Suppression of lung inflammation by the methanol extract of <i>Spilanthes acmella</i> Murray is related to differential regulation of NF-κB and Nrf2

Kim, Kyun Ha,Kim, Eun Jung,Kwun, Min Jung,Lee, Ji Yeon,Bach, Tran The,Eum, Sang Mi,Choi, Jun Yong,Cho, Sayeon,Kim, Sang-Jun,Jeong, Seung-Il,Joo, Myungsoo Elsevier 2018 Journal of Ethnopharmacology Vol.217 No.-

<P><B>Abstract</B></P> <P><B>Ethnopharmacological relevance</B></P> <P>Although <I>Spilanthes acmella</I> has been used to relieve inflammation, fever, pain, or infection in traditional Asian medicine, experimental evidence supporting these functions is scarce. Here, we examined an anti-inflammatory function and a possible underlying mechanism of <I>S. acmella</I> Murray (SAM).</P> <P><B>Materials and method</B></P> <P>The methanol extract of SAM was fingerprinted by HPLC. C57BL/6 mice were administered with a single intratracheal (i.t.) LPS and 2 h later with a single i.t. SAM. The effect of SAM on lung inflammation was assessed by histology, semi-quantitative RT-PCR, and MPO assay of lung tissue. The effects of SAM on a pro-inflammatory factor NF-κB and an anti-inflammatory factor Nrf2 were analyzed by immunoblotting of nuclear proteins and by semi-quantitative RT-PCR analysis of mRNA of the genes governed by these transcription factors. V5-Nrf2 was precipitated by an anti-V5 antibody and the ubiquitinated V5-Nrf2 was revealed by immunoblotting of HA-tagged ubiquitin.</P> <P><B>Results</B></P> <P>The i.t. SAM robustly diminished a neutrophilic lung inflammation induced by i.t. LPS treatment of mice. In RAW 264.7 cells, SAM suppressed the nuclear localization of NF-κB and the expression of NF-κB-dependent cytokine genes. SAM increased the level of Nrf2 in the nucleus and the expression of Nrf2-dependent genes while suppressing ubiquitination of Nrf2.</P> <P><B>Conclusion</B></P> <P>Our results suggest that SAM can suppress a neutrophilic inflammation in mouse lungs, which is associated with suppressed NF-κB and activated Nrf2. Our results provide experimental evidence supporting the anti-inflammatory function of <I>S. acmella</I>.</P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

미량원소의 엽면시비가 토마토의 초기 생육에 미치는 영향 : Lycopersicum esculemtum Mill

전하준,최문환,김민 대구대학교 생명과학연구소 2002 생명과학연구 Vol.1 No.2

토마토를 이용하여 미량원소 엽면시비제인 Saponin과 EVA를 각각 1000㎖·ℓ^-1와 3000㎖·ℓ^-1의 농도로 처리하여, 생육초기에 미치는 효과를 조사하였다. 토마토의 초장, 엽수, 경경 그리고 엽면적에 미치는 엽면 시비제의 생육촉진효과의 일정한 경향을 관찰할 수는 없었지만, 생육단계에 따라서 일부 처리에 있어서는 엽면시비에 의한 수치의 증가를 관찰할 수 있었다. 그러나, 각각의 조사항목에 대한 수치의 변화는 서로 연관성이 있을 것으로 생각되며, 이러한 수치의 변화는 미량요소에 의한 효과인지 아니면 성분표시가 되어있지 않은 식물생장조절물질 같은 성분의 효과인지에 대해서는 앞으로도 계속적인 실험을 통하여 확인할 필요가 있을 것으로 생각되었다. 본 실험의 결과에서 생산자의 무분별한 엽면시비제의 사용은 그 효능과 효과에 의문을 제기할 수 있는 가능성이 충분히 있을 수 있으므로, 그 사용에 있어서는 작물에 따라서 성분, 사용량, 사용방법을 충분히 파악하여 사용하는 것이 경제적일 뿐만 아니라 작물생육을 위해서도 유익할 것으로 생각되었다. This study was conducted to investigate the effects of foliar application of microelements on the earlier growth of table variety tomato. There were no significant effects of foliar application on plant height, leaf number, stem diameter, leaf area in tomato, but it seemed that there were some possibility for growth promote effect in leaf number and leaf area by foliar spray of EVA 1000㎖·ℓ^-1 and Saponin 1000㎖·ℓ^-1 treatment at some growth stages. Foliar spray of microelements may have some efficient effects for plant growth or differentiation, they have to confirmed the optimum concentration, treatment times and components with plants.

