Identification of Genes Modulated by High Extracellular Calcium in Coculture of Mouse Osteoblasts and Bone Marrow Cells by Oligo Chip Assay

Kim, Hyung-Keun,Song, Mina,Jun, ji-Hae,Woo, Kyung-Mi,Kim, Gwan-Shik,Baek, Jeong-Hwa The Korean Academy of Oral Biology 2006 International Journal of Oral Biology Vol.31 No.2

Calcium concentration in the bone resorption lacunae is high and is in the mM concentration range. Both osteoblast and osteoclast have calcium sensing receptor in the cell surface, suggesting the regulatory role of high extracellular calcium in bone merabolism. In vitro, high extracellular calcium stimulated osteoclastogenesis in coculture of mouse osteoblasts and bone marrow cells. Therefore we examined the genes that were commonly regulated by both high extracellular calcium and 1,25(OH)_(2)vitaminD_(3)(VD3) by using mouse oligo 11 K gene chip. In the presence of 10 mM[Ca^(2+)]e or 10 nM VD3, mouse calvarial osteoblasts and bone marrow cells were co-cultured for 4 days when tartrate resistant acid phosphatase-positive multinucleated cells start to appear. Of 11,000 genes examined, the genes commonly regulated both by high extracellular calcium and by VD3 were as follows; 1) the expressions of genes which were osteoclast differentiation markers or were associated with osteoclastogenesis were up-regulated both by high extracellular calcium and by VD3; trap, mmp9, car2, ctsk, ckb, atp6b2, tm7sf4, rab7, 2) several chemokine and chemokine receptor genes such as sdf1, scya2, scyb5, scya6, scya8, scya9, and ccr1 were up-regulated both by high ectracellular calcium and by VD3, 3) the genes such as mmp1b, mmp3 and c3 which possibly stimulate bone resorption by osteoclast, were commonly up-regulated, 4) the gene such as c1q and msr2 which were related with macrophage function, were commonly down-regulated, 5) the genes which possibly stimulate osteoblast differentiation and/or mineralization of extracellular matrix, were commonly down-regulated;slc8a1, admr, plod2, lox, fosb, 6) the genes which possibly suppress osteoblast differentiation and/or mineralization of extracellular matrix, were commonly up-regulated;s100a4, npr3, mme, 7) the genes such as calponin 1 and tgfbi which possibly suppress osteoblast differentiation and/or mineralization of extracellular matrix, were up-regulated by high extracelluar calcium but were down-regulated by VD3. These results suggest that in coculture condition, both high extracellular calcium and VD3 commonly induce osteoclastogenesis but suppress osteoblast differentiation/mineralization by regulating the expression of related genes.

