Antimutagenic Effects of Ginsenoside Rb$_1$, Rg$_1$ in the CHO-K1 Cells by Benzo[a]pyrene with Chromosomal Aberration Test and Comet Assay

Kim, Jong-Kyu,Kim, Soo-Jin,Rim, Kyung-Taek,Cho, Hae-Won,Kim, Hyeon-Yeong,Yang, Jeong-Sun The Korean Society of Toxicogenomics and Toxicopro 2009 Molecular & cellular toxicology Vol.5 No.2

The usage and types of chemicals are advancing, specializing, large-scaled increasing, and new chemical exposed workers are concerning to occupational disease. The generation of reactive oxygen in the body from carcinogen, mutation and DNA damage in cancer is protected by natural antioxidants (phytochemicals) with antimutagenic effect. There were many reports of ginsenoside Rb$_1$, Rg$_1$ grievances of the genetic mutation to suppress the effect confirm the genetic toxicity test with chromosomal aberration test and the Comet (SCGE) assay confirmed the suppression effect occurring chromosomal DNA damage. We had wanted to evaluate the compatibility and sensitivity between the chromosomal aberration (CA) test and the Comet assay. We used the CA test and Comet assay to evaluate the anti-genotoxicity of ginsenoside Rb$_1$ and Rg$_1$, in CHO-K1 (Chinese hamster ovary fibroblast) cell in vitro, composed negative control (solvent), positive control (benzo[a]pyrene), test group (carcinogen+variety concentration of ginsenoside) group. The positive control was benzo[a]pyrene (50 $\mu$M), well-known carcinogen, and the negative control was the 1 % DMSO solvent. The test group was a variety concentration of ginsenoside Rb$_1$, Rg$_1$ with 10$^{-8}$%, 10$^{-6}$%, 10$^{-4}$%, 10$^{-2}$%, 1%, 10%. In chromo-somal aberration test, we measured the number of cells with abnormally structured chromosome. In Comet assay, the Olive tail moment (OTM) and Tail length (TL) values were measured. The ratio of cell proliferation was increased 8.3% in 10$^{-8}$%, 10$^{-6}$%, 10$^{-4}$%, 10$^{-2}$%, 1%, 10% Rb$_1$ treated groups, and increased 10.4% in 10$^{-10}$%, 10$^{-8}$%, 10$^{-6}$%, 10$^{-4}$%, 10$^{-2}$%, 1% Rg$_1$ treated groups. In the CA test, the number of chromosomal aberration was decreased all the Rb$_1$ and Rg$_1$ treated groups. In the Comet assay, the OTM values were decreased in all the Rb$_1$ and Rg$_1$ treated groups. To evaluate the compatibility between CA and Comet assay, we compared the reducing ratio of chromosomal abnormalities with its OTM values, it was identified the antimutagenicity of ginsenoside, but it was more sensitive the CA test than the Comet assay. Ginsenoside Rb$_1$ and Rg$_1$ significantly decrease the number of cells with chromosomal aberration, and decrease the extent of DNA migration. Therefore, ginsenoside Rb$_1$, Rg$_1$ are thought as an antioxidant phytochemicals to protect mutagenicity. The in vitro Comet assay seems to be less sensitive than the in vitro chromosomal aberration test.

虹彩 體質 分析에서 心腎結合組織의 虛弱 體質과 高血壓 家族歷과 관련성 硏究

金鐘牛,黃祐準,琴坰樹,李始炯,李宗淳,都金錄,趙在運,趙州掌,金鐘煜 한국전통의학연구소 2002 한국전통의학지 Vol.12 No.1

Iridology, developed more than 100 years ago, is the diagnosis of medical conditions through noting irregularities of the pigmentation in the iris The purpose of this study is to compare interrelation between CVA, hypertension family history and cardio-renal connective tissue in Iris Constitution through iris constitution examination. The subjects consist of 114 patients who were examined in Iris Constitution at Chonju Oriental Medicine Hospital attached to Wonkwang Univ. from September. 1St. 2000 to August. 31th. 2002. 1. In the distribution of Iris Constitution, among of 79cases, There are neurogenic type 13cases, abdominal connective tissue weakness constitution 31 cases, cardio-renal connective tissue weakness constitution 6 cases, cholesterol 7 cases and others 22 case in control group. 2. There are familial history of CVA and hypertention 32 cases, no familial history of CVA and hypertention1 3 cases, in 35cases of experimental group. These results imply that there is meaningfulness of interrelation between cardio-renal connective tissue weakness constitution and. CVA, hypertention.

