미세절제술과 비교 유전자 보합법에 의한 각종 종양에서의 유전자 변화에 관한 연구

구선회,신소영,임춘화,전영미,이윤이,김진만 충남대학교 의과대학 지역사회의학연구소 2000 충남의대잡지 Vol.27 No.2

For the evaluation of oncogenesis, progression and prognosis of cancer, CGH is an important technique, because this technique is economic due to utilization of only one probe and lack of culture, screening mathod of whole genome and possibility of retrospective and prospective study. By the CGH, genornic variation of 20 breast cancer tissues, 23 stomach cancer tissues and 16 bladder cancer tissues were analyzed. The results were as followes ; 1. breast cancers The CGH results showed gains on chromosomes 8q(40%), lq(30%), 17q(15%), 20q(15%), 18q (15%), 5p(15%), and 13q(15%). The Deletions were on chromosomes 17p(45%) and 22q(20%). High-level amplifications(green/red ratio >1.5) were noted on chromosomes 1p31, iq, 3q25-qter, 5p, 7q31-qter, 8q, 9q22-qter, 10p, l1p, 11q22-qter, 12p, 12q24, 14g21-qter, 15q23-qter, 17q, 18p, 18q12-qter, 20p, and 20q. By comparison with infiltrating ductal carcinoma, the two medullary carcinomas showed high-level amplification on chromosomes iq3l, lq, 8q, 10p, 11p and 12p. 2. stomach cancers 1) Usual amplification sites of genome were lq, 13q, 17q, 20p,q. 2) 17p was the most common deletion site. The other sites of the deletion were lq, 4q. 3) In intestinal type of stomach cancer, genomic variation is more common than diffuse type. 4) In the cases of no evidence of lymph node metastasis, deletion of 17p is absent but amplification of 8q is obvious in the case of lymph node metastasis. 3. bladder cancers Common amplification of copy numbers of DNA sequences by CGH were seen at 1q, 3q, 4q, 5p, 6pq, 7p, 8q, 11q, 12q, 13q, 17q, 18q and 20pq(more than 20% of cases). High level amplification was noted at 1p32, 3p2l, 3q24, 4q26, 8q21-ter, 11q14-22, 12q15-21, 12q21-24, 13q 21-31, 17q22, and 18q22. Deletions were noted at 2q21-qter, 4q13-23, 5q, 8p12-22, 9pq, 11p13-15 (more than 20% of cases).

ATB Expert System을 이용한 항균제 감수성 검사에 관한 연구

구선회 충남대학교 의과대학 지역사회의학연구소 1993 충남의대잡지 Vol.20 No.2

The success of therapy depends on the selection of antibiotics by the clinician in the case of infection. performance of susceptibility test provides the clinician with a prediction of the activity of an antibiotics. but nowadays resistance mechanisms of increasing complexity are found in a growing number of microbial species. So, for the validation of the antibiotic susceptibility requires the more complete interpretation, using all the knowledge accumulated on antibiotic resistance mechanism. we could detect the resistance mechanism, such as acquire resistance, crossresitance, associated resistance by the ATB expert system which was deviced by artificial knowledge. also we could detect the technical error, incomplete expression of resistance, rare phenotype and impossible phenotype. but there are some limitation for complete adoption in clinical fields because only a few rules were used and not so simple for all members of the microbiology laboratory.

외국어 학습과 동기 : 연구의 동향 및 쟁점 A Review of the Issues

구광현,곽선연 안양대학교 1997 논문집 Vol.17 No.-

This paper addressed the issue of motivation in foreign language learning in five main categories. First of all, the importance of motivation in language learning was stressed with a vivid example which could overcome unfavorable circumstances in other aspects of language learning. Secondly, a layman's or 'naive' view of the problem was introduced, and general psychological viewpoints on motivation were reviewed. Thirdly, earlier and contemporary views of specific motivation for language study were reviewed. Particularly, the work of Robert Gardner was analysed in detail. Fourthly, Gardner's work was critically evaluated and the major issues in the study of motivation were discussed. Finally, several attempts to relate the findings on individual differences in motivation to a wider educational framework were discussed with regards to further studies.