영양소제거 공정의 기질 정량관계에 대한 온도와 기질부하의 영향

하준수,최의소,박재홍,길경익,김성원 대한상하수도학회 2003 상하수도학회지 Vol.17 No.6

This research has been performed to evaluate the stoichiometric relationship between removed COD and nitrogen or phosphorus. A modified A²/O process, which was equipped with an external nitrification tank and a switching reactor, was operated with a capacity of 50㎥/d. From the results, it has been concluded that the stoichiometric values were varied by temperature condition or input COD loads. In case of denitirfication, the requirement g COD for removed nitrate nitrogen ranged 0.13 to 10.5g, and the compensation coefficient of temperature was evaluated as 1.028. For phosphorus removal, the required COD was about 7.5gCOD_(consumed)/gP_(released) and 0.54gPHACOD_(storage)/gP_(uptake). During rainy season, as it appeared a lower organic substrate loads, the PHA(Poly Hydroxy Alkanoate) contents of biomass was reduced. From the relationship between influent BOD, and the sum of removed TN and TP, the ratio for rainy and winter season was about 4.8 and 5.8g BOD/g (TN+TP) each others. Therefor, the substrat ratios for nitrogen and phosphorus removal appeared to be affected by temperature and influent organic substrate loads.

朝鮮 實學派의 道德類 私傳 硏究

崔俊夏 한국중국문화학회 1992 中國學論叢 Vol.1 No.-

식도를 포함한 전위장관을 침범한 전이성 악성 흑색종 1예

최정영,김준기,신성하,고영춘,조상욱,서강석,강명원,임연근,여향순,김경수 全南大醫大 2001 전남의대학술지 Vol.37 No.1

특발성 경련질환으로 오인된 원발성 부갑상선기능저하증 1례

신성하,최정영,김준기,고영춘,조상욱,김순호,임중규 全南大醫大 2001 전남의대학술지 Vol.37 No.1

소아에서 출혈을 동반한 단일 연소성 용종의 내시경적 절제술

김준기,조상욱,신성하,고영춘,최정영,서강석,강명원,임연근,여향순,김경수 全南大醫大 2001 전남의대학술지 Vol.37 No.3

고온 유리의 프레스 성형 공정 시뮬레이션

지석만,최주호,김준범,하덕식 한국 항공대학교 항공산업기술연구소 2001 航空宇宙産業技術硏究所 硏究誌 Vol.11 No.-

This paper addresses a method for numerical simulation in the pressing process of hot glass. Updated Lagrangian finite element formulations are employed for the flow and energy equations to accommodate moving meshes. The model is assumed axi-symmetric and creep flow is assumed due to the high viscosity. Commercial software ANSYS is used to solve the coupled flow and energy equations. Moving contact points as well as free surface during the pressing are effectively calculated and updated by utilizing API functions of CAD software Unigraphics. The mesh distortion problem near the wall is overcome by automatic remeshing, and the temperatures of the new mesh are conveniently interpolated by using a unique function of ANSYS. The developed model is applied to the pressing process of TV glasses. In conclusion, the presented method shows that the pressing process accompanying moving boundary can be simulated by effectively combining general purpose softwares without resorting to special dedicated codes.

황금배 品種의 單一品種 果園을 위한 콩배 受粉樹 移用에 관한 硏究

沈慶久,河有美,李俊馥,崔勇洵 성균관대학교 생명과학자원연구소 1994 生命資源科學硏究 Vol.1 No.1

Fauriei pear(Pyrus fauriei Nakai) potentially useful as pollenizers in single-cultivar `Hwangkuembae' orchard was evaluated for their flowering times and pollen germination(%). Their efficiency in the setting of fruits following controlled pollinations on 'Hwangkuembae' at Fruit Tree Research Institute in Suwon was also examined. The obtained results are as followes ; (1) Flowering times of SKK 1 was from April 21 and that of them corresponded to that of 'Hwangkuembae'. (2) Pollen germination (%) of SKK 1 using as pollenizer of 'Hwangkuembae' was over 90%. Therefore Based on these factors, folwering times and pollen germination, SKK 1 was found to be promising as pollenizer for single-cultivar 'Hwangkuembae' orchard. (3) Percentage of fruit setting in 'Hwangkuembae' pollinated with Fauriei pear showed 71.9%. And there was no statistically significant difference in the fruit diameter(cm), fruit height(cm), fruit index, fruit weight (g), soluble solid(˚BX), acidity(pH), and fruit hardness (kg/㎠) of 'Hwangkuembae' pollinated with Fauriei pear (4) Percentages of fruit setting in 'Hwangkuembae' pollnated with Fauriei pear corresponding to flowering times of 'Hwangkuembae', was 871.9% and no statistically significant difference in fruit quality. On the bases of flowering times, pollen germination(%), efficient fruit set(%), and no statistically significant difference in fruit quality, Fauriei pear(Pyrus fauriei Nakai) is promising as pollenizers for single-cultivar 'Hwangkuembae' orchard.

