Recombinant canstatin inhibits angiopoietin‐1‐induced angiogenesis and lymphangiogenesis

Hwang‐,Bo, Jeon,Yoo, Ki Hyun,Park, Jong‐,Hwa,Jeong, Han‐,Sin,Chung, In Sik Wiley Subscription Services, Inc., A Wiley Company 2012 International journal of cancer: Journal internati Vol.131 No.2

<P><B>Abstract</B></P><P>We describe the effect of recombinant canstatin, the NC1 domain of the α2 chain of Type IV collagen, on suppression of angiogenesis and lymphangiogenesis both <I>in vitro</I> and <I>in vivo</I>. Recombinant canstatin produced from stably transformed <I>Drosophila</I> S2 cells reduced the expression of angiopoietin‐1 in hypoxia mimetic agent, CoCl<SUB>2</SUB>‐treated CT‐26 cells. Recombinant canstatin inhibited proliferation, tube formation and migration of human angiopoietin‐1 (rhAngpt‐1)‐treated human umbilical vein endothelial cells (HUVEC) and lymphatic endothelial cells (LEC). Recombinant canstatin suppressed the expression of Tie‐2 and vascular endothelial growth factor‐3 (VEGFR‐3) transcripts in rhAngpt‐1‐treated HUVEC and LEC, respectively. The inhibitory effect of recombinant canstatin on tumor growth was also investigated using a heterotopic CT‐26 colon carcinoma animal (BALB/c mice) model. Recombinant canstatin reduced the final volume and weight of tumors, and blood and lymphatic vessel densities of tumors, which were evaluated by CD‐31 and LYVE‐1 immunostaining. Immunohistochemical analysis showed that recombinant canstatin dramatically reduced the expression of angiopoietin‐1 in CT‐26 colon carcinoma‐induced tumor, but not the expression of VEGF‐C. Tie‐2 and VEGFR‐3 expressions were also reduced in recombinant canstatin‐treated tumors. These results indicate that recombinant canstatin has anti‐tumoral activities against CT‐26 colon carcinoma cells. Recombinant canstatin reduces the expression of angiopoietin‐1 in hypoxia‐induced CT‐26 cells and inhibits the angiogenic and lymphangiogenic signaling induced by angiopoietin‐1. Recombinant canstatin probably inhibits angiogenesis and lymphangiogenesis <I>via</I> suppression of the integrin‐dependent FAK signaling induced by angiopoietin‐1/Tie‐2 and/or VEGFR‐3.</P>

푸쉬코 메카니즘 언어학 : 원리,방법,적용에 관한 시론 le principe, la me'thode et des applications

황보순 慶北大學校 語學硏究所 1989 語文硏究 Vol.14 No.-

부산지역 산성강우의 장기적인 화학적 성상의 변화

황성욱,전보경,김찬훈,서정민,최금찬 동아대학교 환경문제연구소 2001 硏究報告 Vol.24 No.1

It is important to know the impacts of acid rain because of soil acidification, reduced biological productivity. Many researchers were interested in regional acid problems, and former research were focued in short-term trends of acid rain. It is not easy to evaluate acid rain problems because of many parameters relating to meteorological factors, which are wind, temperature, humidity and other factors. Rainfall sampling for this study was done from September 1933 to December 2000 in Dong-A University, Busan. All Samples were collected in separately to early fraction and succeeding fraction. Also, the samples were measured for pH, and EC, cations and anions were analysed by ion Chromatography.

Tough-Movement

黃保根 대구대학교 인문과학연구소 1982 人文科學硏究 Vol.1 No.-

Tough-Movement, as a transformational rule, clearly exists in English. It applies to limited construction which have as predicates adjectives like hard, tough, easy, difficult, etc. and nouns including breeze, cinch, snap and a number of more obscene expressions. The operation of it is that this rule extracts a nonsubject NP out of an extraposed complement and moves it into the position of the higher clause. Three different hypotheses are proposed for deriving sentences like "Tony is difficult for me to persuade." The Phrase Structure Hypothesis is inadequate as a linguistic theory because it fails to capture the linguistic intuition of a native speaker, and violates the principle of economy. The Coreferential NP Deletion Hypothesis also fails to explain the difference in meaning of the surface structure derived from the same underlying one. Its inadequacy is proved by the fact that the subject NP of the higher clause does not exist within the underlying structure but is derived from the application of a transformational rule. The raising Hypothesis turus out to be the best as a linguistic theory, since it not only captures the linguistic intuition of a native speaker but also explains the problems the former two can not. Tough-Movement is subject to many restrictions, selectional and syntactic. This rule operates within the domain of Postal's Cross-over Constraints and Ross's Pied Piping Convention.

