Dimethylnitrosamine 유발 급성 간 손상 흰쥐에서 ^(99m)Tc-Lactosylated Serum Albumin을 이용한 간 기능의 평가

정신영,이재태,서명랑,유정아,배진호,안병철,황재석,정재민,하정희,이규보 대한핵의학회 2003 핵의학 분자영상 Vol.37 No.6

목적: ^(99m)Tc-lactosylated serum albumin (^(99m)Tc-LSA)은 간세포에 특이적으로 결합하는 간수용체 영상용 방사성의약품으로 새로이 합성되었다. 간섬유화를 유발하는 dimethylnitrosamine (DMN)을 투여한 간 손상 휜쥐 모델에서 ^(99m)Tc-LSa의 역동학적인 간섭취를 조사하고 간효소치의 변화와 조직학적 소견을 비교하여, LSA의 간섭취가 간기능의 변화를 반영하는지를 연구하였다. 대상 및 방법: SD계 흰쥐에 DMN를 27 mg/kg으로 복강 내 주사하여 급성 간손상을 유도하고 대조군과 비교하였다. DMN을 주사한 흰쥐를 3일(DMN-3), 8일(DMN-8), 21일(DMN-21)에 ^(99m)Tc=LSA (1,665 mg/kg) 29 MBq를 정맥 주사하여, 30분 동안 동적 영상을 획득하고 간과 신장부위에 관심영역을 설정하여 간과 심장부위의 시간방사능 곡선을 얻었다. 간기능 평가를 위해 시간방사능 곡선을 이용하여 간섭취지수와 혈중제거지수를 구하였고 곡선 최적화를 시행하였다. DMN 투여군과 대조군의 간효소치의 변화와 간조직의 광학현미경 소견을 비교하였다. 결과: 대조군에서는 ^(99m)Tc-LSA가 빠르게 간에 섭취되고 혈중에서 제거되었으나 DMN을 처리한 군에서는 간섭취가 낮았다. 간섭취지수의 비교에서 대조군에 비해 DMN 처리군에서 유의하게 간섭취지수가 낮았다(DMN-3: 0.842, DMN-8: 0.898, DMN-21: 0.91, 대조군: 0.96, p<0.05). 혈중제거지수의 비교에서도 대조군에 비해 DMN 처리군에서 혈중제거지수가 유의하게 높았다(DMN-3: 0.731, DMN-8: 0.654, DMN-21: 0.604, 대조군: 0.473, p<0.05). 비선형 회귀분석에서 R_(2) 값은 0.9이상으로 좋은 일치를 보였고, 대조군에ㅓ K값이 DMN처리군에 비해 크고(DMN-3: 0.28, DMN-8: 0.41, DMN-21: 0.46, 대조군: 0.97, p<0.05), T_(1/2)값은 작았다(DMN-3: 2.5, DMN-8: 1.7, DMN-21: 1.5, 대조군: 0.7, p<0.05). 간효소치의 변화는 DMN-3군에서는 대조군에 비해 상승하였으나 DMN-8, DMN-21군에서는 간효소치의 상승이 관찰되지 않았다. 간조직 소견의 경우 DMN-3군에서 중심정맥 주위에 괴사가 관찰되었으나 DMN-8군, DMN-21군에서는 미약한 정도의 염증세포 침윤만이 관찰되었다. 결론: ^(99m)Tc-LSA 간신티그래피의 간섭취 정도는 간손상과 반비례하였으며 간섭취의 변화는 조직학적 손상이 심한 정도와 간손상후 회복되는 과정을 반영하여 주었다. ^(99m)Tc-LSA 간신티그래피가 간손상을 평가하고 간손상후 회복되는 과정을 추적하는 간수용체 영상용 방사성 의약품으로 사용될 수 있을 것으로 생각된다. Objects: ^(99m)Tc-lactosylated human serum albumin(LSA) is a newly synthesized radiopharmaceutical that binds to asialoglycoprotein receptors, which are specifically presented on the hepatocyte membrane. Hepatic uptake and blood clearance of LSA were evaluated in rat with acute hepatic injury induced by dimethylnitrosamine(DMN) and results were compared with corresponding findings of liver enzyme profile and these of histologic changes. Materials and Methods: DMN (27 mg/kg) was injected intraperitoneally in Sprague-Dawley rat to induce acute hepatic injury. At 3(DMN-3), 8(DMN-8), and 21(DMN-21) days after injection of DMN, LSA injected intravenously, and dynamic images of the liver and heart were recorded for 30 minutes. Time-activity curves of the heart and liver were generated from regions of interest drawn over liver and heart area. Degree of hepatic uptake and blood clearance of LSA were evaluated with visual interpretation and semiquantitative analysis using parameters (receptor index : LHL3 and index of blood clearance : HH3), analysis of time-activity curve was also performed with curve fitting using Prism program. Results: Visual assessment of LSA images revealed decreased hepatic uptake in DMN treated rat, compared to control group. In semiquantitative analysis, LHL3 was significantly lower in DMN treated rat group than control rat group (DMN-3:0.842, DMN-8: 0.898, DMN-21: 0.91, Control: 0.96, p<0.05), whereas HH3 was significantly higher than control rat group (DMN-3: 0.731, DMN-8: 0.654, DMN-21: 0.604, Control: 0.473, p<0.05). AST and ALT were significantly higher in DMN-3 group than those of control group. Centrilobular necrosis and infiltration of inflammatory cells were most prominent in DMN-3 group, and were decreased over time. Conclusion: The degree of hepatic uptake of LSA was inversely correlated with liver transaminase and degree of histologic liver injury in rat with acute hepatic injury.

