Pseudomonas sp. BCNU 171균에서의 유기용매 shock반응과 heat shock반응의 연관성

이승범,김미림,정미연,박정욱,김민정,주우홍 Institute of Genetic Engineering Changwon National 1999 Gene and Protein Vol.3 No.1

The response of Pseudomonas sp. BCNU 171 to various forms of stress was examined by using O'Farrell two-dimensional gel electrophoresis. The toluene resistance of mid-growth cells of Pseudomonas sp. BCNU 171 grown at 30℃ was enhanced by exposing the cells to a prior toluene shock in the presence of 0.05% toluene for 30 min. The heat tolerance of the cell was also induced by the same condition. Individual protein expression was examined at 30 min and 60 min by silver staining after the addition of 0.05%(v/v) toluene to mid-growing cultures of the strain BCNU 171. The toluene stress response increased the expression of eighteen proteins between 30 and 60 min toluene exposure. At 60 min after toluene addition, a toluene specific protein was detected. A few proteins induced by toluene and heat were overlapped on 2D-PAGE.

허혈 유사 조건이 심방근의 활동전위 특성에 미치는 영향

김재하,주정민,조용범,신동호,안병희,주신배,김상형 대한순환기학회 1999 Korean Circulation Journal Vol.29 No.11

복합레진 표면의 연마방법에 따른 표면조도

민정범,조공철,조영곤 대한치과보존학회 2007 Restorative Dentistry & Endodontics Vol.32 No.2

본 연구는 복합레진의 종류와 연마방법, 복합레진 연마 후 레진전색제의 적용이 복합레진의 표면조도에 미치는 영향을 알아보기 위하여 복합레진의 표면조도 측정과 FE-SEM 관찰을 통하여 상호 비교하였다. 미세입자형과 nanofill 복합레진을 이용하여 8×3×2 mm의 시편을 각각 100개씩 제작하였고, 연마방법과 레진전색제의 적용 유무에 따라 20개의 군으로 분류하였다. 복합레진의 종류에 따라 Micronew를 사용한 군은 M군, Filtek Supreme을 사용한 군은 S군으로 분류한 후, 연마방법에 따라 M1군과 S1군은 polyester strip만을 사용한 군, M2군 와 S2군은 Sof-Lex disc를 사용한 군, M3군과 S3군은 Enhance disc와 polishing paste를 사용한 군, M4군과 S4군은 Astropol disc를 사용한 군, M5군과 S5군은 carbide finishing bur를 사용한 군으로 다시 분류하였다. 또한 연마 후 Biscover 레진전색제를 적용한 군은 연마방법에 따른 각 군에 B를 추가하여 M1B군-M5B군 및 S1B군-S5B군으로 분류하였다. 제작된 모든 시편은 표면조도의 측정과 주사전자 현미경 관찰을 시행하기 전에 실온의 증류수에서 24시간 동안 보관 하였다. 표면조도측정기를 사용하여 각 시편의 상면과 하면 모두에서 각각 5회씩 측정하여 평균 표면조도 값을 산출하여 통계적으로 분석하였으며, 각 군에서 대표적인 시편 1개를 선택하여 FE-SEM에서 관찰하여 다음과 같은 결과를 얻었다. 1. 복합레진의 표면조도 값은 polyester strip에 의해 형성된 모든 군에서 가장 낮게 나타났고, 레진전색제를 적용한 S군을 제외한 다른 모든 군에서 finishing bur로 연마한 군이 가장 높게 나타났다 (p < 0.05). FE-SEM 관찰에서 레진전색제를 적용하기 전의 표면은 polyester strip을 사용한 M1군과 S1군이 가장 평활한 표면을 나타냈고, finishing bur를 사용한 M5군과 S5군은 필러의 탈락에 의해 형성된 여러 개의 기포와 함께 울퉁불퉁한 표면을 보였다. 2. 복합레진의 종류에 따른 표면조도는 레진전색제를 적용하기 전에는 통계학적으로 차이를 나타내지 않았으나, 레진전색제를 적용한 후에는 Micronew가 Filtek Supreme보다 평활한 표면을 나타내었다 (p < 0.05). 3. 레진전색제를 적용하기 전에는 복합레진의 종류와 연마방법에 따른 표면조도 간에 상호 연관성이 있었으나, 레진전색제를 적용한 후에는 복합레진의 종류와 연마방법 간에 상호 연관성이 없었다. FE-SEM 관찰에서 레진전색제를 적용한 후의 표면은 모든 군에서 대체적으로 평활한 표면을 나타내었다. 4. 동일한 복합레진과 연마방법으로 처리된 군에서 레진전색제 적용 전과 후의 표면조도 값은 M1B군이 M1군보다, S1B군이 S1군보다 통계학적으로 높게 나타났으며, M4B군과 M5B군은 각각 M4군과 M5군 보다, 그리고 S5B군은 S5군 보다 통계학적으로 낮게 나타났다 (p < 0.05). 