가축분뇨를 이용한 SCP 생산 균주의 분리 및 균체 단백질 생산

한석균,고유석,안태영,배동훈 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.6

질소원으로서 계분을 이용하는 균주를 선별하고 계분배지에서 균체의 생육속도가 다른 균주에 비하여 우수한 균주를 분리하였다. 형태·생리학적 특성을 기초로 하여 yeast의 분류 기준과 비교하여 본 균주를 Candida sp.로 동정하였으며 본 균주를 Candida sp. D116으로 명명하였다. Poultry feces extract medium에서 4% 농도의 glucose 첨가가 균체 생육에 효과적이었다. D116 균주를 액체 발효하여 균체생산능, 요산 그리고 가용성 단백질의 변화를 조사하였다. 그 결과 약 60시간이 경과하면 액체 발효 배지내의 거의 모든 가용성 단백질 및 요산의 감소를 보였으며 균체생육은 약 36시간 배양하였을 때 최고조에 도달하였고 그 후에는 점차 감소하는 경향을 보였다. SCP의 대량생산의 결과 50%의 계분혼합 배지와 30℃의 배양 온도에서 36시간 배양하여 균주의 생육수준이 3.8×10^9 CFU/ml 농도의 균체를 생산하였고 200 L의 배양액중 약 870 g-dw의 균체를 얻었으며 생산된 군체의 조단백질 함량은 67%이었다. Production of Single Cell Protein from Poultry Feces. Suk-kyun Han, You-Suk Go, Tae-Young Ahn and Dong-Hoon Bal^1*. Deparment of Microbioligy, College of Natural Sciences, Dankook Univerity, Cheonan 330-714 and Research Center for Molecular Microbiology, Seoul Nationa University, Seoul 151-742, Korea, ^1Department of Food Engineering. College of Engineering, Dankook University, Cheonan 330-714 and Bioproducts Research Center of Yonsei University, Seoul 120-749, Korea - From the soil collected form provincial area of South Korea, a microorganisms which have been shown good growth in the minimal poultry feces extract medium was isolated. Supplement of glucose to the poultry feces extract medium helped the complete degraded during the microbial growth. Maximum cell growth (3.8×10^9 CFU/ml) obtained at 36 hours of incubation after inoculation. Uric acid was degraded faster in minimal medium that in the glucose complement medium. VFA (volatile fatty acid), which are known as major compounds of poultry feces odor, were almost removed from the minimal poultry feces extract medium. Glucose supplement to the minimal medium enhanced the growth of microbial cells. Addition of 4% of glucose and 4% of neopeptone to the minimal poultry feces extract medium helped the maximal growth of cells.

漢江下流部 水害輕減을 爲한 調査 硏究

金漢星,高在雄,李相鎔,玄義泰,柳興根 건국대학교 1974 學術誌 Vol.17 No.1

The effect of reducing flood magnitudes, flood stages, and flood damage by upstream reservoir regulation are investigated in this study. The analytical procedures which has been established newly, are treated through this study for 7 index stations and same numbr of reaches. The method of this study could be expressed briefly as following procedures. Using stage-frequency relations and the potential damage survey data, damage-frequency relations are established, and from this, average annual flood damages computed. By holding out portions of the floods with various amounts of flood control storage in 6 reservoirs in the basin, routing were made to establish modified stage-frequency relations. From damage frequency relation, average annual damages are computed with and without condition of each control volume. The study result are concluded as follows; (1) The relationtship of corresponding water level between nearby stations are useful tools to adjust error of the data due to observation error and change of the gaging location. (2) The number of records about 10 years could not be applied for the statistical methods of flood frequency analysis. (3) The regional skew factors are recommended to apply flood frequency study by the log-Pearson Type III method. (4) The effect of damage reductions are greatly affectedin accordance with the routed flood. Therefore application of the Typical Tributary Contribution Flood are reasonable for this type of study. (5) In the North Han, effect of damage reduction due to the lacation of control point are gradually increased from upstream to downward until the confluence of North and South Han. (6) In the South Han, control effect are increased from Chungju to Yeoju where the maximum effect are analyzed and then decrease gradually until the confluence. (7) Better hydrologic and topographic combinations for the flood control are existed in South Han to compare with North Han in this study results.

인터넷중독에 대한 국내 인지행동치료 프로그램의 연구동향 고찰

한인화, 유영달 신라대학교 (부산여자대학교) 2015 論文集 Vol.60 No.-

노인 인구에서 신경인지기능저하와 혈청 지질농도와의 상관성

유영선,최석주,정성수,김지은,윤성욱,전동욱,백준형,박성우,이정구,추일한,김영훈 大韓神經精神醫學會 2008 신경정신의학 Vol.47 No.6

Objectives : In this study, the authors evaluated the correlation between eurocognitive impairments and serum lipids levels among Korean elderly over the age of 65. Methods : A total of 609 elderly individuals participated in this study. Screening for cognitive impairments were carried out using the Mini-Mental Status Examination-Korean version (MMSE-KC). There were 197 subjects above 1.5SD Of MMSE- KC and they were evaluated using the Korean versions of the Consortium to Establish a Registry for Alzheimer's Disease (AD) (CERAD-KC) and Geriatric Depression Scale (GDS-K). Results : There was a correlation between old age/low levels of education and AD (p<0.05, P<0.01). There was an inverse correlation between the serum level of total cholesterol and the word list recognition test scores. The serum levels of LDL cholesterol were inversely correlated with the scores on the constructional praxis test, word list recall test and word list recognition test. Inverse correlations between the serum level of triglyceride and scores on the word list recall test and word list recognition test were also observed. Conclusion : There were inverse correlations between the serum levels of lipids and language/memory function in subjects with AD.