ORiginal Article : Gastroprotective Effects of PMK-S005 against Ethanol-Induced Acute Gastric Damage in Rats

( Yoon Jeong Choi ),( Nayoung Kim ),( Ju Yup Lee ),( Ryoung Hee Nam ),( Ji Hyung Seo ),( Seonmin Lee ),( Hee Jin Kim ),( Yoon Jin Choi ),( Hye Seung Lee ),( Dong Ho Lee ) 대한간학회 2016 Gut and Liver Vol.10 No.3

Background/Aims: This study aimed to examine the gastroprotective effects of PMK-S005, which is a synthetic S-allyl-Lcysteine (SAC; a sulfur-containing amino acid), against acute ethanol-induced gastric damage in rats. Methods: Sprague- Dawley rats were divided into six groups, including a nonethanol group, groups treated with absolute ethanol 1 hour after pretreatment with various doses of PMK-S005 (1, 5, and 10 mg/kg) or rebamipide (50 mg/kg), and an absolute ethanolonly group. Ethanol-induced gross ulcer and mucus levels were measured. Myeloperoxidase, tumor necrosis factor a, interleukin 1β, PGE2, LTB4, cPLA2, COX-1, and COX-2 levels were estimated by enzyme-linked immunosorbent assay or Western blot analysis. Furthermore, the protein expression levels of antioxidant enzymes, including heme oxygenase-1 (HO-1), NAD(P)H:quinine oxidoreductase 1 (NQO-1), GCLC, and GCLM, were assessed. Results: PMK-S005 significantly attenuated the ethanol-induced gastric damage; it reduced mucosal inflammatory cytokine production and increased mucus levels. The expression levels of cPLA2, COX-1, and COX-2 were decreased by PMK-S005. PMK-S005 did not affect PGE2 synthesis, but LTB4 production was significantly suppressed. In addition, long-term administration of PMKS005 significantly increased the expression of HO-1, NQO-1, GCLC, and GCLM. Conclusions: These results strongly suggest that PMK-S005 prevents gastric mucosal damage and that these gastroprotective activities are due to anti-inflammatory effects and enhancement of the gastric defense system, including antioxidant enzymes. (Gut Liver 2016;10:348- 355)

노안당 편액의 제작기법과 효율적인 보존방안

양필승,서정훈,서정호,Yang, Pil-Seung,Seo, Jeong-Hun,Seo, Jeong-Ho 국립문화재연구소 2008 保存科學硏究 Vol.29 No.-

'No-An-Dang' in 'Un-Hyeun palace' was using as a guesthouse by 'dea-won-goon' built in 1864. 'No-An-Dang tablet' exhibited in 'No-An-Dang' seems to be made by using the letters which were written by 'Choo-sa Kim Jeong-Hee'. This tablet was manufactured by special processes; wood frame which was made of a Korean white pine was drawn patterns by variety pigment and latticed inside are joined with 3 of horizontal square wooden sticks and 7 of vertical square wooden sticks, then several sheets of the paper mulberry put on this frame and sheets cut along the edge of letter attached lastly. This structure of tablet could cause more conservation problems than only made in wooden structures. Therefore, replica was made and exhibited in bad condition and original one kept in museum storage for effective conservation of artefacts.

키넥트 센서를 이용한 고령자 대상의 선자세 균형능력 평가

양승태(Seung-Tae Yang),강동원(Dong-Won Kang),서정우(Jeong-Woo Seo),김대혁(Dae-Hyeok Kim),김태호(Tae-Ho Kim),최진승(Jin-Seung Choi),탁계래(Gye-Rae Tack) 대한전기학회 2017 전기학회논문지 Vol.66 No.2

