위암과 식도암이 병발된 다발성 원발성 악성종양 1예

박사영,이나영,이효진,이선영,최진혁,이순남,심강섭,성순희,한운섭 梨花女子大學校 醫科大學 醫科學硏究所 1996 EMJ (Ewha medical journal) Vol.19 No.3

Multiple primary cancer means that more that two cancers occur independently in an individual. Recently, the incidence of multiple primary cancer has increased with lengthened survival, of cancer patients, development of new diagnostic technique and increased clinical evaluation. We report a patient who had adenocarcinoma of stomach combined with squamous cell carcinoma of esophagus simultaneously.

청감심리실험에 의한 판소리 홀의 음향평가

신영무,정사희 圓光大學校 1996 論文集 Vol.31 No.2

한국전통음악 중의 판소리는 거의 야외에서 공연되었으나, 현재는 실내에서 공연되는 경우가 더 많아졌다. 따라서, 판소리가 실내에서 공연되는 경우, 이 판소리가 실내에서는 어떤 청감적 인상을 나타내는지를 검토하기 위해 몇개의 항목(울림의 길이, 명료도 등)을 정하여 청감심리실험을 하였다. 본 연구에 참가한 피험자들의 분석결과는, 울림의 길이의 경우 판소리는 잔향시간이 0.6초에서 0.8초의 범위가, 명료도는 0.8초의 전후가, 전체적 인상은 1초전후의 범위가 좋은 홀의 조건으로써 제안할 수가 있게 되었다. 따라서, 이번의 청감실험에 의해 판소리의 경우는 아니리라고 하는 것이 공연중에 종종 튀어나오기 때문에, 아니리의 演行이 잘 되게 하기 위해서는 잔향시간이 짧은쪽의 홀이 좋은 명료도를 갖기 때문에, 잔향시간은 될 수 있는데로 짧은 쪽이 바람직하다는 것을 알게 되었다. 또한, 이번의 실험에 의해 임펄스 응답으로 부터는 모든 음향적 물리량 뿐만 아니라, 모든 청감평가를 위한 시험음을 만들 수가 있기 때문에, 이 음향적 신호처리기술의 응용은 실내음향설계에 있어서 매우 강한 도구로써 사용할 수 있다는 것이 검증되었다. In case PANSORI is playing indoor, for the purpose of its hearing impressions, we are investigated several items (length of sound reverberation, clearity etc.). Therefore, its result are length of sound reverberation is 0.6 sec. of reverberation time, clearity is 0.8 sec., total impressions of PANSORI is about 1.0 sec.. And if the using of this new indoor acoustic estimation technique, we are verifiable as follows, a) I founded that ground planning of Korean music hall is planed on the ground of special dramatical factors of Korean music and hall. b) Techniques of indoor acoustical design became to possible by the computer simulations of the building shape and reflected rays of sound and valuation of hearing test.

골프장의 잔디 토양에서 살균제 Tolclofosmethyl의 분해

정근욱,우선희,김흥태,사동민,김영기,홍순달,김재정,이재구 한국환경농학회 2004 한국환경농학회지 Vol.23 No.3

Tolclofosmethyl (TCFM) is heavily and annually applied to the turf soils of most golf courses in Gyeongju to control the fungi known to cause the disease brown patch. The soil samples used for the experiment was collected three weeks after the annual application at the end of May in the year 2002. The preliminary results obtained from this study demonstrated that the repeated field annual applications of TCFM to the turf soils of a golf course located in Gyeongju city in the southern area of Korea showed the enhanced degradation of the parent compound TCFM, especially in the surface (0~15 cm) soil rather than the shallow subsurface (15~30 cm) and deep subsurface (30~45 cm) soils, compared to the corresponding surface (0~15 cm) and shallow and deep subsurface (15~30 cm and 30~45 cm) soils of the untreated plot. It appears that microorganisms in the soil may be involved in the enhanced degradation of TCFM.

Identification of demyelination formation caused by JCvirus infection

Young-Hee Sa(사영희),Hyun Joo Kim(김현주),Bae Hwan Lee(이배환),Sun Joon Bae(배선준),Seong-Karp Hong(홍성갑) 한국정보통신학회 2020 한국정보통신학회 종합학술대회 논문집 Vol.24 No.1

