Ki-67 labeling index as a prognostic marker in advanced stomach cancer

Sang Hyuk Seo,Kwang Hee Kim,Sang Hoon Oh,Yunseon Choi,Ki Jung Ahn,Ji Young Lee,Sang Min Lee,박지선,Woo Gyeong Kim 대한외과학회 2019 Annals of Surgical Treatment and Research(ASRT) Vol.96 No.1

Purpose: Proliferation marker Ki-67 is widely used in cancer prognosis prediction. We tried to investigate the role of Ki-67 as a prognostic factor in stomach cancer after surgery in this study. Methods: We retrospectively evaluated 251 patients who underwent curative resection for gastric cancer from 2010 to 2015. In pathologic examination, Ki-67 labeling index was defined as the percentage of Ki-67 antigen positive cells. Prognostic significance of Ki-67 for gastric cancer was evaluated. Disease-free survival (DFS) was assessed as a primary end-point. Results: The median follow-up period was 28.0 months. Thirty-one patients (12.4%) showed Ki-67 labeling index (LI) lower than 25%. Sixty-eight patients (26.6%) showed recurrence during follow-up period. Recurrence was associated with Ki- 67 LI level (≤25%, P = 0.016), and lymph node metastasis status (P = 0.002). High Ki-67 LI level (>25%) was also related to p53 positivity (P < 0.001) and poorly cohesive type (P = 0.002). The 3-year DFS was 69.4%. Low Ki-67 LI level (≤25%) was related with low DFS (47.6% vs. 72.6%, P = 0.016). T stage (P < 0.001), N stage (P = 0.006), lymphovascular invasion (P = 0.010), and neuronal invasion (P = 0.001) also affected the DFS. In addition, T stage (P = 0.03) and Ki-67 LI (P = 0.035) were independent prognostic factors for DFS. In patients treated with adjuvant chemotherapy (n = 239, 93.4%), low Ki-67 (≤25%) was a poor prognostic factor for DFS (P = 0.013). Conclusion: Low Ki-67 LI predicts high rate of progression and low DFS of stomach cancer. Ki-67 LI can be a predictive marker in resected stomach cancer treated with surgery and adjuvant chemotherapy.

Cell proliferation and neuroblast differentiation in the rat dentate gyrus after intrathecal treatment with adipose-derived mesenchymal stem cells.

Choi, Jung Hoon,Chung, Jin Young,Yoo, Dae Young,Hwang, In Koo,Yoo, Ki-Yeon,Lee, Choong Hyun,Yan, Bing Chun,Ahn, Jin Ok,Youn, Hwa Young,Won, Moo-Ho Kluwer Academic/Plenum Publishers 2011 Cellular and molecular neurobiology Vol.31 No.8

<P>Mesenchymal stem cells (MSC) have emerged as a new therapeutic tool for a number of clinical applications, because they have multipotency and paracrine effects via various factors. In the present study, we investigated the effects of adipose-derived MSC (Ad-MSC) transplantation via intrathecal injection through the cisterna magna on cell proliferation and differentiation of endogenous stem cells in the hippocampal dentate gyrus (DG) using Ki-67 (a marker for proliferating cells), and doublecortin (DCX, a marker for neuroblasts). The transplanted Ad-MSC were detected in the meninges, not in the hippocampal parenchyma. However, the number of Ki-67-immunoreactive cells was significantly increased by 83% in the DG 2 days after single Ad-MSC injection, and by 67% at 23 days after repeated Ad-MSC treatment compared with that in the vehicle-treated group after Ad-MSC transplantation. On the other hand, the number of DCX-immunoreactive cells in the DG was not changed at 2 days after single Ad-MSC injection; however, it was significantly increased by 62% 9 days after single Ad-MSC injection. At 23 days after repeated Ad-MSC application, the number of DCX-immunoreactive cells was much more increased (223% of the vehicle-treated group). At this time point, DCX protein levels were also significantly increased compared with those in the vehicle-treated group. These results suggest that the intrathecal injection of Ad-MSC could enhance endogenous cell proliferation, and the repeated Ad-MSC injection could be more efficient for an enhancement of endogenous cell proliferation and differentiation in the brain.</P>

이용고객에 의한 농산물 도매법인의 평가 : 광주지역 농산물 도매시장의 출하자 및 중도매인을 중심으로 With special emphasis on the producers and intermediary wholesalers of public agricultural products wholesale martek in Gwangju area