老齡者의 建康을 위한 生活習慣 實態調査

申東敏,長鳳愚,李揆文,金賢俊,金昌範,崔宗洙,崔鍾晥,李鍾珏,金圭碩,趙庚旭,金鍾聲,朴鍾振 平生體育硏究所 論文集 1987 平生體育硏究所 論文集 Vol.2 No.-

With a view to examining the habit and method in which old people maintain and improve their health, we consulted 551 men and 444 women who were over 65 years old, and concluded as follows: 1. Habit of eating It's been revealed that most of the old people keep a regular eating hour, control the quantity of meal or eat a little food, and prefer vegetables to meat. 2. Mental health Old people feel the necessity for mental health and most of them (about 80%) keep good mental health without becoming pessimistic. Their chief concenrs have turned out to be about their sons and daughters and their own health and finance. 3. Managing Good Health It has been revelaed that old people do not possess any special kind of secret to keep good health but taking plenty of rest, regular meal, and proper exercise, and many of them (about 60%) have been shown to eat invigorant food that they can easily get around their living area. 4. Physical Functions It has been revealed that many old people are conscious of some symptoms about their parts of body and about to percent of them suffers from neuralgia. Some measures like medical aid especially for old people need to be taken as soon as possible. As we have found that the health-keeping method of old people consists plenty of rest, proper exercise, and appropriate nutrition, we can conclude that the recreatonal and culture facilities and programs for keeping old people in good health should be developed. It is also necessary to study food that contribute to their health and develop ways to get rid of the stresses to which they are exposed in their daily life. Presides, it is natural that government found an administrative department to deal with these matters effectively.

폴리에스테르 섬유 감량시스템용 프로세서 타이트레이터 개발

조진호,이흥락,이종현,김명남,구성모,김무영,진경찬,유병희,강민수,김국진 경북대학교 센서기술연구소 1993 연차보고서 Vol.1993 No.-

폴리에스테르 섬유 감량시스템의 제어를 위해서는 처리조 내에서 감량되는 섬유가 목표 감량치에 도달했는지를 판단해 주는 장치가 필요하다. 본 연구에서는 가성소다용액의 초농도 및 섬유 감량 정도의 정확한 측정과 감량 종료시간의 산출 및 이에 따른 종료신호를 발생할 수 있는 폴리에스테르 섬유 감량기 전용의 자동적정장치를 개발하였다. To control caustic soda treatment system for the polyester fabric reduction, we need the device to determine that the fabric has been arrived target reduction value. In this study, we develope an automatic process titrator for the caustic soda treatment system. Major functions of the target titrating system are accurate measurements of the caustic soda concentration and then producing the end point signal for caustic soda treatment system.

한우에 있어 황토 첨가급여가 면역기능에 미치는 영향

조원모,기광석,정하연,강수원,김준식,오성종,김용국 한국국제농업개발학회 2003 韓國國際農業開發學會誌 Vol.15 No.2

본 연구는 한우 육성우에 대한 황토 첨가급여가 항병성에 미치는 효과를 구명하고자 혈액성상변화를 규명한바 아래와 같은 결과를 얻었다. 대조구 및 처리구에서 전체적인 혈액성상의 변화는 없었으나 백혈구수치가 대조구 및 처리구에서 각각 15.35K/㎕ 및 13.37K/㎕이였으며, 적혈구에 있어서도 정상수치인 5~10M/㎕보다 대조구 10.03M/㎕, 처리구 11.35M/㎕로 모두 높은 수치를 보였다. 황토첨가에 따른 혈액성상의 변화는 처리간에 유의한 차이는 보이지 않았다. The study was conducted to investigate the effect of dietary supplements of reddish clay on the immunity of growing Hanwoo steers. A total of 8 calves were divided into 2 feeding groups, control(basal diet) and treatment(basal diet plus ad libitu reddish clay) for Hanwoo steers. This study was carried out for 5 months using 8 calves. The results obtained by the present study are summarized as follows : The proportion of WBC, NE as well as LY(15.35, 6.85, 6.86K/??) was higher in the control group than in the Reddish clay-supplemented group. But no statistical differences were found in blood substances among treatment calves.

금융시스템에서 원격전송 오류의 자동검출 방법

조수종,손유익 啓明大學校 産業技術硏究所 1998 産業技術硏究所 論文報告集 Vol.21 No.2

As the bulk of information among banking systems is increased, it is highly possible to occur the loss of data due to lots of reasons In this paper automatic error detection algorithms are presented for upgrading the reliability of banking systems. In this design two diagnostic software systems are developed for solving the problem automatically. One is for the session errors and the other for the communication link errors The proposed fault-tolerance diagnostic systems offer high fault-tolerance; error rate and processing rate. As a result, we have shown that the proposed method has a high probability of survival.

