Slide Session : OS-IFD-07 ; Infectious Disease : In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus

( Myung Jin Lee ),( Kye Hyung Kim ),( Jong Youn Yi ),( Su Jin Choi ),( Chung Jong Kim ),( Nak Hyun Kim ),( Kyoung Ho Song ),( Pyoeng Gyun Choi ),( Ji Hwan Bang ),( Wan Beom Park ),( Eu Suk Kim ),( San 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus Myung Jin LEE1, Kye-Hyung KIM1, Jongyoun YI2, SuJin CHOI1, Chung-Jong KIM1, Nak- Hyun KIM1, Kyoung-Ho SONG1, Pyoeng Gyun CHOI1, Ji-Hwan BANG1, Wan Beom PARK1, Eu Suk KIM1, Sang-Won PARK1, Hong Bin KIM1, Nam Joong KIM1, Myoung- Don OH1 Seoul National University College of Medicine, Korea1, Pusan National University School of Medicine, Korea2 Background: Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease caused by a novel Bunyavirus, severe fever with thrombocytopenia syndrome virus (SFTSV). No effective antiviral therapy is proven yet, but clinical use of ribavirin (RBV) has been tried. We investigated the antiviral effect of RBV against SFTSV in vitro. Methods: To test for cytotoxicity of RBV, Vero cells were treated with different concentrations of RBV (3.90 to 500 μg/mL, two-fold dilution) and analyzed by cell viability MTS assay 48h post-infection. To determine antiviral activity of RBV against SFTSV, Vero cells were infected with SFTSV strain Gangwon/Korea/2012 at 100 TCID50 (50% tissue culture infective dose) per well in a 96-well plate, and RBV was added at the concentrations showing no or minimal cytotoxicity. Viral RNAs were extracted from the culture supernatants and quantifi ed using one-step real-time reverse transcription- PCR to amplify the partial large segment of SFTSV. Statistical analysis was done by one-way ANOVA with Tukey`s post hoc test. Results: Cytotoxicity due to RBV was not observed at RBV concentration =31.3 μg/ mL. Viral RNAs at 24h post-RBV treatment were reduced with increasing RBV concentrations (1-32 μg/mL), compared with those of mock-treated cells (P <0.01, Figure). Half maximal inhibitory concentration (IC50) of RBV was 3.69 μg/mL at 24h post-RBV treatment. Conclusions: Our study shows that RBV has antiviral effect against SFTSV in a dose-dependent manner. Further studies are required to evaluate the effi cacy of RBV in SFTS.

