국내 검정콩 육성품종의 종자특성

주용하,박재훈,윤승길,김영호,김성민,정길웅 한국국제농업개발학회 2002 韓國國際農業開發學會誌 Vol.14 No.4

1. 수분흡수율이 가장 높은 시간은 다원콩(침지 후 4시간)을 제외한 모든 품종이 침지후 2시간이었으며, 둔화되기 시작한 시간은 침지후 약 10시간이었고, 평형에 도달한 시간은 침지후 약 16시간이었다. 2. 수분흡수율은 품종간에 차이가 있었는데 가장 높은 수분흡수율을 보인 품종은 검정콩2호이었으며, 가장 낮은 품종은 다원콩이었다. 3. 발아율의 범위는 28이었으며, 평균발아율은 93.9%이었고, 가장 높은 품종은 청자콩과 흑청콩이었다. 4. 발아세의 범위는 52이었으며, 평균발아세는 81.9%이었고, 가장 높은 품종은 흑청콩이었다. 5. 평균발아일수와 T_50의 범위는 모두 2이었으며, 평균은 각각 2.6과 2.3이었고, 품종간에는 선흑콩이 가장 길었다. 6. 발아균일도의 범위는 3.1이었으며, 평균은 1.3이었고, 높은 그룹(흑청콩·청자콩·검정콩2호·다원콩·검정올콩)과 낮은 그룹(검정콩1호·일품검정콩·선흑콩)으로 분류되었다. 7. 알칼리붕괴도는 공시품종의 평균이 4.65이었으며 등급은 4∼5등급을 나타내었다. 붕괴도가 가장 높고 우수한 품종은 검정콩1호였으며 가장 낮은 품종은 선흑콩이었다. The water absorption rate after soaking of seeds was the highest at 2 hours in all varieties except Dawonkong(4 hours after soaking), and became slowed down at 10 hours, and reached moisture equilibrium in stopping almost at 16 hours. The water absorption rate was different among varieties, the highest variety was Geomjeongkong #2 whereas the lowest variety was Dawonkong. The range of germination percentage was 28, and average value 93.9%, and the highest varieties were Cheongjakong and Heukcheongkong. The range of germination speed was 52. average value 81.9%, and the highest variety was Heukcheongkong. The range of average days to germination and T_50 were all two days and these average was 2.6 and 2.3, respectively. Seonheukkong among varieties was very long in average days to germination and T_50. The range of germination uniformity was 3.1 and average was 1.3 and divide into two groups such as high group(Heukcheongkong, Cheongjakong, Gemjeongkong #2, Dawonkong, Geomjeongolkong) and low group(Geomjeongkong #1, llpumgeomjeongkong, Seonheukkong). Varietal mean of alkali digestibility value was 4.65 belong to 4∼5 class, the highest variety was Geomjeongkong #1 whereas Seonheukkong was very low among varieties.

백서에서 N-dimethylnitrosamine에 의한 간독성 작용에 L-ascorbic Acid가 미치는 영향에 관한 연구

송준민,양승하,변광의,강상균 순천향대학교 1994 논문집 Vol.17 No.2

Toxic effect of NDMA on liver is variable due to dose and duration of administration. Experimental studies showed hemorrhagic necrosis, liver cirrhosis and hepatoma development with NDMA administration. To observe the protective action of L-ascorbic acid on NDMA toxicity of the liver, experimental studies were carried out. Rats were divided into two groups' acute and chronic intoxication. Acute group was divided into three subgroups regarding to dose and duration of NDMA administration, AGI-1(NDMA 40㎎/㎏) and AGI-2(NDMA with L-ascorbic acid 720㎎/㎏), AGII-1(NDMA 20㎎/㎏ for 2 days) and AGII-2(same with AGII-1 with AGII-1 with L-ascorbic acid 360㎎/㎏ for first day, and 10㎎/㎏, 180㎎/㎏ for 2nd and third day). Serological study of liver function and histologic study were carried out 48 hours after NDMA administration. Animal were sacrified on 9th week, then serological and histological studies were carried out. Also ultrastructural observation were performed for the liver tissue. The results were following as : 1. Acute NDMA intoxication group reveals low level of serum protein and albumin while elevation of total bilirubin, alkaline phosphatase and marked elevation of SGOT and SGPT. Histologically, marked hemorrhagic necrosis was noted in the central zone of hepatic lobules. 2. In chronic NDMA intoxication, serological studies showed slight elevation of SGOT and SGPT, and normal levels of total protein, total bilirubin, and ALP. Histologically, swelling of hepatocyte, necrosis of individual cells, enlargement of nucleus with prominent nucleoli were evident. 3. Chronic intoxication of NDMA groups does not showed special difference between NDMA alone and combined L-ascorbic acid administration. 4. Both acute and chronic NDMA intoxication groups showed ultrastructural changes, such as nuclear irregularity, dilatation of cisternae of RER, partially obscured cisternae with increased density of mitochondira, and focal lipid droplet accumulation in the cytoplasm. The above results show that L-ascorbic acid may play a protective role in toxicity of NDMA.

