노인의 Helicobacter pylori 재균율

박주상,홍원선,이영수,이은주,명승재,정훈용,양석균,김진호,민영일 대한노인병학회 2000 Annals of geriatric medicine and research Vol.4 No.3

위암세포주에서 Recombinant Human Interferon-r와 Adriamycin의 투여순서가 항암효과에 미치는 영향

홍원선,손영숙,김창민,강윤구,이춘택,김유철,임영혁,남현석,이진오,강태웅 大韓免疫學會 1993 大韓免疫學會誌 Vol.15 No.-

Numerous previous studies, both in vitro and in vivo, have demonstrated that the cytotoxicity can be enhanced by the combination of chemotherapeutic agent and interferons(IFNs) in various types of cancer cells. We have previously reported that combined treatment of MKN-45, human gastric adenocarcinoma cells, with adriamycin(ADM) and recombinant human interferon-r(rh-IFN-r) increased in the cytotoxicity. In this study, the effects of combination timing of rh-IFN-r and ADM on the cytotoxicity against MKN-45 were investigated using MTT assay. MKN-45 was treated with rh-IFN-r and ADM in vitro on three schedules : Treat A ; rh-IFN-r and ADM were treated simultaneously, Treat B ; rh-IFN-r was treated 24 hours after the treatment with ADM, Treat C ; rh-IFN-r was treated for 72 hours and followed by the treatment with ADM. The survival of MKN -45 was inhibited by ADM dose-dependently. 102 and 103U/ml of rh-IFN-r significantly inhibited the survival of MKN-45(% survival : 35.1 ±-1.2% and 34.4 ±1.1% in Treat A and 42.5 ± 2.1% and 45.9-±2.5% in Treat C, respectively). However no difference in the survival was observed between 102 and 103U/ml of rh-IFN-r. Combined treatment with rh-IFN-r and ADM significantly augmented the cytotoxicity at low concentrations of ADM. Combined effects of rh-IFN-r and ADM were evaluated using IC30(,ag/ml) to ADM. IC30s of MKN-45 in Treat A, B and C at 102 U/ml of rh -IFN-r _ were 0.019 -?- 0.003, 0.045 :I:0.001 and 0.054 ± 0.012, respectively, while IC30 of MKN-45 treated with ADM alone was 0.052±0.004. IC30s of MKN-45 in ADM alone group, Treat A, Treat B and Treat C at 103U/ml of rh-IFN-r were 0.047 ±0.003, 0.004 -±0.001, 0.031 ±0.004 and 0.056 0.008, respectively. These results indicate IC30s of Treat A and B were significantly lower than those of ADM alone(p<0.05) and IC30s of Treat A was significantly lower than those of Treat B(p <0.01). IC30s of Treat C, however, were not different from those of ADM alone. From these results demonstrating that cytotoxic effects were increased by the combination of rh-IFN-r and ADM in the order, Treat A > Treat B> Treat C, it can be concluded that the simultaneous administration of rh-IFN-r and ADM may be the most effective method to combine these two therapeutic modalties.

간호사의 투약안전 역량 강화를 위한 교육요구 분석

양혜영, 이광희, 홍선영, 이정은 신한대학교 2021 신한대학교 논문집 Vol.44 No.-

The purpose of this study is to present basic data necessary for the development of educational programs to improve nurses' medication safety competency by analyzing educational needs. A survey was conducted on 37 clinical nurses at secondary hospitals or higher in Seoul and Gyeonggi-do. As a result of analyzing the nurse's medication experience, there were no cases of administering expired drugs, but various medication errors occurred. In addition, nurses had many organizational problems in performing oral prescriptions rather than receiving regular instructions for medication. There were six items that showed the largest statistical difference between the group of important and implementation levels of nurse's medication safety competency. In addition, as a result of the Borich’s demand analysis, the highest priority items were "Measures can be established to prevent recurrence in the event of drug side effects" and "Cases can be analyzed to find the root cause of medication errors." Based on the results of this study, follow-up studies on the development of educational programs that can strengthen nurses' medication competency should be conducted.

