침습성 아스페르길수스증 진단을 위항 갈락토만난 항원검사의 유용성 평가

송경호,이신원,장희창,전재현,박완범,박경운,박상원,오명돈,최강원 대한감염학회 2009 감염과 화학요법 Vol.41 No.2

Background : Invasive aspergillosis (IA) is an important cause of morbidity and mortality among immunocompromised patients. However, 1A is difficult to diagnose, especially in the aforementioned patient group. Recently, galactomannan assay (GMA) using enzyme immunoassay has been introduced in Korea. We evaluated its diagnostic usefulness in the diagnosis of 1A and we analyzed the results according to the underlying diseases. Materials and Methods : All patients who underwent GMA during the period from October 2007 to June 2008 were evaluated retrospectively. According to the criteria of European Organization for Research and Treatment of Cancer/Mycoses Study Group, IA was classified into four clinical categories: proven', probable', possible', and 'non' IA. Patients with 'proven' and 'probable' IA were used as the reference standards for IA. GMA was performed using Platelia Aspergillus EIA (Bio-Rad, Hercules, CA, USA). Optical density index ≥0.5 was considered positive. Results : Of the 144 patients who underwent GMA, two patients were classificed as proven' IA and sixteen patients were probable' 1A. Overall sensitivity, specificity, positive predictive value, and negative predictive value of the assay were 50% (95% confidence interval [C195), 26-74%), 88% (C195, 81-93%), 38% (CI95, 19-59%), and 93% (CI95, 86-97%), respectively. Among the 99 patients with underlying hematologic diseases, GMA showed 67% (C195, 35-90%) sensitivity and 89% (C195, 80-94%) specificity, whereas in 45 patients with underlying diseases other than hematologic diseases, sensitivity and specificity of the assay were 17% (CI95, 0-64%) and 87% (CI95, 73-96%), respectively. Conclusions : GMA showed high specificity, irrespective of the patient population. However, sensitivity of GMA was low and the assay was less sensitive in patients with underlying diseases other than hematologic diseases than in patients with hematologic diseases.

Morin exerts cytoprotective effects against oxidative stress in C2C12 myoblasts via the upregulation of Nrf2-dependent HO-1 expression and the activation of the ERK pathway

Lee, Moon Hee,Han, Min Ho,Lee, Dae-Sung,Park, Cheol,Hong, Su-Hyun,Kim, Gi-Young,Hong, Sang Hoon,Song, Kyoung Seob,Choi, Il-Whan,Cha, Hee-Jae,Choi, Yung Hyun UNKNOWN 2017 INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE Vol.39 No.2

<P>In the present study, we investigated the cytoprotective efficacy of morin, a natural flavonoid, against oxidative stress and elucidated the underlying mechanisms in C2C12 myoblasts. Our results indicated that morin treatment prior to hydrogen peroxide (H2O2) exposure significantly increased cell viability and prevented the generation of reactive oxygen species. H2O2-induced comet-like DNA formation and gamma H2AX phosphorylation were also markedly suppressed by morin with a parallel inhibition of apoptosis in C2C12 myoblasts, suggesting that morin prevented H2O2-induced cellular DNA damage. Furthermore, morin markedly enhanced the expression of heme oxygenase-1 (HO-1) associated with the induction and phosphorylation of nuclear factor-erythroid 2-related factor 2 (Nrf2) and the inhibition of Kelch-like ECH-associated protein 1 (Keapl) expression. Notably, these events were eliminated by transient transfection with Nrf2-specific small interfering RNA. Additional experiments demonstrated that the activation of the Nrf2/HO-1 pathway by morin was mediated by the extracellular signal-regulated kinase (ERK) signaling cascade. This phenomenon was confirmed with suppressed Nrf2 phosphorylation and consequently diminished HO-1 expression in cells treated with a pharmacological inhibitor of ERK. Collectively, these results demonstrated that morin augments the cellular antioxidant defense capacity through the activation of Nrf2/HO-1 signaling, which involves the activation of the ERK pathway, thereby protecting C2C12 myoblasts from H2O2,-induced oxidative cytotoxicity.</P>

Elevational Distribution of Breeding Bird Communities in Seoraksan National Park, Korea

Hwang, Hyun-Su,Lee, Jae-Kang,Eom, Tae-Kyung,Bae, Ho-Kyoung,Lee, Dong-Ho,Lim, Jong-Hwan,Jung, Sung-Cheol,Park, Chan-Ryul,Rhim, Shin-Jae Korean Society of Forest Science 2020 한국산림과학회지 Vol.109 No.1

In this study, the elevational distribution of breeding birds in Seoraksan National Park, Korea was investigated. Forty-six species of birds were documented from line transect surveys taken from Seorakdong at 230 m above sea level (a.s.l.) to the Daechungbong summit at 1708 m asl. Birdspecies richness and diversity were highest in Seorakdong and lowest at the Daechungbong summit. As elevation increased, bird species richness and diversity decreased, with a humped-shape trend being observed between 700 m and 1200 m a.s.l. Stepwise analyses revealed that breeding bird species diversity indices were significantly negatively correlated with elevation (r² = 0.327, P < 0.001) and positively correlated with vegetational coverage (r² = 0.324, P = 0.046). Higher elevations supported fewer birds than low and intermediate elevations. Projections at the local scale, including data on behavior and habitat use by birds, will be necessary for optimal conservation and management of the bird communities in Seoraksan National Park.