중앙아시아 벽화 보존처리(1) : 벽화(본4074, 본4096)의 상태조사

강형태,이용희,유혜선,김연미,조연태,시고 아오키,노리코 야마모토,켄타로 오바야시 국립중앙박물관 2001 박물관보존과학 Vol.3 No.-

국립중앙박물관은 용산 새 박물관의 전시 유물로 선정된 중앙아시아 벽화의 연차적인 보존처리를 위해 “독립행정법인 일본 도쿄문화재연구소’’와 공동연구를 모색하게 되었다. 2001년에 본4074, 본4096 작은 벽화편 2점의 보존처리를 시작하면서 벽화의 균열 박락 등 손상상태, 구조와 구성재료, 이전 보존처리에서 적용된 재료와 방법에 대한 기초조사를 실시하였다. 조사결과 본4074, 본4096 2점의 벽화는 흙과 지푸라기를 반죽하여 만든 벽체에 석고를 발라 바탕으로 하고 그 위에 채색을 한 것으로 나타났으며 벽체 속에 포함된 지푸라기의 방사선 탄소연대측정 결과 본 벽화는 10세기 말에서 13세기 초에 만들어진 것으로 나타났다. 또한 X-선회절 분석 결과 화면의 흰색 바탕은 gypsum[Ca(SO₄)·2H₂O]과 CaSO₄, Calcite(CaCO₃)가 적색계통은 연단(鉛丹:Pb₃O₄)과 led arsenate[Pb(As₂O₆)], 녹색계통은 Cuprite(Cu₂O)와 arsenolite(As₂O₃), arsenic oxide(As₂O₄) 등이 사용된 것으로 조사되었다. This article is about a joint project carried out by the National Museum of Korea and the Tokyo Cultural Properties Research Institute for the conservation of central Asia Wall painting that has been selected for the exhibition at the new Seoul National Museum of Korea at Yongsan. The investigation of the wall painting revealed very useful information. This includes the condition of the object, and the identification of evident damage, such as cracks, loss of pigment, plus materials and methods employed during the object's creation, as well as previous conservation treatment. The object was mainly made by applying plaster to the body (wall) that consisted of a mixture of soils and rice straws. Then, on the surface of the wall-painting, pigments were used to draw and to colour it. As a part of the investigation, radiocarbon dating was conducted using straw samples taken from the object. The result indicates that the object is probably dated form between the end of the 10th Century and the beginning of the 13th Century. The result of X-ray diffraction also revealed the composition of the pigments used on the surface. These are 1. gypsom[Ca(SO₄)·2H₂O], CaSO₄ and Calcite(CaCO₃) and Calcite(CaCO₃) that were used for the white background. 2. Pb₃O₄ and led Arsenate [Pb(As₂O₆) that were used for the red colouring. 3. Cuprite(Cu₂O), Arsenolite(As₂O₃) and Arsenic Oxide(As₂O₄) that were used for the green colouring.

황남대총 98호분 출토 유리의 과학적 분석

조경미,유혜선,강형태 국립중앙박물관 1999 박물관보존과학 Vol.1 No.-

황남대총 98호분 북분과 남분에서 출토된 유리시료 40점에 대한 과학적인 분석을 실시하였다. 유리의 성분조성은 주사전자현미경-에너지분산형분광기(SEM-EDS)를 사용하여 정량분석 하였고 다변량해석법을 통하여 시료를 분류하였다. 그 결과 시료 40점 모두 Na₂O를 약 20%정도 함유한 소다-석회(Na₂O CaO-SiO₂)유리임을 확인하였으며, 다시 5개 주성분(SiO₂, AI₂O₃, Na₂O, CaO, K₂O)으로 다변량해석[주성분분석(PCA)]을 실시한 결과 2개의 群으로 분류되었다. I 群에 포함된 시료는 Al₂O₃의 농도가 9.7%로 높고 CaO의 농도는 2.2%인데 비하여 Ⅱ群에서는 각각 3.2%,4.9%의 범위로 나타났다. 특히 시료 No.12의 노란 색으로 편석된 부분을 미소부위 XRD로 분석한 결과 PbSnO₃임을 국내 최초로 확인할 수 있었다. 鉛을 함유한 시료 중 No.12, 17은 열이온화질량분석기(TIMS)로 납동위원소비를 측정하였고 선형판별식 분석법(SLDA)을 이용하여 납의 산지를 추정하였다. 그 결과 각각 중국 남부, 한국 남부의 납광석을 사용한 것으로 나타났다. Elemental analysis of 40 glass samples from the Northern Tomb and the Southern Tomb of Hwangnam-daechong No. 98 was performed. Fourteen compositions of each sample were analyzed quantitatively by SEM-EDS and glass samples were classified by multivariate analysis such as PCA. All of 40 samples were confirmed to be Na₂O CaO-SiO₂ system with about 20% of Na₂O. Samples were classified into two groups by doing PCA on concentrations of 5 major elements (SiO₂, AI₂O₃, Na₂O, CaO, K₂O). Samples included in group I showed the concentration of AI₂O₃ is about 9.7% and that of CaO, about 2.2%. In group II, concentration of Al₂O₃ is about 3.2% and that of CaO, about 4.9%. Especially yellow grains embedded in sample No.12 were shown to be PbSnO₃ by micro XRD, which was the first coloring material ever found in Korea. Lead isotope ratios of samples No.12 and No.17 which contained lead were measured by TIMS. The origin of lead was traced by means of multivariate analysis such as SLDA. The result showed that lead from southern China and southern Korea had been used for making glass.

