Heme oxygenase-1 induced by desoxo-narchinol-A attenuated the severity of acute pancreatitis via blockade of neutrophil infiltration

Bae, Gi-Sang,Kim, Dong-Goo,Jo, Il-Joo,Choi, Sun-Bok,Kim, Myoung-Jin,Shin, Joon Yeon,Kim, Dong-Uk,Song, Ho-Joon,Joo, Myungsoo,Park, Sung-Joo ELSEVIER 2019 INTERNATIONAL IMMUNOPHARMACOLOGY Vol.69 No.-

<P><B>Abstract</B></P> <P>Heme oxygenase-1 (HO-1) has an anti-inflammatory action in acute pancreatitis (AP). However, its mechanism of action and natural compounds/drugs to induce HO-1 in pancreas are not well understood. In this study, we investigated the regulatory mechanisms of HO-1 during AP using desoxo-narchinol-A (DN), the natural compound inducing HO-1 in the pancreas. Female C57/BL6 Mice were intraperitoneally injected with supramaximal concentrations of cerulein (50 μg/kg) hourly for 6 h to induce AP. DMSO or DN was administered intraperitoneally, then mice were sacrificed 6 h after the final cerulein injection. Administration of DN increased pancreatic HO-1 expression through activation of activating protein-1, mediated by mitogen-activated protein kinases. Furthermore, DN treatment reduced the pancreatic weight-to-body weight ratio as well as production of digestive enzymes and pro-inflammatory cytokines. Inhibition of HO-1 by tin protoporphyrin IX abolished the protective effects of DN on pancreatic damage. Additionally, DN treatment inhibited neutrophil infiltration into the pancreas via regulation of chemokine (C-X-C motif) ligand 2 (CXCL2) by HO-1. Our results suggest that DN is an effective inducer of HO-1 in the pancreas, and that HO-1 regulates neutrophil infiltration in AP via CXCL2 inhibition.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Desoxo-narchinol-A (DN) is a natural compound of HO-1 inducer in pancreas. </LI> <LI> Mechanism of DN-induced HO-1 is mediated by MAPK/Activator Protein-1/HO-1 signaling. </LI> <LI> DN-induced HO-1 blocks neutrophil infiltration into pancreas via inhibition of CXCL2. </LI> <LI> DN inhibits cerulein-induced acute pancreatitis (AP) and AP-associated lung injury. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

2차병원에서의 소아과 환자의 응급실 및 외래 이용에 관한 연구

김진희,배진호,전진호,문정웅 인제대학교 2006 仁濟醫學 Vol.27 No.-

Objectives : To analyze the characteristics of the pediatric patients who visited the emergency room and outpatient department of secondary hospital in order to properly guide pediatric patients to use emergency room and raise the efficiency Methods : The authors investigated and analyzed sex, age, visit day, visit time, length of stay, hospitalization and diagnostic entities of the pediatric patients who visited the emergency room and outpatient department of Wallace Memorial Baptist Hospital from January 2003 to December 2005 in a retroactive approach with their medical records. Results : For the three years, the number of total patients who visited the emergency room was 56,051 and among them, the number of pediatric patients was 7,490 (13.4%). The age group of 1 to 2 years was the largest with 2,488 (33.2%). The male to female ratio was 1.34:1. Sunday was the day of the week that the largest number of patienst visited with 2,375 (31.7%) and nearly half (48.4%) of the patients visited the emergency room during the weekend. The time when the most patient visited the emergency room was between 8 pm to 12 am. Regarding the length of stay, the highest number of patients (65.0%) stayed in the emergency room for on hour or less, and the average length of visit was 1.5 hours, which was rather short. Most patients (68.8%) left the hospital after taking simple physical examination and procedure(prescription). The most frequent diagnoses for the pediatric patients who visited the emergency room were acute pharyngitis (26.0%), acute gastroenteritis (20.7%), fever (6.6%). During the same period, the total number of pediatric patients who visited the outpatient department was 125,513. The age group of 3 to 5 was the largest with 36,041 (28.7%). The male to female ratio was 1.34:1. 6.0% of total outpatients required treatment in a hospital. Most frequent diagnoses of the outpatients were asthma (24.4%), pneumonia (12.0%), vaccination (8.7%), febrile convulsion (8.4%), acute pharyngitis (6.6%), acute gastroenteritis (5.8%). Conclusion : It is considered that a large number of pediatric patients still have used emergency room for treatment of simple and mild diseases instead of visiting outpatient department and/or primary hospital. Thus further efforts are required to properly guide pediatric patients to use emergency room and to raise the efficiency of their use.