Elevational Distribution of Breeding Bird Communities in Seoraksan National Park, Korea

( Hyun-su Hwang ),( Jae-kang Lee ),( Tae-kyung Eom ),( Ho-kyoung Bae ),( Dong-ho Lee ),( Jong-hwan Lim ),( Sung-cheol Jung ),( Chan-ryul Park ),( Shin-jae Rhim ) 한국산림과학회(구 한국임학회) 2020 한국산림과학회지 Vol.109 No.1

In this study, the elevational distribution of breeding birds in Seoraksan National Park, Korea was investigated. Forty-six species of birds were documented from line transect surveys taken from Seorakdong at 230 m above sea level (a.s.l.) to the Daechungbong summit at 1708 m asl. Birdspecies richness and diversity were highest in Seorakdong and lowest at the Daechungbong summit. As elevation increased, bird species richness and diversity decreased, with a humped-shape trend being observed between 700 m and 1200 m a.s.l. Stepwise analyses revealed that breeding bird species diversity indices were significantly negatively correlated with elevation (r² = 0.327, P < 0.001) and positively correlated with vegetational coverage (r² = 0.324, P = 0.046). Higher elevations supported fewer birds than low and intermediate elevations. Projections at the local scale, including data on behavior and habitat use by birds, will be necessary for optimal conservation and management of the bird communities in Seoraksan National Park.

Slide Session : OS-IFD-07 ; Infectious Disease : In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus

( Myung Jin Lee ),( Kye Hyung Kim ),( Jong Youn Yi ),( Su Jin Choi ),( Chung Jong Kim ),( Nak Hyun Kim ),( Kyoung Ho Song ),( Pyoeng Gyun Choi ),( Ji Hwan Bang ),( Wan Beom Park ),( Eu Suk Kim ),( San 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus Myung Jin LEE1, Kye-Hyung KIM1, Jongyoun YI2, SuJin CHOI1, Chung-Jong KIM1, Nak- Hyun KIM1, Kyoung-Ho SONG1, Pyoeng Gyun CHOI1, Ji-Hwan BANG1, Wan Beom PARK1, Eu Suk KIM1, Sang-Won PARK1, Hong Bin KIM1, Nam Joong KIM1, Myoung- Don OH1 Seoul National University College of Medicine, Korea1, Pusan National University School of Medicine, Korea2 Background: Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease caused by a novel Bunyavirus, severe fever with thrombocytopenia syndrome virus (SFTSV). No effective antiviral therapy is proven yet, but clinical use of ribavirin (RBV) has been tried. We investigated the antiviral effect of RBV against SFTSV in vitro. Methods: To test for cytotoxicity of RBV, Vero cells were treated with different concentrations of RBV (3.90 to 500 μg/mL, two-fold dilution) and analyzed by cell viability MTS assay 48h post-infection. To determine antiviral activity of RBV against SFTSV, Vero cells were infected with SFTSV strain Gangwon/Korea/2012 at 100 TCID50 (50% tissue culture infective dose) per well in a 96-well plate, and RBV was added at the concentrations showing no or minimal cytotoxicity. Viral RNAs were extracted from the culture supernatants and quantifi ed using one-step real-time reverse transcription- PCR to amplify the partial large segment of SFTSV. Statistical analysis was done by one-way ANOVA with Tukey`s post hoc test. Results: Cytotoxicity due to RBV was not observed at RBV concentration =31.3 μg/ mL. Viral RNAs at 24h post-RBV treatment were reduced with increasing RBV concentrations (1-32 μg/mL), compared with those of mock-treated cells (P <0.01, Figure). Half maximal inhibitory concentration (IC50) of RBV was 3.69 μg/mL at 24h post-RBV treatment. Conclusions: Our study shows that RBV has antiviral effect against SFTSV in a dose-dependent manner. Further studies are required to evaluate the effi cacy of RBV in SFTS.