진행 위암환자에서 Lymphokine-Activated Killer (LAK) 활성의 저하

홍원선,김영환,송재관,강윤구,이진오,강태웅,김정룡 대한내과학회 1990 대한내과학회지 Vol.38 No.3

Natural killer(NK) and lymphokine-activated killer(LAK) activities were determined in 31 patients with unresectable stomach cancer before and immediately after chemotherapy with 5-fluorouracil, adriamycin and mitomycin C(FAM) and 31 healthy volunteers. The major purposes of the study were focused on whether peripheral blood lymphocytes(PBL) in stomach cancer patients had a similar ability in the generation of LAK activity to those in healthy volunteers and what the effect was of chemotherapy with FAM on the generation of LAK activity. LAK cells were generated in vitro by culturing human PBL with 100 U/㎖ of recombinant human interleukin-2(rH-IL-2) for 72 hours. K562(human myelogenous leukemia cell line) and MKN-45(human stomach adenocarcinoma cell line) were used as target cells for NK and LAK activities. NK activity against K562, a sensitive line, was significantly depressed in patients with stomach cancer compared with that in healthy volunteers(p<0.01). However, LAK activity against K562 was similar to that of the controls. Age, sex and performance status(ECOG 0-2 and 3-4) did not have an influence on both NK and LAK activities. LAK activity was significantly higher than NK activity, either against K562 or MKN-45, in both healthy volunteers and patients with stomach cancer(p<0.001). NK and LAK activities against MKN-45, a NK-resistant line, in patients with stomach cancer showed significantly lower levels than those in healthy volunteers. We also analyzed the effect of chemotherapy with FAM on NK and LAK activities, with no suppression of NK and LAK activities being observed. In this study, we have demonstrated that PBL of stomach cancer have a reduced ability to generate LAK activity in response to rH-IL-2. However, LAK activity generated from PBL receiving chemotherapy with FAM was similar to that of PBL without chemotherapy.

사람폐암세포주 (PC-14)에서 Cyclosporin A에 의한 Adriamycin 내성의 극복

김영환,홍원선,송재관,강윤구,이진오,강태웅,김건열,한용철 대한내과학회 1990 대한내과학회지 Vol.38 No.3

Cyclosporin A and verapamil were tested using MTT assay to evalute the modification effect on the resistance to adriamycin in a human lung cancer cell line(PC-14) and its resistant subline(PC-14/A). PC-14/A was derived by the continuous exposure of PC-14 to incremental concentrations of adriamycin. PC-14/A was 2.5 times more resistant to adriamycin in terms of ICso than PC-14. Cyclosporin A alone, at a concentration of 2.5㎍/㎖, inhibited the growth of PC-14 to 68.3%. 2.5㎍/ ㎖ and 5.0㎍/㎖ of cyclosporin A showed an increase in the cytotoxicity of adriamycin (p<0.01) with 5.0㎍/㎖ being greater than 2.5㎍/㎖(p<0.01). Excluding the direct cytotoxic effect, however, cyclosporin A did not increase in the sensitivity of PC-14 to adriamycin but only showed an additional cytotoxic effect with adriamycin. Verapamil (up to 6.0㎍/㎖) did not inhibit the growth of PC-14. 3.0㎍/㎖ of verapamil did not increase the cytotoxic effect of adriamycin. The combination of cyclosporin A and verapamil with adriamycin enhanced the cytotoxicity of adriamycin, but the result was similar to that of cyclosporin A with adriamycin. 5.0㎍/㎖ of cyclosporin A modified the adriamycin resistance of PC-14/A(SR, 3.2). However, 3.0㎍/㎖ of verapamil did not significantly reverse the adriamycin resistance of PC-14/A. The modified effect of the combination of 5.0㎍/㎖ of cyclosporin A and 3.0㎍/㎖ of verapamil was similar to that of 5.0㎍/㎖ of cyclosporin A alone in PC-14/A. These results demonstrate that cyclosporin A has an additional cytotoxic effect with adriamycin in PC-14 and PC-14/A and has overcome the acquired resistance to adriamycin in PC-14/A. They also suggest that cyclospoin A may have the therapeutic potential in the treatment of human lung cancer.