안상돈,기회성,김정주 建國大學校 自然科學硏究所 2004 建國自然科學硏究誌 Vol.15 No.-

Since the economic system of capitalism is a society established on the basis of efficiency and competition, the class of people either inefficient or falling behind in competition will gradually weed out. Such principles of the market economy will apply, without exception, to the basic nucleus of the distribution system of the agricultural products, and it is evident that the agricultural products wholesale market companies being managed comparatively inefficiently run the risk of weeding out by themselves. At this point of time when the distribution system of agricultural products becomes diversified and large-sized, and differentiated, the relatively inefficients elements of the agricultural products wholesale market companies may most probably endanger the wholesale market companies, the nucleus of the agricultural products distribution system. The producers and intermediary wholesalers being the main customers of the wholesale market companies, the problems resulting from the conflicts of interest between them are very difficult to compromise and it takes a great deal of efforts and time to satisfy the needs of the producers and the intermediary wholesalers. Nevertheless, it is the hard realities that amid such difficulties, the agricultural products wholesale market companies can not neglect to meet the desires of their main customers, the producers and intermediary wholesalers. Since, however, the wholesale market companies are business entities themselves, it is also evident that they will eventually weed out, if they go against the trend of the times wherein all business entities should pursue "customer emotion" beyond "customer satisfaction". It would, therefore, be very opportune to gauge how the producers and intermediary wholesalers, the two main customers of the wholesale market companies evaluate the use of the wholesale market companies, and how satisfied they are with the services offered by the wholesale market companies. At the same time, such research seems to provide the useful basic materials to establish the survival strategies of the wholesale market companies. The examination of the evaluation, by the producers and intermediary wholesalers, the two main customers of the wholesale market companies, of the use of the wholesale market companies shows that both the producers and the intermediary wholesalers have favorably evaluated the items on (1) improvement of the order of business transactions, (2) rationalization of the level of the market rates such as the commission for sale on consignment, loading and unloading charges and bounties (3) impartiality of auctions, (4) level of satisfaction with reliability and diversity of the information of business transactions, (5) efforts of the employees of the wholesale market companies for collection and sales of the goods and (6) level of satisfaction with the services offered by the employees of the wholesale market companies to main customers. At the same time, when asked whether the use of the wholesale market companies improved their business revenues, the producers (25.0%) and intermediary wholesalers (30.0%) evaluated negatively. Such negative results may very likely lead to threatening factors hampering the growth and development of the wholesale market companies. The above negative results may be even more conspicuous especially under the circumstances where the agricultural products distribution system gets diverse and large-sized and differentiated through revitalization of direct transactions between the producers and large-scale discount marts and the competitions among the companies engaged in the same category of industry are very keen. From the viewpoint of the survival strategy of the wholesale market companies, approaches should be so made as the efforts to improve on the earnings of the producers and wholesale market companies may precede. In the evaluation of the payment methods, most of the producers (90.0%) were affirmative, while intermediary wholesalers were negative (35.0%). However, in consideration of the characteristics of the wholesale market companies having the producers and intermediary wholesalers as their customers, it seems that the methods and procedures for the payments should be improved by all means. In particular, if the wholesale market companies should approach this matter from WIN-WIN strategy, it seems that this matter might be resolved more easily. The changes in the environments faced in management activities of enterprises are quickening and at the same time, the growth or weeding out of enterprises is also quickly determined. Amid such changes, the gauging and satisfying of enterprise desires as the survival strategy must be definitely an extremely important element. The wholesale market company being an business entity itself, should be keen to such changes, and needs to make strategic approaches to its customers, if it does not want to weed out amid such changes.

영어 통제구문에 관한 연구

안종기,서정민 대불대학교 2001 論文集 Vol.7 No.1

The aim of this paper is to discuss the control construction like(1a, b, c) to explain the unexpressed subject of VP[inf]. In traditional framework it depended on Equi-NP deletion or minimal distance principle. In GB, chomsky(1980, 1981) suggested PRO to explain the unexpressed subject of VP[inf]. Manzini(1983) and Kim(1989) revised Chomsky's theory. But none of them can satisfy us to explain the unexpressed subject of Vp[inf]. I solved these problems by using the control theory of HPSG. The unexpressed subject of VP[inf] is token-identical to the INFLUENCED, COMMITTOR, or EXPERIENCER in the psoa, according as the control relation is of influence, commitment, or orientation, resperctively. That is, in(1c) the unexpressed subject of BP [inf] is token-identical to the INFLUENCED(mary), in (1d) the unexpressed subject to VP[inf] is token-identical to COMMITTOR(I) and in (1e) the unexpressed subject of VP[inf] is token-identical to EXPERIENCER(I). Furthermore I applied the control theory of HPSG to VP[inf] within noun phrases.

