Autophagy and KRT8/keratin 8 protect degeneration of retinal pigment epithelium under oxidative stress

Baek, Ahruem,Yoon, Soojin,Kim, Jean,Baek, Yu Mi,Park, Hanna,Lim, Daehan,Chung, Hyewon,Kim, Dong-Eun Taylor and Francis 2017 AUTOPHAGY Vol.13 No.2

<P>Contribution of autophagy and regulation of related proteins to the degeneration of retinal pigment epithelium (RPE) in age-related macular degeneration (AMD) remain unknown. We report that upregulation of KRT8 (keratin 8) as well as its phosphorylation are accompanied with autophagy and attenuated with the inhibition of autophagy in RPE cells under oxidative stress. KRT8 appears to have a dual role in RPE pathophysiology. While increased expression of KRT8 following autophagy provides a cytoprotective role in RPE, phosphorylation of KRT8 induces pathologic epithelial-mesenchymal transition (EMT) of RPE cells under oxidative stress, which is mediated by MAPK1/ERK2 (mitogen-activated protein kinase 1) and MAPK3/ERK1. Inhibition of autophagy further promotes EMT, which can be reversed by inhibition of MAPK. Thus, regulated enhancement of autophagy with concurrent increased expression of KRT8 and the inhibition of KRT8 phosphorylation serve to inhibit oxidative stress-induced EMT of RPE cells as well as to prevent cell death, suggesting that pharmacological manipulation of KRT8 upregulation through autophagy with combined inhibition of the MAPK1/3 pathway may be attractive therapeutic strategies for the treatment of AMD.</P>

تجليات الانزياح اللغوية والسردية في نماذج من قصص زكريا تامر

백혜원(Baek, Hyewon) 한국아랍어아랍문학회 2021 아랍어와 아랍문학 Vol.25 No.3

تسعى الباحثة إلى التركيز على السمات اللغوية والسردية التي تشكل الأسلوب الخاص لقصة زكريا تامر وتمنحها هوية أدبية فريدة وتكسبها أهمية نقدية وجمالية مع الاستعانة بمفهوم الانزياح (écart) علما بأن كل هذه السمات نجمت عن ظاهرة الانزياح في الخطاب الأدبي. وتعد تجليات لانزياح بحد ذاتها من الظواهر الأسلوبية الرئيسة من ناحية، ومن أهم المحددات للأسلوب وتعريفاته في العمل الأدبي من ناحية أخرى. ومن هنا يمكن القول بأن الدراسات الأسلوبية الشكلية لقصة زكريا تامر لا بد من أن تبدأ بالدرجة الأولى من تسليط الضوء على مفهوم الانزياح وفعالياته في القصص. وبناء على ذلك يتطرق البحث من باب الدراسة النظرية إلى مفهوم الانزياح بتفاصيله، وأهمية مفهوم الانزياح في دراسة الأسلوب الأدبي، ويحدد معايير ت This study “The linguistic and narrative manifestations of deviation in the stories of Zakaria Tamer” spotlights the linguistic and narrative characteristics of the stories of Zakaria Tamer focusing on the concept of deviation. These deviation-caused characteristics form a specific literary style in the stories, and ultimately gives them a unique literary identity and aesthetic importance. This research focuses on deviation because deviation is not only a stylistic characteristic of a work of literature, but is also one of the most important criteria in defining the literary style of a specific piece of literature. Therefore, it is absolutely necessary to focus on the concept of deviation and the linguistic and narrative characteristics that are derived from deviation when analyzing Zakaria Tamer’s work from the perspectives of literary style, linguistics, and structure (as opposed to the content or message).

Proving the Existence of Unity in Pre-Islamic Qasīda: The Mu‘allaqāt of Labīd as a Case Study

백혜원(Hyewon Baek) 한국중동학회 2024 한국중동학회논총 Vol.45 No.1

This research works to negate the absence of organic unity in pre-Islamic poetry and seeks to prove the existence of the unity of the poem in its structure and themes and its wrapping around one fundamental vision. This study uses concepts based on linguistics and structuralism, which are based on mutual relationships between the elements that make up both structures, based on what the researcher Suhail Al-Shamli proposed regarding the rule of harmony in his study, “On the Rules of Harmony of Pre-Islamic Poetry: Al-Mu‘allaqāt as an Example”.