조선대학병원에서 경험한 삼일열 말라리아의 임상적 고찰

서영선,김양수,김태균,박유환,홍순표,정춘해 조선대학교 부설 의학연구소 1999 The Medical Journal of Chosun University Vol.24 No.1

In the five years since 1993 Malaria has made a ceaseless attack on the native Koreans as an indigenously formed disease in addition to the imported one. This study was aimed at clinical symptoms and signs of Malaria which had occurred indigenously in Cheon-Nam. 18 cases were reviewed retrospectively in this study which was composed of 17 men and 1 woman. They were admitted to Chosun Hospital because of the indigenous Malaria in 1998. Fever and chill , the most frequent symptoms in early stages made a 100% occurrence followed by general myalgia(89%) and headache(83%). Physical examination of those admitted showed the splenomegaly most frequently in 61% of the patients. It took four to ten days to visit the hospital since the symptoms first occurred in the patients. Gametocytes among the Malarial life cycle were observed most commonly (91.3%) in the occurrence of the disease and the prestages of the life cycle as well. The hematologic abnormalities observed was Anemia(89%), Thrombocytopenia(61%), and Neutropenia(50%) in the order named. On the third hospital day since giving the standard Malarial drugs parasitemia decreased remarkably (1540/㎕ : Mean parasitic concentration) and 28th day of treatment no parasitemia were found. In conclusion the patients suffering from malaria in Cheon-Nam had been in an armistice area and the symptoms were so relatively mild that the responses to treatment were good and relapses occurred. But, it requires further epidemiologic survey and more concern about the indigenous Malaria.

말기환자 관리에 대한 의사들의 태도

최윤선,김장욱,신승욱,이영미,이태호,홍명호 가톨릭대학교 가톨릭대학교 간호대학 호스피스 교육연구소 1998 호스피스논집 Vol.3 No.-

Background : Nowadays, it is possible to prolong the life. And to improve the quality of life of the dying patients, they need to receive not only the physical, pscychological, social and spiritual care but also comprehensive and continuous care. Also, doctors have to consider many ethical issues especially in the dying patients. The purpose of this study is to analyze the doctors' attitude of those ethical issues and emphasize the importance of hospice. Methods : During September 1996, doctors who worked for a university hospital participated in this study by responding to the pre-made questionnaire. It dealt with the most suffering pain, the preferred death place, the use of life maintaining equipments, the application of cardiopulmonary resuscitation(CPR) to terminal patients and the doctors' experience and attitude euthanasia. Results : ① The most suffering symptom in dying patients was pain(60.0%). ② The death place was recommended by doctors was home(58.8%) and hospital (15.4%) in order. ③ 56.9% of doctors answered 'the life maintaining equipments isn't needed', 29.2% of doctors answered' according to the case', and 'needed' was 12.3%. ④ The application of CPR to the terminal patients, without the DNR chart, the only 15.4% of doctors answered "Do not CPR." But with the DNR chart, the percents of 'Do not CPR' was increased to 69.2%. The resident group did more CPR than specialist group(p<0.01). ⑤ The 50.8% of doctors agreed with the passive euthanasia. Conclusions : Many doctors follow the family member's opinion in the ethical issues of the dying patients. Euthanasia is forbidden by the law in Korea, but many doctors agree with the passive euthanasia. Hospice can solve the debating ethical issues including the euthanasia.