주정중독 입원환자의 다이설피람요법에 관한 예비연구

안정숙,강봉선,신정호,박기창 大韓神經精神醫學會 1989 신경정신의학 Vol.28 No.3

This study was conducted to investigate the effect of disulfiram on Korean alcoholics, and to evaluate the clinical applicability of disulfiram therapy as an aversive conditioning technique. In twelve alcoholic inpatients, the alcohol challenge test was carried out after loading 500mg/day of disulfiram for 7 days. During the alcohol challenge test, blood was sampled twice, before alcohol ingestion and at the maximal disulfiram-acetaldehyde reaction. Blood-alcohol concentration was measured by enzymatic analysis technique. The severity of aversive reaction was scaled subjectively by the patient. The results are as follows : 1) Side effects of disulfiram were mild and could be considered mostly manifestations of mental or physical predisposition of the alcoholic patient, and disappeared after reducing the dosage. 2) The severity of disulfiram-acetaldehyde reaction was negatively correlated to body weight under given loading dose of disulfiram and given challenging dose of alcohol. 3) Most of the disulfiram-acetaldehyde reaction was self-limited within 2 hours leaving no sequela. 4) Blood-alcohol concentration at the maximal aversive reaction was average 25.3mg/dl(range 21-49mg/dl), and not related to the subjective ratings of the aversive reaction. 5) On the telephone follow-up interview with spouses of the patients, 11 patients were identified, 8 of them(74%) had been abstinent for 3-16 months with only 5 patients on disulfriam maintenance. The other 3 patients had been abstinent for 6-15 months without disulfiram maintenance and regular follow-up after discharge. 6) Therapeutic success of abstinence was significantly correlated with the severity of aversive reaction in alcohol challenge test(p<0.1), but not with the total number of test(p>.05). From this study, we may conclude that disulfiram therapy under medical supervision is not so dangerous as we have concerned and we would suggest that the disulfiram therapy is applicable as an aversive conditioning technique.

A Comparison of Lyophilized Amniotic Membrane with Cryopreserved Amniotic Membrane for the Reconstruction of Rabbit Corneal Epithelium

Ahn Jae-Il,Jang In-Keun,Lee Doo-Hoon,Seo Young-Kwon,Yoon Hee-Hoon,Shin Youn-Ho,Kim Jae-Chan,Song Kye-Yong,Lee Hee-Gu,Yang Eun-Kyung,Kim Ki-Ho,Park Jung-Keung The Korean Society for Biotechnology and Bioengine 2005 Biotechnology and Bioprocess Engineering Vol.10 No.3

Many researchers have employed cryopreserved amniotic membrane (CAM) in the treatment of a severely damaged cornea, using corneal epithelial cells cultured on an amniotic membrane (AM). In this study, two Teflon rings were made for culturing the cells on the LAM and CAM, and were then used to support the AM, which is referred to in this paper as an Ahn's AM supporter. The primary corneal epithelial cells were obtained from the limbus, using an ex-plantation method. The corneal epithelium could be reconstructed by culturing the third­passage corneal epithelial cells on the AM. A lyophilized amniotic membrane (LAM) has a higher rate of graft take, a longer shelf life, is easier to store, and safer, due to gamma irradiation, than a (AM. The corneal epithelium reconstructed on the LAM and (AM, supported by the two­Teflon rings, was similar to normal corneal epithelium. However, the advantages of the LAM over that of the (AM make the former more useful. The reconstruction model of the corneal epithelium, using AM, is considered as a good in vitro model for transplantation of cornel epithelium into patients with a severely damaged cornea.