저위교합 유구치에서의 공간확장술을 이용한 외과적 접근

방석윤,김은정,김현정,김영진,남순현 大韓小兒齒科學會 2005 大韓小兒齒科學會誌 Vol.32 No.3

저위교합이란 능동적 맹출기 동안이나 이후 해당치아의 교합면 방향으로의 성장이 멈추어 다른 치아들보다 하방에 존재하는 것을 말한다 저위교합치의 결과로 침하치의 만기잔존, 부정교합, 증가된 치아우식 감수성과 치주질환의 가능성, 계승치의 이동 등이 나타날 수 있다. 따라서 조기진단 및 적절한 처치가 필요하다. 저위교합치의 치료는 저위교합 정도나 후속 영구치의 발육정도에 따라 보존에서 발치까지 다양하다. 영구 계승치가 없거나 부분적인 침하치가 치주적으로 건전한 경우, 공간문제를 발생하지 않는 경우 보존적인 접근이 추천되기도 한다. 그러나 계승치가 존재하고 맹출이 지연되는 경우, 국소적인 염증반응과 관련되는 경우 교정적인 치료가 필요한 경우에는 발거가 필요할 수도 있다. 특히 저위교합된 유구치의 상방으로 인접치가 경사되어 악궁장경에 문제를 초래할 위험이 있는 경우 외과적인 발치가 필요할 수도 있으며 경사가 심한 경우 발치를 위한 외과적 접근이 힘들 수 있다. 심하게 경사된 인접치로 인하여 저위교합치의 외과적 발거가 힘들 환아에서 가철성 교정장치를 이용하여 공간확보를 진행 하였으며 보다 용이하게 치아를 발거할 수 있었다. Infraclusion may be defined as teeth that stop their relative occlusal movement in the dental arches during or after the period of active eruption and then remain under the occlusal plane. Delayed exfoliation, malocclusion, increased susceptibility to dental caries and periodontal disease of both the neighboring teeth and retained molar, and dislocation of' the successor are the consequencces of infraclusion of primary molars. Therefore, early diagnosis and appropriate treatments are necessary. The therapeutic approach of' the infracluded teeth varied from preservation to extraction. The teeth with simple infraclusion without any signs of interference with occlusal and jaw development may be examined periodically with fellow-up check and radiographically. However, if the infracluded tooth interferes with normal eruption of successor or shows any sign of delayed resorption, or the tipping of adjacent teeth or supraeruption of op-posing teeth is expected, the teeth inflicted should be extracted and appropriate measures should be provided in order to maintain the normal development of occlusion and dentition. The adjacent teeth which have been collapsed over a infracluded deciduous teeth can disturb the arch length perimeter. In such cases, surgical approach might be necessary, although it would be difficult when teeth are severly leaned. However, an easier surgical access have been obtained by space regaining procedures, in young patients whose arch length has been shortened due to the infracluded teeth.

혈부축어탕의 고지혈증 유발 생쥐 신장내 지방 축적 감소효과

김호현,강윤호,방혜정,김진택,박인식,안상현,이해풍 世明대학교 한의학연구소 1998 韓醫學硏究所 論文集 Vol.1 No.-

Renal tissues of ICR mouse were intraperitoneally injected with Hyulboochucketang(HCE;1.28g/kg/day) after Triton WR-1339(TX;600mg/kg) injection were observed to investigate the lipid accumulation suppressive effect of HCE. The renal tissues were obtained at hour 24,48 and 72 after TX injection that were fixed in fromol-calcium solution and were cryocut. The tissue stained by H&E for general morphology, sudan black B for lipid and perchloric acid-naphthoquinone method for cholesterol. The parietal layer of Bowman's capsule is swelled as cuboidal type at hour 48 after TX injection and the lipid blot and cholesterol particle were noticeably increased in glomerulus than control group. But the parietal layer of Bowman's capsule in HCE treated group was shaped as squamous type that was looked like as normal morphology and the lipid blot and cholesterol particle w3ere considerably decreased in glomerulus than TX group. As results indicated that the accumulation of lipid including cholesterol caused by TX injection were mitigated in renal tissues by the antihyperlipidermic effect of HCE.

健康한 韓國人의 動脈血液 가스 및 酸一鹽基 平衡値에 對한 觀察

崔震守,曺方煥,朴喜淑,金顯承,金瑛,白正敏,徐錫助 순천향의과대학 1979 Journal of Soonchunhyang Medical Science Vol.2 No.3

The purpose of this paper is to make an attempt in this investigation to determine normal adult values of arterial blood gas and acid-base balance in 53 healthy Korean male and female subjects of varying age and then compare with the data obtained by others. Determinations of the acid-base status of the arterial blood were performed at 37℃ by the Astrup method using a radiometer instruments, consisted of BGA2A/3-MK 2 system. The mean and standard errors of each component were as follows: pH 7.42±0.043, oxygen saturation 96.96±0.089%, pO?? 97.66±0.91mmHg, pCO?? 37.08±0.68mmHg, CO?? content 24.29±0.43mM/L, bicarbonate 23.26±0.68mM/L, buffer base 46.56±0.66mEg/L, and base excess -0.024±0.001mEg/S. Compared with normal adult values in literatures, all the components of the data appear to be in the same range without any significant difference.