JCvirus의 감염에 의해 유발되는 탈수초성 신경질환은 인간의신경계에서 다양한 신경의탈수초과정을 일으키며 통증을 동반하는 진행성 다병성 뇌병증 질환(Progressive multifocal encephalopathy (PML)) 등 많은 신경질환을 발생시킨다. 이러한 바이러스 감염반응은 인간의 신경세포의 탈수초화를 유발하여 신경세포의 파괴로 발생하는 병원성 기작으로 예상된다. 쥐의 배아 조직으로부터 슈반세포(Schwann cell)와 뉴런 세포(neuronal cell)를 각각 분리하여 각각 배양한 후에 두 세포군을 합쳐 공동배양을 하였고 몇주간 배양을 통해 수초(myelin)를 형성시켰다. 형성된 수초는 수초단백질인 MPZ(myelin protein zero), MBP(myelin basic protein)의 항체를 사용하여 western blot과 confocal microscope 등으로 분석하였다. 연구결과 JC 바이러스로 감염된 myelin 형성배양 세포들은 MPZ과 MBP 등 수초단백질이 전혀 발견되지 않았다. 따라서 myelin 형성배양 세포들은 JC 바이러스 감염에 의해 myelin 형성이 파괴된 것으로 확인되었다. 본 연구는 과학 기술부, ICT 및 미래 계획(NRF-2017R1A2B3005753)이 자금을 지원하는 국립 연구 재단(NRF)을 통한 기초 연구 프로그램과 연세의과대학교 교수연구 2019(CJ기업후원)의 지원을 받았다. Demyelination neuropathy caused by the infection of JCvirus causes various nerve demyelination processes in the human nervous system and causes many neurological diseases such as progressive multifocal encephalopathy (PML) accompanied by pain. This viral infection response is expected to be a pathogenic mechanism caused by the destruction of nerve cells by causing demyelination of nerve cells in humans. Schwann cells and neuronal cells were isolated from mouse embryonic tissues and cultured separately, and the two cell groups were combined and co-cultured. Cells co-cultured for some weeks formed myelin. Myelin formation was analyzed with western blot and confocal microscope using an antibody of myelin proteins, MPZ(myelin protein zero), MBP(myelin basic protein). From the result, Myelin-forming cells infected with JC virus were not found by MPZ and MBP. Therefore, we confirmed that myelin-forming cells were destroyed after JC virus infection. The study was supported by a Basic Research Program through the National Research Foundation (NRF) funded by the Ministry of Science and Technology, ICT and Future Plans (NRF-2017R1A2B3005753) and a faculty research grant of Yonsei University College of Medicine for 2016(6-2016-0161).

Induction of demyelination by of herpes simplex virus-1 in rat

Young-Hee Sa,Tae Dong Kweon,Ji-Young Kim,Bae Hwan Lee,Seong-Karp Hong 한국실험동물학회 2018 한국실험동물학회 학술발표대회 논문집 Vol.2018 No.1

Effects of Recombinant pOPINEneo-3C-GFP Vectors Containing Cancer Suppressor Genes

Young-Hee Sa(사영희),Chang-Shik Choi(최창식),Ki Hwan Lee (이기환),Seong-Karp Hong(홍성갑) 한국정보통신학회 2019 한국정보통신학회 종합학술대회 논문집 Vol.23 No.2

Baculovirus System은 곤충세포에서 단백질을 발현하는 viral system으로 baculovirus가 곤충세포에 감염되어 증폭되는 특성을 이용한 벡터시스템이다. 곤충세포는 대부분의 mammalian protein-targeting sequence를 인식하기 때문에 다양한 단백질을 발현시킬 수 있고, mammalian cell에서 수행되는 많은 post-translational modification이 일어난다는 장점이 있다. 베큘로 바이러스 시스템은 안전성, 대규모 및 높은 수준의 유전자 발현 관점에서 중요한 이점을 갖는다. 본 연구에서는 사이토 메갈로 바이러스 (CMV) 프로모터, 강화녹색 형광 단백질 (EGFP) 및 암억제유전자 p53 등이 포함된 pOPINEneo-3C-GFP 벡터를 재조합하였다. pOPINEneo-3C-GFP 재조합 벡터를 다양한 세포 및 세포주에 감염시키고 유전자의 전이 및 발현을 일반적인 벡터를 대조군으로 비교하여 분석하였다. 본 연구의 결과, pOPINEneo-3C-GFP 재조합 벡터가 유전자 전이 및 발현 면에서 대조군 벡터보다 더 높은 효율을 보여주었다. 본 연구는 과학 기술부, 한국 정보기술 진흥 기금 (MSIP)이 후원하는 한국 연구 재단 (NRF)을 통해 중견 연구원 프로그램 (NRF-2016R1A2B4016552)을 통해 지원되었다. Baculovirus System is a viral system that expresses proteins in insect cells. It is a vector system that uses baculovirus to infect and amplify insect cells. Since insect cells recognize most mammalian protein-targeting sequences, they can express a variety of proteins and have many post-translational modifications that occur in mammalian cells. The baculo virus system has important advantages in terms of safety, large scale and high levels of gene expression. In this study, pOPINEneo-3C-GFP vectors containing cytomegalovirus (CMV) promoter, enhanced green fluorescent protein (EGFP) and cancer suppressor gene p53 were recombined. The pOPINEneo-3C-GFP recombinant vector was infected with various cells and cell lines and the metastasis and expression of the genes were analyzed by comparing common vectors with controls. As a result of this study, pOPINEneo-3C-GFP recombinant vector showed higher efficiency in gene transfer and expression than control vector. This work was supported by a grant from Mid-Career Researcher Program (NRF-2016R1A2B4016552) through the National Research Foundation of Korea(NRF) funded by the Ministry of Science, ICT & Future Planning(MSIP).

