난관배양액이 처녀발생유기된 돼지난포란의 체외발달에 미치는 영향

문승주,이경호,김호,김창렬,은대숙,김광현,나진수,김재홍 全南大學校 農業科學技術硏究所 1997 農業科學技術硏究 Vol.32 No.-

본 연구는 난관배양액이 돼지수정란의 체외발달에 미치는 효과를 규명키 위하여 수행하였다. 돼지 미성숙 난포란은 TCM-199, Ham's F-10 그리고 Whitten's 배양액에 10% 난포액과 호르몬(PMSG : 10IU/㎖, HCG : 10IU/㎖)을 첨가 20시간 배양하고 호르몬을 첨가하지 않는 배양액에서 20시간 추가 배양하여 총 40시간동안 배양하여 체외성숙을 유도하였다. 체외성숙후 0.1% hyaluronidase로 난구세포를 제거하고 15% FCS가 함유된 TCM-199으로 3회 세척하고 TCM-199에 15% FCS와 10% ethanol 혼합액에 세척한 난자를 옮겨 10분간 배양 처녀발생을 유기하였다. 처녀발생 6시간후 전핵형성율은 체외성숙배양액으로 TCM-199을 사용했을 때 56.4%, Ham's F-10의 경우 58.3%, Whitten's 배양액의 경우 74.0%를 보였다. 처녀발생 유기 48시간째 난할율은 TCM-199을 사용했을 때 45.7%, Ham's F-10에서 45.4%, Whitten's배양액에서 39.2%를 보였으며 세종류의 배양액에 POCM을 첨가 배양했을 때 TCM-199에 44.8%, Ham's F-10에서 45.4%, Whitten's배양액에서 43.7%로 나타났다. 처녀발생육 난자를 96시간 체외배양시킨 결과 상실배 발달율이 POCM을 첨가 했을 때 첨가하지 않은 시험구에 비하여 유의적으로 높았다(P<0.05) The effect of porcine oviductal conditioned medium(POCM) on in vitro development of chemically activated porcine oocytes was studied. Porcine oocytes were cultured in TCM-199, Ham's F-10 and Whitten's medium with hormonal supplements for 20h and 40h additional culture without hormonal supplements. After in vitro maturation, the denuded oocytes were washed 3 times with TCM-199 contaning 15%(v/v) ethanol to induce pathenogenetic activation. At 6h after activation, pronuclea formation rates were 56.4% in TCM-199, 59.3% in Ham's F-10 and 74.0% in Whitten's maturation medium. At 48h after activation, 45.7%, 45.4% and 39.2% of oocytes claved in TCM-199, Ham's F-10 and Whitten's culture medium, respectively. And 44.8%, 45.5% and 43.7% of oocytes were claved in TCM-199, Ham;s F-10 and Whitten's culture medium supplemented with POCM, respectively. The rates of moular were higher in culture medium with POCM than without POCM at 96h after activation.(P<0.05)

한우 c-fos 유전자의 염기서열 및 발현분석

유성란,정행진,정기철,이준헌,조규완,최재관,나기준,상병찬 한국동물자원과학회 2003 한국축산학회지 Vol.45 No.6

Cellular FOS(c-fos) protein is a transcription factor that forms heterodimers mostly with c-jun family and stimulates the transcription of genes containing AP-1 regulatory elements. This c-fos expression can control growth and differentiation of various precursor cells including myoblasts. The controls by c-fos gene have been identified for affecting skeletal muscle fiber traits which are the key determinants of meat quality in pigs. As a first step for identifying the relationship between c-fos gene and meat quality traits in cattle, we fully sequenced 1,443 bp of Hanwoo c-fos mRNA and analyzed expression patterns from various organs and muscle tissues. The sequence identities of Hanwoo c-fos with that of human, pig and mouse showed 89.8%, 93.3% and rib muscle from 7 organs and 9 different parts of muscles investigated. These results presented here can be used as a valuable marker for meat quality related traits in cattle with further verification.

Mechanical properties of (W,Ti)C and (W,Ti)C-NiAl₃ cermet consolidated by the high-frequency induction-heating method

Kim, W.,Suh, C.Y.,Roh, K.M.,Cho, S.W.,Na, K.I.,Shon, I.J. Elsevier Sequoia 2013 Journal of alloys and compounds Vol.568 No.-

