Cortisone acetate 投與에 依한 Pneumocystis carinii 肺炎의 發症 課程

閔庚璇,李英河,羅榮彦,辛大煥 충남대학교 의과대학 지역사회의학연구소 1990 충남의대잡지 Vol.17 No.2

This study was undertaken to determine the provocative characteristics of experimental Pneumocystis carinii pneumonia in cortisone acetate treated rats. A Total of 55 male Sprague-Dawley rats, body weights 166-224g, were used. Five out of them were used as control group and remaining 50 were as experimental groups. In experimental groups, the rats were injected with 25mg of cortisone acetate twice a week for 10 weeks subcutaneously and drinking water contained 1000mg tetracycline hydrochloride per liter. Control group was not administered any drug. The 5 rats were sacrified weekly at each week in experimental group. but control group was sacrified at 10 weeks. For arterial blood gas analysis, blood was collected from carotid artery of rats using catheter. The rat's lung were removed after killed. The stamp smeared specimens of lungs were prepared and stained toluldin blue 0 for the quantification of P.carinii cysts. For the histopathologic observations, lungs were fixed in 10% buffered neutral formalin, cut into sections and stained with hematoxylin-eosin stain. For the histologic score of the intensity of P.carinii pneumonia, the sections were stained with Gomori's methenamine silver stain. The results obtained were as follows : 1. In the arterial blood gas analysis of cortisone acetate treated rats, blood pH and PaCO_2 was showed normal range. But PaO_2 was slightly decreased in experimental period. 2. Histopathologic finding of H-E staining sections were found in order of infiltration of lymphocyte and alveolar macrophage, eosinophilic foamy exudate, thickening of alveolar septum, desquamation of pneumocyte and fibrinoid degeneration and so on, but plasma cell was not found. 3. The histologic score of the intensity of P.carinii pneumonia was increased in proportion to the cortisonized periods of rats. 4. The number of cysts in the stamp smeared specimens of lungs were increased in proportion to the cortisonized periods of rats. And highly significant correlation was found between the histologic score of the intensity of P.carinii pneumonia and the number of cysts countered in the stamp smeared specimens of lungs(r=0.8620, ANOVA test p<0.0001). The present study revealed that provocative characteristics of experimental P. carinii pneumonia was highly significant relationship with the histologic score of P. carinii pneumonia and the number of cysts in the stamp smeared specimens of lung.

효율적인 부부의사소통 교육프로그램 개발을 위한 기초연구

박은주,김경신 全南大學校家政科學硏究所 1996 生活科學硏究 Vol.6 No.-

The purpose of this study was to provide the basis for the development of an education program for effective marital communication. To develop the program, several issues in marital relationships must be identified and unique aspects of education should be examined. An effective marital communication program was showed, that was consisted of the understanding method of spouse and effective communication skills between husband and wife. Also this program suggested characteristics of marital relationships and some practical implications. To develop the education programs for effective marital communication, further studies should be done in systematic theory and practice.

음성 인식 헬스케어 시스템

신진우,조우승,조성빈,김재훈,정지은,사예지,백경동,박동규 순천향대학교 부설 산업기술연구소 2021 순천향 산업기술연구소논문집 Vol.27 No.2

As modern people's interest in health grows, wearable healthcare-related technologies that can collect biometric information and receive health-related services regardless of time and place are developing. This paper proposes the healthcare system based on voice recognition function. Unlike conventional wearable healthcare systems, the proposed system provides real-time telemedicine and diagnosis and the direct connection with doctors is possible through it. And almost all functions of the application in it are operated with voice commands to lower user access barriers. Because it deals with personal information including medical information, encryption algorithms have been applied between all data movements. Finally, the healthcare system proposed in this paper is expected to increase convenience in health care and reduce the medical gap.