한국 정신장애의 역학 조사 연구[I] : 각 정신장애의 유병률

조맹제,함봉진,김장규,박강규,정은기,서동우,김선욱,조성진,이준영,홍진표,최용성,박종익,이동우,이기철,배재남,신정호,정인원,박종한,배안,이충경 大韓神經精神醫學會 2004 신경정신의학 Vol.43 No.4

Objectives : This study aims to estimate the prevalence of the DSM-IV psychiatric disorders in Korean population using the Korean version of Composite International Diagnostic Interview (K-CIDI). Methods : Subjects were selected by taking multi-stage, cluster samples of 7,867 adult household residents, 18 to 64 years of age, in ten catchment areas. Total 78 trained interviewers administered the K-CIDI to the selected respondents, from June 1 to November30,2001. Results : Total 6,275 respondents completed the interview. Some 33.5% of respondents reported at least one lifetime disorder, 20.6% reported at least one-year disorder, and 16.7% reported at least one-month disorder. The most common lifetime disorders were alcohol abuse/dependence (17.24%), nicotine dependence/withdrawal (11.19%), specific phobia (5.16%), and major de-pressive disorder (4.25%). The lifetime prevalence of substance abuse/dependence (0.25%) and schizophrenia (0.16%) was very low. Nicotine and alcohol use disorder showed very high male/female ratio. Mood disorder and anxiety disorder were more prevalent among female than male. Conclusion : The prevalence of psychiatric disorders was high. In comparison with other studies, remarkable differences in distributions of psychiatric disorders across the areas and times were observed.

신규 방사성 항암제 DW-166HC 의 소핵시험

문은이(Eun Yi Moon),이진(Jin Lee),이원용(Won Yong Lee),최청하(Chung Ha Choi),이덕근(Dug Keun Lee),유제만(Jei Man Ryu),정용호(Yong Ho Chung),윤성준(Sung June Yoon),박경배(Kyung Bae Park) 한국응용약물학회 1997 Biomolecules & Therapeutics(구 응용약물학회지) Vol.5 No.3

DW-166HC (^(166)Holmium (^(166)Ho)-Chitosan complex) is a new radiopharmaceutic anticancer agent with a broad anti-tumorigenic spectrum, especially against human hepatic cancer. DW-166HC was evaluated for the appearance of micronucleus in polychromatic erythrocytes (PCEs) of mouse bone marrow cells after subcutaneous arid intravenous single administration. Bone marrow cells were prepared at 24 hr and 48 hr after DW-166HC-I (^(165)Ho-Chitosan complex : cold compound) administration and at 24 hr, 72 hr and 2 weeks after DW-166HC (^(166)Ho-Chitosan complex : hot compound) administration. The results showed there was no statistically significant increase of the numbers of PCEs with micronucleus in all DW-166HC-I administered groups compared with a negative control group but there was statistically significant increase of the numbers of PCEs with micronucleus at 24 hr arid 72 hr in all DW-166HC administered groups, which was recovered after 2 weeks from the drug administration. The results also showed the ratio of normochromatic erythrocytes (NCEs) to PCEs of all DW-166HC-I administered groups was not significantly different from that of a negative controi group but there was significant difference of this ratio at 24hr and 72 hr in all DW-166HC administered groups compared with that of negative group, which was also recovered after two weeks from the drug administration. These results suggested that DW-166HC-I may not cause any chromosomal damage but DW-166HC has in vivo mutagenic potential because of its radioactivity.

