion kinase and src expression in premalignant and malignant skin lesions

( Ju Yeon Choi ),( Jae Yun Lim ),( Han Saem Kim ),( Jung Min ),( Jung In Kim ),( Hyun Min Seo ),( Sang Hyeon Hwang ),( Chong Won Choi ),( Yoon Hwan Kim ),( Jin Hee Sohn ),( Hyunjoo Lee ),( Won Serk Ki 대한피부과학회 2015 대한피부과학회 학술발표대회집 Vol.67 No.2

Background: Focal adhesion kinase (FAK) and Src are non-receptor tyrosine kinases. FAK and Src play a critical role in inducing malignant transformation in tumor cells. Objectives: We performed immunohistochemical staining for total and phosphorylated forms of FAK and Src, to evaluate the role of FAK and Src in the development of premalignant and malignant skin lesions. Methods: A total of 59 facial skin samples (30 actinic keratoses, 10 Bowen``s diseases, 13 squamous cell carcinomas and six perilesional skins) were immunohistochemically stained for Ki-67, total (t) and phosphorylated (p) form of FAK and Src. Methods: A total of 59 facial skin samples (30 actinic keratoses, 10 Bowen``s diseases, 13 squamous cell carcinomas and six perilesional skins) were immunohistochemically stained for Ki-67, total (t) and phosphorylated (p) form of FAK and Src. Results: Cells positive for t-Src, p-Src-y530, t-FAK and pFAK-s722 were detected in premalignant intra-epithelial lesions (PELs) and squamous cell carcinomas (SCCs), but not in the perilesional skin. There was a tendency towards high correlation between Ki-67 and t-FAK or pFAK-s722, suggestive of the active role of FAK in cell proliferation. Conclusion: Our findings of higher t-Src and p-Src-y530 positive cells in PELs, as compared to SCCs (with higher Ki-67 level), are suggestive of the other role of Src in tumor formation and progression, which requires further investigation.

식도의 원주상피 피복 점막에서 점액유전자 발현 및 세포증식능에 대한 연구

최석채 ( Suck Chei Choi ),김용성 ( Yong Sung Kim ),김기훈 ( Ki Hoon Kim ),김헌수 ( Hun Soo Kim ),조향정 ( Hyang Jeong Jo ),윤기중 ( Ki Jung Yun ) 대한소화기기능성질환·운동학회 2007 Journal of Neurogastroenterology and Motility (JNM Vol.13 No.1