형광 in situ hybridization 방법에 의한 c-myc 유전자의 전위 및 증폭에 관한 연구

김경채,박선화,김강련,김미영,전용혁 대한해부학회 1994 Anatomy & Cell Biology Vol.27 No.3

Aluminum응집제들에 의한 금속이온들의 응집에 관한 연구

김미향,최범석 慶熙大學校 材料科學技術硏究所 1999 材料科學技術硏究論集 Vol.12 No.-

Coagulation efficiencies of inorganic ions by aluminum based coagulants including alum, PAC, PACS were studied. Pb, As, Cu, Hg ions shows good coagulation efficiences with all the coagulants. Residual amount of Hg is also decreased by the precipitation as a hydroxide. The maximum coaulation efficiency is observed at the neutral pH and the efficiency is decreased with lowering pH, except Se. The coagulation efficiencis are decresed with an order of PACS, PAC, and alum. Cr, Cd shows lower coagulation efficiencies and Ni and Mn are not coagulated with all the coagulants.

정신지체장애아의 구강건강실태에 관한 조사연구

김미형 광주보건대학 2004 論文集 Vol.29 No.-

The purpose of this study was to obtain the basic data provide continuous and active dental treatment for the mentally handicapped children. The author studied on the dental caries experience and caries activity test in the mentally handicapped children who were housed by rehabilitation school in Kwang-ju. Correlation coefficiency was calculated between the caries experience and caries activity test for every children. The obtained results are as follows. 1. The mentally handicapped children showed remarkably high score in DMFT index. (The DMFT Index score was 5.26±4.11.) 2. Correlation coefficiency was reveled very high score between ft index and dmft index of the mentally handicapped children(P<0.01). 3. In the correlation coefficiency between the caries experience and modified snyder test, DMFT index of the mentally handicapped children was showed remarkably high score(P<0.01).

치과위생사 활용방안에 관한 조사연구

김미형 광주보건대학 1995 論文集 Vol.20 No.-

In order to collect the necessary information for the utilizable program of dental hygienists, the author questionnaired about 155 future dental hygienists who are sophomore dept. of dental hygiene in Kwang-ju and Jeon-nam health college. The obtained results were as follows: 1. 57.71 percent of the respondents had positive response about professional prospect and 85.71 percent of positive respondents selected the item because of taking a job easily. 2. 92.26 persent of the respondents desired to the opinion to develop the chair-side dental assistant institution as a new dental manpower in Korea. 3. 98.06 percent of the respondents answered if the chair-side dental assistant institution is developed, it is necessary for taking dental assistant share of the responsibilities of dental hygienist. 4. 77.24 percent of the respondents agrce to the opinion to expand the responsibilities of dental hygienist to the early treatment for dental caries and gingival disease. 5. 98.06 percent of the respondents answered that it is necessary for dental hygienist to be stationed dental clinic in elementary school. 6. 78.06 percent of the respondents wanted to work as long as their lives if the conditions are allowed.

Monocrotaline으로 유발시킨 흰쥐 폐고혈압에 대한 Flunarizine의 약리작용

안형수,이영미 同德女子大學校 1997 同大論叢 Vol.27 No.1

Monocrotaline treatment (60mg/kg, i.p.) in normotensive rats resulted the indications and related toxicities of pulmonary hypertension, including the increase of hematocrit value, right ventricle hypertropy, pulmonary edema and lethality, after 4 weeks later. However, daily administrtion of flunarizine (5mg/kg, p.o.) for 4 weeks seemed to decrease the above responxes, and also there was no significant change on the systemic mean arterial blood pressure and heart rate. In the anesthetized rats, hypotensive effect induced by isoproterenol bolus injection was signficantly inhibited in flunarizine-treated pulmonary hypertensive rats, comparing to the untreated rats, even if those by acetylcholine, adenosine and ATP were not. In the isolated pulmonary artery experiments, vasorelaxation induced by isoproterenol, but not acetylcholine, adenosine or ATP, were also selectively inhibited in flunarizine treated rats, comparing to the control rats. Also vasoconstriction induced by norepinephrine were increased in flunarizine treated rats comparing to the control rats, but those by KCI were not. However, pretreatment of flunarizine did not significantly affected on the vasodilation induced by acetylcholine, isoproterenol, adenosine or ATP and on the vasoconstriction induced by norepinephrine in isolated pulmonary artery of the normal rats. These results suggest that flunarizine will decrease the pathogenesis of pulmonary hypertension through the change of α- and β-adrenergic receptor activity in pulmonary artery due to the up-regulation of voltage dependent Ca++ channels.