열안정형 카테콜-O-메틸전이효소 유전자의 클로닝

홍경만,최용복,정갑용,지은정,장현신,박현,백문기 圓光大學校 醫科學硏究所 1998 圓光醫科學 Vol.14 No.2

Catechol-O-methyltransferase (COMT; EC 2.1.1.6) is the enzyme which catalyzes the transfer of methyl group to the catecholamine neurotransmitters from its methyl donor S-adenosyl-L-methionine. Partially purified COMT from rat liver has been used to measure the concentration of catecholamines in the blood through solvent extraction and thin layer chromatography after converting ^3H-methyl derivatives of catecholamines. To improve this inconvenient and fluctuating method, an attempt was made to use COMT gene for the measurement of catecholamines. Specific primers, COMT5P (5'-TGC TCA GAG GTG CTT TGA AG-3') and COMT3P (5'-GGA GCC GCA GAA GGT CA G-3'), were used to amplify COMT gene from human placenta cDNA library. The amplified COMT gene through 35 cycles of polymerase chain reaction was cloned into T-vector and the nucleotide sequences are determined by automatic sequencer. Human COMT has two common variants, a thermostable high activity form which has valine at amino acid 158 and a thermolabile low activity form which Has methionine at the same position. The cloned COMT gene in this study has both variants. Clone 4 and 5 have valine at amino acid 158 (guanine at nucleotide 472) and clone 1-3 have methionine at this site (adenine at nucleotide 472). In addition to this, there are other DNA polymorphisms in COMT gene at nucleotide 101, 102 (amino acid 34, a structural mutation cysteine/serine) and nucleotide 186 (a silent mutation). All of the clones have cysteine at amino acid 34 (guanine and adenine at nucleotides 101 and 102) and cytosine at nucleotide 186 except clone 3 which has thymine at this nucleotide. Clone 4 and 5 are thermostable high activity variants, suggesting being more useful for the measurement of catecholamines after expressing the gene.

한국전통생활문화에 관한 고찰

이숙,홍윤호,신윤숙,신효식,한경미,최정미,김은정 全南大學校家政科學硏究所 2002 生活科學硏究 Vol.12 No.-

This study is objected on preliminary research for more scientific and wider studies on the Traditional Living Culture of Korea. For this purpose, studies about clothing, food, home management, Traditional Living Culture were reviewed and analysed. The results were as follows : First, Clothing culture studies concentrated on ritual clothings, Second, Food Culture studies included history of traditional food development and recipes. Third, in the field of home management value system, attitudes and parent-child relations were studied,

Dietary Aloe Reduces Adipogenesis via the Activation of AMPK and Suppresses Obesity-related Inflammation in Obese Mice

Shin, Eun-Ju,Shin, Seul-Mee,Kong, Hyun-Seok,Lee, Sung-Won,Do, Seon-Gil,Jo, Tae-Hyung,Park, Young-In,Lee, Chong-Kil,Hwang, In-Kyeong,Kim, Kyung-Jae The Korean Association of Immunobiologists 2011 Immune Network Vol.11 No.2

Background: Metabolic disorders, including type II diabetes and obesity, present major health risks in industrialized countries. AMP-activated protein kinase (AMPK) has become the focus of a great deal of attention as a novel therapeutic target for the treatment of metabolic syndromes. In this study, we evaluated whether dietary aloe could reduce obesity-induced inflammation and adipogenesis. Methods: Male C57BL/6 obese mice fed a high-fat diet for 54 days received a supplement of aloe formula (PAG, ALS, Aloe QDM, and Aloe QDM complex) or pioglitazone (PGZ) and were compared with unsupplemented controls (high-fat diet; HFD) or mice fed a regular diet (RD). RT-PCR and western blot analysis were used to quantify the expression of obesity-induced inflammation. Results: Aloe QDM complex downregulated fat size through suppressed expression of scavenger receptors on adipose tissue macrophages (ATMs) compared with HFD. Both white adipose tissue (WATs) and muscle exhibited increased AMPK activation through aloe supplementation, and in particular, the Aloe QDM complex. Obesity-induced inflammatory cytokines (IL-$1{\beta}$ and -6) and $HIF1{\alpha}$ mRNA and protein were decreased markedly, as was macrophage infiltration by the Aloe QDM complex. Further, the Aloe QDM complex decreased the translocation of NF-${\kappa}B$ p65 from the cytosol in the WAT. Conclusion: Dietary aloe formula reduced obesity-induced inflammatory responses by activation of AMPK in muscle and suppression of proinflammatory cytokines in the WAT. Additionally, the expression of scavenger receptors in the ATM and activation of AMPK in WAT led to reduction in the percent of body fat. Thus, we suggest that the effect of the Aloe QDM complex in the WAT and muscle are related to activation of AMPK and its use as a nutritional intervention against T2D and obesity-related inflammation.

