Enhanced antitumor immunotherapeutic effect of B-cell-based vaccine transduced with modified adenoviral vector containing type 35 fiber structures

Kim, E-K,Seo, H-S,Chae, M-J,Jeon, I-S,Song, B-Y,Park, Y-J,Ahn, H M,Yun, C-O,Kang, C-Y Macmillan Publishers Limited 2014 Gene therapy Vol.21 No.1

For successful clinical tumor immunotherapy outcomes, strong immune responses against tumor antigens must be generated. Cell-based vaccines compromise one strategy with which to induce appropriate strong immune responses. Previously, we established a natural killer T-cell (NKT) ligand-loaded, adenoviral vector-transduced B-cell-based anticancer cellular vaccine. To enhance tumor antigen delivery to B cells, we established a modified adenoviral vector (Ad-k35) that encoded a truncated form of the breast cancer antigen Her2/neu (Ad-k35HM) in which fiber structure was substituted with adenovirus serotype 35. We observed increased tumor antigen expression with Ad-k35HM in both human and murine B cells. In addition, an Ad-k35HM-transduced B-cell vaccine elicited strong antigen-specific cellular and humoral immune responses that were further enhanced with the additional loading of soluble NKT ligand KBC009. An Ad-k35HM-transduced, KBC009-loaded B-cell vaccine efficiently suppressed the in vivo growth of established tumors in a mouse model. Moreover, the vaccine elicited human leukocyte antigen (HLA)-A2 epitope-specific cytotoxic T-cell responses in B6.Cg (CB)-Tg (HLA-A/H2-D) 2Enge/Jat mice. These findings indicated that the Ad-k35 could be appropriate for the preclinical and clinical development of B-cell-based anticancer immunotherapies.

Potassium-based dry sorbents for removal of sulfur dioxide at low temperatures

Chae, H.J.,Lee, S.C.,Lee, S.J.,Cho, M.S.,Jung, S.Y.,Ryu, C.Y.,Kwon, Y.M.,Hwang, B.W.,Lee, J.B.,Kim, J.C. Korean Society of Industrial and Engineering Chemi 2016 Journal of industrial and engineering chemistry Vol.36 No.-

<P>The SO2 sorption properties of dry potassium -based sorbents, prepared by impregnation of various supports with potassium carbonate, were investigated at the various water vapor concentrations at a level of 5000 ppm of SO2. It was found that alkali metal -based sorbents promoted with potassium carbonate could be used as a sorbent for SO2 sorption at low temperatures (between 30 degrees C and 60 degrees C). In particular, the potassium-based sorbents showed an excellent SO2 sorption capacity of about 310 mg SO2/g sorbent in the presence of water vapor, while in the absence of water vapor, their SO2 sorption capacities decreased. (C) 2016 The Korean Society of Industrial and Engineering Chemistry. Published by Elsevier B.V. All rights reserved.</P>

돈육의 사후 24시간 pH 수준에 따른 육질 특성

박범영,조수현,유영모,김진형,채현석,안종남,김용곤,이종문,윤상기 한국동물자원과학회 2002 한국축산학회지 Vol.44 No.2

도축 후 돈육의 육질평가에 있어 중요한 요인인 사후 24시간 pH를 측정하여 pH가 5.31∼5.50, 5.51∼5.70, ≥5.71에 따른 육질을 조사한 결과, 사후 pH가 더 낮을수록 단백질 함량이 높고, 지방 함량이 낮았다(P<0.05). 사후 24시간 pH가 높을수록 보수력은 증가하였으며, 가열감량과 육즙감량은 유의적으로(P<0.05) 감소하였다. 육의 경도, 탄력성, 씹힘성과 지방경도 역시 사후 24시간 pH가 높을수록 유의적인 증가를 보였다(P<0.05). 육색특성 중 CIE L*값은 사후 24시간 pH가 낮을수록 유의적으로 높았으며, a*와 b*값, chroma 값은 최종 pH가 5.71이상의 돈육에서 유의적으로 낮게 나타났다(P<0.05). Hue 값은 pH 5.31∼5.70 범위에서 가장 높았으며, ΔE 값도 pH 5.31∼5.50에서 가장 높았다. 관능적 특성의 분석결과, 다즙성과 연도는 최종 pH가 높을수록 향상되었으나, 향미는 최종 pH 범위에 따른 차이가 인정되지 않았다. 따라서 본 연구결과, 도축 후 24시간 돈육의 pH 범위에 따라 육질의 차이를 보이기 때문에 사후 적정 pH를 유지할 수 있도록 도축전 관리, 도축방법, 도축 후 냉각 온도관리 등을 개선하는 것이 고품질 돈육생산에 있어 중요한 것으로 사료된다. Meat quality of the domestic pork loins(n=537) classified by 3 groups(5.31∼5.50, 5.51∼5.70 and ≥5.71) according to pH at 24hr post-mortem(pH_24) was investigated. In proximate chemical compositions, protein was highest and fat was lowest in the pork loins of pH_24 5.31∼5.50 group. Water holding capacity increased as pH_24 increased, whereas purge loss and cooking loss decreased as pH_24 increased. Meat color values(CIE L*, a*, b*, Chroma, Hue and ΔE) decreased as pH_24 increased. In texture traits, hardness and chewiness were lowest and fat hardness was highest in the pork loins of pH_24≥5.71 group when compared to the other pH_24 groups. However, Warner-Bratzler Shear force, springiness and cohesiveness were not significantly different among the pH_24 groups(P>0.05). In sensory properties, juiciness and tenderness were highest in pH_24≥5.71 group. From the results of this study, pork quality was highly related to pH_24. Therefore, the factors affecting the post-mortem pH, such as stress before slaughter, slaughtering methods, and cooling condition slaughter must be properly controlled and improved to produce high quality pork.