Ticks Collected from Wild and Domestic Animals and Natural Habitats in the Republic of Korea

Baek-Jun Kim,Hyewon Kim,Sohyun Won,Heung-Chul Kim,Sung-Tae Chong,Terry A. Klein,Ki-Gyoung Kim,Hong-Yul Seo,Joon-Seok Chae 대한기생충학열대의학회 2014 Parasites, Hosts and Diseases Vol.52 No.3

A Role for Arabidopsis miR399f in Salt, Drought, and ABA Signaling

Baek, Dongwon,Chun, Hyun Jin,Kang, Songhwa,Shin, Gilok,Park, Su Jung,Hong, Hyewon,Kim, Chanmin,Kim, Doh Hoon,Lee, Sang Yeol,Kim, Min Chul,Yun, Dae-Jin Korean Society for Molecular and Cellular Biology 2016 Molecules and cells Vol.39 No.2

MiR399f plays a crucial role in maintaining phosphate homeostasis in Arabidopsis thaliana. Under phosphate starvation conditions, AtMYB2, which plays a role in plant salt and drought stress responses, directly regulates the expression of miR399f. In this study, we found that miR399f also participates in plant responses to abscisic acid (ABA), and to abiotic stresses including salt and drought. Salt and ABA treatment induced the expression of miR399f, as confirmed by histochemical analysis of promoter-GUS fusions. Transgenic Arabidopsis plants overexpressing miR399f (miR399f-OE) exhibited enhanced tolerance to salt stress and exogenous ABA, but hypersensitivity to drought. Our in silico analysis identified ABF3 and CSP41b as putative target genes of miR399f, and expression analysis revealed that mRNA levels of ABF3 and CSP41b decreased remarkably in miR399f-OE plants under salt stress and in response to treatment with ABA. Moreover, we showed that activation of stress-responsive gene expression in response to salt stress and ABA treatment was impaired in miR399f-OE plants. Thus, these results suggested that in addition to phosphate starvation signaling, miR399f might also modulates plant responses to salt, ABA, and drought, by regulating the expression of newly discovered target genes such as ABF3 and CSP41b.

혼합효과 랜덤 포레스트를 적용한 중학생의 학업성취도 설명 변인 탐색 : 국가수준 학업성취도 평가 결과를 중심으로

정혜원(Hyewon Chung),김아름(Arum Kim),백예은(Yeheun Baek) 한국교육평가학회 2022 교육평가연구 Vol.35 No.1

본 연구는 중학생의 국어, 수학, 영어 학업성취도의 주요 설명 변인을 탐색하고자 수행되었다. 이를 위해 한국교육과정평가원에서 제공하는 2020년 국가수준 학업성취도 평가의 중학생 자료를 활용하였으며, 학생이 학교에 내재된 다층 구조의 속성을 고려한 머신러닝 기법인 혼합효과 랜덤 포레스트(Mixed-effects random forest)기법을 적용하였다. 주요 연구 결과는 다음과 같다. 국어, 수학, 영어 공통적으로 ‘스스로 공부하는 시간’, ‘수업시간 집중도’, ‘운동 빈도’와 관련된 문항과 더불어 ‘학습 자료를 읽다가 혼돈되는 부분이 있을 때 되돌아가 이해하려고 노력한다’와 같은 학습 태도와 관련된 문항이 주요 설명 변인으로 도출되었다. 또한 교과별로 특징적으로 나타난 결과를 확인할 수 있었는데, 수학과 영어의 경우 ‘사설기관에서 제공하는 강의 참여 정도’와 관련된 문항과 각각의 교과 태도에서 교과에 대한 자신감과 끈기와 관련된 문항이 도출되었으나 국어의 경우 도출되지 않았다. 이 외에 국가수준 학업성취도 평가에서 제공하는 다양한 학생, 교사, 학교장 수준의 다양한 설문문항을 활용함으로써 도출된 분석 결과를 바탕으로 중학생의 국어, 수학, 영어 교과의 균형 있는 학업성취를 위한 시사점을 논의하였다. This study aimed to explore the variables explaining middle school students’ academic achievement in Korean, math, English based on National Assessment of Educational Achievement(NAEA). For this purpose, the Mixed-effects random forest, a machine learning technique that considers multi-level data structure was used. The main results are as follows. First, the questions related to ‘study time on my own’, ‘concentration of class time’, ‘frequency of exercise’ and the questions related to the overall learning attitude, such as ‘I try to return and understand when there is confusion while reading learning materials’ were commonly selected as important explanatory variables in Korean, math, English. On the other hand, questions related to ‘participation in lectures provided by private institutions’, ‘confidence and persistence in subjects’ were selected as explanatory variables in math and English only, but not Korean. In addition, based on the analysis results derived by using various questions at the level of students, teachers, and principals provided in the national-level academic achievement evaluation, implications for balanced academic achievement of middle school students’ Korean, math, and English were discussed.

