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Lee, Hyeon Il,Kim, Mee Kum,Oh, Joo Youn,Ko, Jung Hwa,Lee, Hyun Ju,Wee, Won Ryang,Lee, Jin Hak The Korean Academy of Medical Sciences 2008 JOURNAL OF KOREAN MEDICAL SCIENCE Vol.23 No.3
<P>We performed this study to investigate the feature of rejection in porcine-to-rat corneal orthotopic transplantation and to evaluate the effect of cyclosporine and mycophenolate on the xeno-rejection. Orthotopic corneal transplantation was done at 91 Sprague-Dawley rats, and they were divided into 10 groups based on the combination of immunosuppressants including dexamethasone, cyclosporine, and mycophenolate mofetil. Graft survival was analyzed and grafted eyes were examined with Hematoxylin & Eosin and CD4 or CD8 staining. Enzyme-linked immunosorbent assays were done for interleukin-2 (IL-2), IL-4, IL-5, IL-10, and interferon (IFN)-γ in cornea, lacrimal gland, and cervical lymph nodes. The longest median survival of the immune suppressant group was 11.00±1.96 days, which showed no statistical differences compared with that of control (8.00±1.52 days). The neutrophils were prominent in the early phase but soon gave way to the monocytes. The number of CD8+ cells was higher than that of CD4+ cells. IL-2 and IFN-γ markedly increased at 10 to13 days in cornea, lacrimal glands, and cervical lymph nodes, which showed a decrease with immunosuppressants except in the cornea. In conclusion, cyclosporine and mycophenolate could not prevent the rejection in porcine to rat orthotopic corneal xenograft associated with infiltraton of CD8+ and innate immune cells.</P>
A subset of human rapidly self-renewing marrow stromal cells preferentially engraft in mice
Lee, Ryang Hwa,Hsu, Shu Ching,Munoz, James,Jung, Jin Sup,Lee, Na Rea,Pochampally, Radhika,Prockop, Darwin J. American Society of Hematology 2006 Blood Vol.107 No.5
<P>Controversies have arisen as to whether adult stem cells or progenitor cells from bone marrow can engraft into nonhematopoietic tissues in vivo. To resolve some of the controversies, we developed a highly sensitive polymerase chain reaction-based single nucleotide polymorphism (PCR-SNP) assay for competitive engraftment of mixtures of stem/progenitor cells. We used the assay to follow engraftment in immunodeficient mice of subpopulations of the stem/progenitor cells from human bone marrow referred to as either mesenchymal stem cells or marrow stromal cells (MSCs). The engraftment into adult mice without induced tissue injury was low and variable, but there was preferential engraftment of a subpopulation of rapidly self-renewing MSCs (RS-MSCs) compared with a subpopulation of slowly renewing MSCs (SR-MSCs). After intravenous infusion, there was a tendency for the cells to engraft into the hippocampal region that was previously designated a “vascular niche.” Migration assays suggested that preferential engraftment of RS-MSCs was in part explained by their expression of CXCR4 and CX3R1, the receptors for SDF-1 and fractalkine.</P>
증례(症例) : 후천성 면역 결핍증 (AIDS) 연관형 Kaposi 육종 1예
이선화 ( Sun Hwa Lee ),이민희 ( Min Hee Lee ),김상균 ( Sang Kyoon Kim ),유창우 ( Chang Woo Rheu ),백경현 ( Kyung Hyun Paeck ),정치량 ( Chi Ryang Chung ),이석 ( Seok Lee ),이흥범 ( Heung Bum Lee ) 전북대학교 의과학연구소 2004 全北醫大論文集 Vol.28 No.2
Kaposi 육종은 후천성 면역 결핍증 환자에서 가장 흔하게 발생하는 신생물이지만그 치료에 대해서는 최근까지도 명확한 기준이 정립되어 었지 않았다. 근래에는 AIDS 연관형 Kaposi 육종의 병인에 있어서 HHV-8의 감염이나 숙주의 면역 상태가 중요한 역할을 한다는 것이 밝혀지면서 nucleoside analogues reverse transcriptase inhibitor와 protease inhibitor를 병합 사용하는 적극적인 항 retrovirus 치료 (HAART)를 시도하는 경향이며, 그 외에도 다양한 방법들을 사용한 성공적인 치료 예들이 보고 되고 있다. 본 저자들은 국내에 흔하게 보고되지 않은 AIDS 연관형 Kaposi 육종의 증례를 진단하고 HAART와 foscarnet 그리고 고식적 방사선 치료를 병행하여 적극 적인 내과적 치료를 시도한 증례를 경험하였기에 문헌 고찰과 함께 보고하는 바이다. AIDS-related Kaposi`s sarcoma is one of the most common neoplasms in patients with acquired immunodeficiency syndrome. Recently, human herpesvirus-8 (HHV-8) and the immune status of the host have known to be closely related to the pathogenesis of AIDS-related Kaposi`s sarcoma. Therefore, highly active antiretroviral therapy (HAART) which includes nucleoside analogues reverse transcriptase inhibitor and protease inhibitor has been widely attempted in AIDS-related Kaposi`s sarcoma. Other successful treatment regimens including cytotoxic agents, interferon-α, thalidomide and so on have been reported. However, standard treatment option. has not been established yet. Hereby, we report a case of patient with AIDS-related Kaposi`s sarcoma who was treated by zidovudine, lamivudine and indinavir for underlying HIV infection, and intravenous foscarnet and palliative radiotherapy for local control of oropharyngeal Kaposi`s sarcoma with literature review.