Reconstruction of Rabbit Corneal Epithelium on Lyophilized Amniotic Membrane Using the Tilting Dynamic Culture Method

Ahn, Jae-Il,Lee, Doo-Hoon,Ryu, Yang-Hwan,Jang, In-Keun,Yoon, Mun-Young,Shin, Youn Ho,Seo, Young-Kwon,Yoon, Hee-Hoon,Kim, Jae-Chan,Song, Kye-Yong,Yang, Eun-Kyung,Kim, Ki-Ho,Park, Jung-Keug Blackwell Publishing Inc 2007 Artificial Organs Vol.31 No.9

<P>Abstract: </P><P>Rabbit corneal epithelium was reconstructed using tilting dynamic culture with a self-manufactured, amniotic membrane (AM) supporter and a lyophilized amniotic membrane (LAM). Rabbit corneal epithelial (RCE) cells were cultured and cryopreserved after isolation from the limbus. The second- and third-passage RCE cells were plated onto the epithelial side of the LAM of Ahn's AM supporter. Two days later, the air–liquid interface culture was maintained with third-passage RCE cells for 6 days and second-passage corneal epithelial cells for 9 days. The average viability of thawed RCE cells, assessed using trypan blue dye exclusion, was 77.42%. The reconstructed corneal epithelium was characterized by histological (hematoxylin and eosin) and immunohistochemical staining (proliferating cell nuclear antigen) for light microscopy, and by reverse transcriptase-polymerase chain reaction, glucose assay, and transmission electron microscopy. The basal layer of the reconstructed corneal epithelium was well formed, and the epithelium was tightly constructed due to the increase in cell proliferation and differentiation caused by the tilting dynamic culture, as opposed to static culture. Tilting dynamic culture was useful for the reconstruction of the epithelium using easily damaged epithelial cells and resulted in more stratum cell layers. Moreover, cytokeratin (CK3) mRNA expression in tilting dynamic cultured third-passage RCE cells seeded onto AM was greater than in static cultured third-passage RCE cells. The morphology of the reconstructed corneal epithelium on LAM by tilting dynamic culture for 9 days resembled that of the skin epidermis. This was thought to be because the tilting dynamic culture not only accelerated the proliferation and differentiation of cells by physical or mechanical stimulation, but also ensured that the supply of medium was delivered to the basal cells more efficiently. Thus, the reconstruction of the corneal epithelium using LAM and tilting dynamic culture was considered to be a good in vitro model for autologous or allogeneic transplantation of corneal epithelium and skin epidermis in patients with damaged epithelia. </P>

하수 2차 처리수 재이용을 위한 저압 및 중압 고도산화시스템의 성능평가

안규홍,안석,맹승규,김기팔,홍준석,정민우,권지향,Ahmed, Zubair 대한상하수도학회 2003 상하수도학회지 Vol.17 No.4

In the present study, the feasibility of UV/H₂O₂ systems was investigated using low and medium-pres sure lamps on biologically treated wastewater effluents for secondary effluent reclamation. Two types of UV lamps were used as the light sources (a 33W lowpressure mercury lamp and a 350-W mediumpressure mercury lamp). The results from these UV systems showed that the removal of organic compounds could be achieved in the contact time of longer than 30min (i.e., low UV doses). Efficiencies of color removal and disinfection were far better than those of organic matters measured as TOC, DOC and TCOD_(cr). In the low pressure lamp UV system, it has been found that DOC and color removals were 60.9 and 86.2% with 50㎎/L of H₂O₂ and contact times of 30 minute, respectively. Whereas, with the mediumpressure lamp UV system, TOC, DOC and color removal were 27.1, 5.6 and 95% with 14.3㎎/L of H₂O₂ and 14 minute of contact times, respectively. Both systems could be applied for the reclamation of secondary effluent treated with biological treatment processes.

석유화학공업 종사자의 유전독성 위험성 평가

안기원,박정선,서순팔,문재동,조진형 大韓産業醫學會 1998 대한직업환경의학회지 Vol.10 No.1

In order to evaluate the genotoxic hazard among workers potentially exposed to low level petrochemical substances, the analyses of micronuclei(MN) and sister chromatid exchanges(SCEs) in lymphocytes were performed in 46 male workers(as exposed group) and 46 nonexposed subjects(as control group). Mean frequencies of MN and SCEs(respectively, 12.9/1000 cells and 6.5/cell) in exposed group were very significantly higher than those(10.2/1000 cells and 5.4/cell) in control group. And there were also significant differences in mean frequencies of MN and SCEs adjusted for age, employment duration, smoking, and drinking between two groups. Median frequencies of MN and SCEs in exposed group were very significantly higher than those in control group. Frequencies of SCEs were higher in smokers than in non-smoker. Frequencies of MN in smokers, however, were similiar to those of non-smoker. Interaction between exposure and smoking on MN and SCEs induction was not observed. The results suggest that there is genotoxic hazard in high risk group like workers handling carcinogens in petrochemical plants and the analyses of MN and SCEs are useful biomarkers for the exposure to hazard substances even at the level below the exposure limit.