고대영어의 수동구조

黃保根 대구대학교 인문과학연구소 1986 人文科學硏究 Vol.4 No.-

A structural description of passive constructions in Old English is the primary purpose of this paper. In other words, it can fairy be said to be the preliminary stage for further studies on syntactic changes in English, since diachrony must be studied on the basis of patterned synchronic data. The structural description of passives in Old English, unlike that in Modern English, is not an easy task. Major obstacles are: passives in OE have few corresponding actives, and the analysis performed only on literature-written language-not on spoken one. However, the following can be classified, as the three major patterns of OE passive constructions, by the analysis of them in Anglo-Saxon Chronicles, Alfred’s Pastoral Care, and several primary OE texts available: 1. NP hatan NP beon/wesan NP 2. NP{ weorpan }+PP (Prep) {AP} (Prep+NP) beon/wesan 3. NP weorpan +PP NP (Prep +NP) Except for the Tppe 1, the passive auxiliaries used in OE are beon, wesan and weorpan, and their semantic functions are different in the early OE period. Later they came to be used interchangibly as the original meaning of weorpan disappeared. The identification of the semantic functions of prepositions in agentive adjuncts is grueling. The reason is the agentive adjuncts can be viewed as ‘principle agents’, ‘medium’, ‘means’, ‘cause’, ‘reason’, or ‘instrumentality’according to modern interpretation. The preposition used before agent NPs in Oe are : at, by/be, fram/from, mid, of, purh, etc.

고대영어의 관계사 (Ⅰ)

황보근 대구대학교 외국어교육연구소 1986 외국어교육연구 Vol.1 No.-

백서의 정맥혈전 모델에서 장기간의 혈전노출이 정맥변 변화에 미치는 영향

황종성,권우형,박영도,서보양,권굉보 대한혈관외과학회 2004 Vascular Specialist International Vol.20 No.1

"요구하기" 사회적 언어중재가 언어장애 유아의 사회적 언어 자발 표현에 미치는 효과

황보명,강수균 한국언어치료학회 1997 言語治療硏究 Vol.6 No.1

The purpose of this study is to investigate how Social Language Intervention affect the spontaneous social language of language disordered children in the language tharapy room. For this purpose, three preschool children with language disorder were selected in the integrated preschool and nonintegrated preschool settings. Social Language Intervention of this study was limited to 'Request' among the categories of the pragmatic funtion of the social language. Using multiple baseline design accross subjects, the frequency of the spontaneou s 'Reguest' in response to the examiner's mand was measured. The frequency of the spontaneous 'Request' during free-play in the integrated and nonintegrated preschool settings was analyzed to see generalization of newly acquired social language. The results obtained from the present study are as follows: Firstly, the frequency of the spontaneous social language was increased through Social Language Intervention for children both in integrated and nonintegrated set tings. However, the generalization effect occurred significantly more often in children treated in the integrated setting. Secondly, Social Language Intervention increased the frequency of the spontaneous speech in 'Suggestion' and 'Action Request' among the subcategories of ' Request.' The other subcategories(i.e., 'Question' and 'Approval Request') stayed almost the same. In addition, children treated in the integrated setting showed better improvement in terms of frequency of spontaneous 'Suggestion' and 'Action Request.' Thirdly, Social Language Intervention increased the frequency of the spontaneou s 'Assertion' social language in children treated in the integrated setting than nonintegrated setting. Finally, Social Language Intervention increased the frequency of the spontaneou s 'Response' in children treated in the integrated setting than nonintegrated setting.

a-TN-LCD의 전기광학특성에 표면액정배향이 미치는 영향

황율연,이창훈,서대식,이보호 崇實大學校 生産技術硏究所 1996 論文集 Vol.26 No.2

The characteristics of the response time and the viewing angle on twisted nematic(TN)-liquid crystal display(LCD) and amorphous (a)-TN-LCD without rubbing were investigated. To measure the Tansmission-Voltage, response time, and viewing angle characteristics, we prepared three kinds of LC cells and then studied the surface liquid crystal alignment effect. It was found that the response time on a-TN-LCD was fast compared to the TN-LCD, and the weak anchoring strength was attributed to the fast response time on a-TN-LCD. Also, we obtained the wide viewing angle characteristics on-a-LCD. We suggest that the liquid crystal alignment effect on LCD device is very important for electro-optical characteristics.

펄스정전응력법(PEA)을 이용한 고분자 재료애 따른 공간전하분포와 전도전류 특성

황보승 호남대학교 1999 호남대학교 학술논문집 Vol.20 No.2

The purpose of this paper is to evaluate the effects of space charge on electrical characteristics in polymer insulators, which has been studied by many researchers in the world recently. In this paper, we have manufactured new measurement system which measured simultaneously both space charge distribution and conduction current. By using this system, we have analyzed the effects of space charge on conduction characteristics of polymer insulators such as XLPE and WTR-XLPE under DC voltage. We could find that the characteristics of polymer insulators have good correlation with space charge distribution in polymer insulators.