장티푸스 진단에 있어서 Vi-Indirect Fluorescent Antibody Test의 민감도 및 특이도

장경희,류동렬,박성하,박병규,이준구,염준섭,최영화,송영구,김현숙,정윤섭,김준명 대한감염학회 1998 감염 Vol.30 No.4

목 적 : 장티푸스는 체액의 세균배양검사에서 Salmonella typhi의 분리로 확진하지만 조기진단을 위해서는 혈청학적인 검사가 도움이 된다. 저자 등은 1989년 이후 장티푸스진단에 S. typhi의 Vi항원을 이용한 간접형광항체법 (Vi-indirect fluorescent antibody test : Vi-IFAT)을 도입하였다. 이에 지난 7년간의 검사결과를 토대로 Vi-IFAT의 민감도 및 특이도를 규명하고자 하였다. 방 법 : 1989년부터 1996년까지 연세대학교 의과대학 세브란스 병원에 발열을 주소로 내원하여 Vi-IFAT가 의뢰된 환자 중 발열의 원인이 확진된 744명의 환자를 대상으로 하였으며, Vi-IFAT에 있어서 양성반응은 1:64이상으로 하였다. 결 과 : 1) 세균배양검사에서 확진된 장티푸스 환자 178예 중 168예에서 양성반응을 보여 민감도는 94.4%이었다. 항체가는 1:64가 89예, 1:128이 36예, 1:256이 27예, 그리고 1:512가 16예이었다. 위음성이었던 10예의 항체가는 1:32가 7예, 1:16이하가 3예이었다. 2) 발열을 주소로 내원한 환자 중 장티푸스 이외의 다른 질환으로 확진된 566예 중 538예에서 음성반응을 보여 특이도는 95.1%이었다. 항체가는 1:32가 117예, 1:16이하가 421예이었다. 위양성이었던 28예의 항체가는 1:64가 23예, 1:128이 5예이었으며, 장티푸스 이외의 살모넬라증이 4예, 요로계감염이 10예, 소화기계감염이 6예, 호흡기 계감염이 5예, 악성 종양이 2예, 그리고 바이러스 감염이 1예이었다. 3) Vi-IFAT 양성인 196예 중에서 장티푸스는 168예로 양성예측율은 85.7%이었으며, Vi-IFAT 음성인 548예 중 장티푸스 이외의 발열질환은 538예로 음성예측율 98.2%이었다. 4) 배양검사와 Widal test 사이의 일치율 (Kappa value)는 0.28로 poor agreement를 보였으며 Vi-IFAT와 Widal test 사이의 Kappa value는 0.74로 good agreement를 보였다. 그러나 배양검사와 Vi-IFAT 사이의 Kappa value는 0.86으로 excellent agreement를 보여 일치율이 가장 높은 검사는 배양검사와 Vi-IFAT라 할 수 있겠다. 5) 장티푸스 환자에 있어서 Vi-IFAT의 양성율은 발열기간이 1중 미만인 환자에서는 68%, 1내지 2주인 환자에서는 89.5%, 2주이상인 환자에서는 100%이었다. 결 론 : Vi 항원을 이용한 간접형광항체법 (Vi-IFAT)은 장티푸스 진단에 높은 민감도 및 특이도를 가지며, 또한 조기진단에 있어서도 임상적으로 유용함을 확인할 수 있었다. Background : Typhoid fever is diagnosed by culture or serological study. The confirmative diagnosis of typhoid fever is made by culture of the causative organism usually from body fluids. Serological test is a supportive diagnositic tool, which is useful for early diagnosis. In Severance Hospital, Vi-indirect foluorescent antibidy test(Vi-IFAT) using the Vi-antigen of Salmonella typhi has been used in the diagnosis of typhoid fever since 1989. We investigated the test results from the past 7 years, in order to clarify the sensitivity and specificity of Vi-IFAT. Methods : A retrospective study was done on patients whose chief complaint was fever and who were tested using Vi-IFAT in the Severance Hospital from 1989 to 1996. The positive value for Vi-IFAT was defined as 1:64 or higher. Results : The sensitivity and specificity of Vi-IFAT for typhoid fever was 94.4% and 95.1%, respectively. The positive and negative predictive values were 85.7% and 98.2% respectively. Positive rates of Vi-IFAT after fever onset increased with time and 68% were positive before the first week. From the first to the second week, 89.5% were positive and after the second week, 100% were positive. Conclusion : Vi-IFAT is not only a valuable serologic test for the diagnosis of typhoid fever, but also useful in the early diagnosis of the disease.