The Regulation of Fatty Acid Oxidation in Human Preeclampsia

Shin, Eun-Kyeong,Kang, Hee Young,Yang, Hyun,Jung, Eui-Man,Jeung, Eui-Bae SAGE Publications 2016 REPRODUCTIVE SCIENCES Vol.23 No.10

<P>Preeclampsia (PE) is a pregnancy disorder characterized by high blood pressure, placental oxidative stress, and proteinuria. In a GeneFishing experiment using human preeclamptic placenta, expression of acyl-coenzyme A dehydrogenase very long chain (ACADVL), which is involved in fatty acid -oxidation (FAO), was detected. To investigate the correlation between PE and FAO, this study subjected in vitro BeWo cells and in vivo pregnant mice to oxidative stress induced by hypoxia. Hypoxic condition, which oxygen supply is insufficient in cells and placenta, created a similar state to placental oxidative stress in PE, as evidenced by increased hypoxic (oxoguanine DNA glycosylase 1, hypoxia inducible factor 1 alpha subunit) and preeclamptic markers (soluble fms-like tyrosine kinase 1) both in vitro and in vivo. Increased expression of FAO-related genes (ACADVL, enoyl-coenzyme A hydratase/3-hydroxyacyl coenzyme A dehydrogenase) was observed in these models as well as in cases of preeclamptic preterm labor. In the in vivo liver model, messenger RNA expression of gluconeogenesis-related genes increased. Consequently, these results suggest that expression of FAO-related genes is regulated by hypoxic conditions and onset time of PE and affects maternal gluconeogenesis during pregnancy in patients with PE.</P>

Structural Study of Antisense Dimers,Modified Adenosine-Thymidine Phosphorothioate

JUNG, KYEONG-EUN,YANG, MIRIM,LEE, KWANGJUN,LIM, HONG,JUNG, JIHYUN,KOO. BONJUNG,JEONG, LAK SHIN,SHIN, DONG-HOON,LEE, CHUL-HOON,CHO, YOUL-HEE,LIM, YOONGHO 한국미생물 · 생명공학회 2000 Journal of microbiology and biotechnology Vol.10 No.6

Antisense molecules are structurally simple linear oligomers of nucleotides. They can recognize a complementary sequence by base pairing, therefore, antisense drugs composed of 15-16 bases are potentially useful, unlike drugs such as protein agonists, antagonists, and inhibitors. Since antisense oligomers are classified as nucleotides, they are subject to attack by nucleases. In order to be antisense drugs resistant to degradation by nucleases, the structural modifications in the linkages, bases, and sugars to satisfy this requirement are considerable. We attempted in this study, to synthesize 16-mer antisenses with a modified linkage and adenosine. When studying on the three-dimensional structure of the oligomer, however, the existence of isomers may complicate the interpretation of the NMR data. Therefore, an attempt was made to eliminate the above problem, thus, two dimers were synthesized and their structural studies were carried out.