아황산가스가 흰쥐 허파조직내 Laminin 활서에 미치는 영향에 대한 면역조직학적 연구

배성만,정호삼,서윤경,백두진,김원규,윤지희 한양대학교 의과대학 2000 한양의대 학술지 Vol.20 No.2

Sulfur dioxide (SO_2), a kind of air pollutant, causes harmful damage to human body. In particular, inhalation of sulfoxide dioxide has been demonstrated to result in the injury to the upper respiratory duct. However, mechanisms by which SO_2 affects these tissues remain to be clarified. In the present study, to investigate the mechanism of SO_2 effects, the influence of SO_2 exposure was examined in terms of the injury of lung and the expression pattern of laminin in the basal lamina. The basal lamina is an important tissue for the regulation of internal respiration by composing of air-blood barrier. Sprague-Dawley rats repetitively exposed to a mixture of O_2 gas and SO_2 gas (250 ppm) for 30 minutes a day were sacrificed to observe the distribution of laminin in the alveolar septum as well as the morphological alteration of alveoli using immunohistological methods. The resutls we observed were as follows: 1. Alveoli from SO_2-exposed rats (for 5 days) were strongly stained with anti-laminin antibody, suggesting laminin expressed at the high level, at week 1 to 3 upon exposure to SO_2 2. At week 1 to 3 after exposure of rats to SO_2, alveolar septa were collapsed, leading to the reduction of alveolar volume along with morphological changes to irregular shapes. 3. At week 4 to 6 after exposure of rats to SO_2, alveoli were weakly stained with anti-laminin antibody, suggesting laminin expression was decreased during this period. 4. Rats sacrificed at week 7 upon exposure to SO_2 exhibited the expansion of new alveoli and the expression of laminin was partially recovered up to the intermediate level. Taken together, these results demonstrate that the expression of laminin was enhanced in the early phase, followed by downregulation in the late phase. Moreover, lung injury and resolution were correlated with the level of laminin. Thus, these results suggest that SO_2 suppresses the expression of laminin, which may be associated with the neo-generation of lung tissue.

무리말뚝의 수평하중분담에 관한 실험적 연구

裵鍾淳,金成浩,崔震宇,姜秉卓 경상대학교 생산기술연구소 2000 生産技術硏究所論文集 Vol.16 No.-

Load distribution in group piles is an important matter of consequence for the safe design and the economical design. In the group piles, the model tests were to investigate the effects on spacing-to-diameter ratio of pile, number of piles and arrangement of the pile. The tops of pile were all set to rotate freely. This paper considered the load distribution by the location of the pile as compared the behavior of group piles with single pile. For a three row pile group at 3D spacing, the measured individual row distributions were 35~38%(lead), 32∼34%(middle) and 27∼30%(tail). This results were compared very favorably to those by McVay et al(1995). The shadowing effect for the parallel direction of lateral loading appears to be more significant than the one for the perpendicular direction of lateral loading.

C6 glia 세포에서 유도성 Nitric Oxide Synthase 유전자 발현조절에 관한 연구

배진영,허강민,배소현,박지선,이충재,이재흔,석정호 충남대학교 의과대학 의학연구소 2003 충남의대잡지 Vol.30 No.1

To investigate transcriptional regulation of iNOS gene by LPS and cytokines, the production of NO, expression of iNOS mRNA and protein, binding activity of nuclear factor-kappa B(NF-kB), and promoter activity of iNOS gene were examined in rat C6 glial cells. LPS, interferon-gamma(IFN-γ), and tumor necrosis factor-alpha (TNF-α) stimulated the production of NO, which was increased synergistically by co-treatment. By the treatment of LPS, iNOS mRNA expression was initiated at 1 h, markedly increased by 3 h, and decreased gradually afterward. iNOS mRNA expression was markedly enhanced by mixture of LPS, IFN-γ and TNF-α. iNOS protein synthesis was increased by the treatment of mixture LPS and cytokine mixture. Treatment of LPS stimulated NF-kB activation, and the activation reached to the maximum level at 30 min, and the treatment of mixture of LPS and cytokines increased the activation. To determine the effect of NF-kB binding activity on iNOS promoter activation, CAT assay was performed. iNOS promoter activity was increased by the treatment with LPS for 5.5 h, and further increased by the combined treatment with LPS and cytokines. These results suggest that NF-kB activation by LPS and cytokines may play a significant role in the induction of the iNOS gene.