In the case of cemented (W,Ti)C, Co is added as a binder for the formation of composite structures. However, the high cost of Co and the low corrosion resistance of the (W,Ti)C-Co cermet have generated interest in recent years for alternative binder phases. In this study, NiAl<SUB>3</SUB> was used as a binder and consolidated by the high-frequency induction heated sintering (HFIHS) method. The densification of both monolithic (W,Ti)C and (W,Ti)C-NiAl<SUB>3</SUB> cermet was accomplished within 3min. Highly dense (W,Ti)C and (W,Ti)C-NiAl<SUB>3</SUB> with a relative density of upto 99% were obtained within 3min by HFIHS under a pressure of 80MPa. The method was found to enable not only the rapid densification but also the prohibition of grain growth preserving the nano-scale microstructure. The average grain sizes of the sintered (W,Ti)C and (W,Ti)C-NiAl<SUB>3</SUB> were lower than 100nm. The addition of NiAl<SUB>3</SUB> to (W,Ti)C enhanced the toughness at the expense of the slight decrease in hardness. The hardness of (W,Ti)C and (W,Ti)C-NiAl<SUB>3</SUB> was significantly higher than that of (W,Ti)C-Co or (W,Ti)C-Ni. The fracture toughness and hardness values of (W,Ti)C, (W,Ti)C-5vol.%NiAl<SUB>3</SUB>, and (W,Ti)C-10vol.%NiAl<SUB>3</SUB> consolidated by HFIHS with a pressure of 80MPa and a induced current were 7.6+/-0.4MPam<SUP>½</SUP> and 2850+/-35kg/mm<SUP>2</SUP>, 8.5+/-0.3MPam<SUP>½</SUP> and 2610+/-37kg/mm<SUP>2</SUP>, 9.7+/-0.5MPam<SUP>½</SUP> and 2520+/-26kg/mm<SUP>2</SUP>, respectively.

Lithium ion capacitors fabricated with polyethylene oxide-functionalized polysilsesquioxane hybrid ionogel electrolytes

Na, W.,Lee, A.S.,Lee, J.H.,Hwang, S.S.,Hong, S.M.,Kim, E.,Koo, C.M. Pergamon Press 2016 ELECTROCHIMICA ACTA Vol.188 No.-

We present a new polyethylene glycol-functionalized polysilsesquioxane, a ladder-like structured poly(poly(ethylene oxide)-co-methacryloxypropyl)silsesquioxane) (PEO-SQ) hybrid-type polymer gelator to fabricate hybrid ionogel electrolytes for lithium ion capacitors. The organic-inorganic hybrid polymer gelator contains not only an inorganic ladder-like polysilsesquioxane backbone for the thermal and electrochemical stability, but also dual organic pendant groups consisting of free-dangling PEO chains and unsaturated methacryloxypropyl groups for enhanced lithium ion dissociation and thermal curing function, respectively. The successful crosslinking of 1M LiTFSI in N-butyl-N-methylpyrrolidinium bis(trifluoromethylsulfonyl)imide (BMPTFSI) ionic liquid with only a small amount of PEO-SQ hybrid crosslinker (5wt%) gave non-leaking ionogels with high thermal stability (@?400<SUP>o</SUP>C), large ionic conductivities, and excellent lithium ion capacitor C-rate performance and cyclability, comparable to those of neat ionic liquid electrolyte and superior over conventional organic ionogels fabricated with organic crosslinkers without PEO-moeities.

호흡기 관련 아데노바이러스의 진단 및 형 분석을 위한 Single tube multiplex polymerase chain reaction의 개발

나병국,김지희,신구철,이주연,이진수,강춘,김우주 국립보건연구원 2000 국립보건원보 Vol.37 No.-

조직구성 수성 망상증 1예

나한식,조경상,김종두,이승일,문철웅,이호영,장숙진 朝鮮大學校 附設 醫學硏究所 1989 The Medical Journal of Chosun University Vol.14 No.2

Histiocytic medullary reticulosis is a clinicopathologic syndrome characterized by its acute onset and relentless progression to death within a few months. The major clinical features are fever, malaise, weakness, weight loss, lymphadenopathy, hepatosplenomegaly, jaundice and purpura. The common laboratory findings are severe anemia, leukopenia, and thrombocytopenia. Histologically, bone marrow were infiltrated with atypical, neoplastic histiocytes, many of which had ingested large number of red blood cells, leukocyte, platelet. We experienced one case of HMR who was a 22 year old male admitted via ER with malaise, general weakness, fever and headache. The patient died often 11 days of illness. Death was due to massive bleeding and DIC thought to be due to pancytopenia secondary to massive blood element phagocytosis. So, we reported one case which considered compatible for HMR, with a few elementary reviewed literatures.

유해화학물질 대기확산 예측을 위한 RAMS 기상모델의 적용 및 평가 : CARIS의 바람장 모델 검증 Validation of the Operational Wind Field Generation System in CARIS