형광 염색체 In Situ Suppression Hybridization의 임상적 응용에 관한 연구

구선희 충남대학교 의과대학 지역사회의학연구소 1992 충남의대잡지 Vol.19 No.2

A method for chromosome specific staining and its use in clinical cytogenetics is evaluated. Non isotopic fluorescent in situ hybridization provides a fast method for detection of specific nucleic acid sequences. We have used the single color fluorescent in situ hybridization with a α-satellite probe, unique sequence, microdissected probe to investigate the feasibility of simultaneous assessment of numerical and structural chromosome aberrations. When we used the α-satellits probe, Under condition of low stringency, cross hybridization with other subpopulations of the alpohoid family occurs in the whole chromosome and numerous hybridization sites are detected over metaphase. But we could findout the 3 signals in interphase chromosome of Down syndrome we could detect the signals which have been suspicted a deletion point with the unique sequence probe. We could delineate the X-Y translocated region by a microdisscted Y probe. From the above results, it can be a novel method for detection of cytogenetic abnormalities in the clinical cytogenetic field.

Fluorescence in situ Hybridizaton에 의한 염색체 이상의 절단점의 규명

구선회,송인숙 충남대학교 의과대학 지역사회의학연구소 1996 충남의대잡지 Vol.23 No.2

Fluorescence in situ hybrididzation(FISH) is a novel molecular genetic technique. It used for gene mapping in researches and in clinical cytogenetic fields. We perfomed FISH using various kinds of probes for the determination of breakpoints in chromosomal abnormalities. Following results were obtained: 1. Determination of breakpoint in complex translocations. FISH results with chromosome 14/22 centromere probe showed that the breakpoint was 14q11 in the patient with corpus callosum agenesis and complex transocation. 2. Determination of breakpoint of inv(16) in acute myelomonocytic leukemia. FISH results with inv(16) probe showed that the breakpoint was between 16pll and p13 in an abnormal inv(16) chromosome. 3. Determination of breakpoint of dup(17q) in acute myelocytic leukemia. FISH results with iso 17q probe showed that the breakpoint was 17q21 and the duplicated segment from q21-q25. 4. Determination of breakpoint of isodicentric X chromosome. FISH results of Turner syndrome with two iso Xq chromosome using a-satellite X centomere probe showed that the breakpoint was in proxim portion of centromere. From the above results, we could easily find out the breakpoints of chromosomal abnormalities by FISH. It was helpful for the tracing of pathogenesis of genetic disease and cancers.

CALPUFF model과 ISCST3 model을 이용한 악취모델링 기법 연구

구윤서,김성태,김용규,송선호 안양대학교 산업기술연구소 2002 自然科學硏究 Vol.9 No.-

악취는 인간의 후각기관에 의해 감지되기 때문에, 대상 지역의 평균농도(Average Concentration) 보다는 악취의 특성상 순간적인 농도인 첨두농도(Peak Concentration)가 중요한 의미를 갖는다. 따라서 이것을 모델링에 적용하기에는 많은 어려움이 있다. 이를 해결하는 것이 이 연구의 주된 과제라 할 수 있다. 현재 국내에서 환경영향평가에 많이 사용되고 있는 ISCST3 Model (Industrial Source Complex Short Term 3 Model)과 CALPUFF Model (California Puff Model)을 사용하여 첨두농도(Peak Concentration)를 고려한 관능악취도(Odor Intensity)를 두 가지 방법으로 산정 하였다. 첫 번째로는 오염원에서 배출되는 물질의 농도를 이용하는 방법과 다른 하나는 종합악취도 개념으로 악취농도(Odor Concentration)를 이용하여 관능악취도를 산정하는 것이다. 위의 두 가지 방법을 ISCST3 Model과 CALPUFF Model에 적용하여 모델링을 수행한 후 나온 기여농도를 첨두농도로 환산한 후 관능악취도를 계산하였다. 이와 같은 이론 배경을 바탕으로 현장 적용성을 파악하기 위해서 수도권매립장의 한 지점에서 가상의 가스배제공이 있다고 가정하고, ISCST3 Model과 CALPUFF Model에 동일한 조건으로 모델링을 수행하여 매립장 주변에 미치는 관능악취도를 각각 산출해 비교 검토하였다. 그 결과 두 모델에서의 차이는 크지 않았으나 기여농도와 첨두농도 사이에는 상당한 차이를 보였다. 결과적으로 악취모델링을 수행함에 있어서 기여농도만을 고려하는 것은 관능악취도를 과소평가 할 우려가 있으므로 배출원 특성을 고려한 첨두농도로 관능악취도를 평가하는 것이 타당하다고 판단된다. In order to manage the odor related public complains effectively, the estimation of peak ground-level concentration which is capable of predicting the intermittent maximum response less than 10 seconds is very important. However, peak rather than 3 minute or 1 hour average concentrations are not easily predicted by current air dispersion models such as SCST3 (Industrial Source Complex Short Term - version 3) and CALPUFF (CALifornia Puff) models. According to the field and wind tunnel measurements, the intensity of the peak concentration depends on the emission source type, downwind distance and atmospheric stability. The odor modeling methods to predict the peak concentration were reviewed and the detailed procedure for the odor modeling was explained in this study. In order to compare the conventional air dispersion models with the oder models, the odor concentrations with various modeling techniques under the identical emission and meterological conditions were calculated. The peak concentration shows highest value than the 3 minutes or 1 hour average concentrations. The predicted odor concentrations by the CALPUFF model are higher than those predicted by the ISCST3 model since the CALPUFF model reflects the time-dependent movement of the plume. It is recommended that using the peak concentration with the CALPUFF model rather than 3 minutes or 1 hour average concentrations is more reasonable approaches to manage the odor problems.

