노화흰쥐의 해마에서 parvalbumin과 calbindin-D28K의 면역반응세포에 관한 연구

정윤영,김종중 朝鮮大學校 附設 醫學硏究所 2009 The Medical Journal of Chosun University Vol.33 No.S

Aging of the hippocampus has been characterized by generative changes of fiber connetions, cell loss, and involved memory and learning impariment, cognition deficit. Aging leads to alternation in activity of parval-bumin (PV) and calbindin-D28k(CB) expressing in the brain of the rats. Two of calcium binding proteins (CBPs), PV and CB are, widely expressed in the adult central nuervous system. The aim of the present study is, therefore, to investigative possible changes in the number and morphology of PV and CB-immunoreactive neurons with aging in each area of the hippocampus of aged rats. The number and density of PV-immunoreactive neurons in the dendate gyrus and CA1 subregion was decreased in the hippocampus in aged group when compared with the young group. But, the number of PV-immunoreactive neurons in the CA3 subregion of the aged group was similar to that in the young group. And, excepting the CA1 subregion, the number of CB-immunoreactive neurons was not practically different in the young group when compared with the aged group. These results showed the alterative expression of the two CBPs during aging process of hippocampus. The age-related reduction of CBPs in hippocampus subregion is likely to deprive neurons from the capacity to better intracellular calcium and to leave them vulnerable to pathological processes.

현행 중등학교 과학 실험 , 실습 교육 실태 조사 및 그 운영 진단 (Ⅱ) : 고등학교 과학 실험 , 실습 교육을 중심으로

김영호,이윤종,정원우,강용희,양승영,윤성효,안병호,윤일희,권용주,임성규,오철한,기우항,전명남,김중욱 한국과학교육학회 1998 한국과학교육학회지 Vol.18 No.3

본 연구는 96년도에 실시한 우리 나라 중학교 과학 실험·실습 교육의 실태 조사 연구에 따른 계속 연구의 일환으로 고등학교 과학 실험·실습 교육의 실태를 조사하기 위한 것이다. 연구내용은 고등학교 과학 교과서의 분석, 실험·실습 실시 현황 분석, 현행 실험·실습 교육의 문제점 분석, 현행 학교 구성원들의 실험·실습 교육 운영 실태 조사 및 현행 실험·실습 교육의 개선을 위한 연구과제 도출을 주요 연구내용으로 다루었다. 연구방법은 전국의 고등학교 80개교를 대상으로 이들 학교의 학생 1,977명, 교사 165명, 학교장 80명에게 적용하였다. 본 연구에서 밝혀진 결과를 중심으로 우리 나라 고등학교 과학 실험 실습 교육의 정상화를 위한 실험 내적인 연구과제를 제시하면 다음과 같다. (1)교재 개발에 대한 연구, (2)실험 킷트 개발에 대한 연구, (3)교사교육 및 재교육 프로그램 개발에 대한 연구, (4)교실 밖 실험·실습 활동 프로그램 개발에 대한 연구, (5)과학 실험·실습 소재의 개발에 대한 연구. This study was accomplished to analysis and survey on the experimental and practical science education of high school in korea for the consecutive study of the an analysis and survey on the experimental and practical science education of middle school in korea(Lee, Yoon-Jong et al., 1997). The status of facilities, management for the experiment, practices, teaching methods in high schools have been investigated. The present status and reasonable management of the high school science education have been grasped from the questionaires. To do this 165 high school science teachers, 1977 students and 80 principals of high schools in Korea are administered questionaires of Science Education Research Institute of Kyungpook National University(1997). The results of this study are as follows : The reasonable management for experiments and practices of science education were scanty in the high school around the urban and rural school owing to the shortage of facilities and equipments, crowded class, excessive class works for teacher, excessive contents of present textbooks and insufficiency of the administrative supports etc. The current teaching method of high school science has emphasized knowledge. This fact does not satisfy the objective of learning due to lack of the teaching method. Desirable directions for the improvement of present status of high school science education were proposed in this paper.