Late endobronchial metastasis from rectal cancer that mimics a primary lung cancer.
Choi, Kyoung Hwa,Park, Seoung Ju,Min, Kyung Hoon,Kim, So Ri,Lee, Min Hee,Chung, Chi Ryang,Han, Hyo Jin,Lee, Heung Bum,Rhee, Yang Keun,Jin, Gong Yong,Chung, Myung Ja,Lee, Yong Chul American Lung Association 2011 American journal of respiratory and critical care Vol.183 No.1
Oh, Joo Youn,Kim, Mee Kum,Lee, Hyun Ju,Ko, Jung Hwa,Kim, Youngji,Park, Chan-Sik,Kang, Hee Jung,Park, Chung-Gyu,Kim, Sang Joon,Lee, Jin Hak,Wee, Won Ryang Blackwell Publishing Ltd 2010 Xenotransplantation Vol.17 No.2
<P>Oh JY, Kim MK, Lee HJ, Ko JH, Kim Y, Park CS, Kang HJ, Park CG, Kim SJ, Lee JH, Wee WR. Complement depletion with cobra venom factor delays acute cell-mediated rejection in pig-to-mouse corneal xenotransplantation. Xenotransplantation 2010; 17: 140–146. © 2010 John Wiley & Sons A/S.</P><P>Abstract: Background: </P><P>We have demonstrated earlier that porcine corneal xenografts underwent an acute cell-mediated rejection in mice despite the absence of T cells. In the present study, we investigated the effect of complement depletion by cobra venom factor (CVF) on the corneal xenograft rejection in a pig-to-mouse model.</P><P>Methods: </P><P>Porcine corneas were orthotopically transplanted into C57BL/6 (B6) and severe combined immunodeficiency (SCID) mice. For complement depletion, 25 <I>&mgr;</I>g of CVF (1 g/kg bodyweight) was injected intraperitoneally on the day before and 1, 3, 5, and 7 days after transplantation. Graft survival was clinically assessed by slit lamp biomicroscopy and the median survival time (MST) was calculated. The grafts were histologically evaluated serially after transplantation using antibodies against CD4, CD8, NK1.1, and F4/80.</P><P>Results: </P><P>The CVF treatment significantly prolonged the porcine corneal xenograft survival in both B6 (MST 9.4 vs. 15.5 days; P = 0.0011) and SCID mice (MST 16.4 vs. 20.5 days; P = 0.0474). Histologically, whereas macrophages and CD4<SUP>+</SUP> T cells were progressively infiltrated into porcine corneal grafts in CVF-untreated B6 mice, the infiltration by both cells was markedly delayed and decreased in the xenografts in CVF-treated B6 mice. Likewise, macrophage infiltration, which was prominent in rejected porcine xenografts in SCID mice, was also reduced in CVF-treated SCID mice.</P><P>Conclusions: </P><P>Our results suggest that complement depletion by CVF delayed, although did not prevent, an acute cell-mediated rejection in a pig-to-mouse corneal xenotransplantation.</P>
모르핀이 산화적 손상에 의한 신경아종 세포주 SH-SY5Y 의 세포고사에 미치는 영향
이창수,손용,윤재승,김태요,송윤강,정영표,하정량,최덕화,김정훈 대한마취과학회 2000 Korean Journal of Anesthesiology Vol.38 No.2
Background: The effect of opioids on nitric oxide (NO)- and peroxynitrite-induced neuronal cell death is largely unknown. In the present study, we examined the effect of morphine on NO- and per- oxynitrite-induced cell death using a human neuroblastoma SH-SY5Y cell line, which abundantly expre- sses μ, 8, κ-opioid receptors. Methods: The cultured cells were pretreated with morphine and exposed to 3-morpholinosydnonimine (SIN-1) that simultaneously generates NO and superoxide, thus possibly forming peroxynitrite. The cell damage was assessed by using MTT assay and crystal violet staining. Morphological nuclear changes and enzymatic evidences of apoptosis of the cells after exposure to SIN-1 for 24 hours were evaluated by using 4', 6-diamidino-2-phenylindole (DAPI) staining and the measurement of pro-apoptotic protease (caspase-3) activity, respectively. Levels of reduced glutathion (GSH) were measured by monochlo- ronimane (MCB) assay. Results: Pretreatrnent of SH-SY5Y with morphine significantly inhited the apoptotic cell death. Morphine also inhibited SIN-1-induced caspase-3 (pro-apoptotic protease) activity in a dose-dependent ma1nner. However, naloxone (20 μM) could not antagonize completely the effect of morphine in SIN- 1-induced cell death. Pre-administered GSH and N-acetylcysteine (NAC) have been found to protect SIN-induced apoptosis, and the neuroblastoma cells treated with morphine had significantly elevated the levels of GSH. Conclusions: The present study shows that morphine protects the human neuroblastoma cell line SH- SY5Y frorn peroxynitrite-induced apoptotic cell death through elevated GSH levels.The protective action of morphine seems to be associated with inhibition of the apoptotic pathway. However, it is suggested that morphine protects the cells possibly via other unknown mechanisms in addition to the activation of opioid receptors. ( Korean J Anesthesion 2000; 38: 356~364 )