본 연구를 종합하여 보면, 복합레진의 종류에 따라 표면조도의 순서는 다르게 나타났고, polyester strip 하에서 복합레진이 중합된 경우 가장 낮은 표면조도 값과 평활한 표면을 제공하였으며 전반적으로 finishing bur는 가장 높은 Ra 값과 거친 표면을 제공하였다. Enhance, Astropol, carbide finishing bur로 연마한 표면은 레진전색제의 사용으로 평활한 표면을 얻을 수 있었지만, polyester strip과 Sof-Lex disc로 얻어진 표면은 레진전색제의 사용으로 표면조도의 개선이 이루어지지 않았다. The purpose of this study was to evaluate the difference of surface roughness of composite resin according to composite resin type, polishing methods, and use of resin sealant. Two hundred rectangular specimens, sized 8 × 3 × 2 mm, were made of Micro-new (Bisco, Inc., Schaumburg, IL, U.S.A) and Filtek Supreme (3M ESPE Dental Products, St. Paul, MN, U.S.A.), and divided into two groups; Micronew-M group, Filtek Supreme-S group. Specimens for each composite group were subdivided into five groups by finishing and polishing instruments used; M1 & S1 (polyester strip), M2 & S2 (Sof-Lex disc), M3 & S3 (Enhance disc and polishing paste), M4 & S4 (Astropol), and M5 & S5 (finishing bur). Polished groups were added letter B after the application of resin surface sealant (Biscover), eg, M1B and S1B. After specimens were stored with distilled water for 24 hr, average surface roughness (Ra) was taken using a surface roughness tester. Representative specimens of each group were examined by FE-SEM (S-4700: Hitachi High Technologies Co., Tokyo, Japan). The data were analysed using paired t-test, ANOVA and Duncan's tests at the 0.05 probability level. The results of this study were as follows; 1. The lowest Ra was achieved in all groups using polyester strip and the highest Ra was achieved in M5, S5 and M5B groups using finishing bur. On FE-SEM, M1 and S1 groups provided the smoothest surfaces, M5 and S5 groups were presented the roughest surfaces and voids by debonding of filler on the polished specimens. 2. There was no significant difference in Ra between Micronew and Filtek Supreme before the application of resin sealant, but Micronew was smoother than Filek Supreme after the application of resin sealant. 3. There was significant corelation between Ra of type of composite resin and polishing methods before the application of resin sealant (p = 0.000), but no significant interaction between them after the application of resin sealant. On FE-SEM, most of composite resin surfaces were smooth after the application of resin sealant on the polished specimens. 4. Compared with before and after the application of resin sealant in group treated in the same composite and polishing methods, Ra of M4B and M5B was statistically lower than that of M4 and M5, and S5B was lower than that of S5, respectively (p < 0.05). In conclusion, surface roughness by polishing instruments was different according to type of composite resin. Overall, polyester strip produced the smoothest surface, but finishing bur produced the roughest surface. Application of resin sealant provided the smooth surfaces in specimens polished with Enhance, Astropol and finishing bur, but not provided them in specimens polished with Sof-Lex disc.