전기자동차의 사용자 목적 수행을 위한 아웃사이드 도어 핸들 디자인 제안

송혜원(Hyewon Song),백승선(Seungseon Baek),윤원재(Wonjae Youn),한혜영(Hyeyoung Han),황다경(Dakyung Hwang),박기철(Kicheol Pak) 한국디자인학회 2024 한국디자인학회 학술발표대회 논문집 Vol.2024 No.6

중학생의 수업 이해도에 대한 성취목표 지향성과 자기결정성 동기 관계의 잠재계층 분류 및 영향요인 탐색

백승희(Baek, Seunghiey),정혜원(Chung, Hyewon) 한국교육방법학회 2017 교육방법연구 Vol.29 No.3

The purpose of this study was to investigate the heterogeneous associations of achievement goal orientation and self-determination with learning understanding as well as to test the effects of factors on determining the latent classes. In order to do this, data from the 3th round data 6,744(7th grade students 3,307 male students and 3,437 female students) of KELS 2013(Korea Education Longitudinal Study 2013) is employed. Regression mixture analysis identified four latent classes with differential associations of achievement goal orientation and self-determination with learning understanding. Multinomial logistic regression analysis showed that the gender, academic self-concept, self-management, parent-child interactionand parents’ academic involvement were significant determinants of the latent classes. But teacher and peer relationship were not significant factors in determining the classes. The findings of this study imply that the associations of achievement goal orientation and self-determination with learning understanding might differ across middle school students and various student and family factors were significantly related to the identified latent classes. Finally, Implication of the study and recommendations for future research were discussed. 본 연구는 중학교 1학년 학생들의 수업 이해도에 대한 성취목표 지향성과 자기결정성 동기 사이에서 이질적인 관계가 존재하는지 확인하고, 각 잠재계층을 결정하는 데 영향을 미치는 요인들을 탐색하는 데 목적이 있다. 이를 위하여 한국교육종단연구2013(Korea Education Longitudinal Study; KELS)의 초등학교 5학년 패널 3차년도 자료를 사용하였으며, 중학교 1학년 6,744명(남학생: 3,307명(49%), 여학생: 3,437명(51%))을 대상으로 연구를 실시하였다. 회귀혼합분석을 활용하여 성취목표 지향성 및 자기결정성 동기와 수업 이해도의 관계 양상에서 잠재계층을 확인한 결과 4개의 이질적인 계층으로 구분되었으며, 다항 로지스틱 회귀분석을 활용하여 4개의 잠재계층을 결정하는 예측요인을 알아본 결과 개인요인인 성별, 학업적 자아개념, 자기관리 가정요인인 부모자녀 상호작용, 부모의 학업지원이 계층을 구분하는 주요한 예측요인으로 도출되었다. 반면, 학교요인인 교사관계, 교우관계는 계층의분류를 유의미하게 예측하지 못했다. 본 연구는 수업 이해도에 대한 성취목표 지향성과 자기결정성동기의 이질적 관계를 검증함으로써 집단 내 변인 간 관계의 이질적 속성을 확인하고 잠재집단을 결정하는 다양한 개인과 가족 영향요인을 확인하였다는 점에서 의의가 있다. 마지막으로, 본 연구의 구체적인 시사점과 후속 연구를 위한 제언을 논의하였다

청소년들의 학교생활적응의 변화패턴과 지역사회인식의 동시 및 지연효과

백승희 ( Baek Seunghiey ),정혜원 ( Chung Hyewon ) 경북대학교 중등교육연구소 2018 중등교육연구 Vol.66 No.1

This study investigated the changing trajectory of adaptation to school life and contemporaneous and lagged effects of community awareness in Korean adolescents using KCYPS(Korean Children and Youth Panel Survey) panel data from the 1th to 6th wave. For the analysis, the latent growth model was applied to six time points(7th grade-12th grade). As the main results, First, as students advanced in school year, school life adaptation values showed signs of decrease following a preceding increasing period. Second, results of verifying the impact of the contemporaneous effects of community awareness on school life adaptation showed that 7th graders who displayed higher community awareness also displayed higher school life adaption values at the 7th grade. Consistent aspects were displayed over the 6 years of data, thus implying the existence of contemporaneous effect at concurrent periods. Third, analysis on impact of preceding community awareness on school life adaptation showed that 8th, 10th graders with higher community awareness displayed higher school life adaptation at the 9th and 12th grade, thus confirming the lagged effect. Implication of the study and recommendations for future reseach were discussed.

국가수준 학업성취도 평가 결과에 기반한 고등학생의 학업성취도 예측 변인 탐색

정혜원 ( Hyewon Chung ),김아름 ( Arum Kim ),백예은 ( Yeheun Baek ) 경북대학교 중등교육연구소 2021 중등교육연구 Vol.69 No.4

The purpose of this study is to explore the variables predicting achievement of high school students in 2020 when online classes were conducted. To do this, the mixed-effects random forest, one of machine learning techniques considering the multi-level data structure, was applied to data from 2018, 2019, and 2020 of high school students' National Assessment of Educational Achievement(NAEA). The main results are as following. In 2020 academic achievement, the questionnaire related to lecture participation provided by private institutions in ‘after-school activities’ was commonly selected for Korean, mathematics and English achievements. In both Korean and English achievements, the questionnaires related to students’ academic motivation and sense of belonging to school in ‘learning-related affective characteristics’ and ‘online class environment' were selected as important variables. In addition, the questionnaire related EBS education broadcasting viewing time in ‘after-school activities' was commonly selected for both mathematics and English. Based on the result, implications for improving high school students' academic achievement were discussed.