활성화 C 단백 내성검사의 정도관리와 한국인의 양성률

이영경,박성섭,이유경,이진숙,함천경,주세익,윤근영,박선양,조한익 대한임상병리학회 1996 대한임상병리학회지 Vol.16 No.6

첫 삽화 정신분열병의 전구증상과 치료 전 경로 : 한국과 중국 연변간의 횡 문화 예비연구 Preliminary transcultural Study between Korea and China

정영철,박태원,유일한,현길용 대한정신분열병학회 2003 정신분열병 클리닉 Vol.6 No.2

차체 점용접 접합부 피로강도의 실험해석

김정한,유희,염영진,주영우,주석재 울산대학교 1999 공학연구논문집 Vol.30 No.1

다양한 외부 동하중에 의한 피로파단이 발생하고 있는 승용차 차체 점용접 접합부에 대해 피로시험을 수행함으로써 전반적이 피로거동을 파악하고자 하였다. 실제 파단 발생 부재를 구성하는 차체재료중 SPCC, SPRC35, SAPH38P를 선택하여 용접조건, 동종 및 이종재료간의 접합, 두께 조합을 가급적 실차와 동일하게 적용하여 다양한 인장-전단 및 십자-인장 점용접 시편을 제작하였다. 그리고 정적시험 및 피로시험을 수행하여 강도 데이터베이스를 구축하였다. The fatigue behaviour of spot-welded joints of a car body, in which fatigue fracture occurs by various external dynamic loads, is characterized by performing fatigue tests. SPCC, SPRC35, and SAPH38P steels are selected which constitute the car body. Welding conditions and combinations of steels and thicknesses are chosen to reproduce those of a real car body and various tensile-shear and cross-tension spot-welded specimens are manufactured. Both static tests and fatigue tests are carried out, and the database of spot-weld strength is constructed.

저압 증발하의 열교환 특성에 관한 연구

신유식,이윤환,서종수,전영흥,정효민,정한식 경상대학교 해양산업연구소 2002 해양산업연구소보 Vol.15 No.-

The purpose of this research is to obtain the characteristics of heat transfer or heat exchange under the vacuum pressure. The general heat transfer or heat exchange can be changed with the experimental environment. In this report, the exothermic calorie and endothermic calorie were measured under the vacuum chamber. These two kinds of calories show the evaporative and condensing sides, respectively, and these calories were measured by using the parameter with flow rate.

건설공사의 재시공 현황에 대한 원인분석 및 개선안에 관한 연구 : 공동주택의 공조공사를 중심으로 한 조사 및 사례연구

이기한,유철종,김용수 중앙대학교 건설환경연구소 2000 環境科學硏究 Vol.11 No.2

This study aims to provide some efficient and effective ways to prevent reconstruction. For this study, the analysis on the current situation and various causes of reconstruction has been performed by using questionnaire survey and case studies from construction sites. The results of this study are as follows: 1.Causes of reconstruction occurrence through case studies show construction inability including technician' idleness(42.2%), misunderstanding of specification(26.7%) and imperfect specification(15.6%). 2.Influence of reconstruction on construction productivity is analysed as the following; increase of 3.8% in construction duration, increase of 5.7% in construction cost, and decrease of 4.4% in construction quality. 3.In order to prevent reconstruction, it is required to file and analyse reconstruction data, systemize construction management, train construction worders with systematic work procedural manual, and check design documents precisely. 4.Also it is necessary to develop new construction technology such as simplification, mechanization, dry-construction of work process.

지속적인 직류 자장이 백서의 임의형 피판생존에 미치는 영향에 關한 硏究

백세민,유영준,한흥수,오갑성 大韓成形外科學會 1994 Archives of Plastic Surgery Vol.21 No.3

The present study was undertaken to determine if direct current(DC) electirical fields could improve survival rate of random pattern skin flap in the rat. 20 Sparque- Dawley rats were divided into two groups and caudally based 2cm width and 8cm length random pattern flaps were elevated in all rats. Electrical fields of 9mA were introduced by 1.5V disk button battery. Electrical field was applied for 5days to the stimulation group(B). DC electrical fields shows the enhancement of flap survival by increasing the rate to 33.5%(p<0.01). The enhancement of skin flap survival by application of direct current electrical field has possibility for cover the large skin defect.