목적 : 바렛식도는 지속적인 위식도역류 등으로 원위부 식도에 정상적으로 존재하는 편평상피세포 대신에 배상세포를 포함하는 장형 원주세포로 식도 점막이 피복되는 것을 말한다. 그리고 이형성을 거쳐 선암종으로 진행할 수 있기 때문에 이형성 이전 단계인 바렛식도의 발암과정에 대한 연구가 필요하다. 이에 바렛식도와 배상세포를 포함하지 않은 원주세포만 있는 식도를 대조군으로 하여 점액유전자 및 세포증식능에 대해 비교 연구하였다. 대상 및 방법 : 임상 및 내시경적으로 바렛식도가 의심되어 원위부 식도에서 생검한 환자들 중에서 배상세포가 있어 조직학적으로 바렛식도로 증명된 25명의 환자와 배상세포가 없었던 환자들 중에서 무작위로 선택한 30예를 대조군으로 하였다. 생검 당시의 나이와 성별 그리고 MUC1, MUC2, Ki-67에 대한 면역조직화학적 염색을 시행하였다. 결과 : 바렛식도의 평균 나이 및 남자 비율은 각각 65.3±10.1세, 76.0%이였고, 대조군의 평균 나이 및 남자 비율은 각각 53.0±14.8세, 60.0%로 바렛식도의 나이가 대조군식도보다 의의있게 높았다. MUC1은 바렛식도 및 대조군 모두에서 100% 발현되었고, MUC2 발현율은 바렛식도 및 대조군에서 각각 92%, 20%이었다. Ki-67 발현율은 바렛식도 및 대조군에서 각각 80.0%, 70.0%이였고, Ki-67 발현 강도의 평균은 바렛식도 1.20±0.76, 대조군 0.77±0.57로 발현 강도에서 바렛식도가 의의있게 높았다. 결론 : 바렛식도는 원주세포만 있는 식도에서 보다 좀더 지속적인 위식도역류 등의 자극으로 생긴다. 그리고 MUC2는 주로 바렛식도에서 발현되고 세포증식능은 바렛식도에서 좀더 높으며 이는 MUC2 발현과 관련될 수 있다고 생각된다. Background/Aims: Barrett`s esophagus is characterized by the presence of metaplastic columnar epithelium with goblet cells in the distal esophagus. Barrett`s esophagus progresses through low grade dysplasia and high grade dysplasia to adenocarcinoma. We studied the patient age, the mucin gene and the proliferation activity of biopsy-proven Barrett`s esophagus and simple columnar epithelium-lined esophagus. Methods: To evaluate the mucin gene expression and proliferation activity, twenty five cases of Barrett`s esophagus and thirty cases of control esophagus were examined immunohistochemically with using the monoclonal antibodies to MUC1, MUC2 and Ki-67. Results: The Barrett`s esophagus patients were older (mean: 65.3±10.1 years) than the control patients (mean: 53.0±14.8 years). The MUC1 expression was 100% in both Barrett`s esophagus and the control esophagus. An MUC2 expression was observed in 92.0% of the Barrett`s esophagus and 20.0% of the control esophagus. The rate and intensity of the Ki-67 expression was higher in the Barrett`s esophagus (80.0%, 1.20±0.76) than that in the control esophagus (70.0%, 0.77±0.57). Conclusions: Barrett`s esophagus is a metaplastic lesion due to the more long-standing gastroesophageal reflux than that in a simple columnar epithelium-lined esophagus. The cause of increased proliferation activity in Barrett`s esophagus may be related to the MUC2 expression. (Kor J Neurogastroenterol Motil 2007;13:21-25)

YMCA 체육의 역사적 함의

김영훈(Kim, Young-hun),장성호(Jang, Sung-Ho),박윤기(Park, Yun-Ki) 용인대학교 무도연구소 2012 武道硏究所誌 Vol.23 No.1

이 연구는 역대 YMCA 체육을 토대로 하여 구체적으로 살펴보고자 한다. 이에관해 생활체육 활성화 방안 모색을 제시하는데 논의 및 결론을 도출하였다. 이는 다음과 같이 확인할 수 있다. 첫째, 한국에서의 YMCA운동은 1899년 경성에서 선교활동을 시작되었다. 둘째, YMCA의 전인교육정신인 지(智)·덕(德)·체(體)의 정신과 통하는 정신으로서 비폭력, 민주주의의 실천교육과 상통하는 정신이었다. 셋째, 이상재의 교육사상은 인간의 본질적인 면에 치중하여 올바른 인격체로 양성하는 것으로 지·덕·체의 전인적 교육이었음 알 수 있었다. 넷째, 서울 YMCA의 창립과 역사적 배경을 보면 서울YMCA는 1903년 10월 28일 창립하였다. 다섯째, 서울YMCA를 도입, 회원들에게 지도한 스포츠로는 야구, 농구, 배구, 축구, 유도, 검도, 체조 등이 있었다. 이상과 같이 종합해 볼 때, 역대 YMCA 체육의 역사에서 보이듯이 그 정책을 통해 한국생활체육의 활성화를 위한 방안 모색에 따른 정체성을 제시할 수 있는 것이다. This research is looked at concretely is based on the generation of YMCA physical training. It derive a discussion and conclusion to grope a plan to activate living physical training. This can be confirmed by the following essay. First, the YMCA exercise in Korea started in Gyeong-sun, 1899 as a missionary activity. Second, the mind of former education of YMCA’s just·morality·formality was a mind connected to the communicating with anti-violence, democratic practice education. Third, Lee Sang-Jea’s educational method is to bring up the personality to the right side valued on true nature of a human being, which indicates that his educational method is for the whole man. Fourth, looking at the foundation and historical backround, Seoul YMCA was established on 28th October 1903. Fifth, sports that Seoul YMCA guided to members were baseball, basketball, volleyball, football, judo, kendo, gymnastics etc. To round up all the above statements, the identity following a plan to activate Korea living P.T can be derived through the policy as seen in YMCA physical training’s history in generations.