자기장 저속 냉동보관법을 이용한 쥐 치아 치주인대세포의 활성도 검사

안현정,김의성,김진,김덕원,김기열,이찬영,이승종 大韓齒科保存學會 2008 Restorative Dentistry & Endodontics Vol.33 No.4

본 연구의 목적은 흰쥐 상악 대구치를 발거하여 자기장 저속 냉동보관법을 이용하여 냉동 시 치주인대세포의 활성도 및 세포 사멸도를 MTT검색법과 TUNEL검사를 이용하여 측정하고자 하였다. 4주령의 암컷 Sprague-Dawley계 된 쥐의 상악 좌우 제1,2대 구치를 발거하여 각군 당 12개의 쥐 치아를MTT검색에 이용하였고 6개의 치아를 TUNEL 검사에 이용하였다. 실험군은 5개군으로 대조군은 즉시 발치군이며 4℃냉장고에서 1주일간 보관한 냉장군, 발치 후 동해방지제 처리과정을 거쳐 -196℃의 액화질소에 넣어 급속 냉동한 액화질소군, 21.7 ㎃, 60 ㎐, 1 G의 자기장을 이용하여 -0.3℃/min 의 속도로 -20℃까지 냉동 후 -196℃로 급속 냉동한 자기장군, -0.3℃/min의 속도로 -20℃까지 냉동 후 -196℃에 급속 냉동한 저속 냉동군으로 나누었다. 보존액은 F medium을 사용했으며 동해방지제로 10% dimethyl sulfoxide (DMSO)를 사용하였다. 치근면을 단위면적으로 표준화하기 위해 MTT측정값을 Eosin 염색 후 530 ㎜에서 측정한 흡광도 값으로 나누었다. TUNEL 검사 시 각 조직슬라이드에서 400배 크기의 현미경 시야에서 임의로 세 부분을 지정하여 정상 세포수와 양성 세포수를 세어 그 비율을 계산하여 각 실험군 당 평균치를 구하였다. 통계 분석을 위해 one way ANOVA를 시행하였으며 사후검정으로 Scheffe와 Tukey HSD방법을 썼으며 결과는 다음과 같다. MTT검색에 의한 흡광도를 Eosin염색 후 측정한 흡광도로 나눈 값에서는 자기장군은 즉시 발치군보다 낮은 세포활성을 보였고 (p < 0.05) 액화질소군, 저속 냉동군과는 통계적으로 유의성 있는 차이를 보이지 않았다. 그러나 자기장군은 액화질소군, 저속 냉동군과 함께 냉장군보다는 높은 세포 활성도를 보였다 (p<0.05).TUNEL검사 결과도 자기장군은 즉시 발치군보다 치주인대의 세포사멸도가 높았으나 (p < 0.05) 저속 냉동군과 액화 질소군과는 통계적으로 유의한 차이를 보이지 않았다. 자기장군은 냉장군보다 세포사멸도가 낮았으며 냉장군은 모든 군 중에서 세포 사멸도가 가장 높았다 (p<0.05). The purpose of this study was to evaluate the viability of periodontal ligament cell in rat teeth using slow cryopreservation method with magnetic field through MTT assay and TUNEL test. For each group, 12 teeth of 4 weeks old white female Sprague-Dawley rat were used for MTT assay, and 6 teeth in TUNEL test. The Maxillary left and right, first and second molars were extracted as atraumatically as possible under tiletamine anesthesia. The experimental groups were groupl (immediately extraction), group 2 (cold preservation at 4℃ for 1 week), group 3 (rapid Cryopreservation in liquid nitrogen), group 4 (slow cryopreservation with magnetic field of 1 G), and group 5 (slow cryopreservation). F medium was used as preservation medium and 10% DMSO as cryoprotectant. After preservation and thawing, the MTT assay and TUNEL test were processed. One way ANOVA and Scheffe method were performed at the 95 % level of confidence. The value of optical density obtained after MTT analysis was divided by the value of eosin staining for tissue volume standardization. In both MTT assay and TUNEL test, it had showed no significant difference among group 3,4, and 5. And group 3 had showed higher viability of periodontal ligament cell than group 2. From this study, slow cryopreservation method with magnetic field can be used as one of cryopreservation methods.