연 X-선 현미경을 이용한 금 나노입자 세포영상

권영만,김한경,김경우,김선희,윤홍화,천권수,강성훈,박성훈,정선관,윤권하 韓國電子顯微鏡學會 2008 Applied microscopy Vol.38 No.3

연 x-선 현미경은 ‘물의 창’ 영역(2.3~4.4 nm)의 파장을 이용하여, 수십 nm의 분해능으로 세포를 파괴하지 않고 살아있는 상태에서 세포의 내부구조를 관찰할 수 있어 가시광선현미경과 전자현미경을 단점을 보완하는 특징을 갖는 세포 생물학 연구에 적합한 현미경이다. 그러나 기존 연 x-선 현미경은 광원으로 방사선 가속기를 이용하기 때문에 사용이 제한적이었다. 이에, 본 연구에서는 2.88 nm의 연 x-선을 광원으로 사용하는 소형 연 x-선 현미경을 이용하여, 내포작용에 의해 금 나노입자를 포획한 HT1080과 MDA-MB 231 세포의 영상을 약 60 nm 분해능으로 획득하였다. 금 나노입자의 세포에 대한 독성을 제거하기 위하여 폴리에틸렌 글리콜을 캡핑하였고, 2.88 nm 파장의 연 x-선에 대하여 충분한 조영효과로 인하여 세포영상에서 뚜렷한 대조도를 나타내었다. 내포작용에 의해 액포에 포함되어 있는 다양한 크기의 금 나노입자 군집을 확인하였으며, 세포내부의 액포의 분포상태도 관찰할 수 있었다. 따라서 고분해능을 가진 소형 연 x-선 현미경을 이용하여 금 나노입자를 세포내의 미세기관이나 특정 단백질에 표지하면 연 x-선에 대한 조영효과의 증가에 의하여 더욱 유용한 정보를 획득할 수 있을 것으로 생각한다. A compact soft x-ray microscope operated in the ‘water window’ wavelength region (2.3~4.4 nm) was used for observing cells with nano-scale spatial resolution. To obtain cellular imaging captured with colloidal gold nanoparticles using a compact soft x-ray microscope. The colloidal gold nanoparticles showed higher contrast and lower transmission more than 7 times than that of cellular protein on the soft x-ray wavelength region. The structure and thickness of the cell membrane of the Coscinodiscus oculoides (diatome) and red blood cells were seen clearly. The gold nanoparticles within the HT1080 and MDA-MB 231 cells were seen clearly on the soft x-ray microscopy. The gold nanoparticles were aggregated within vesicles by endocytosis.

