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茵蔯蒿가 흰쥐 肝조직내 Na^+·K^+ -ATPase 活性에 미치는 영향
金柱賢,申國鉉,趙台淳 成均館大學校 科學技術硏究所 1987 論文集 Vol.38 No.2
In order to investigate the effects of Artemisia on bile flow, the bile volume and the activities of Na^+·K^+-ATPase in liver plasma membrane and liver homogenate were examined in rats orally administered methanol extract of Artemisiae messer-schmidtiana Besser var. viridis Besser for 3 days. In the case of bile juice, the flow rate was increased 30∼70%. The activities of Na^+·K^+-ATPase was increased to 2.5 fold in liver homogenate and 2.1 fold in liver plasma membrane. On the other hand activities of Mg^++-ATPase was slightly increased in both liver homogenate and liver plasma membrane.
Seong, Jin Hyo,Kim, Dong Kie,Shin, Kwang Yong,Kim, Byung Ock,Kim, Myong Soo,Kim, Mi Kwang,Kook, Joong Ki 대한구강보건학회 2002 大韓口腔保健學會誌 Vol.26 No.4
본 연구는 치주질환과 관련된 병원성 세균 중 그람 음성 협기성 세균 종인 Prevorella intermedia (P. intermedia)와 Prevotella nigrescens (P. nigrescens)를 동시에 동정 및 검출할 수 있는 중합효소연쇄반응 프라이머를 개발하기 위하여 시행하였다. 모든 세균 종에 있어서 165 라이보송 RNA의 염기서 열에는 잘 보존된 부위와 종에 따라 다양한 부위가 있다. 이러한 특성을 이용하여 P. nigrescens와 P. intermedia의 알려진 165라이보솜 RNA의 염기서 열을 분석하여 두 세균 종에 공동으로 존재하는 하나의 프라이머와 각각의 세균 종에 특이하게 존재하는 다른 프라이머를 설계 및 제작하였으며, 이때 설계된 프라이머의 이름을 각각 Pin-F, Pi-R 및 Pn-R이라고 명명하였다. 이들의 종 특이성을 조사하기 위해 두 균주의 P. intermedia와 P. nigrescens지놈 DNA를 추출하여, 종 특이성을 중합효소연쇄반응법으로 관찰하였다. 이때 구강 내 6가지 다른 세균 종에서 같은 방법으로 중합효소연쇄반응을 실시한 결과 P. nigrescens와 P. intermedia 두 종만을 특이하게 검출함을 알 수 있었다. 또한 민감도를 조사하기 위해 두 세균 종에서 추출한 지놈 DNA를 4 ng에서 4 fg까지 희석하여 중합효소연쇄반응을 실시한 결과 400 fg의 지놈 DNA까지도 검출할 수 있었다. 이러한 결과를 종합할 때 이들 Pin-F, Pi-R 및 Pn-R프라이머 쌍은 P. inermedia와 P. nigrescens를 동시에 종특이적이며, 민감도 높게 검출 및 동정할 수 있을 것으로 사료된다.
Kim, Youngsoo,Kim, Seong Hun,Kim, Kook Hwan,Chae, Sujin,Kim, Chanki,Kim, Jeongjin,Shin, Hee-Sup,Lee, Myung-Shik,Kim, Daesoo IRL Press 2015 Human molecular genetics Vol.24 No.25
<P>Really interesting new gene (RING) finger protein 170 (RNF170) is an E3 ubiquitin ligase known to mediate ubiquitination-dependent degradation of type-I inositol 1,4,5-trisphosphate receptors (ITPR1). It has recently been demonstrated that a point mutation of <I>RNF170</I> gene is linked with autosomal-dominant sensory ataxia (ADSA), which is characterized by an age-dependent increase of walking abnormalities, a rare genetic disorder reported in only two families. Although this mutant allele is known to be dominant, the functional identity thereof has not been clearly established. Here, we generated mice lacking <I>Rnf170</I> (<I>Rnf170<SUP>−/−</SUP></I>) to evaluate the effect of its loss of function <I>in vivo</I>. Remarkably, <I>Rnf170<SUP>−/−</SUP></I> mice began to develop gait abnormalities in old age (12 months) in the form of asynchronous stepping between diagonal limb pairs with a fixed step sequence during locomotion, while age-matched wild-type mice showed stable gait patterns using several step sequence repertoires. As reported in ADSA patients, they also showed a reduced sensitivity for proprioception and thermal nociception. Protein blot analysis revealed that the amount of Itpr1 protein was significantly elevated in the cerebellum and spinal cord but intact in the cerebral cortex in <I>Rnf170<SUP>−/−</SUP></I> mice. These results suggest that the loss of <I>Rnf170</I> gene function mediates ADSA-associated phenotypes and this gives insights on the cure of patients with ADSA and other age-dependent walking abnormalities.</P>
Kim, Eun Tae,Lee, Sang Suk,Lee, Ji Hoon,Jeong, Jin Suk,Lee, Shin Ja,Jeong, Joon,Park, Jong Kook,Park, Beom Young,Kim, Sang Bum,Jeong, Ha Yeon,Ki, Kwang Seok,Choi, Chang Weon,Kim, Chang Hyun,Kim, Jin W Asian Australasian Association of Animal Productio 2018 Animal Bioscience Vol.31 No.9