VNIR 초분광 분석을 이용한 복숭아 ‘마도카’ 과실의 가용성 고형물 함량 예측

장경은(Kyeong Eun Jang),김경호(Gyeong Ho Kim),김민화(Minhwa Kim),박정인(Jung In Park),이은진(Eun Jin Lee),신해인(Hae In Shin),한가람(Ga Ram Han),이근재(Geun Jae Lee),조정건(Jung Gun Cho),정재훈(Jae Hoon Jeong),이슬기(Seul Ki Lee),김진국 한국원예학회 2021 한국원예학회 학술발표요지 Vol.2021 No.10

지구온난화 및 병충해 발생 증가 등의 환경적인 요인은 안정적인 과실생산에 큰 위협이 되고 있다. 많은 과수 작물 중에서도 복숭아는 숙기 예측이 어렵고 쉽게 물러지는 특징이 있다. 특히 자연재해에 약하며 최근에는 이상기후로 인해 개화기와 수확기의 예측이 어려워지면서 많은 농가들이 피해를 받고 있다. 최근 농업 현장에서는 비파괴 센싱 기술 중에서도 초분광 영상 기술을 과실 생육 과정에 활용하여 수확 적기와 품질 예측을 통해 고품질의 과실을 생산하고자 많은 노력을 기울이고 있다. 본 연구는 복숭아 ‘마도카’ 과실의 수확기를 예측하고 수확 후 생육특징과 초분광 영상과의 상관관계와 그에 따른 신뢰도를 알아보고자 수행하였다. 초분광영상 취득은 SPECIM-IQ(SPECIM, SPECTRAL IMAGING LTD., Oulu, Finland) 카메라를 사용하였다. 수확 시기별 과실 30개씩을 7월 13일부터 3 ~ 5일 간격으로 6번 수확 후 180개 복숭아 과실에 대한 스펙트럼 데이터를 이용하여 모델링을 실시하였다. 복숭아 ‘마도카’를 필드, 마도카 전체 과실, 마도카 정중앙 과실의 과피와 과육 부분의 초분광 영상 스펙트럼(400 ‒ 1000nm)을 수집하고 영상을 분석한 결과, Core 과피 가용성 고형물 함량 예측 모델의 정밀도는 전처리하지 않은 경우 R² = 0.74, 평활화처리, 1차 미분, 2차 미분시 정밀도는 R² = 0.56 ~ 0.88로 나타났다. 복숭아 Core 과피의 고형물 함량 모델의 validation 결과, 전처리 전의 정밀도는 R² = 0.96을 보였고, 평활화처리, 1차 미분, 2차 미분시 예측 정밀도는 R² = 0.93 ~ 0.97의 예측성능을 보였다. 복숭아 과실 전체의 고형물 함량에 대한 모델은 전처리 전의 정밀도는 R² = 0.65를 보였고, 평활화, 1차 미분, 2차 미분시 예측 정밀도는 R2 = 0.73 ~ 0.88의 예측성능을 보였다. 복숭아 과실 전체의 고형물 함량 모델의 validation 결과, 전처리 전의 정밀도는 R² = 0.96을 보였고, 평활화, 1차미분, 2차 미분시 예측 정밀도는 R² = 0.97 ~ 0.98의 예측성능을 보였다. 따라서, VNIR 초분광 영상을 활용한 복숭아 과실의 가용성 고형물 함량 예측이 가능할 것으로 판단되었으며, 복숭아 숙도 선정에 필요한 다양한 수확 지표들에 대한 초분광 영상 적용성을 검토해야 할 것으로 판단되었다.