김철희,나진균,박철진,박진호,임차순,윤이,김민섭,박춘화,김용준 한국대기환경학회 2003 한국대기환경학회지 Vol.19 No.5

The statistical indexes such as RMSE(Root Mean Square Error), Mean Bias errer, and IOA(Index of agreement) are used to evaluate 3 Dimensional wind and temperature fields predicted by operational meteorological model RAMS(Regional Atmospheric Meteorological System) implemented in CARIS(Chemical Accident Response Information System) for the dispersion forecast of hazardous chemicals in case of the chemical accidents in Korea. The operational atmospheric model, RAMS in CARIS are designed to use GDAPS, GTS, and AWS meteorological data obtained from KMA(Korean Meteorological Administration) for the generation of 3-dimensional initial meteorological field. The predicted meteorological variables such as wind speed, wind direction, temperature, and precipitation amount, during 19~23, August 2002, are extracted at the nearest grid point to the meteorological monitoring sites, and validated against the observations located over the Korean peninsula. The results show that Mean bias and Root Mean Square Error are 0.9(m/s), 1.85(m/s) for wind speed at 10m above the ground, respectively, and 1.45(℃), 2.82(℃) for surface temperature. Of particular interest is the distribution of forecastion error predicted by RAMS with respect to the altitude; relatively smaller error is found in the near-surface atmosphere for wind and temperature fields, while it grows larger as the altitude increases. 0verall, some of the overpredictions in comparisons with the observations are detected for wind and temperature fields, whereas relatively small errors are found in the near surface atmosphere. This discrepancies are partly attributed to the oversimplified spacing of soil, soil contents and initial temperature fields, suggesting some improvement could probably be gained if the sub-grid scale nature of moisture and temperature fields was taken into account. However, IOA values for the wind field(0.62) as well as temperature field(0.78) is grater than the 'good' value criteria(>0.5) implied by other studies. The good value of IOA along with relatively small wind field error in the near surface atmosphere implies that, on the basis of current meteological data for initial fields, RAMS has good potentials to be used as a operational meteorogical model in predicting the urban or local scale 3-dimensional wind fields for the dispersion forecast in association with hazardous chemical releases in Korea.

Beta-Amyloid Oligomers Activate Apoptotic BAK Pore for Cytochrome c Release

Kim, J.,Yang, Y.,Song, S.,Na, J.H.,Oh, K.,Jeong, C.,Yu, Y.,Shin, Y.K. Biophysical Society ; Published for the Biophysica 2014 Biophysical journal Vol.107 No.7

In Alzheimer's disease, cytochrome c-dependent apoptosis is a crucial pathway in neuronal cell death. Although beta-amyloid (Aβ) oligomers are known to be the neurotoxins responsible for neuronal cell death, the underlying mechanisms remain largely elusive. Here, we report that the oligomeric form of synthetic Aβ of 42 amino acids elicits death of HT-22 cells. But, when expression of a bcl-2 family protein BAK is suppressed by siRNA, Aβ oligomer-induced cell death was reduced. Furthermore, significant reduction of cytochrome c release was observed with mitochondria isolated from BAK siRNA-treated HT-22 cells. Our in vitro experiments demonstrate that Aβ oligomers bind to BAK on the membrane and induce apoptotic BAK pores and cytochrome c release. Thus, the results suggest that Aβ oligomers function as apoptotic ligands and hijack the intrinsic apoptotic pathway to cause unintended neuronal cell death.

^19F(p, αγ)^16O 반응의 α-γ 각상관

박상태,김기동,이대원,정운혁,노태익,정성채,이종환,나상균 동의대학교 기초과학연구소 1994 基礎科學硏究論文集 Vol.4 No.1

The α-γ angular correlations in the ^19F(p, αγ)^16O reaction have been measured using a 500 keV VDG accelerator at E_p=340 keV and a CaF₂ taget prepared by evaporation in vaccum. The measurement of the α-γ angular correlation between the α-rays from the decay of ^20Ne into ^16O and the γ-rays from ^16O has been performed using a Si surface barrier detector and a NaI(TI) detector to determine the spin of the 13.196 MeV level in ^20Ne, and those of the 6.14 MeV and the ground level in ^16O, together with the orbital angular momentum of the emitted α's. The α-γ angular correlations in the ^19F(p, αγ)^16O reaction have been also calculated for the assumed spins of the 13.196 MeV level in ^20Ne, and those of the 6.14 MeV and ground level in ^16O. The spins of the levels under condsideration have been determined by comparison of the calculated values with experimental value. The result makes a good agreement to Arnold's data.

Electron-beam-induced reduced graphene oxide as an alternative hole-transporting interfacial layer for high-performance and reliable polymer solar cells

Kwon, S.N.,Jung, C.H.,Na, S.I. Elsevier Science 2016 ORGANIC ELECTRONICS Vol.34 No.-

<P>We demonstrate an eco-friendly, simple, and cost-effective method for manufacturing reduced graphene oxide (GO) induced with electron-beam irradiation, and we investigate the feasibility of the electronbeam-induced reduced GO (ERGO) as a hole-transporting interfacial layer in polymer solar cells (PSCs). In addition, the chemical composition, conductivity, work-function, and morphology of ERGOs with various absorbed doses were systematically investigated. The analytical results revealed that the reduced GO (RGO) was successfully prepared using electron-beam irradiation, and the electrical conductivity of ERGO was increased (up to 18.3 S/cm) with increasing the amount of absorbed dose. The PSCs with the ERGO as a hole-transporting interfacial layer exhibited comparable cell performance (3.52 +/- 0.08% of power conversion efficiency) to that of the conventional PSCs with the poly(3,4-ethylenedioxythiophene): polystyrene sulfonate (PEDOT:PSS), and they had better stability than the PEDOT:PSS-based PSCs. From the results, we confirmed that electron-beam irradiation is an effective approach to prepare the RGO, and the ERGO was preferable to the PEDOT:PSS for high-performance and stable PSCs. (C) 2016 Elsevier B.V. All rights reserved.</P>