창의적 문제해결을 위한 웹기반 교수-학습 모형과 학습 환경 설계 : 수학교과에서의 예시를 중심으로

구양미,김영수,노선숙,조성민 이화여자대학교 사범대학 교과교육연구소 2006 교과교육학연구 Vol.10 No.1

본 연구의 목적은 지식기반사회의 학습도구로서 그 중요성이 증대되고 있는 인터넷과 웹을 활용하여 창의성 계발을 돕는 교수-학습 모형과 웹기반 학습 환경을 설계하는 데 있다. 본 연구에서 웹은 학습도우미(learning agent)로서 문제해결 학습상황에서 의사소통도구, 발견과 탐색도구, 지식과 경험 구성의 도구로 상정되었다. 즉, 중등 수학교과를 사례로 채택, 문제해결 학습상황에서 생각 5단계 모형(생각 열기, 생각 쌓기, 생각 나누기, 생각 다지기, 생각 펼치기)을 토대로 각 단계별로 문제의 구조 유형에 따른 사고 전략과 이에 상응하는 학습자와 교수자 활동, 웹기반 활동을 포함하는 웹기반 교수-학습 모형을 설계하였다. 설계된 웹기반 교수-학습 모형에 따라 실제 교수-학습활동 계획안을 개발하고, 창의적 문제해결 환경 지원을 목적으로 한 웹사이트를 설계하였다. 본 연구의 결과물인 웹기반 교수-학습 모형과 학습 환경은 창의적 문제해결을 위한 핵심요소로 간주되는 확산적 사고, 비판적 사고 및 교과지식이 조화롭게 활용될 수 있도록 하는데 그 주안점을 두고 있다. The purpose of this study is to design a web-based teaching and learning model and learning environment for creative problem solving in Mathematics. In this study, World Wide Web is considered as a learning agent assisting learning process as communication tools, inquiry tool, and knowledge construction tools in problem solving learning environments. The web-based teaching and learning model was developed based on "Five Stage Thinking Model" that consists of uncover ideas, accumulate ideas, share ideas, ensure ideas, and unfold ideas. According to the steps of the model, a web-based lessen plan on coordinates in middle school mathematics was developed. Finally, a web-based their divergent thinking, critical thinking and subject knowledge for mathematical problem solving creatively. Some sample web pages were suggested for developing a web site based on the purposed model and the lessen plan.

니페디핀과 β-시클로덱스트린 유도체류와의 포접복합체 형성 및 고체분산체 함유 마이크로캅셀의 제어방출

전인구,최선애 同德女子大學校 1997 同大論叢 Vol.27 No.1

To overcome poor dissolution property of nifedipine (NFD), which is used in the treatment of ischemic heart disease and hypertension as a calcium antagonist, effects of β-cyclodextrin (β-CyD) derivatives on the solubility of NFD in aqueous buffered solutions were investigated. The aqueous solubility of NFD increased linearly with increase in the concentration of CyDs, showing an A???-type phase solubility diagram. The stability constant of NFD-CyD complexes was in the order of 2-hydroxypropyl-β-cyclodextrin (HP-β-CyD) <β-CyD ≪diemthyl-β-cyclodextrin (DM-β-CyD), and the solubilizing ability of the β-CyDs was also in the same order. The dissolution rate of NFD-DM-β-CyD (1 :2 molar ratio) or NFD-β-CyD (1 :2 molar ratio) solid dispersions was much faster than those of NFD-β-CyD physical mixtures and the drug alone. Therefore, solid dispersion system of NFD with β-CyD (1 : 2 molar ratio) was prepared by solvent evaporation and encapsulated with Eudragit RS as the coat at core to wall ratios of 1 : 0.1 and 1:0.3 (w/w) using phase separation method by non-solvent addition. All the Eudragit RS microcapsules of NFD-β-CyD solid dispersion at core to wall ratio of 1:0.1 or 1:0.3 (w/w) were small and uniform. Although dissolution of NFD from Eudragit RS microcapsules was retarded with increasing the ratio of core wall from 1:0.1 to 1:0.3 (w/w), the release rate was sustained in a much higher concentration than those of drug alone and commercial sustained release NFD tablets.