경호무도로서 태권도 가치와 활용 방안에 관한 연구

양진방,김현호 龍仁大學校 武道硏究所 2004 武道硏究所誌 Vol.15 No.1

In spite of rapidly increasing security demand due to increasing, diversified, brutalized, widely committed and organized crimes caused by recent rapid urbanization, industrialization and information era trend of Korea, efficient prevention activities against crimes becoming tough and distrust on duty performance ability of the police is also increasing because of absolutely insufficient police manpower, budget and outdated obstacles. Therefore, civil security, crime prevention organization in civilian level, seems to play an important role in solving safety problems in people's life and satisfying various security demand of all levels of society caused by industrialization and urbanization. This study is significant in that it could be used as basic data to establish a base for the study and application of taekwondo as a guard martial art, and this study is aimed at examining the value and application plan of taekwondo and application methods of applied skills in case of accidental cases. The results of documentary researches to attain these goals are as follows. First, various training models could be established if lots of efforts in analytic researches on guard situations are made on the basis of spiritual aspect, historical background and established technique system of taekwondo. And as efforts to establish the system of taekwondo as a guard martial art, popularization through the system establishment of guard martial arts with existing taekwondo for a basis, the establishment of taekwondo educational programs as a guard martial art, voluntary efforts of taekwondo people, new thinking and efforts of university students majoring in taekwondo and investment based on field experiences of private and public guard agencies are required. Second, the answer for a question that which martial art is the strongest is meaningless when every martial art reached a stage of the best. But, we need to examine the value of taekwondo as a guard martial art by studying the system of guard martial arts on the basis of taekwondo which is more systematic and has proven its value at home and abroad. Also, the study on the value of taekwondo and its application are required to meet explosively increasing demand in the guard industry at home and abroad and to recreate taekwondo as a guard martial art for the manifestation of perfect guard capability. Third, as the application method of taekwondo in case of accidental cases, a safe distance should be kept so as clients not to be attacked, and in case of the failure in the security of safe distance, attack chances should be prevented by locating guards between clients and attackers. Also, when there are physical actions in attack, guards should block or defend them right after the actions, and when the attack of opponents is excessive, guards should gain supremacy perfectly over the opponents and eliminate the intention of attack so as clients' safety not be threatened anymore. For technical aspects to deal with these situations, physical training and mind training should be conducted for the direct aim of technical training and it is trained by classified techniques such as self-protection technique, practical technique and situation development technique, and as physical strength elements required for health maintenance and physical training, Poomse(formal exercises), kicking and Gyorugi are to be trained for instantaneous reactionary force and quickness.

스키시즌권 구매자들의 스키장별 선호도 조사

장현철,김경식,방준현,최천진,엄정식,김응준,이루지 한국스포츠리서치 2004 한국 스포츠 리서치 Vol.15 No.2

The purpose of this study was to examine the perception and preference of skiers in a bid to provide ski resorts with useful information about successful positioning strategy. The subjects in this study were 9,722 skiers who purchased season tickets from N web site that sold ski season tickets. Their online purchasing route and page view were analyzed to find out what factors affected their purchase of ski season tickets. The findings of this study were as follows: First, 78 percent of the people investigated were make, and 22 percent were female. By age, the largest group was in their 20s(5,344 skiers), followed by those in their 30s(3,434), those in their 40s(631) and teenagers(229). Second, their page view rate with Yangji recorded highest with 24%, followed by Sungwoo Resort with 23% and Chonmasan with 13 percent. Third, by geographic area, the skiers from southern Gyeonggi province bought the largest number of tickets for the 2002/2003 season, followed by Gangweon province residents and northem Gyeonggi province dwellers. In the case of the 2003/2004 season, those from Gangweon provinece pruchased the most tickets, followed by southern Gyeonggi province dwellers and north Gyeonggi province residents.