다량의 균일한 액적 제조를 위한 분리막 기반 미세유체 소자 시스템

장문배, 유진오, 염경호 충북대학교 산업과학기술연구소 2013 산업과학기술연구 논문집 Vol.27 No.2

In this study, we designed the microfluidic device system based on the separation membrane for generating monodisperse emulsion droplets with diameters ranging from hundreds of nanometers to several micrometers. To generate fine droplets, T-junction and flow-focusing geometry are integrated into the microfluidic channel. Also put the membrane into the microfluidic device to make droplets effectively. PDMS devices can be easily, quickly, and inexpensively fabricated with superb resolution. However, PDMS devices are swelled other organic solvent. So we could produce droplets using fluorocarbon oil. The droplet size was controlled by adjusting the flow ratio between continuous phase flow and dispersed phase flow and also membrane pore size

골격성 Ⅱ급 부정교합자의 악교정 수술 후 설골의 위치와 상기도 변화에 관한 연구

배진성,김경호,박형식,허종기,박광호 大韓顎顔面成形再建外科學會 2001 Maxillofacial Plastic Reconstructive Surgery Vol.23 No.6

OSAS(obstructive sleep apnea syndrome) is becoming more and more of importance nowadays. Therefore the study concerning OSAS is very important. Most of the studies which have been reported were comparison of upper airway size and change of skeletal class Ⅰ group and skeletal class Ⅲ group respectively. But, most of the showing OSAS have pharyngeal narrowing combined with more or less distinct maxillary and mandibular deficiency. Therefore the aim of this study is to see posterior airway space and hyoid bone position in patients affected by class Ⅱ malocclusion and treated with orthognathic surgery. we measured the lines between selected upper air way landmarks on lateral cephalometric x-ray films of skeletal class Ⅱ 30 persons who had not been operated yet, were within 2 weeks after operation, were 6 months after operation. And we compared them respectively and analyzed them with pairing t-test, pearson correlation coefficient and multiple regression analysis. The results were as follows. First of all, skeletal class Ⅱ group was increased in all the level of PAS, especially PAS(MK) and PAS related to hyoid bone(H-phW, H-PhW(Me-H)) was significantly decreased within 6 months after operation but that was larger than preoperation. And then the PAS was increased after mandibular advancement surgery. While there was no significant correlation between PAS and surgical methods, there was statistical significance between PAS and a number of surgical movement. At last, the hyoid bone was advanced within 2 weeks after operation but relapsed within the follow up period. In the end of the study, it was turned out that PAS was always wider without distinction of the region after the operation and that there was significant correlation between the moving volume of hard tissues and the changing quantity of PAS( ; how much PAS was changed according to the moving degree of hard tissues). After this, to be based on the study, I consider that OSAS demands further study and that it is necessary to do more practical applications to the patients.

SM45C강의 피로균열 진전 특성과 피로수명 예측

裵原鎬,金鎭烈 慶一大學校 1992 論文集 Vol.8 No.1

Generally, machine shaft which had defect or notch might be cracked by cyclic bending or torsion. The initiated crack at the small defect on the surface of shaft propagates to failure by cyclic load. Fatigue crack growth behavior of SM45C steel was investigated and the crack growth rate was expressed by stress intensity factor range, K. Fatigue life could predicted by the use of stress or strain at the notch tip, and is known that the strain method is more effective than stress method. To estimate the fatigue life, the linear rule was adopted, base line notch factor was used to estimate the strain at notch tip. It was assumed that stress concentration foctor Kt, and strain concentration factor Ke have the same value with the base line notch factor in plain strain condition like a small notch. The estimated fatigud life was compared with expelimental result, and the predicted life was well according with experiments. So that, it was found that the method by base line notch factor is useful to predicted fatigue life of small notched round bar.