생약재 혼합급여가 웅추의 정액성장과 장기발육에 미치는 영향

오선균, 조성구 忠北大學校 1986 論文集 Vol.32 No.-

-본문참고-

관상 동맥 질환에서 아포 E 지단백 유전자 다형성과 혈청 지질치와의 관계

곽선영,김성구,정호석,이유경,이광희,김철현,최태명,현민수,권영주 순천향의학연구소;Soonchunhyang Medical Research Institute 2000 Journal of Soonchunhyang Medical Science Vol.6 No.1

Background and aims: The Apolipoprotein E is a ligand of both the protein component LDL receptor as well as the apo E LDL-Receptor related protein (LRP). It modulates the receptor binding of lipoproteins, with the apolipoprotein E found on cell surfaces as its component, thus serving an important role in the lipid metabolism by carrying out the intracellular transport of cholesterol in lipoproteins. The gene for apolipoprotein E is the product of three common genotypes as well as many more rare alleles. The common genotypes are ε2, ε3, and ε4, and are expressed in the three phenotype isoforms of E2, E3, and E4. In the event that E4 is the main component, a rise in the cholesterol level, as the result of down-regulation of the LDL receptor, is observed. Therefore, those samples with E4 genotypes are known to be in much higher risk of coronary artery disease than those with ε3/ε3, while those with ε2 are in low risk (with the exception of hypertiglyceremai Ⅲ). The aim of this study is to analyze in patients with ischemic heart disease the role of aplipoprotein E alleles in order to seek its correlation with coronary artery disease, as well as to seek whether the polymorphism of apo E produces any differences in the severity of coronary artery disease according to plasma lipid levels. Methods: The subjects for study were 273 patients admitted to the Internal Cardiology Division of the Soonchunhyang University Hospital form December 1998 to February 1999. The subjects were divided into the two groups of which one was ischemic heart disease (IHD) experiment group totaling 105 (avg.60.1 years of age, male/female ratio = 69/36) and the control group totaling 168 (avg. 59.7 years of age, male/female = 73:95). The coronary angiogram was given to 127 subjects, and of this total, 94 have developed significant stenosis in the coronary artery. The stages of the analyzing of the apo E phenotype was first, the separation of DNA from the blood samples, subjecting it to the PCR from with 228 base pairs of expanded products were obtained. The band was determined by means of the reverse hybridization principle on the nitrocellulose strip. Results: From the 105 patients the distributions of apo E phenotypes were as follows: ε3/2(5.7%), ε4/2(1.9%), ε3/3(70%), ε4/3(20%), ε4/4(1.9%). The relative frequencies of each allele are as follow: ε2 (0.038), ε3 (0.833), ε4 (0.128). The results show as follows: ⅰ) The IHD experiment group to have a higher occurrence of ε4/3 phenotypes as well as ε4 alleles than the control group. ⅱ) Both the control group and IHD group showed the largest distribution of ε3/3 for phenotypes, and ε4 for alleles. ⅲ) The IHD group showed less ε2/3 phenotypes as well as significantly less allele frequency of ε3 in comparison to the control group. ⅳ) the IHD group showed a much lower level of HDL in comparison to the control group, while the LDL was significantly higher; samples including the apo ε2 showed a significantly higher level of HDL than those without. Among the control group, samples including apo ε2 showed a significantly higher level of TG (triglyceride) than samples without. No significant difference was found between the experiment apo ε4 sample and the control plasma lipid sample. ⅴ) No significant correlation was found between an apo E polynorphism and the number of involved arteries of a coronary angiogram. Conclusion: Between the experiment IHD group and control group were found differences in the frequency of alleles. The polymorphism of apo E alleles may contribute as a risk factor to the development of heart disease by involving itself in the metabolism and modulation of plasma lipids.