Portable low-cost Kinect sensor was used to analyze standing balance ability of the elderly. Eighty subjects who can walk alone and have a normal cognitive level participated in this experiment. Based on Berg Balance scale (BBS) test with 52 points, subjects were divided into Healthy older (HO: 46 persons, BBS: 53.80 ± 1.19) and Impaired older (IO: 34 persons, BBS: 49.06 ± 2.03) group. Each subject performed 30 seconds four different standing balance tests (EO: Eyes Open, EC: Eyes Close, EOf: Eyes Open on foam, ECf: Eyes Close on foam). Five variables (Mean distance, Range of distance, Root mean square, Mean velocity, 95% ellipse area) were calculated from the hip joint center movement of Kinect sensor. Results showed that there were significant differences between groups for four different standing tests. Calculated variables from kinect sensor showed significant correlation with BBS score. Especially, mediolateral mean distance, mediolateral root mean square, mediolateral range of distance and 95% ellipse area showed discriminative ability for all tests. Mean values of variables of IO were higher than those of HO, which means the decreased balance ability in IO compared with HO. Therefore, it was possible to estimate simple balance assessment of the elderly using portable low-cost Kinect sensor.

The Antioxidant Effect of the Rhaponticum uniflorum Extracts on the Oxidative Stress in H₂O₂ induced Human Keratinocytes

So Hee Ahn,Won Yeoung Choi,Ji Won Jeong,Geon Woo Park,Su Yeon Kang,Eun Jeong Kim,Su Rim Sin,Eun Seo Jang,Gye Won Lee,Young Ho Cho 한국식품영양과학회 2021 한국식품영양과학회 학술대회발표집 Vol.2021 No.10

In this study, To investigate the antioxidant activities of Rhaponticum uniflorum extracts (RUE), we assessed scavenging activities on DPPH, ABTS radicals, and reducing power. The DPPH and ABTS radical scavenging activities (SC50) of RUE were 96.97±1.19 μg/mL and 31.26±0.13 μ g/mL, respectively, and the reducing power significantly increased dose-dependent manner. To study the antioxidant effecters of RUE in H₂O₂ induced human keratinocytes, expression of Nuclear factor erythroid-2-related factor 2 (Nrf2) and heme oxygenase-1 (HO-1) were determined by Western blot and qRT-PCR. RUE induced the expression of HO-1, a critical target enzyme of Nrf2, in a concentration-dependent manner. The expression of HO- 1 mRNA was also induced in H₂O₂-treated HaCaT cells but no significant difference in the level of HO-1 mRNA was observed. Taken together, these results suggest that RUE may be useful as a potential ingredient for anti-aging products.

SFRC의 휨-압축 거동

鄭瑞鎬,朴弘用,趙勇範 明知大學校 産業技術硏究所 2008 産業技術硏究所論文集 Vol.27 No.-

When steel fiber reinforced concrete is used as an underground cavity or a lining of tunnel, the SFRC is subjected to loads which differ in type from those present in standard testing methods. This study is worthy of notice on this point. This study is aimed to investigate an experimental study on flexural-compression behavior of steel fiber reinforced concrete. When a combined bending moment-normal force loading is characteristic in such shells, it were aimed to construct a data for design under such conditions An experimental variables are steel fiber content, shape ratio, angle of end mill. Total 126 specimens using four different kinds of steel fiber products were evaluated from loading test. From the test results, it was known that a fracture occurred before reaching ε_(cu). A tensile strain was gauged 5‰ at that time. SFRC specimens occurred a tensile fracture, It was known that a location of neutral axis was not changed untill a crack occurred on specimens.

3차원 전산화단층촬영 영상을 이용한 얼굴 연조직 두께 계측

정호걸,김기덕,한승호,신동원,허경석,이제범,박혁,박창서 대한구강악안면방사선학회 2006 Imaging Science in Dentistry Vol.37 No.1

Purpose : To evaluate accuracy and reliability of program to measure facial soft tissue thickness using 3D computed tomographic images by comparing with direct measurement. Materials and Methods : One cadaver was scanned with a Helical CT with 3 mm slice thickness and 3 mm/sec table speed. The acquired data was reconstructed with 1.5 mm reconstruction interval and the images were transferred to a personal computer. The facial soft tissue thickness were measured using a program developed newly in 3D image. For direct measurement, the cadaver was cut with a bone cutter and then a ruler was placed above the cut side, The procedure was followed by taking pictures of the facial soft tissues with a high-resolution digital camera. Then the measurements were done in the photographic images and repeated for ten times. A repeated measure analysis of variance was adopted to compare and analyze the measurements resulting from the two different methods. Comparison according to the areas was analyzed by Mann-Whitney test. Results : There were no statistically significant differences between the direct measurements and those using the 3D images (p>0.05). There were statistical differences in the measurements on 17 points but all the points except 2 points showed a mean difference of 0.5 mm or less. Conclusion : The developed software program to measure the facial soft tissue thickness using 3D images was so accurate that it allows to measure facial soft tissues thickness more easily in forensic science and anthropology. (Korean J Oral Maxillofac Radiol 2006; 36 : 49-54)