Objective: This study was done to evaluate the effect of sodium stearoyl-2-lactylate (SSL) supplementation in a total mixed ration (TMR) on the lactation performance, blood parameters, and economic efficacy of mid-lactation Holstein cows. Methods: Twenty-four cows (body weight $647{\pm}11.7kg$) were randomly divided into 4 treatment groups, with six cows per group. The dietary treatments were as follows: basal diet (CON); CON+17.5 g of top dressed SSL (treatment [TRT] 0.05); CON+35 g of SSL (TRT 0.1); and CON+70 g of SSL (TRT 0.2) per 35 kg TMR. Results: The highest level of SSL supplementation (TRT 0.2) significantly improved milk yield during the second period compared to the TRT 0.05 group (5 to 8 wks; 33.28 vs 31.09 kg/d), during the third period compared to both the CON and TRT 0.05 groups (p<0.05) (9 to 13 wks; 32.59 vs 30.64 and 30.01 kg/d) and during the overall experimental period compared to both the CON and TRT 0.05 groups (p<0.05) (1 to 13 wks; 33.43 vs 32.06 and 31.40 kg/d), respectively. No negative effects on hematological or biochemical parameters were observed due to SSL supplementation. Considering both the milk fat and protein content, the total milk price was set at 1,073.60 (TRT 0.05), 1,085.60 (TRT 0.1), 1,086.10 (TRT 0.2), and 1,064.20 (CON) won/L, with consequent total milk profits of -1.7%, 5.4%, and 3.5% for the TRT 0.05, TRT 0.1, and TRT 0.2 diet, respectively, compared to those in the CON diet. Conclusion: The milk sales revenue related to SSL supplementation of the TRT 0.1 diet was increased by up to 5.4% compared to the milk sales revenue of the CON diet. Therefore, 0.1% SSL supplementation might be effective and profitable during the mid-lactation period of cows, without producing adverse effects.
Kim, Si Hyun,Shin, Jeong Hwan,Mok, Jeong Ha,Kim, Shine Young,Song, Sae Am,Kim, Hye Ran,Kook, Joong-Ki,Chang, Young-Hyo,Bae, Il Kwon,Lee, Kwangha Hindawi Publishing Corporation 2014 BioMed research international Vol.2014 No.-
<P><I>Introduction</I>. The aim of this study was to differentiate between <I>Candida famata</I> and <I>Candida guilliermondii</I> correctly by using matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) and gene sequencing. <I>Methods</I>. Twenty-eight <I>Candida</I> strains from blood cultures that had been identified as <I>C. famata</I> (<I>N</I> = 25), <I>C. famata</I>/<I>C. guilliermondii</I> (<I>N</I> = 2), and <I>C. guilliermondii</I> (<I>N</I> = 1) by the VITEK 2 system using the YST ID card were included. We identified these strains by MALDI-TOF MS and gene sequencing using the 28S rRNA and <I>ITS</I> genes and compared the results with those obtained by the VITEK 2 system. <I>Results</I>. All 28 isolates were finally identified as <I>C. guilliermondii.</I> Sequencing analysis of the 28S rRNA gene showed 99.80%–100% similarity with <I>C. guilliermondii</I> for all 28 strains. The <I>ITS</I> gene sequencing of the strains showed 98.34%–100% homology with <I>C. guilliermondii.</I> By MALDI-TOF, we could correctly identify 21 (75%) of 28 <I>C. guilliermondii</I> isolates. <I>Conclusion</I>. We should suspect misidentification when <I>C. famata</I> is reported by the VITEK 2 system, and we always should keep in mind the possibility of misidentification of any organism when an uncommon species is reported.</P>
Genome Sequence of Myroides injenensis M09-0166T, Isolated from Clinical Specimens
Kim, D.-S.,Paek, J.,Shin, J. H.,Kim, D.-W.,Jung, M. Y.,Kim, R. N.,Sin, Y.,Kook, J.-K.,Nam, S.-H.,Kim, A.,Kang, A.,Park, H.-S.,Choi, S.-H.,Chang, Y.-H. American Society for Microbiology 2012 Journal of Bacteriology Vol.194 No.10