FET형 포도당 센서를 위한 PVP/DAST/GOD 고정화막 형성기술

조병욱,이수근,박이순,이종덕,손병기 경북대학교 센서기술연구소 1992 센서技術學術大會論文集 Vol.3 No.1

21세기를 향한 국가기술자격제도의 발전방안 연구 : New Directions Towards the 21st Century

조정윤,박종성,김현수 한국산업교육학회 1999 산업교육연구 Vol.6 No.-

21세기를 대비한 국가기술자격제도의 발전 방안을 국가 경쟁력 확보 측면에서 모색하기 위해 이 연구에서는 국제 경제 환경 변화에 따른 주요국의 자책제도 대응 방향을 살펴봄으로써 우리나라 국가기술자격제도에 반영해야 할 시사점을 도출하였다. 이와 함께 국가기술자격제도의 운영 실태를 파악하고 자격제도에 관한 요구를 파악하기 위해서 39개 기관을 방문하여 넓은 의미의 국가기술자격 관리자들을 대상으로 면담조사를 수행하였고, 기 국가기술자격 취득자 480명을 대상으로 설문조사를 실시하여 의견을 수렴하였다. 이상의 연구를 통해 21세기를 향한 국가기술자격제도의 발전 방향을 기술·기능 분야의 합리화 방안, 서비스 분야의 합리화 방안, 자격 취득자의 사회적 우대 방안, 자격 정보체제구축 방안, 기초직업능력과의 연계 방안, 민간자격으로 관리·운영의 전환 방안, 국제적 통용성 확보 방안, 통합 국가자격체제 구축 방안으로 나누어서 제시하였다. There are strong links between a nation's economic prosperity, its international competitiveness, and the competency of its population. As international competition intensifies and the rate of economic and technological change accelerates, advanced countries increasingly strive to improve their competitiveness by reforming their national qualification systems. With the advent of the information age and the knowledge- based society, human resource development has become a key factor in determining a nation's competitiveness. And technological qualificatior systems have a direct and significant influence on human resource development. In order to guarantee Korea's continued development as a competitive member of the international community, it is demanded that a Korean Technology Qualification System(KTQS) be established to set Ae nation's future course for the 21st century. Extensive and concrete development plans for KTQS in 21st centry are as follows: First of all, it is essential tc prepare policies for improving the effectiveness and suitability of KTQS and its operations as they apply to the domains of technology and craft. Second, in terms of the service fields, policy measures should be taken to improve and guarantee the diversity of qualification items. Third, appropriate policy measures should be taken in order to raise the general social status of NTQ holders. Fourth, policies should be introduced which will allow for the enhancement of generic competency under the qualification system. Fifth, appropriate policies should be prepared to facilitate a partial transition from a state-run qualification system to a private system. Sixth, with the recent development of international agreements on profession qualifications, it is important to guarantee that Korean qualifications have a common, international currency. Finally, it is imperative that policies be prepared which can establish the necessary foundations for the unification of national qualifications under a single NQ.

Polytrop Blue B 및 hydroxydodecyl-trimethyl ammonium bromide에 依한 微量銅의 吸光度定量에 關한 硏究

趙誠烋,金永洙,鄭鐘南 朝鮮大學校 1977 綜合論文集 Vol.1977 No.-

We Can notice reaction of PolytropBlue B with Cu in the existance of hydroxydodecyl trimethyl. Ammonium bromide to make a blue ion-assossiation chelate. The chelate turns out stable in aquesous pyridine solution maximum absoption of this was measured at 591nm, and the absorbance is Constant from, pH, 8.0 to 9.2. When molar extintion Coefficient is 1.19×10??, very high Sensitivity was noticed. This Value is 8 times higher than that of batho-cupronin chelate. Considerable interference was detected from Co, Ni, Zn, U, Ce, Cr, Fe, and Sn. From over 0.2㎍ Cu/ml the absorbance follows Beer's Law.

변단면 고층건물의 변동풍압특성

조지은,김동환,김종수,김영문 대한건축학회 2003 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.23 No.2(구조계)

Nowadays the assessment of structural safety and serviceability of a tall building during strong wind is one of the important design criteria. The purpose of this study is to predict the pressure fluctuation of the tall building have different taper ratio of 5%, 10%, 15%. According to taper ratio, to know that the effect of vibration control on a tapered tall building attacked across wind. And also to give basic concepts of the tapered building on unpredictable wind directions. Acquired pressure data allowed analysis of the mean and r.m.s pressure coefficient, power spectral density function in measured taps at different levels and columns with considering the effects of wind directions and boundary layer conditions.