인터넷 서비스 모델에 관한 연구

정연서,음호식 공주영상정보대학 1998 논문집 Vol.5 No.-

본 논문에서는 차세대 인터넷망에서 기존의 최선 서비스 외에 실시간 서비스를 제공하기 위한 확장 인터넷 서비스 모델들에 대해서 기술하고, 이를 제공하기 위한 두 가지 모델들에 대해서 조사 분석하였다. 그러나 살펴 본 바와 같이 통합 서비스를 구현하기 위해 필수적인 RSVP를 사용함에 있어 RSVP 자체가 갖는 확장성 문제와 이 매카니즘을 운용하는데 드는 많은 비용 문제는 통합 서비스를 차세대 인터넷망에 적용하기 힘들 것이다. 또한 통합 서비스 모델을 차세대 인터넷망에 적용할 경우 망이 지나치게 복잡해지고 망이 비효율적으로 운용된다. 반면, 비슷한 차세대 인터넷 서비스 모델인 차별화 서비스는 기본적으로 RSVP를 사용하지 않음으로써 통합 서비스에서 RSVP의 사용에 따른 확장성과 비용 문제를 해결했다. 게다가, 망의 복잡성이나 관리 매카니즘에 대해서도 만족할만한 성능을 제공함으로 차세대 인터넷망에 실질적으로 적용될 수 있는 서비스 모델로 고려되고 있다. 차별화 서비스는 기존 망이나 패킷 전송 매카니즘의 대폭적인 교체 없이 차세대 인터넷망에서 사용자의 다양한 서비스 요구와 서비스 품질을 충족시켜 줄 것이다. 현재 RSVP를 광역망 환경이 아닌 지역망에서 운용하고자 하기 위한 변경 작업이 이루어지고 있다. 지역망에서는 RSVP를 이용한 자원예약 방식이 광역망 환경에서는 차별화 서비스를 이용한 방법이 운용되게 될 것이다. 향후 IP 헤더 필드에서의 차별화 정보의 이용과 트래픽 감시자에서의 패킷 처리 매카니즘에 따른 성능 분석과 각 ISP간의 PHB별 코드포인트 변환, 차별화 서비스 제공에 따른 과금 개선 방법 등에 대한 계속적인 연구가 필요할 것이다.

변형된 smooth 모델을 이용한 비선형 이력 거동의 해석적 연구

서민정,문정호,이리형 대한건축학회 2003 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.23 No.2(구조계)

This paper presents an analytical model for structural members subject to cyclic inelastic load. The model, modified smooth model, can take into account the non-symmetrical behavior, the pinching effects, the axial load effect under the condition of biaxial moments. The analytical model was compared with other analytical method and experimental results. The fiber model was used to make comparison with the proposed model. The experimental results were selected from tests for hybrid structures consisted concrete and steel or precast and cast-in-place concretes. A good agreement was obtained with the proposed model.

양극성 장애의 우세삽화와 임상양상 간의 연관성 : 예비연구

서호준,우영섭,채정호,전태연,김광수,박원명 大韓神經精神醫學會 2007 신경정신의학 Vol.46 No.5

Objective : This study was performed to fmd clinical implications ofdistinctive pattems in bipolar patients with predominance of depressive episodes or manic episodes and to find possibilities to predict predominant episodes in bipolar disorder. Methods : The recmitment period was between 2002 and 2004, among inpatients who had received psychiatric treatment for bipolar disorder. The data about the number and the polarity of the past episodes were collected through medical records, and Sub-jects were divided into depressive or manic episode predominant groups. The two groups were compared regarding Sociodemo-graphic and clinical variables. Results : 74 patients met the inclusion criteria during the study period. 21 subjects were classified as depressive episode Pre-dominant group (depressive episode group), and 53 subjects as manic episode predominant group (manic episode group). There were no differences in sociodemographic data, the number of total episodes and hypomanic episodes, and onset age. However, 90.5% (n=19) subjects of depressive episode group showed depressive episode as first episode, and 88.7% (n=47) of manicepisode group experienced manic episode as first episode (χ²=42.13, df=1, P<0.00l). Psychotic symptomswere significantly more prominent in the manic episode group (χ²=6.85, df=1, P=0.009), and the number ofsubjects who had suicide attempt experience was higher in the depressive episode group (χ²=7.39, df=1, P=0.007). In the comparison of highest GAF scores during the past year, significant difference was found between the two group (p=0.046), as the mean scores of depressive epi-sode group was 68.337±7.47 and manic episode groupwas 71.51±5.42. Conclusion : The results ofthis study show that there are differences in clinical features according to predominant episodes in bipolar disorder. The type of onset episode appears to be related to predominant episode in this study, and it would be a valid Pa-rameter with therapeutic implication in bipolar patients.