EFFECTS OF VITAMIN E AND SELENIUM SUPPLEMENTATION TO DIETS CONTAINING AFLATOXIN B₁ ON THE CONTENTS OF LIVER LIPIDS AND VARIOUS BLOOD PARAMETERS IN RATS

Choi, Y.K.,Jung, K.K.,Chae, K.Y.,Jang, I.,Lee, B.D.,Nahm, K.H. Asian Australasian Association of Animal Productio 1995 Animal Bioscience Vol.8 No.4

Ninety Wistar male rats were used to study the effects of vitamin E and Se supplementation to diets containing aflatoxin $B_1$ on the contents of liver lipids and various blood parameters. Two levels of dietary aflatoxin (0 and 1 ppm), 3 levels of vitamin E (30, 60 and 120 IU/kg), and 3 levels of Se (0.1, 1 and 2 ppm) were used to design a $2{\times}3{\times}3$ factorial experiment. Rats, weighing about 200 g, were randomly allotted to 18 cages, 5 rats per cage. The aflatoxin significantly (p < .05) decreased growth rate, feed intake and feed efficiency. Aflatoxin increased the glucose level and decreased the cholesterol level in blood significantly. Levels of blood triglyceride, total protein, and albumin were not affected by aflatoxin, vitamin E or Se. Activities of blood alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were significantly increased by aflatoxin; however, the glutathione peroxidase (GSH-Px) activity in the blood was decreased by aflatoxin even in the presence of Se. The vitamin E supplementation decreased the AST activity significantly, while GSH-Px activity increased significantly as the levels of dietary Se increased. The levels of total cholesterol and free cholesterol in the liver were significantly lower in rats receiving aflatoxin, while the extra vitamin E supplementation increased these hepatic cholesterol levels. It was concluded that the extra dietary vitamin E or Se supplementation might partially alleviate some of the harmful effects of aflatoxin in rats.

비선형 역기전력 파형을 고려한 BLDC 모터 모델

이상용(S.Y. Lee),강병희(B.H. Kang),채영민(Y.M. Chae),목형수(H.S. Mok),초규하(G.H. Choe),김덕근(D.K. Kim),류재성(J.S. Ryu) 전력전자학회 1999 전력전자학술대회 논문집 Vol.1999 No.7

A brushless DC motor has the high quality of torque output and silence, has been more widely used in industrial area. As the driver and controller of BLDC motor have been more complicated and precise, simulation method has been much used m motor design. And the output characteristics of BLDC motor is determined by the waveform of BACK-EMF in instinct. But because the conventional model of BLDC motor is obtained by approximation of real nonlinear waveform to ideal trapezoidal waveform, the error is occurred in slmulation result. Thus in this paper, for the correction of this error in simulation, the model of real nonlinear waveform considered is proposed, and the simulation result is obtained in case of three-phase, four-poles Y-connected, surface mounted permanent magnet BLDC motor.

4-Phenylbutyric acid regulates CCl4-induced acute hepatic dyslipidemia in a mouse model: A mechanism-based PK/PD study

Lee, H.Y.,Marahatta, A.,Bhandary, B.,Kim, H.R.,Chae, H.J. North-Holland ; Elsevier Science Ltd 2016 european journal of pharmacology Vol.777 No.-