척수를 손상시킨 후 꼬리정맥에 주입한 사람탯줄혈액세포가 뇌줄기에 미치는 영향

김종중,정윤영,박영란,문영민,현영식,정영욱,문정석 朝鮮大學校 附設 醫學硏究所 2007 The Medical Journal of Chosun University Vol.32 No.1

Background and Objectives: Stem cells are a valuable resource for treatment of many disease, but limited access to stem cells in some organs such as brain restricts their utility. Many approaches have been attempted to restore the function following brain stem injury (BSI) and spinal cord injury (SCI). The use of the human umbilical cord blood cells (hUCB) - a rich source of nonembryonic or adult stem cells - has recently been reported to ameliorate the behavioral consequences of stroke. Mateiials and Methods: Forty rats were divided into 8 groups: (1) SCI l+hUCB (infused 1 day post injury); (2) SCI 2+hUCB (infused 2 days post injury); (3) SCI 3+hUCB (infused 3 days post injury); (4) SCI 4+hUCB (infused 4 days post injury); (5) SCI 5+hUCB (mfusedt 5 days post injury); (6) SCI 6+hUCB (infused 6 days post injury); (7) LO+hUCB (laminectomy+hUCB); and (8) LO (laminectomy only). SCI was produced by compressing the spinal cord for one minute with an aneurysm clip calibrated to a closing pressure of 50 g. We report here that immunhistotochemical identification of fluorescent hUCB positive cells in the brain stem after compressed spinal cord injury using mouse anti-human mitochondria monoclonal antibody (MAB1273). Results: All SCI+hUCB (1-8) groups contained fluorescent hUCB positive cells in the all area of the brain stem. But especially a large number of fluorescent hUCB positive cells were observed in the whole area of the brain stem of the experimental 5 (SCI 5+hUCB) and 6 (SCI 6+hUCB)groups. No hUCB positive cells were found in the brain stem of group with non-injured spinal cord of these animals and group with laminectomy only. Conclusion: These results suggest that hUCB are potentially useful as a vector for treating a variety of the central nervous system disorders, and we are sure that continuous of stem cell study will give an best opportunity to treat the uncurable disorders in the future.

흰쥐 시상에서 Epidermal growth factor receptor면역반응 신경세포의 생후 발달에 관한 연구

박영란,정윤영,김종중,문정석,오재욱,정영욱,김주수 조선대학교 2003 The Medical Journal of Chosun University Vol.28 No.1

Background and Objectives : Epidermal growth factor receptor (EGFR), a 170-kDa transmembrane glycoprotein, appears to mediate epidermal growth factor (EGF) activity. Transforming growth factor-α and EGF produce their biological effects in numerous systems by stimulating the EGFR In this study, we examine the postnatal development of EGFR immunoreactivity in the different regions of the thalamus of the rat Materials and Methods : The present study is based on 28 postnatal cases of rat thalamus ranging from the day of birth, postnatal day 0 (P0) to 30 days (P3, P5, P10, P15, P20, P30), and these cases were compared with adult rat thalamus. Cryostat sections were processed free-floating with monoclonal antibody by immunohistochemistry Results : EGFR immunoreactivity in the thalamus of the rat showed very different patterns according to postnatal ages and thalamic areas. EGFR-immunoreactive cells appeared in the first two postnatal weeks, except the ventral posterior thalamic nuclei. In the early postnatal days, EGFR-immunoreactive cells appeared thalamic midline structures, increased progressively in the first two postnatal weeks, and followed mediolateral gradient. The mature patterns of EGFR-immunoreactive cells were achieved at P20 Conclusion : These data indicate that the maturation of EGFR-immunoreactive cells requires a relatively prolonged period of time to achieve an adult configuration. Many growth factors probably play protective or neurotrophic roles at EGFR-immunoreactive neurons of thalamus both young and adult rats In addition to difference in time of appearance in thalamic nuclei and developing pattern with mediolateral gradient suggest that EGFR-immunoreactivities are correlated with the appearance of the related functional.