지방자치단체의 관광이벤트사업에 대한 탐색적 연구 : 광주·전남지역을 중심으로 forcused on Gwangju city · Jeonnam province

정영이,이수범,신민자 慶熙大學校 觀光産業情報硏究院 2002 觀光産業情報論集 Vol.3 No.-

With the age of local autonomy, the tourism-event(festival) is actively utilized as the one of strategies to vitalize the region. So, the importance and effectiveness of the tourism-event has been revaluated, new tourism-event has been developed to maximize the effectiveness of local vitality, and the existent festival program has been reorganized for the better performance. The objective of this study were to examine the relationships of improving the image of local culture and vitalizing the local tourism business, and attracting tourist with the local development. The sample of the research was explored by local tourism-events such as "Yeongam WANG-IN Culture Festival", "Jindo Yeongdeung Festival", "Gangjin celadon Culture Festival", and "Gwangju Kimchi Festival" which were held in Gwangju·Jeonnam and were sponsored by the Korea National Tourism Organization. The results of this study may suggest useful management implications for tourism-event.

서울 지역 여자대학교 집단에서 월경전기 증후군 및 월경전 불쾌기분장애의 유병율

정범석,이철,이지현,서민경,한오수,김창윤 大韓神經精神醫學會 2001 신경정신의학 Vol.40 No.4

연구목적 : 가임기 여성의 75%가 하나 이상의 월경전기 증상을 경험하며, 일상생활에 지장을 주는 경우도 3∼8%에 이르지만 한국 여성을 대상으로 한 월경전기 증후군(Premenstrual syndrome : PMS)및 월경전 불쾌기분장애((Premenstrual dysphoric disorder : PMDD)에 대한 역학 조사는 부족한 실정이다. 따라서 본 자들은 한국에서의 유병율, 증상의 양태 및 삶에 미치는 정도 등의 역학 조사를 통해 월경전 불쾌기분장애에 대한 올바른 정보를 제공하고 관련 임상 연구의 기초자료로 활용하고자 본 연구르 시행하였다. 방 법 : 6개의 서울 소재 4년제 여자대학교에서 무작위 군집표본 추출로 선택된 4개 학교의1∼3학년 학생 중 무작위 군집표본추출을 통해 선택된 1419명을 대상으로 하였다. DSM-IV진단기준을 근거로 저자들이 제작한 6점 척도의 설문지가 사용되었으며, 난포기에 비해 황체기에 75%이상 악화된 경우 증상이 있다고 판정할 경우의 월경전 불쾌기분장애의 유병율을 조사하였다. 월경전 불쾌기분장애 집단과 증상은 있으나 진단기준에 부합되지 않는 월경전기 증후군 집단으로 나누어 증상 양태, 식습관 변화, 생활에 미치는 영향 정도 등을 비교하였다. 결 과 : 부적절한 자료 198명(13.9%), 주요 신체 혹은 정신질환을 앓은 경우 등 선정 배제 기준에 속하는 경우 348명(24.5%)을 제외한 873명이 최종 분석되었다. 873명 중 월경전 불쾌기분장애의 유병율은 5.0%이었으며, 월경전기 증후군의 유병율은 83.3%이었다. 가장 흔한 증상은 월경전기 증후군 집단의 경우 신체증상(54.6%)이었고, 월경전 불쾌기분장애 집단의 경우 '지속적으로 짜증난다'(95.5%)이었다. 월경전 불쾌기분장애 집단의 88.6%에서 증상 때문에 한 달에 1∼2회 이상 일이나 공부에 지장이 초래되었다. 결 론 : 한국 여자대학생 집단이 경험하는 월경전 불쾌기분장애의 유병율과 증상 양상은 외국과 비슷하다. 아울러 월경전 불쾌기분장애 집단은 월경전기 증후군 집단에 비해 기분 증상이 더 흔하고 심하는 점에서 월경전 불쾌기분장애는 월경전기 증후권과 구별되는 하나의 질환 상태임을 시사한다. Objects : More than 75% of women have been reported to experience premenstrual symptoms and three to eight percent of them suffer from premenstrual dysphoric disorder(PMDD). But little is known about prevalence of premenstrual syndrome(PMS) and PMDD and PMS in Korean women who sttended universities at the time of survey. Methods : One thousand four hundred and nineteen subjects were randomly selected with cluster sampling methods among four women's university in Seoul. We measured the severity of premenstrual symptoms using six-point rating scale developed by the authors on the basis of the research criteria for PMDD in DSM-IV. Premenstrual worsening of symptoms was defined as increase in symptom scores of more than 75% from follicular to luteal phase score. And also, we compared characteristic symptoms, eating behaviors, and functional impairments between PMS and PMDD. Results : After excluding inadequate data according to our exclusion criteria, 873 of 1419 subjects(61.5%) were included in the estimation of the prevalence of PMS and PMDD. Each prevalence rate of PMS and PMDD was found to be 83.3% and 5.0% on the basis of 75% change method respectively. The most frequent symptom was physical symptoms such as headache or breast tenderness(56.4%) in PMS and 'felt irritable'(9.5%) in PMDD. Of the 44 subjects with PMDD, 39(88.6%) reported to have experienced the impairment in work or school at least once per month because of premenstrual symptoms. Conclusions : Our results indicate that prevalence and symptoms of PMDD in Korean university women are similar to those in western culture. The finding that mood symptoms were more frequent and severe in PMDD than in PMS implies that the former may be a disordered condition to be distinguished from the latter physiologic one.