최소 절개를 통한 경미한 구순열의 교정

박승하,윤을식,구상환,민병두,김우경 大韓成形外科學會 1999 Archives of Plastic Surgery Vol.26 No.5

Microform cleft lip is a mild form of incomplete cleft lip, also known as a minimal occult, abortive, forme fruste cleft lip. However, it has no definition and few methods have been reported for its correction. A microform cleft lip is characterized as the incomplete union of the superficial portion of the orbicularis oris muscle. It is more prominent during facial expression than in a resting state. We confined microform cleft lip in our study to the absence of philtral skin change and a contracted position on the top of cupid's bow. During the past 5 years, 17 patients of microform cleft lip were operated on. We corrected the defect of the upper vermilion border and nostril sill with minimal incision, and repaired the underlying lip musculature in superficial discontinuity. Reduction of the widened alar base was performed. Deformed alar cartilage was dissected via rim incision, and suspended in a medial and upper direction with pull-out sutures. The most important thing is precise repair of the superficial portion of the separated orbicularis oris muscle via minimal incision, and it is best to operate after 1-year of age for accurate repair. The results were satisfactory and the parents were also satisfied. The advantages of this procedure are as follows: 1. Less visible, minimal scar on upper lip 2. Simultaneous correction of vermillion notching, deformed cupid's bow and nasal deformity. 3. Eversion of philtral ridge due to tenting effect of horizontal mattress suture 4. Philtral elongation effect by reduction of alar base and Z-plasty of cupid's bow.

자가 골수 중간엽줄기세포와 생분해성 고분자 스캐폴드를이용한 조직공학적 인공혈관 제조

조승우,김동익,박희정,임상현,전오주,김수현,김영하,최차용,이민재,김종성,장인성,김병수 대한혈관외과학회 2003 Vascular Specialist International Vol.19 No.2

Ethylenediaminetetraacetic acid (EDTA)항응고제에 의한 Mean Corpusculaar Volume(MCV)변화

홍승민,박영진,최하여,임진,정옥연,이호준,이호영,장숙진,문대수 조선의대 1996 The Medical Journal of Chosun University Vol.21 No.2

Epstein-Barr 바이러스 형질전환법을 이용한 파상풍 톡소이드에 대한 사람 단세포군 항체의 생산

유승민,조정제,호순태,하윤문 大韓免疫學會 1993 大韓免疫學會誌 Vol.15 No.-

For production of tetanus toxoid(TT) specific human monoclonal antibodies, anti-TT antibody secreting peripheral B lymphocytes were separated by rosetting with TT-coated SRBC. And, B lymphoblastoid cell line(BLCL) was established by Epstein-Barr Virus(EBV) transformation methods. Stable BLCLs were established after three times of cloning by limiting dilution. To establish the maximal antibody producing condition, several environmental factors were tested. The optimal condition for maximum antibody prodiction was 4 day culture of 1 x 105cells/ml concentration in RPMI 1640 complete culture media supplemented with 20% fetal calf serum, and the maximum concentration of secreted antibody was about 900ng/ml. The antibody production of BLCL was decreased during long-term culture after establishment of cell line, but antibody production was maintained by repeated cloning by limiting dilution.

Ethylenediaminetetraacetic acid(EDTA)항응고제에 의한 Mean Corpuscular Volume(MCV)변화

홍승민,박영진,최하영,임진,정옥연,이호준,이호영,장숙진,문대수 朝鮮大學校 附設 醫學硏究所 1996 The Medical Journal of Chosun University Vol.21 No.2

It is essential that we put the anticoagulant into the blood specimen of the CBC test. However, the value of some parameters in the CBC test can be changed whenever using some anticoagulants. EDTA anticoagulant is mostly used these days. There are 3 kinds of EDTA, that is K_(2-), K_(3-), and Na_(2-) EDTA. The author studies how many effects each the EDTA gets into the true value of blood specimen for MCV of RBCs. Especially we compared the MCV effect of different concentrations of 3 EDTA anticoagulants on the bias with time and instrument. Some results are gotten. In our results, K_(3-)EDTA anticoagulant shows more satisfactory MCV value than K_(2) or Na_(2-)EDTA. On using concentration of EDTA, 1.5㎎ EDTA/㎖ of blood show more satisfactory MCV value than 7.5㎎ EDTA/㎖ of blood. However, the choice of anticoagulant may be dependant on the different instruments.