흰쥐 올리브소뇌계통의 발생에 따른 IGF-I에 대한 면역조직화학적 연구

장인엽,윤상필,정윤영,김종중,문정석,김영택,장영훈 대한해부학회 1999 Anatomy & Cell Biology Vol.32 No.4

複合 韓藥劑 淸籬滋坎湯의 抗癌效果 및 作用機轉 糾明

Cho, Kyung-Sam,Kim, Si-Young,Park, Jai-Kyung,Choi, Seung-Hoon,Chung, Se-Young,Yoon, Hwi-Joong 경희대학교 동서의학연구소 1999 INTERNATIONAL SYMPOSIUM ON EAST-WEST MEDICINE Vol.1999 No.1

Kyung Sam Cho¹Si Young Kim¹, Jai Kyung Park²,Seung Hoon Choi³,Se Young Chung⁴, Hwi Joong Yoon¹¹College of Medicine, ²East-West Medical Research Institute, ³College of Oriental Medicine, ⁴College of Pharmacology, Kyung Hee University,Seoul, Korea. The Anti-cancer Effect of Oriental Medicine Chung-Ri-Ja-Gam-Tang in the Mouse with Metastatic Lung Cancer. Proceedings of International Symposium on East-West Medicine, Seoul. 231-243, 1999.-The oriental medicine Chung-Ri-Ja-Gam-Tang is an herbal medicine which has been used for pulmonary diseases and known as having immune stimulatory effects. It has been known effective in lung cancer. So we studied the effects and the mechanisms of this herbal medicine it the mouse with metastatic lung cancer. The metastatic lung cancer of the mouse was produced with melnoma cell line(B16BL/6).5×10□/mouse tumor cells were injected intravenously to the CDF1 mouse via tail vein. The mice were divided 4 groups. The first group was treated with 50mg/kg extract of Chung-Ri-Ja-Gam-Tang per oral for 10 days after cancer cell injection, second group treated with saline after cancer cell injection, the third group with medicine without cancer cell, and the last group with saline only. After 10 days treatments some of the mice were scarificed and the lung and spleen was removed. The survival duration, weight change, the number of metastatic cancer nodule of the lung, the NK cell activity, the capacity of cytokines(INF-γ,INF-α,IL-2) production and the proliferation activities of mouse lymphocytes were measured. The surival times of the group 1 mice were longer and the weight loss was less than the group 2 significantly. The number of the metastatic nodule of the lung were decresed in the group 1 than the group 2. The production of INF-γ,was increase in group 2 than group 3 and 4, IL-2 production was increased in group 1 than group 2,3,4 significantly. There was no difference in TNF- α production and proliferation activity of lymphocyte in each group. The NK cell activity was significantly increased in group 1 than group 2,3,4. We conclude that the Chung-Ri-Ja-Gam-Tang has the effect of increasing the NK cell activity of the CDF1 mouse with metastatic lung cancer(B16BL/6). And it is suggested that the increased production of the IL-2 is the mechanisms of enhanced NK cell activity.

모체 Thyroxine 투여가 태아알코올효과를 가진 흰쥐 대뇌겉질 및 해마에서 NPY함유 신경세포의 생후 발달에 미치는 영향

김복,박상기,박영란,김종중,문정석,김주수,문영민,현영식,천관영,정윤영 朝鮮大學校 附設 醫學硏究所 2005 The Medical Journal of Chosun University Vol.30 No.2

Background and Objectives: Maternal alcohol abuse is considered to be one of the most prominent cause of neurobiological malformations in the postnatal and adult life of the offspring. In this study, we investigated the effects of maternal alcohol drinking on the postnatal development of NPY-containing neuron, and, the influence of thyroxine treatment on the cerebral cortex and hippocampus of pups of alcohol abused mother. Materials and Method: Time-pregnant rats were divided into three groups. Alcohol-fed group A received 35 calories of liquid alcohol diet daily from gestation day 6; control pair-fed group B was fed a liquid diet in dextrin replaced alcohol isocalorically: alcohol + T4 group C received 35 calories liquid alcohol diet and exogenous thyroxine subcutaneously. Results: Group C showed prominent NPY immunoreactivity in the cerebral cortex compared to group A and B at P7. In group C, NPY-containing neurons were widely distributed in the all layers of cerebral cortex after P14. Besides, numerical decrease of NPY-containing neuron as age increases was not found in group C. However, the decrease of NPY-containing neuron was clearly observed in group A compared to group C after P14. In hippocampus, group Band C were appeared similar patterns after P7. Additionally, in group C, NPY immunoreactivity was prominently appeared in CA2 and CA3 at P14 as compared to group B. Conclusions: The present results showed the increase of intensity and number of NPY-containing neurons in cerebral cortex and hippocampus of pups of exogenous T₄ supplemented alcohol-exposed dams as compared to control pair-fed and alcohol-exposed pups at P7. It presumably suggest that NPY-containing neurons might be regulated by the early postnatal growth stimulatory effect of the exogenously supplemented T₄. Therefore, the increase of NPY synthesis caused by maternal administration of exogenous thyroxine may ameliorate fetal alcohol effect, one of the ill effects as a result of the dysthyroid state following maternal alcohol abuse.