저온저장 및 후숙제어를 위한 단감 성장기의 숙도 변화

김민용,안창범,강정길 여수대학교 1998 論文集 Vol.13 No.2

As Persimmon is post-ripenning fruit, it is able to storaging in low temperature. In this studt, chemical and physical properties of Persimmons were measured during ripenning period to obtaine basic data for storaging and controlling post ripenning in low temperature. The results of obtained were summarized as follows : 1. Ilmoke showed higher in weight, Dangdo. vit. C. But water content was lower. As a results, It is not fitted to post-ripenning 2. Buyu and Jearae were lower in weight and Dangdo, but contents of water and vit.C were higher. Quality is able to enhanceing during cold storage and post-ripenning. 3. Sechon is the highest in Dangdo, and It is not fitted to cold storage and post-ripenning. In this study, consulsionly, Buyu and Jearae were fitted to cold storage and post-ripenning, and able to enhancing quality.

만성 신부전환자의 혈청 갑상선홀몬 농도의 변화

김민범,이정호,신영태,김삼용,노흥규 충남대학교 의과대학 지역사회의학연구소 1989 충남의대잡지 Vol.16 No.1

To investigate the thyroid hormonal changes and their mechanisms in patients with chronic renal failure (CRF), the author measured serum levels of the thyroid hormones using radioimmu-noassay and calculated each ratio of serum thyroid hormones in 17 patients with CRF. Ten patients were on hemodialysis therapy and seven patients had not undergone hemodialysis. The following results were obtained. 1) The serum T3 and T4 concentrations were significantly lower in patients with CRF(0.7±0.47㎍/1 and 5.53±2.95㎍/dl, respectively) than in normal subjects(1.21±0.18㎍/1 and 9.46±1.92㎍/dl, respectively. 2) The serum FT4(free T4) concentration was significantly lower in patients with CRF(1.20±0.50μu/dl) than in normal subjects(1.01±0.48μu/dl). 4) The serum rT3(reverse T3) concentration in patients with CRF(285.7±148.1pg/ml) tends to be greater than normal subjects(204.6±43.3pg/ml). But is was not significant statistically. 5) The calculated serum concentration ratio of T3/T4 in patients with CRF(0.17±0.04: presented as Mean±SEM) did not differ significantly from that of normal subjects(0.13±0.01). But the serum concentration ratio of rT3/T4 in patients with CRF(78.78±18.98) was significantly greater than in normal subjects(21.90±1.04). 6) There was no significant difference in thyroid functions between male and female patients with CRF, and also between patients on hemodialysis and non-dialyzed uremic patients. It was concluded that the serum T3, T4, FT4 concentration were significantly decreased in patients with CRF, while the serum TSH concentration was significantly increased. The calculated serum concentration ratio of rT3/T4 was also significantly increased in patients with CRF. Thus is appears that abnormalities found in patients with CRF may reflect both the possible intrathyroidal abnormalities and alteration of serum rT3 metabolism with increased peripheral generation of rT3 from T4.

