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S-663 Highly Efficient Colorimetric Allergy Detection Based on Hierarchically-structured Nanozymes
( Jung Woo Shim ),( Sang Min Lee ),( Ho Yun Shin ),( Seongyeon Cho ),( Yiel Hea Seo ),( Yong Kyun Cho ),( Moon Il Kim ),( Sang Pyo Lee ) 대한내과학회 2016 대한내과학회 추계학술대회 Vol.2016 No.1
Nanozymes, nanomaterials with enzyme-like characteristics, have recently attracted a significant attention due to their potentials to overcome the intrinsic limitations of natural enzymes, such as low stability in harsh conditions (temperature and pH) and relatively high costs for preparation, purification, and storage. In this study, we report a highly efficient colorimetric allergy detection system by employing hierarchically-structured platinum nanoparticles (H-Pt NPs) as peroxidase mimetics. H-Pt NPs were conjugated to an antibody for detecting immunoglobulin E (IgE) analytes, which are the representative markers to diagnose allergy, and successfully integrated into the conventionally used allergy diagnostics, ImmunoCAP diagnostic test (Phadia, Uppsala, Sweden). Using this strategy, total and specific IgE were detected in a 10 min time period at room temperature with high specificity and sensitivity. The high catalytic activity and stability could allow the H-Pt NPs to replace conventional peroxidase-based immunoassay systems as part of new, rapid, robust, and convenient assay systems which can be widely utilized for the identification of clinically important target molecules.
유전자 알고리즘을 이용한 광상호연결망에서 파장분할다중방식의 리스트 스케쥴링
정혜진(Jung Hea Jin),위규범(Wee Kyun Bum),예흥진(Yeh Hong Jin),홍만표(Hong Man Pyo),변광준(Byeon Kwnag June) 한국정보처리학회 1998 정보처리학회논문지 Vol.5 No.9
The problem of scheduling general packet traffic on WDM optical interconnection networks is NP-complete. It is known that the list scheduling is a good approximation algorithm for this problem. The resulting list schedules vary depending on the order of transmitters considered to be placed on each time slot. We propose an improvement of the list scheduling that tries different orders of transmitters to obtain shorter schedule lengths. Genetic algorithms are used to explore various orders of transmitters.
넙치 ( Paralichthys olivaceus ) 의 in vitro 난모세포 성숙과 배란에 미치는 TBT , TPhT 및 Aroclor 1254 의 영향
백혜자(Hea Ja Baek),정지현(Jee Hyun Jung),전중균(Joong Kyun Jeon) 한국수산과학회 2001 한국수산과학회지 Vol.34 No.6
성숙 시기의 넙치 (Paralichthys olivaceus) 어미를 대상으로 난모세포의 GVBD와 배란유도과정에 TBT/TPhT와 Aroclor 1254의 저해효과를 조사한 결과, TBT (또는 TPhT)와 Aroclor 1254 모두 GVBD 과정과 HCG로 전처리한 배란유도 과정에 저해효과를 보였으며, 난모세포의 반응은 Aroclor 1254보다는 TBT에 좀 더 민감한 것으로 나타났다. TBT의 처리 농도별 차이는 TBT 0.1과 1 ppm에서 가장 낮은 GVBD 유도율을 보였으나 0.0001∼1 ppm 사이에 유의한 차이는 관찰할 수 없었다. TBT의 배란저해 효과는 HCG 처리구에 비해 HCG+TBT의 모든 실험구 (0.01, 0.1, 1 ppm)에서 뚜렷하게 나타났으며, TBT 처리구 중 가장 높은 농도인 HCG+TBT 1 ppm에서 가장 낮은 배란율을 보였다. 넙치의 GVBD와 배란과정을 유기주석화합물 (TBT, TPhT)이 방해함으로써 이 시기의 주요 호르몬인 progestogens 작용이 저해 되는 것으로 생각되며, 앞으로 난소발달 단계별로 세분화하여 그 저해 작용 메커니즘에 대한 실험이 요구된다. The effects of tributyltin (TBT), triphenyltin (TPhT) and Aroclor 1254 on germinal vesicle breakdown (GVBD) and ovulation of olive flounder (Paralichthys olivaceus) were investigated in in vitro bioassay. TBT, TPhT and Aroclor 1254 showed the inhibition effects on GVBD and ovulation in response to HCG. The oocyte response appeared to be more sensitive to TBT than Aroclor 1254. TBT was more effective in inhibiting GVBD at concentrations of 0.1 and 1 ppm. However, no significant inhibition was observed in concentrations tested (0.0001∼1 ppm). Significant inhibition of ovulation in response to HCG occurred at TBT (0.01, 0.1, 1 ppm), TPhT (0.01, 0.1, 1 ppm) and Aroclor 1254 (0.01, 1 ppm, except 0.1 ppm), compared to HCG control. The lowest ovulation rate was measured at 1 ppm TBT. These data suggest that TBT (or TPhT) could possibly interfere the actions of progestogens to induce GVBD and ovulation in in vitro bioassay system.
Lee, Hyong-Joo,Lee, Jung-Min,Park, Jae-Yeon,Lee, Jong-Hoon,Kim, Jeong-Hwon,Chang, Hea-Choon,Chung, Dae-Kyun,Kim, Somi-Cho The Microbiological Society of Korea 2002 The journal of microbiology Vol.40 No.2
The whole gal/lae operon genes of Lactococcus lactis ssp. lactis 7962 were reported as follows: galA-galM-galK-galT-lacA -lacZ-galE. The galK gene encoding a galactokinase involved in one of the Leloir pathways for galactose metabolism was found to be 1,197 bp in length and encodes a protein of 43,822 Da calculated molecular mass. The deduced amino acid sequence showed over 50% homology with GaIK proteins from several other lactic acid bacteria. The galK gene was expressed in E. coli and the product was identified as a 43 kDa protein which corresponds to the estimated size from the DNA sequence. The galactokinase activity of recombinant 5. coli was about 8 times greater against that of the host strain and more than 3 times higher than the induced L. lactis 7962.