여성 노인을 대상으로 한 분노조절 프로그램이 부정적 기분 개선에 미치는 효과

윤은영,박중규 대구대학교 특수교육재활과학연구소 2008 再活科學硏究 Vol.26 No.2

여성 노인을 대상으로 분노조절 프로그램을 활용하여 부정적 기분 개선에 미치는 효과를 검증하고자 하였다. 실험집단 4명, 통제집단 3명을 대상으로 프로그램 적용 전․후에 특성분노, 분노표현, 우울, 자아존중감 수준을 측정하여, 프로그램 사후에 각 측정영역별로 반복측정 변량분석을 실시하였다. 프로그램은 주 1회, 90분 씩 10주 동안 총 10회기로 진행되었으며, 내용은 회상요법, 이완적 요소, 인지적 요소 등을 적용하였고, 참가 노인의 동기유발, 적극적인 참여에 중점을 두어 실시하였다. 분노조절 프로그램은 특성분노와 분노억제 수준을 감소시키고 분노표출 수준은 증가시켰으나, 분노통제 수준에서는 유의미한 차이가 나타나지 않았다. 또한 분노조절 프로그램은 우울 성향을 감소시켰고 자아 존중감을 향상시켰다. 이러한 결과로 보건데 여성 노인을 대상으로 한 분노조절 프로그램은 분노, 우울, 자아존중감에 영향을 미치며, 여성 노인의 부정적 기분을 개선시키는데 효과적이라고 판단할 수 있었다. This study was conducted to prove the effect of an anger management program on the improvement of negative mood in elderly women by utilizing the program for elderly women, and identify implications in the execution of the program. In order to prove the effect of the execution of the program, the study aimed at 4 participants in a test group and 3 participants in a control group, and measured their trait anger, anger ventilation, depression, and self-esteem before and after the application of the program. Then, the study conducted a pre-and post analysis of variance with repeated measures by the scale of each measured area, and the program was given once a week, 90 minutes per session, for 10 weeks, totaling 10 sessions. The program was delivered through the application of reminiscence therapy, a relaxation factor, and a cognitive factor, and it was focused on motivation and active participation. The research results include the following. 1) The anger management program decreased the trait anger and anger suppression levels, and increased the anger ventilation level; however, there was no significant difference in the anger control level. 2) The anger management program diminished a depressive tendency, and improved self-esteem. Therefore, the anger management program aimed at elderly women was found effective for improving the negative mood of elderly women, while affecting their anger, depression, and self-esteem. 여성 노인을 대상으로 분노조절 프로그램을 활용하여 부정적 기분 개선에 미치는 효과를 검증하고자 하였다. 실험집단 4명, 통제집단 3명을 대상으로 프로그램 적용 전․후에 특성분노, 분노표현, 우울, 자아존중감 수준을 측정하여, 프로그램 사후에 각 측정영역별로 반복측정 변량분석을 실시하였다. 프로그램은 주 1회, 90분 씩 10주 동안 총 10회기로 진행되었으며, 내용은 회상요법, 이완적 요소, 인지적 요소 등을 적용하였고, 참가 노인의 동기유발, 적극적인 참여에 중점을 두어 실시하였다. 분노조절 프로그램은 특성분노와 분노억제 수준을 감소시키고 분노표출 수준은 증가시켰으나, 분노통제 수준에서는 유의미한 차이가 나타나지 않았다. 또한 분노조절 프로그램은 우울 성향을 감소시켰고 자아 존중감을 향상시켰다. 이러한 결과로 보건데 여성 노인을 대상으로 한 분노조절 프로그램은 분노, 우울, 자아존중감에 영향을 미치며, 여성 노인의 부정적 기분을 개선시키는데 효과적이라고 판단할 수 있었다. This study was conducted to prove the effect of an anger management program on the improvement of negative mood in elderly women by utilizing the program for elderly women, and identify implications in the execution of the program. In order to prove the effect of the execution of the program, the study aimed at 4 participants in a test group and 3 participants in a control group, and measured their trait anger, anger ventilation, depression, and self-esteem before and after the application of the program. Then, the study conducted a pre-and post analysis of variance with repeated measures by the scale of each measured area, and the program was given once a week, 90 minutes per session, for 10 weeks, totaling 10 sessions. The program was delivered through the application of reminiscence therapy, a relaxation factor, and a cognitive factor, and it was focused on motivation and active participation. The research results include the following. 1) The anger management program decreased the trait anger and anger suppression levels, and increased the anger ventilation level; however, there was no significant difference in the anger control level. 2) The anger management program diminished a depressive tendency, and improved self-esteem. Therefore, the anger management program aimed at elderly women was found effective for improving the negative mood of elderly women, while affecting their anger, depression, and self-esteem.