연구논문(硏究論文) : 동아리활동에 따른 진로결정자기효능감이 진로성숙도에 미치는 영향

신은경 ( Eun Kyeong Shin ),윤경 ( Kyeong Youn ),김진강 ( Jin Kang Kim ) 한국관광산업학회 2016 Tourism Research Vol.41 No.1

본 연구는 혁신고와 일반고 학생들의 동아리활동이 진로결정자기효능감과 진로성숙도에 어떠한 영향을 미치는지 알아보는데 목적이 있다. 연구의 목적을 달성하기 위해 국내 일반고와 혁신고에 다니고 있는 고등학생을 모집단으로 설정하였으며, 표본집단은 광주광역시 소재 일반고와 혁신고를 선정하여 설문지를 이용하여 분석하였다. 표본추출방법은 무작위추출법을 이용하였으며 조사는 설문지방법을 이용하였다. 또한 설문지는 총 500부를 배포하여 450부를 회수하였으며 이중 설문응답에 대한 누락이나 일 방향으로 기입한 174부를 제외한 총 276부를 최종 유효 표본으로 분석에 사용하였다. 분석결과 다음과 같은 연구결과가 도출되었다. 첫째, 진로결정자기효능감 요인 중에서 미래계획 요인이 확신성에 긍정적인 정(+)의 영향을 주는 중요한 요인으로 인식되고 있음을 알 수 있으나, 목표선택 요인과 직업정보 요인은 확신성에 영향을 주지 않은 요인으로 나타났다. 둘째, 동아리활동의 장래 직업과의 관련성에 따른 진로결정자기효능감(목표선택, 직업정보, 미래계획) 모두는 유의수준 p=0.05보다 작기 때문에 통계적으로 유의한 차이가 있는 것으로 나타났다. 진로성숙도는 결정성, 확신성, 목적성, 준비성과 독립성 5가지요인으로 분류하였다. 하지만 구성요인 중 결정성 요인, 준비성 요인, 독립성 요인의 유의수준 p=0.05보다 작기 때문에 통계적으로 유의한 차이가 있는 것으로 나타났으나 진로성숙도의 구성요인 중 목적성 요인과 확신성 요인은 유의적인 차이가 없는 것으로 나타났다. 셋째, 진로결정자기효능감과 진로성숙도와의 관계를 살펴본 결과 진로결정자기효능감의 목표선택은은 진로성숙도의 준비성과 독립성에, 진로결정자기효능감의 직업정보는 진로성숙도의 독립성에, 진로결정자기효능감의 미래계획요인은 진로성숙도의 모든 요인(결정성, 준비성, 독립성, 목적성, 확신성)에 유의한 영향을 미치는 것으로 나타났다. 따라서 동아리활동은 학생들에 대한 인지적 미래계획을 위해 진로교육 및 진로설계 과정에서 동아리활동의 중요성을 담은 진로체험 및 총체적인 진로교육 시스템 마련이 필요할 것이다. 또한 향후 연구에서는 진로결정자기효능감과 진로성숙 및 결정에 관한 측정척도에 심도 있는 검증과정이 필요한 것으로 확인되었다. The purposes of this study were to identify the differences of the effects of club activities on career decision-making self-efficacy and career maturity between educational revolution high school and general high school and to acknowledge the meaningful effect of career decision to career maturity of high school students. Students of educational revolution high school and general high school in South korea are selected for a population, and an educational revolution high school students and a general high school students in Gwangju are randomly sampled for this study. Totally 500 questionnaires are distributed and 450 of them are collected. 276 questionnaires are used for the examination excluded unfaithful 174. The major findings of this study are as follows; Multiple regression analysis is used for the identification of the effect of career decision-making self-efficacy to career maturity through the club activities, and not the goal selection and the job information but only the future plan of career decision-making self-efficacy factors appears positive effect to conviction of career maturity factors. The future plan of career decision-making self-efficacy factors appears positive effect to determinacy of career maturity factors, so it is very difficult that students make high determinacy on career decision-making self-efficacy and prepare for future career for their whole life. Career decision-making self-efficacy appears meaningful effect to readiness of career maturity factors, the goal selection and the job information of career decision-making self-efficacy factors appear positive effect to readiness. Work experiences that contain educational effects of student club activity and total career education system should be provided on students for practical future plan through the whole course of life education and mapping their career paths. In the hereafter research, qualification process of measure on career maturity and career decision is needed.