<P>Endoplasmic reticulum (ER) stress and associated protein aggregation are closely associated with human diseases, including alterations in hepatic lipid metabolism. Inhibition of ER stress can have a significant effect on the prevention of hepatic dyslipidemia. Here, we studied the role of 4-phenylbutyric acid (4-PBA), a chemical chaperone, on ER stress-induced hepatic lipid accumulation. We studied ER stress induction following CCl4 exposure and delineated mechanisms of the CCl4-induced ER stress response in liver tissue from mice. CCl4 affected the formation of disulfide bonds through excessive hyper-oxidation of protein disulfide isomerase (PDI). Increased complex formation between PDI and its client proteins persisted in CCl4-exposed samples. Conversely, 4-PBA inhibited ER stress via secretion of apolipoprotein B and prevention of hepatic lipid accumulation. We also studied the mechanism-based pharmacokinetic and pharmacodynamic profiles and identified the ER stress-related proteins GRP78 and CHOP, along with plasma apolipoprotein B and triglyceride levels, as novel biomarkers of ER stress-induced hepatic lipid accumulation. ER stress and its clinical relevance for therapeutic approaches were well correlated with the activity of the ER stress regulator 4-PBA, which may be a promising drug candidate for the treatment of hepatic lipid accumulation, such as hepatic steatosis. (C) 2016 The Authors. Published by Elsevier B.V.</P>

Application of genetically engineered Salmonella typhimurium for interferon-gamma-induced therapy against melanoma

Yoon, W.,Park, Y.C.,Kim, J.,Chae, Y.S.,Byeon, J.H.,Min, S.H.,Park, S.,Yoo, Y.,Park, Y.K.,Kim, B.M. Pergamon Press 2017 European journal of cancer Vol.70 No.-

Salmonella have been experimentally used as anti-cancer agents, because they show selective growth in tumours. In this study, we genetically modified attenuated Salmonella typhimurium to express and secrete interferon-gamma (IFN-γ) as a tumouricidal agent to enhance the therapeutic efficacy of Salmonella. IFN-γ was fused to the N-terminal region (residues 1-160) of SipB (SipB160) for secretion from bacterial cells. Attenuated S. typhimurium expressing recombinant IFN-γ (S. typhimurium (IFN-γ)) invaded the melanoma cells and induced cytotoxicity. Subcutaneous administration of S. typhimurium (IFN-γ) also efficiently inhibited tumour growth and prolonged the survival of C57BL/6 mice bearing B16F10 melanoma compared with administration of phosphate-buffered saline (PBS), unmodified S. typhimurium or S. typhimurium expressing empty vector (S. typhimurium [Vec]) in a natural killer (NK) cell-dependent manner. Moreover, genetically modified Salmonella, including S. typhimurium (IFN-γ), showed little toxicity to normal tissues with no observable adverse effects. However, S. typhimurium (IFN-γ)-mediated tumour suppression was attributed to direct killing of tumour cells rather than to stable anti-tumour immunity. Collectively, these results suggest that tumour-targeted therapy using S. typhimurium (IFN-γ) has potential for melanoma treatment.

국내 분리 일본뇌염 바이러스 유전자에 대한 항체생성 연구(Ⅰ) : cloning of JEV viral genes

박찬,최우영,이호동,채수림,신은진,김인범,표재환,박근용,조해월 국립보건연구원 1999 국립보건원보 Vol.36 No.-

살충제에 대한 중요 모기종의 감수성 조사연구

심재철,윤영희,조양벽,이주연,신이현,양영철,이욱교,박정은,채숙희 국립보건연구원 1994 국립보건원보 Vol.31 No.1

WITHDRAWN: Evaluating the association between body weight and the intestinal microbiota of weaned piglets via 16S rRNA sequencing

Han, G.G.,Lee, J.Y.,Jin, G.D.,Park, J.,Choi, Y.H.,Chae, B.J.,Kim, E.B.,Choi, Y.J. Elsevier Scientific Pub. Co 2016 Veterinary microbiology Vol.196 No.-

<P>Due to the ban on the use of antimicrobial growth promoters in livestock feeds, understanding the relationship between intestinal microbiota and the physiology of the host has become very important for improving livestock performance. In this study, we investigated the relationship between intestinal microbiota and body weights of weaned piglets. Lighter (n=9) and heavier (n=9) 9-week-old weaned piglets were selected from approximately one-hundred individuals based on their body weights. Their fecal microbial communities were analyzed by sequencing the V4 region of the 16S rRNA gene. The microbial richness estimators of the heavier piglets, were significantly higher than those of the lighter piglets. At the phylum level, the microbiota of the heavier group had significantly higher levels of Firmicutes and a higher Firmicutes-to-Bacteroidetes ratio than that of the lighter group. At the genus level, the levels of several genera, such as Anaerococcus and Lactococcus, were significantly different in the two groups. In particular, the lighter group had significantly higher levels of opportunistic pathogenic bacteria, such as Anaerotruncus and Bacteroides, compared with those of the heavier group. Moreover, the levels of bacteria expressing the components of several metabolic pathways were significantly different in the two groups. The microbiota of the heavier group had a significantly higher involvement in three KEGG pathways concerned with xenobiotic degradation than that of the lighter group. These results may provide insights into host-microbe interactions occurring in the piglet intestine and will be useful in establishing a strategy for improving growth performance in the swine industry. (C) 2016 Elsevier B.V. All rights reserved.</P>