Permanent waVe와 Decoloration에 의한 모발손상에 관한 연구

배선향,윤수홍,이도영 한국위생과학회 2002 한국위생과학회지 Vol.8 No.2

펌 및 탈색 시 모발 손상을 줄이기 위한 기초자료를 제공하기 위해 20대 여대생의 건강한 모발을 펌 및 탈색을 하면서 전자 현미경으로 그 형태적 변화를 조사한 결과는 다음과 같았다. 자연 모발은 굵기가 60~80 ㎛인 비교적 가는 건강한 직모로 나타났다. 모표피(hair cuticle)의 비늘은 벌어지거나 벗겨짐이 서로 밀착되어 있었으며, 비늘의 겹쳐진 폭이 규칙적이었다. 또한 비늘 사이의 경계가 뚜렷하였고, 비늘의 표면은 매끈하였다. 25 ℃에서 1회 펌을 한 모발의 경우 한 부위에서만 비늘의 가장자리가 벌어졌을 뿐 다른 부위에서는 비늘의 벌어짐이 없이 밀착된 상태로 나타났다. 25 ℃에서 3회 펌을 한 모발의 경우에는 네 부위에서 비늘의 가장자리가 약간 더 벌어졌으나 25 ℃에서 1회 펌을 한 모방의 손상 정도와 큰 차이가 없었다. 50 ℃에서 1회 펌을 한 모발은 전체부위에서 비늘이 약간씩 벌어졌다. 25 ℃에서 3회 펌을 한 모발에 비해 비늘의 형태가 더 손상된 형태로 나타났다. 50 ℃에서 3회 펌을 시술한 모발은 심하게 손상되었다. 약 40 %의 비늘 가장자리가 벌어졌고, 약 10 %의 비늘은 미세한 주름이 생겼으며, 모발의 한 부위에는 구멍이 생겼다. 25 ℃에서 1회 탈색한 모발의 경우 전체에서 비늘이 벌어졌으며, 두 부위에서는 비늘이 탈락되어 비늘의 간격이 불규칙하였고, 전체 모발 중에서 약 15 %의 비늘에 세로 주름이 나타났다. 25 ℃에서 3회 탈색한 경우에는 모발 전체에서 비늘이 용해되어 두꺼워졌고, 모표피의 문리가 일정하지 않았으며, 모발의 몇몇 부위에 구멍이 생겼다. 50 ℃에서 1회 탈색한 경우 90 % 이상의 비늘이 벌어졌고, 약 25 %의 비늘에 뚜렷한 세로 주름이 생겼으며, 한 장의 비늘에 작은 구멍이 나타났다. 50 ℃에서 3회 탈색한 경우에는 모발 전체에서 모표피의 문리가 없어졌고, 용해된 비늘이 뭉쳐 있었으며, 또한 작은 구멍들이 여러 부위에 생겼다. 펌을 한 경우보다 탈색한 경우 모방의 손상 정도가 큰 것으로 나타났으며, 가장 손상의 정도가 큰 것은 50 ℃에서 3회 탈색한 경우로 나타났다. The morphological changes were investigated for the purpose of suggest the basic information to lesson hair damages by perm and decoloration. Women hairs were permed and decolored one time and three times at different temperature(25 ℃, 50 ℃), respectively. Then, the hairs were photographed with scanning electron microscope. The virgin hairs, which were not permed and not decolored, were morphologically healthy. Hair cuticle scales were not splayed and overlapped regularly. Perm hairs, which were treated one time at 25 ℃, were relatively healthy though one scale of hair cuticle was splayed. and, on the occasion of perm hairs treated three times at 25 ℃, four scales of hair cuticle were splayed. Hairs, which were permed at 50 ℃, were more damaged than those which permed at 25 ℃. Hair scales, which were permed at 50 ℃, were almost splayed. On the occasion of perm hairs treated three times at 50 ℃, 10 percentages of hair scales were wrinkled, and one scale was holed. Hair scales, which were decolored one time at 25 ℃ were almost splayed, and 15 percentages of hair scales were wrinkled. Hair scales, which were decolored three times at 25 ℃, were almost melted and thickened. 90 percentages of hair scales, which were decolored one time at 50 ℃, were splayed, and 25 percentages of hair scales were wrinkled. On the occasion of decoloration hairs treated three times at 50 ℃, the boundaries between scales were almost disappeared, and hair scales were melted and were agglomerated. Hairs, which were decolored, were more morphologically damaged than those which premed.

Permanent wave와 Bleaching에 의한 모발손상에 관한 연구

배선향,윤수홍,이도영 한국위생과학회 2002 한국위생과학회지 Vol.8 No.2

This study investigated the changes of hair cuticle shape by means of scanning Electron Microscope(SEM) after permanent waving and bleaching, according to different types of chemicals(perm, bleach)and of temperatures(25℃, 50℃). The subjects were Korean students in their twenties who did not go on a diet, took no drugs, and had virgin hair. Virgin hair remained dense without being splayed off the scale, overlapped in a regular basis, and clear of the boundary between scales. But, the scale of hair cuticle had been splayed, separated, and lost by permanent waving and bleaching with hair chemicals. The hair cuticle that was permanent waved at 25℃ once and three times showed almost virgin hair, and the cuticle of hair permanent waved at 50℃ once was just a little splayed, so it seemed that perm treatment frequency and the permanent wave lotion with low pH were not major factors at low temperature. However, repeating the performance of bleaching, though at low temperature, was more major harmful factor than high temperature. This result reveals that a chemical of pH 11.0 for bleach damages hair severely leaving splayed and cracked in the whole hair, and maker\s column wrinkled scales regardless of temperature.