열대지방사람들의 땀샘의 밀도와 기능

이정범,배준상,민영기,양훈모 순천향의학연구소 2004 Journal of Soonchunhyang Medical Science Vol.10 No.1

Tropical inhabitants are able to tolerate heat through permanent residence in the tropics. To clarify the peripheral mechanisms of the suppressed thermal sweating in tropical natives, sweating responses to acetylcholine (ACh), a primary transmitter of the sudomotor innervation, were compared between healthy Korean and African males. ACh was iontophoretically administered on the forearm. Directly activated and axon reflex-mediated sweat responses were evaluated by quantitative sudomotor axon reflex test (QSART). The sweating of axon reflex (nicotinic receptor) onset-time was 0.42 min shorter (P<0.01) and the sweat volumes (muscarinic receptors) were 75% (P<0.01) and the ACh sweat volumes of axon reflex (nicotinic receptor) 79% higher (P<0.01) in the Koreans than the Africans. Iodine-impregnated paper method revealed that sweat gland density was 45.1% higher (P<0.01) and sweat gland output per single gland was 18.4% larger (P<0.001) in the Koreans compared to the Africans. These results indicate that suppressed thermal sweating in Africans is, at least in part, attributed to the suppressed glandular sensitivity to ACh through both recruitment of sweat glands and sweat output per each gland.

충청지방에서 발생한 한국형 출혈열 104예의 임상적 고찰

이정호,신승훈,김민범,신영태 충남대학교 의과대학 지역사회의학연구소 1989 충남의대잡지 Vol.16 No.1

A clinical study was carried out on 104 cases of Korean Hemorrhagic Fever who were admitted to Chungnam National University Hospital from Januray 1982 to December 1988. 104 cases among the patients were confirmed -by serologic test. The resutls were as follows: 1. The peak monthly incidence was November. The peak age incidence were 5th and 6th decade and male to female ratio was 2.5:1. The occupation of the patients were variable, but farmer was the most prevalent. 2. Most patients entered hospital in oliguric stage and the mean duration from onset of symptoms to admission was 5 days. 11 OUT OF 104 patients were non-oliguric stages. 3. The reguent and pertinent symptoms and signs were fever, chills, general myalgia, abdomical patin, back pain, abdominal and costovertebral angle tendernes sand bleeding tendencies. 4. The important laboratory findings for diagnosis were leukocytosis, thrombocytoenia, elevated blood urea nitorgen and creatinin. 5. Hypertension was noticed transiently in 42 cases(40.4%). Other complications were infection, melena, pleural effusion, convulsion and premature repture of membrane in pregnancy in 4, 3, 3, 2 and 1 cases respectively. 6. 26 patients who showed severe acute renal failure were treated with acute hemodialysis while 78 patients with conservative treatment. All patients except one who developed unidentified cerebral dysfunction were discharged with improvement.

정신분열병에 대한 리스페리돈의 효과 및 안정성

이민수,김용구,김영훈,연병길,오병훈,윤도준,윤진상,이철,정희연,강병조,김광수,김동언,김명정,김상훈,김희철,나철,노승호,민경준,박기창,박두병,백기청,백인호,손봉기,손진욱,양병환,양창국,우행원,이정호,이종범,이홍식,임기영,전태연,정영조,정영철,정인과,정인원,지익성,채정호,한상익,한선호,한진희,서광윤 大韓神經精神醫學會 1998 신경정신의학 Vol.37 No.1