수부화상의 이차적 치료

김용배,방유현,이진호,박종섭 大韓成形外科學會 1983 Archives of Plastic Surgery Vol.10 No.3

A functionless hand resulting from the sequalae of a thermal burn is very serious. It prevents restoration of socioeconomic behavior. Since the magnitude of this problem is rewards for the most deligent and competent primary care greater. It can not be emphasized too strongly the result of good primary care are always superior to what one can expect from secondary restoration. The pathogenesis of the functionless hand is caused by a triad of factors resulting from thermal injury that together lead to the rapid stiffening of joints and adhesion of tendons. This triad of factors is edema, inflammation, and immobilization. (Beasley, 1981) Thus, the principles for primary care of burned hands leading to rapid recovery and minimal complications are now established and many kinds of methods are proposed. But, despite much effort, many circumstances prevent ideal primary care and we are still confronted with the need for secondary repairs. Secondary repair of burned hands requires a careful, cojmprehensive and individualized plan for each patien, and with due respect to all the patient's problems. We reviewed 42 cases of the postburn hand deformities which were operated on in our Department of Plastic and Reconstructive Surgery from 1976 to 1981.

수면무호흡증이 혈압에 미치는 영향

이희상,이성훈,조현상,남궁기,한승진,방승규 大韓神經精神醫學會 1997 신경정신의학 Vol.36 No.1

본 연구에서는 수면무호흡증이 혈압에 미치는 영향을 살펴보고자 하였다. 연구대상은 1995년 1월1일부터 1995년 10월 31일까지 코골기와 수면무호흡 증상으로 인해 연정 뇌기능 수면 연구소에 수면다원검사가 의뢰된 140명의 환자를 대상으로 하였으며 그 중에서 원발성 고혈압을 제외한 심혈관계 질환이 있거나 항고혈압제를 복용하고 있는 환자, 그리고 자료가 불충분하거나 수면무호흡증으로 진단되지 않은 24명을 제외하고 나머지 116명을 최종분석 대상으로 하였다. 이들 대상 환자들로부터 얻은 자료를 수면변수와 호흡변수로 나누어 혈압과의 관계를 Pearson 상관관계와 다중회귀분석을 통해 다음과 같은 결과를 얻었다. 1) 평균 수축기 및 확장기 혈압과 유의한 상관관계가 있는 변수는 수면무호흡의 총시간 및 총횟수, 총수면시간중 평균 혈중산소포화도, 무호흡지수, 중추성 수면무호흡의 총시간 및 횟수, 폐쇄성 수면무호흡의 총시간등 이었다. 2) 다중회귀분석 결과 평균 수축기 및 확장기 혈압에 유의한 영향을 미치는 것으로 나타난 변인은 수면무호흡의 총시간이었으며 이것이 평균 수축기 및 확장기 혈압에 미치는 영향의 도는 각각 7.9%와 6.2%였다. 이상의 결과로 볼 때, 수면무호흡증이 혈압에 미치는 영향은 적은 것으로 나타났으며 무호흡의 유형에 관계없이 수면무호흡의 총시간만이 혈압에 영향을 주는 것으로 나타났다. 그리고 중추성 수면무호흡의 경우 평균 혈중 산소포화도의 감소, 각성에 따른 교감신경계 활성도의 증가이외에도 다른 기전에 의해 혈압 상승이 나타날 가능성이 있을 것으로 생각된다. Objects : This study was conducted to investigate the effects of sleep apnea syndromes on th systemic blood pressure. Methods : The subjects were 140 patients who were consulted to Yon-jung brain function and sleep research center for polysomnography due to snoring and sleep apnea from Jan. 1st 1995 to Oct. 31st 1995. Twenty-four out of 140 were excluded due to cardiovascular diseases other than systemic hypertension, antihypertensive medication, inadequate data or diagnosis other than sleep apnea syndrome. The remaining 116 were accepted as proper subjects. Using Pearson's correlation and multiple regression analysis, we looked for the relationship between sleep and respiratory variables to systemic blood pressure. Results : The results were as follows : 1) According to the correlation analysis, statistically significant variables to mean systolic and diastolic blood pressure were the total time and total number of sleep apnea, degree of oxygen saturation of total sleep time, apnea index, total time and total number of central apnea, and the total time of obstructive apnea. 2) According to the multiple regression analysis, only the total time of sleep apnea significantly affected mean systolic and diastolic blood pressure, and the degrees of affection were 7.9% and 6.2% respectively. Conclusion : these findings showed that sleep apnea syndrome had lower influence over systemic blood pressure than expected and regardless of the type, only the total time of sleep apnea influenced systemic blood pressure. In the case of central apnea, mechanisms other than hypoxemia and the increase of sympathetic activity during arousal may make it possible to increase systemic blood pressure.