Carbon Nanotubes/Heteroatom‐Doped Carbon Core–Sheath Nanostructures as Highly Active, Metal‐Free Oxygen Reduction Electrocatalysts for Alkaline Fuel Cells

Sa, Young Jin,Park, Chiyoung,Jeong, Hu Young,Park, Seok‐,Hee,Lee, Zonghoon,Kim, Kyoung Taek,Park, Gu‐,Gon,Joo, Sang Hoon WILEY‐VCH Verlag 2014 Angewandte Chemie Vol.126 No.16

<P><B>Abstract</B></P><P>A facile, scalable route to new nanocomposites that are based on carbon nanotubes/heteroatom‐doped carbon (CNT/HDC) core–sheath nanostructures is reported. These nanostructures were prepared by the adsorption of heteroatom‐containing ionic liquids on the walls of CNTs, followed by carbonization. The design of the CNT/HDC composite allows for combining the electrical conductivity of the CNTs with the catalytic activity of the heteroatom‐containing HDC sheath layers. The CNT/HDC nanostructures are highly active electrocatalysts for the oxygen reduction reaction and displayed one of the best performances among heteroatom‐doped nanocarbon catalysts in terms of half‐wave potential and kinetic current density. The four‐electron selectivity and the exchange current density of the CNT/HDC nanostructures are comparable with those of a Pt/C catalyst, and the CNT/HDC composites were superior to Pt/C in terms of long‐term durability and poison tolerance. Furthermore, an alkaline fuel cell that employs a CNT/HDC nanostructure as the cathode catalyst shows very high current and power densities, which sheds light on the practical applicability of these new nanocomposites.</P>

Myelin Formation by Coculture with Schwann Cells and Neuronal Cells and Myelin Degradation by JC Virus Infection

Young-Hee Sa(사영희),Hyun Joo Kim(김현주),Bae Hwan Lee(이배환),Sun Joon Bae(배선준),Seong-Karp Hong(홍성갑) 한국정보통신학회 2019 한국정보통신학회 종합학술대회 논문집 Vol.23 No.2

쥐의 배아 조직으로부터 슈반세포(Schwann cell)와 뉴런 세포(neuronal cell)를 각각 분리하여 각각 배양한 후에 두 세포군을 합쳐 공동배양하였다. 2주 동안 공동배양한 세포들은 myelin을 형성하였다. myelin 형성은 myelin 단백질인 myelin basic protein(MBP)의 항체를 사용하여 동초점 현미경으로 확인하였다. myelin 형성배양 세포들은 JC 바이러스를 3일 동안 감염시켰다. JC 바이러스 감염 후 myelin 형성배양 세포들은 MBP 항체를 사용하여 동초점 현미경으로 확인하였다. 그 결과 JC 바이러스 감염된 myelin 형성배양 세포들은 MBP가 거의 발견되지 않았다. 따라서 myelin 형성배양 세포들은 JC 바이러스에 의해 myelin 형성이 파괴 혹은 분해된 것으로 확인되었다. 본 연구는 과학 기술부, ICT 및 미래계획(NRF-2017R1A2B3005753)이 자금을 지원하는 국립 연구 재단 (NRF)을 통한 기초 연구 프로그램과 연세의과대학교 교수연구 2019(CJ기업후원)의 지원을 받았다. Schwann cells and neuronal cells were isolated from mouse embryonic tissues and cultured separately, and the two cell groups were combined and co-cultured. Cells co-cultured for 2 weeks formed myelin. Myelin formation was confirmed by confocal microscopy using an antibody of myelin protein, myelin basic protein (MBP). Myelin-forming cells were infected with JC virus for 3 days. Myelin-forming cells after JC virus infection were identified by confocal microscopy using MBP antibody. As a result, myelin-forming cells infected with JC virus had almost no MBP. Therefore, myelin-forming cells were found to be destroyed or degraded by the JC virus. The study was supported by a Basic Research Program through the National Research Foundation (NRF) funded by the Ministry of Science and Technology, ICT and Future Plans (NRF-2017R1A2B3005753) and a faculty research grant of Yonsei University College of Medicine for 2016(6-2016-0161).

Expression of recombinant baculoviral vectors in tumor cells and tissues

Young-Hee Sa,Chang-Shik Choi,Ki Hwan Lee,Seong-Karp Hong 한국실험동물학회 2018 한국실험동물학회 학술발표대회 논문집 Vol.2018 No.1

Management with Leukotriene-Receptor Antagonist in a Dog with Allergic Bronchitis

Young Kang Ho,Young Sung Nam,Sa Hee Min,Ji Hwan Park,Tae Yeon Hahn,Tae Woo Kim,Soo Yeon Jeong,Hee Myung Park 대한수의학회 2012 대한수의학회 학술대회발표집 Vol.2012 No.-