랫드에서 l-muscone의 급성독성 및 아급성독성시험 연구

오승민,연제덕,남혜운,박대규,조명행,정규혁 성균관대학교 약학연구소 1998 成均藥硏論文集 Vol.10 No.1

l-Muscone is synthesized for use as substitutive material of musk which is the active ingredient of woohwangchungsimwon. The objective of this investigation was to evaluate the acute and subacute toxicity of l-muscone in rats. In oral acute toxicity test, SPF Sprague-Dawley male and female rats were gavaged with l-muscone of two doses(0, 5.0g/㎏). No dead animal and abnormal autopsy findings were found in control and treated group. Body weights were slightly decreased in both sexes of rats treated with 5.0g/㎏. Therefore, oral LD_50 of l-muscone was consider to be higher than 5.0g/㎏ in male and female rats. In intraperitoneal acute toxicity test, rats were injected intraperitoneally with dosages of 0, 1,000, 1,316, 1,732, 2,279 and 3,000 ㎎/㎏. Decreased body weights and motor activities were observed at high dose group. Intraperitoneal LD_50 of l-muscone were 1,920 ㎎/㎏ in male and female rats. In the subacute study. l-muscone was administrated orally to both sexes of rats for 4 weeks as several doses(0, 10, 100 and 1,000 ㎎/㎏). There were neither dead animals nor significant changes of body weights during the experimental period. In addition, no differences were found between control and treated groups in clinical signs, urinalysis, hematology, serum biochemical analysis, and other findings. Above data suggest that no observed adverse effect level of l-muscone in rats might be over 1,000 ㎎/㎏/day in this study.

각종 피부종양에서 p53과 bcl-2 발현양상

정용민,양승하,이성열,김대중,김의한 순천향의학연구소 1999 Journal of Soonchunhyang Medical Science Vol.5 No.1

Background : The function of p53 protein was known to accelerate the apoptosis, but bcl-2 protein was known to suppress the apoptosis. The overexpression of p53 and bcl-2 proteins has been reported in many malignant tumors. Although the mechanism of tumorgenesis of the p53 and bcl-2 protein took hasnot been proved, bcl-2 positive tumors contrast to p53, appeared to have good prognosis. The expression pattern of p53 and bcl-2 in epidermal tumors has not been well known. The author analyzed immunohistochemical expression of p53 and bcl-2 protein and investigated the role of p53 and bcl-2 protein in the developing tumors of epidermal origin. Method : 15 cases of squamous cell carcinoma, 20 cases of basal cell carcinoma, 10 cases of keratoacanthoma and 6 cases of Bowen's disease were stained immunohistochemically stain with anti p53 and anti bcl-2 monoclonal antibody. Results : 1. In squamous cell carcinoma, the reaction of p53 was positive in 13 cases(86%) and the stain pattern showed more intensive reaction in poorly differentiated cells in the same tumor. bcl-2 showed negative in all cases of squamous cell carcinoma(0%). 2. In basal cell carcinoma, the reaction of p53 was positive in 10 cases (50%). The positive cells were mainly observed in squamous metaplastic portion, but the reaction was weak. bcl-2 showed positive in all cases of basal cell carcinoma (100%) and the reaction was strong. 3. In keratoacanthoma, p53 was positive in 7 cases (70%). The positive cells were mainly observed in the cells around the basement membrane. This finding suggested that p53 positive basal cells were programmed to become keratoacanthoma. bcl-2 was negative in all 10 cases of keratoacanthoma. (0%) In some cases, bcl-2 positive cells were observed around the basement membrane in some cases., which were negative to p53 This finding suggested that the existence of these bcl-2 positive cells were related to the prognosis of keratoacanthoma. 4. In Bowen's disease, p53 was positive in 5 cases ( 83% ) and the reaction was strong, so the tumor cells in Bowen's disease appeared to be poorly differentiated type. bcl-2 was negative in all 6 cases ( 0% ) of Bowen's disease, but bcl-2 positive cells were observed in the basement membrane in some cases.

TC볼트의 스트리핑 메카니즘에 대한 실험적 연구

손승요,신근하,홍민성 韓國工作機械學會 2000 한국생산제조학회지 Vol.9 No.1

Over tightening experiments of the tension-controlled bolts are caned out and the stripping mechanism is reviewed based on the observed results. There arc two modes of bolt failure due to the over tightening : one the fracture of the bolt, the other the thread stripping. Bifurcation between these two modes is rather delicate, but it seems being related with the elastic flexibility of the bolt, which depends upon the unused thread length. The fracture mode occurs in the bolts with good flexibility, while the latter with bad one. According to the ISO Standard, some meter coarse threads like M20 and M22 have the same pitch, which causes bigger fastener to less resistance in shear and bending compared with the smaller one. However, since UNC thread system adapts different pitch for different nominal diameter, unified coarse threads show better stripping resistance than their corresponding meter threads.