연구목적 : 본 시험의 목적은 임상시험 시작전에 연구자들을 대상으로 PANSS Workshop을 통하여 PANSS, ESRS에 대한 국내에서의 표준화 작업을 구축하고 새로운 정신병 치료제인 리스페리돈의 효과와 안정성을 재확인하여 리스페리돈 사용에 대한 적정화를 이루는데 있다. 연구방법 : 1996년 4월부터 1996년 9월까지 국내 39개 대학병원 정신과에 입원중인 혹은 증상이 악화되어 입원하는 정신분열병 환자 377명을 대상으로 다시설 개방 연구를 시행하였다. 1주일간의 약물 배설기간을 가진후, 리스페리돈을 8주간 투여하였고, 기준점, 1주, 2주, 4주, 그리고 8주후에 평가되었다. 용량은 제1일에는 리스페리돈 1mg씩 1일 2회, 제2일에는 2mg씩 1일 2회, 제3∼7일에는 3mg씩 1일 2회 투여하였다. 이후 환자의 임상상태에 따라 임의로 증량할 수 있으며, 최대 일일 16mg을 초과하지 않도록 하였다. 추체외로 증상을 조절하기 위한 투약을 허용하였다. 임상증상 및 부작용의 평가는 PANSS(Positive and Negative Syndrome Scale), CGI(Clinical Global Impression) 그리고 ESRS(Extrapyramidal Symptom Rating Scale)을 사용하였다. 연구결과 : 377명중 343명(91%)이 8주간의 연구를 완결하였다. 치료 종결시점인 8주후 PANSS 총점수가 20% 이상 호전된 경우를 약물 반응군으로 정의할때, 약물반응군은 81.3%였다. 리스페리돈에 반응하는 예측인자로는 발병연령, 이전의 입원 횟수, 유병기간이 관련 있었다. 리스페리돈은 1주후부터 PANSS양성, 음성, 및 일반정신병리 점수상에 유의한 호전을 보여 효과가 빨랐다. CGI의 경우도 기준점에 비해 1주후부터 유의한 감소를 나타내었다. ESRS의 경우, 파킨슨 평가점수는 기준점과 비교해 투여 1주, 2주, 4주후 유의하게 증가되었다가 8주후 기준점과 차이가 없었다. Dystonia 평가점수는 1주후만 유의한 증가를 보였으며, dyskinesia 평가점수는 유의한 차이가 없었다. 혈압, 맥박수의 생명징후 및 일반 혈액학 검사, 생화학적 검사, 심전도 검사에서 유의한 변화는 없었다. 결 론 : 이상의 다시설 개방 임상 연구를 통해 리스페리돈은 정신분열병 환자에서 양성증상뿐만 아니라 음성증상 및 전반적인 증상에도 효과적인 것으로 사료된다. 보다 명확한 평가를 위해서는 다른 항정신병약물과의 이중맹검 연구가 필요할 것으로 생각되며, 또한 장기적 치료에 대한 평가도 함께 이루어져야 하겠다. Objective : The purpose of this study was to investigate the efficacy and safety of risperidone in the treatment of Korean schizophrenic patients. Method : This multicenter open study included 377 schizophrenic patients drawn from 39 university hospitals. After a wash-out period of 1 week, the schizophrenic patients were treated with risperidone for 8 weeks and evaluated at 5 points ; at baseline, and 1, 2, 4 and 8 weeks of treatment. The dose was increased from 2mg/day(1mg twice daily) to 6mg/day(3mg twice daily) during the first week and adjusted to a maximum of 16mg/day over the next 7 weeks according to the patient's clinical response. Medication to control extrapyramidal symptoms was permitted. The psychiatric and neurological status of the patients was assessed by PANSS, CGI, and ESRS scales. Results : 343(91%) of 377 patients completed the 8-week trial period. Clinical improvement, as defined by a 20% or more reduction in total PANSS score at end point, was shown by 81.3% of patients. The predictors of response to risperidone were associated older age, shorter duration of illness, fewer previous hospitalization. Risperidone had rapid onset of action ; a significant decrease of the total PANSS and three PANSS factor(positive, negative, general), and CGI was already noticed at the end of first week. For the ESRS, parkinsonism rating scores were significantly increased until week 4 comparing with baseline. Dystonia rating scores were significantly increased until week 1, and dyskinesia rating scores were not significantly changed during the study. Laboratory parameters including vital sign, EKG, hematological, and biochemical values showed no significant changes during the trial. Conclusions : This study suggests that risperidone is generally safe and effective against both the positive and negative symptoms in our group of patients.