충남대학교 기초과학분야의 효율적 육성에 관한 연구

윤화중,진성일,윤민중,윤혜수,이영하,최철규,이천배,신대현 충남대학교 자연과학연구소 1986 忠南科學硏究誌 Vol.13 No.2

Chungnam National University (CNU) is located in Daeduk Science Town, a center of science and technology in Korea, and it is expected to play an important role in the development of science in Korea. With this in mind, the basic natural sciences at CNU should be more actively supported in both education and research. Accordingly analysis and comparison of the basic science program at major college in Korea as well as those in the USA and Europe has been performed. The current cooperative relationship between CNU and research institutes in the science town has also been analyzed. This study has resulted in propositions to make more efficient the upbringing of the basic sciences at CNU, by focusing on the following points ; a) to improve academic affairs, b) to make graduate studies substantial, c) to secure highly qualified faculty members, d) to increase cooperation in research between CNU and reserch institutes, e) to enlarge research laboratories and acquire more equipment and facilities.

치료경험이 있는 A형 혈우병 환자에서 그린모노^�의 약동학 및 안전성 : 전향적 다기관 공동 임상시험

윤휘중,이순용,황태주,손영택 德成女子大學校 藥學硏究所 2001 藥學論文誌 Vol.12 No.1

배 경 : 최근 국내에서 사용 가능해 진 단클론항체를 이용한 고순도의 제 8응고인자 그린모노^R에 대하여, 제품의 약동학적 측면을 관찰하고, 급성 이상반응 발생 측면의 안전성을 관찰하고자 하였다. 방 법 : 과거 응고인자 치료경험이 있는 제 8응고인자치 5% 미만의 A 형 혈우병 환자를 대상으로, 그린모노를 체중 1kg당 50units 정맥주사한 후 약물역동학적 분석을 시행하였다. 약물 투여 후 48시간까지 이상반응을 관찰하고, 약물투여 전 및 48시간 까지 이상반응을 관찰하고, 약물투여 전 및 48시간 후에 일반혈액 검사, 혈액생화학검사, 요검사 등을 측정하여 비교하였다. 제8응고인자 억제인자를 Bethesda assay를 이용하여 투여 전 및 투여 3~7일 후 검사하였다. 결 과 : 15명의 환자중 연구를 완료한 13명의 자료를 분석하였다. Recovery rate는 99±22%(범위, 71~ 136%) 였으며, 2-compartment model을 이용한 beta phase의 반감기는 15.7±6.6시간(범위, 9.7~35.9시간)이었다. 그린모노^ R 투여후 의미있는 이상반응은 없었으며, 검사성적의 의미있는 변화도 발견할 수 없었다. 제8응고인자 억제인자는 시험약 투여 전후 모두 0.6 BU 미만으로 유지되었다. 결 론: 그린모노^R는 약동학적으로 유효하고, 급성이상 반응이 없어, 임상 이용에유용하리라 생각한다.