Comparative Studies on the Relations between Composition Ratio and Charge Transport of Diketopyrrolopyrrole-Based Random Copolymers

Yun, Hui-Jun,Cho, Jangwhan,Chung, Dae Sung,Kim, Yun-Hi,Kwon, Soon-Ki American Chemical Society 2014 Macromolecules Vol.47 No.20

<P>Random copolymerization strategy is introduced to increase the solubility of donor–acceptor copolymers so that they can be processed with environmentally benign, halogen-free solvents. Traditionally, it has been believed that the random copolymer with a lower crystalline order should have a significantly lower charge carrier mobility. This report shows that random copolymerization between two highly planar, charge-delocalized repeating units can significantly enhance the solubility while almost preserving the high charge carrier mobility. A comparative study was conducted for a series of random copolymers consisting of diketopyrrolopyrrole–thienothiophene (DPP-TT) and DPP–selenophene–vinylene–selenophene (DPP-SVS) with composition ratios of 10:0, 9:1, 7:3, 5:5, 3:7, 1:9, and 0:10 by using the crystalline order determined from grazing-incident X-ray diffraction measurements and charge carrier mobility determined from field effect transistor measurements. The results showed that the copolymer tends to have a lower degree of intermolecular ordering as the DPP-TT:DPP-SVS composition ratio approaches 5:5 because of the increased segmental randomness in the polymer chain. Nevertheless, the FET mobility did not follow the tendency of crystalline order; instead, it simply followed the order of increasing content of DPP-SVS in the copolymer. This result implies that we can use the random copolymerization strategy to increase the solubility of donor–acceptor copolymers if the two constituent repeating units are structurally planar and the electrons are fully delocalized.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/mamobx/2014/mamobx.2014.47.issue-20/ma501980m/production/images/medium/ma-2014-01980m_0003.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/ma501980m'>ACS Electronic Supporting Info</A></P>

Structural Integrity Evaluation of Spent Nuclear Fuel Assembly Under Normal Transportation Drop Conditions

Sang Soon Cho,Woo Seok Choi,Ki-Seog Seo,Yun-Young Yang 한국방사성폐기물학회 2017 한국방사성폐기물학회 학술논문요약집 Vol.2017 No.05

Effects of Carnosine on Exercise Capacity in ICR Mice

Ki-Nam Lee,Jin-Joo Hue,Myak Miga,Bong Su Kang,Jong-Soo Kim,Sang Yoon Nam,Young Won Yun,Jae-Hwang Jeong,Beom Jun Lee 한국실험동물학회 2008 Laboratory Animal Research Vol.24 No.4

Carnosine is a dipeptide (β-alanyl-L-histidine) found in mammalian skeletal muscle at high concentrations. Its biological functions in the muscle include antioxidant and buffering activities. The aim of this study was to evaluate the effect of carnosine on treadmill exercise capacity in mice. Fifty-six male ICR mice were divided into four groups including the control (vehicle) and oral carnosine-treated groups at the doses of 5, 50, and 500 ㎎/㎏/day for 4 weeks. All mice were acclimated to treadmill exercise for 4 weeks by running at 20-40 ㎝/sec for 10-30 min/day. At 2 and 4 weeks, endurance running time was measured. After 4 week, the activities of citrate synthase (CS), lactate dehydrogenase (LDH), and superoxide dismutase (SOD) were determined in gatrocnemius and soleus muscles. At 2 weeks after treatments, the endurance running time in the carnosine-treated groups (50 and 500 ㎎/㎏) was significantly shorter than those in the control group (P<0.05). However, there were no statistically differences in endurance running time at 4 weeks between the control and carnosine-treated groups. CS activity was not markedly different between the control and the carnosine-treated groups. LDH activity was significantly reduced in the carnosine-treated group (500 ㎎/㎏). SOD activity was also significantly reduced in the carnosinetreated groups (50 and 500 ㎎/㎏). These results indicate that carnosine may decrease the exercise capacity in certain physiological conditions.