GMM을 이용한 통화론적 환율결정모형의 추정

金美亨 全北産業大學校 産業經營硏究所 1997 産業經營硏究 Vol.5 No.-

This Article attempts to forecast monthly won-dollar exchange rates using GMM. The study addresses several issues. The first issue is whether Bilson's monetary exchange rate model estimated with OLS and GMM outperforms a random walk model. The second one is whether the different exchange rate regimes such as the monetary basket system and the market average exchange rate system that is a floating exchange rate system with a daily exchange rate change limit influence the goodness-of-fit of the monetary model. The third one is whether the size of the daily exchange rate change limit causes the forecasting ability of the models to differ. The fourth one is whether the size of the estimation window affects the forecasting power. The sample period is from January 1970 through June 1996. The results can be summarized as follows. First, Bilson's monetary exchange rate model estimated with GMM outperforms a random walk model in out-of-sample forecasting power. The forecasting ability of the monetary model estimated with OLS is, however, confounding. It is better than random walk model in the criteria of MAE but worse in RMSE. Second, exchange rate volatility has significantly decreased after monetary exchange rate system has changed to market average exchange rate system. The monetary exchange rate model estimated with GMM, however, is a good statistical model in two different exchange rate regimes respectively. Third, the forecasting ability of the exchange rate model differs depending on the size of the daily exchange rate change limit. The bigger a change limit, the bigger a forecasting error based on RMSE and MAE. Most of time, the monetary model outperforms a random walk model. Fourth, extending the estimation period, the forecasting error increases. It indicated that the coefficients of the monetary model are time-variant.

Ciprofloxacin의 돼지 호흡기 감염증 및 설사증에 대한 치료효과

전무형,김지영,채미경,서상희,김명철,박창식,김태용,윤효인 충남대학교 수의과대학 동물의과학연구소 2003 動物醫科學硏究誌 Vol.11 No.-

To evaluate the clinical efficacy of ciprofloxacin (CFX) on treatment of the pigs with respiratory diseases or diarrhea, CFX at various dosages were administered intramuscularly to the diseased pigs selected from the pig farms located in Chungnam province. One hundreds and fourteen heads of pigs with respiratory diseases were divided into five groups and CFX at 0.25, 0.5, 1.0 ( = 2.5mg CFX/Kg B.W.) and 2.0 doses were administered i.m. for 5 days. The recovery rates were found 31.8%, 73.9%, 91.3% and 92.0%, respectively, being higher than that of the untreated control group (19.0%). The mortality of the treated groups were 0%, that is lower than that of the control (9.5%). Seven kinds of bacteria were isolated from the respiratory organs of 86 pigs, and 34 (39.5%) of 86 pigs showed the complicated infection. In analyzing by the pigs infected with a single pathogen as P. multocida, A. pleuropneumonia, Staphylclcoccus spp. or Mycoplasma spp., the recovery rates were 0∼66.6% at 0.25 dose. 66.6∼100% at 0.5 dose and 100% in all of 4 pathogens at 1.0 to 2.0 doses. At 0.25 dosage the pigs infected with A. pleuropneumonia showed the lowest recovery rate. One hundreds and twenty two heads of pigs with diarrhea were divided into five groups and CFX at 0.25, 0.5, 1.0 ( = 2.5mg CFX/Kg B.W.) and 2.0 doses were administered i.m. for 5 days. The recovery rates were 29.1%, 40.0%, 80.0% and 88.0%, respectively. The mortality of the treated groups were 0-8.3%, being much lower than that of the control. Four kinds of bacteria were isolated from the fecal specimens of 94 pigs, and 84 (89.0%) of 94 pigs showed the complicated infection.