연속슬래브교량의 고유진동해석에 대한 수치모형

박제선,심도식,이정호,정경일 江原大學校 産業技術硏究所 1997 産業技術硏究 Vol.17 No.-

The problem of deteriorated highway concrete slab is very serious all over the world. Before making any decision on repair work, reliable non-destructive evaluation is necessary. One of the dependable methods is to evaluate the in-situ stiffness of the slab by means of obtaining the natural frequency. By comparing the in-situ stiffness with the one obtained at the design stage, the degree of damage can be estimated rather accurately. In this paper, the numerical modeling of vibration analysis to three span continuous reinforced concrete bridge with elastic intermediate support is presented.

개에서 발생한 다중심성 T 세포 림프종 증례

이기호,박형진,송근호,서경원 忠南大學校 獸醫科大學 附設 動物醫科學硏究所 2012 動物醫科學硏究誌 Vol.19 No.1

A 5-year-old Jindo dog (13.7 kg, intact female) with history of cervical mass was referred to the Veterinary Medical Teaching Hospital of Chungnam National University. Complete blood cell count showed mild anemia thrombocytopenia. And serum biochemistry showed increased ALP. On radiologic evaluation, multicentric lymph nodes' enlargement. Based on the findings, the dog was diagnosed as multicentric T-cell lymphoma. The dog was treated with LCOAP-short protocol, but survived 7 months since first finding.

등척성 운동 시 요추의 각도에 따른 중앙주파수와 토크의 특성

박경희,권오윤,장근,강성재,김영호 한국전문물리치료학회 2001 한국전문물리치료학회지 Vol.8 No.2

Fatigue is the decline in force produced as a result of prolonged muscle activity. Localized muscle fatigue can be identified by a shift toward low in the frequency components of the EMG signal, typically represented by a fall in the median frequency. Previous studies show that a shortened muscle developes a higher fatigue than elongated muscles. The purpose of this study was to investigate the time-related change of median frequency and torque during maximal isometric back extension exercises at different exercise angles (0˚, 12˚, 36˚, 72˚). Twenty healthy subjects (mean age = 24.35 ± 2.70) were evaluated in this study. Median frequency was extracted from the EMG signals by fast Fourier transform (FFT). Initial median frequency and the slope of median frequency change over time were computed from linear regression analysis. Pearson's product moment correlation was used to quantify the relationship between scope of median frequency and torque. The results were as follows: 1) Significant differences in y-intercepts of torque regression equation with respect to exercise angle were shown. However, there were no differences in the slopes of the median frequency and torque, and y intercept of the median frequency among exercise angles. 2) There was no significant correlation between slope of median frequency and torque. 3) But there was moderate correlation between median frequency and torque at each exercise angle. In conclusion, the exercise angle during maximal isometric back extension exercise is not a direct effect on slope of median frequency and torque. But results showed that median frequency and torque shift were highly correlated in all subjects.

고정화 세포를 이용한 황화수소 제거공정 최적화

박양호,손효진,김경주,권규혁,이중헌 한국공업화학회 2003 응용화학 Vol.7 No.1

In this study, the hybitd reactor has used to remove H_2S from the toxic gas. Since the oxidation and reduction reactors were combined, theri sizes were important to stabilize developed process. The optimal sizes of oxidation and reduction reactors were determined considering iron oxidation rate and H_2S removal rate. The optimal ratio of reactor size was 4 L for oxidation reactor to 1 L for reduction reactor.Immobilized Thiobacillus ferrooxidans has been developed to minimize the size of oxidation reactor. Since the immobilized cell enhanced the oxidation rate, it was possible to incrase H_2S removal efficiency. It was possible to incerase operation time because immobilized cell can survie lger than free cell.

정규 뇌파검사에서 볼수 있는 자연수면

박경신,한선호 大韓神經精神醫學會 1988 신경정신의학 Vol.27 No.4

The purpose of this study were to investigate the patterns of sleep appearances and to find out conditions for easier occurrence of natural sleep during routine EEG examination. The subjects of this study were randomly selected 200 patients who had undergone EEG examinations at Soonchunhyang University Hospital EEG laboratory. The results were as follows ; 1) Half of the cases(50%) showed sleep EEG patterns and 56% of them(56 cases) showed sleep stage Ⅱ patterns. 2) Sleep latency of those who reached only sleep Ⅰ was significantly longer than that of those who reached sleep stage Ⅱ. 3) Sleep was induced within the first 15 minutes of EEG records in 86.4% of all the sleepers. Afterwards, the sleep induction rate was abruptly decreased. 4) Ninety percent of the sleepers showed the EEG patterns of sleep stage Ⅱ within the first 15 minutes of sleep. 5) When the subjects were divided into two groups by the time of EEG recording, i.e, morning and afternoon groups, there was no difference in the rates of sleep induction and appearance of sleep stage Ⅱ. However, sleep latency was significantly shorter in the afternoon group. 6) EEG patterns of sleep stages 3 and stage 4 and REM sleep were not observed in any subjects studied.