혈액종양 환자의 중심정맥 카테터에 대한 헤파린 관류와 생리식염수 관류의 효과 비교

문초희,연보라,김현진,은방희,배영이 병원간호사회 2006 임상간호연구 Vol.11 No.2

Purpose: The purpose of this study was to compare the efficacy of heparin flushing and normal saline flushing in maintaining patency of central venous catheters(CVCs), and to investigate relative risk factors of CVCs' occlusion. Method: A total of 159 patients who had been inserted with CVCs were included in the department of hemato-oncology of S hospital. The heparin group consisted of 74 patients with 10u/ml diluted heparin flushing from 23 November 2004 to 15 January 2005, and the normal saline group consisted of 85 patients with 0.9% normal saline flushing from 19 January to 31 March of 2005. Result: The heparin group was significantly different from the normal saline group in CVCs' occlusion. The normal saline group offered a much longer survival duration of CVCs' patency than the heparin group. Potential bleeding signs (PT, aPTT) had no significant differences. In multivariate analysis, 0.9% normal saline (hazard ratio 0.50), chemo port (hazard ratio 3.38), and no administration of TPN (hazard ratio 0.25) were the significant factors for occlusion of CVCs. Conclusion: For maintaining the patency of CVCs, 0.9% normal saline flushing are expected to be effective in reducing drug incompatibilities, lessening the bleeding tendency and improving cost effectiveness .

미세혈관 협심증 환자의 임상상 및 지표에 관한 연구

박상호,온영근,김현정,이태훈,길효욱,김현건,박수진,송해정,한대희,방덕원,신원용,이내희,김성구,권영주 순천향의학연구소 2003 Journal of Soonchunhyang Medical Science Vol.9 No.1

Background : Microvascular angina or syndrom X is heterogenous and encompasses different pathogenetic entities. Several studies suggest that microvascular angina have good prognosis and occurs predominantly in postmenopausal women. Lipoprotein (a), total cholesterol, LDL-cholesterol, HDL-cholesterol, triglyceride, homocystein, CRP and fibrinogen are the independent risk factor of acute coronary syndrome and are the chief underlying cause of atherosclerosis. We investigated clinical characteristics and markers in patients with microvascular angina. Methods : We studies 23 patients diagnosed with microvascular angina and 16 control group with negative treadmill exercise test and no significant coronary angiogram. We investigated clinical characteristics in patients group and evaluated symptom during a follow-up period of 4.2± 2.5 month. Exercise time in treadmill exercise test was compared between patients and control group. We assessed the level of markers, such as lipoprotein (a), total cholesterol, LDL-cholesterol, HDL-cholesterol, triglyceride, homocystein, CRP and ESR in two groups. Results : There is no difference in age between two groups. Patients group had a tendency to increase in female, hypertension, DM, and smoking history, but there was no statistical significance difference. Exercise duration time was significantly decreased in patients with microvascular angina (p<0,05). Serum lipoprotein (a), total cholesterol, LDL-cholesterol, HDL-cholestrol, Triglyceride, ESR, Fibrinogen and Homocystein value were no significant difference between microvascular angina and control group. Only CRP was significantly high in patients with microvascular angina (p<0.05). Conclusion : There was no statistical significance difference in age, sex, hypertension, DM and smoking history between two groups but patients group had a tendency to increase the prevalence in hypertension, DM and smoking history, female. Also, Symptom improvement and disappearance in follow-up was presented in 11 patients of 19(57.9%). Exercise tolerance was worse in patients group than control group. CRP value was significantly higher in patients group than control group. There was no statistical significance difference in lipoprotein (a), total cholesterol, LDL-cholesterol, HDL-cholesterol, triglyceride, homocystein, ESR and Fibrinogen between two groups.